BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0370.0017.p (433 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0402 [O] COG0450 Peroxiredoxin 265 1e-70 sll0108 [P] COG0004 Ammonia permease 258 9e-69 AF1746 [P] COG0004 Ammonia permease 246 5e-65 MA4207 [P] COG0004 Ammonia permease 231 2e-60 alr0991 [P] COG0004 Ammonia permease 213 7e-55 >STM0402 [O] COG0450 Peroxiredoxin Length = 200 Score = 265 bits (676), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 0/417 (0%), Positives = 0/417 (0%), Gaps = 35/417 (8%) Query: 5 LESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFYI 64 + + + F + ++ + + Sbjct: 24 DKFNFKQHTNGKTTVLFFWPMDFTFVCPSELIAFDKRYEEFQKRGVEVVGVSFDSEFVHN 83 Query: 65 FGFGLMYGKDAGGLVGTSGFMLGGDLSNLHFTISVD------TFWLFQAAFTIAVISIVS 118 K + + Sbjct: 84 AWRNTPVDKGG-----------------IGPVKYAMVADVKREIQKAYGIEHPDEGVALR 126 Query: 119 GAVAERINFRAYLLYVIFMTGLIYPLXXXXXXXXXXXXXXXXXDFAGSAVIHALGGFSAL 178 G+ N V + G ++ Sbjct: 127 GSFLIDANGIVRHQVVNDLPLGRNIDEMLRMVDALQFHEEHGDVCPAQWEKGKEGMNASP 186 Query: 179 AAAIVIGPRKGKFNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHI 238 Sbjct: 187 246 Query: 239 AITTMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAV 298 Sbjct: 247 306 Query: 299 SGLLMMAATNWLEA-RRIDDPVGAFPVHAVSGAWGTVAVGLFATD-----------GGLL 346 Sbjct: 307 366 Query: 347 YGGGWHQLGVQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGM 403 Sbjct: 367 423 >sll0108 [P] COG0004 Ammonia permease Length = 507 Score = 258 bits (660), Expect = 9e-69, Method: Compositional matrix adjust. Identities = 158/412 (38%), Positives = 238/412 (57%), Gaps = 10/412 (2%) Query: 1 MNVTLESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTL 60 +N + +L G ++ IW++++A +++ M GF +LE G R KN+VNI+ K + T+ Sbjct: 77 VNENVATLQGTLNAIWILIAAILVIFMNAGFGMLETGLCRQKNAVNILTKNLIVFALATI 136 Query: 61 IFYIFGFGLMYGKDAGGLVGTSGFMLGGDLSNLHFT-----ISVDTFWLFQAAFTIAVIS 115 ++ GF LM+G VG GF L GD +N + + V F+LFQ AF+ + Sbjct: 137 AYWAIGFSLMFGSSGNPFVGFGGFFLSGDHTNYGLSPFPEGLPVAVFFLFQVAFSATAAT 196 Query: 116 IVSGAVAERINFRAYLLYVIFMTGLIYPLXXXXXXXXXX-XXXXXXXDFAGSAVIHALGG 174 IVSGAVAERI F +L++ + + G+ YP+ DFAGS V+H++GG Sbjct: 197 IVSGAVAERIKFNEFLIFSVLLVGIAYPITGHWVWDAGGWLYTMGFMDFAGSTVVHSVGG 256 Query: 175 FSALAAAIVIGPRKGKFNQAGNSTVALP-SNLPLASVXXXXXXXXXXXXXXXSTLSATDA 233 ++ALA A ++GPR GKF A+P N+ A + S L+A D Sbjct: 257 WAALAGAFLLGPRLGKFVDGRPG--AIPGHNMGFAMLGCLILWIGWFGFNPGSQLAA-DQ 313 Query: 234 RIGHIAITTMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAI 293 +IA+TT L+A+AGG + + + D VING LAGLVGITAGCA VS ++ Sbjct: 314 ACAYIAVTTNLAASAGGLTATFTSWLKDGKPDLTMVINGVLAGLVGITAGCAGVSYWGSV 373 Query: 294 LIGAVSGLLMMAATNWLEARRIDDPVGAFPVHAVSGAWGTVAVGLFATDGGLLYGGGWHQ 353 +IG ++G+L++ + + + +IDDPVGA VH V+G WGT+AVG F + GL YGGG +Q Sbjct: 374 IIGGIAGILVVYSVAFFDKIKIDDPVGAISVHLVNGVWGTLAVGFFNMEKGLFYGGGINQ 433 Query: 354 LGVQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIA 405 L +QI+G++ + A+ ++ ++ + + +R S EEE IGLDI HGM A Sbjct: 434 LIIQIVGILAIGAFTAIFSFVVWAILKQTMGIRVSGEEEMIGLDIGEHGMEA 485 >AF1746 [P] COG0004 Ammonia permease Length = 468 Score = 246 bits (628), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 155/417 (37%), Positives = 221/417 (52%), Gaps = 29/417 (6%) Query: 12 VDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFYIFGFGLMY 71 VD +W ++ +++ M+ GFA+LEAGF R KN N++MK D +G+L F+ GF LM Sbjct: 37 VDFVWALICGFLVMFMQAGFAMLEAGFSRAKNVANVMMKNLMDFAVGSLAFFAVGFALMM 96 Query: 72 GKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAVAERINFRAYL 131 G D G+ GT+G+ L G+ + +S W F F +IVSG++AER F YL Sbjct: 97 GADWQGIAGTTGWFLAGE----SYDVSTIELWFFMLVFAATAATIVSGSIAERPKFSVYL 152 Query: 132 LYVIFMTGLIYPLX----------XXXXXXXXXXXXXXXXDFAGSAVIHALGGFSALAAA 181 +Y ++ +IYP+ DFAGS V+HALGG+ ALAA Sbjct: 153 VYSAVVSAVIYPIYGHWLWGGGWLSSSEFMVKLGGGYGALDFAGSGVVHALGGYIALAAV 212 Query: 182 IVIGPRKGKFNQAGNSTVALPSNLPLASVXXXXXXXXXXXXXXXSTLSATDARIGHIAIT 241 +++GPR GK++ GN NL A + STLSA + R+ IA Sbjct: 213 MLLGPRLGKYDSDGNPRAIPGHNLAFAVIGTFILWFGWFGFNAGSTLSAHELRVSIIASN 272 Query: 242 TMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVSDGAAILIGAVSGL 301 T L+AAAG + T R + D NG++AGLV ITA CA+V ++++IG ++G Sbjct: 273 TNLAAAAGAVTAMAITWLRNGKPDVGMTCNGAVAGLVAITAPCAWVQPWSSVVIGTIAGF 332 Query: 302 LMMAATNWLEARRIDDPVGAFPVHAVSGAWGTVAVGLFATDG-------------GLLYG 348 + WLE R +DD VGA PVH SG WG +A+G+FA DG GLLY Sbjct: 333 IATYGYWWLEKRGLDDVVGAIPVHGFSGTWGLIALGIFA-DGSYGLYATESPLVTGLLY- 390 Query: 349 GGWHQLGVQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEEIGLDISYHGMIA 405 G W VQ++ + AW F T + ++ K++ +R S EEE +GLDI+ H +A Sbjct: 391 GNWGFFIVQLISAIVNFAWAFGTGFALFWILKKVIGIRVSPEEEMLGLDIAEHAAVA 447 >MA4207 [P] COG0004 Ammonia permease Length = 469 Score = 231 bits (589), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 158/433 (36%), Positives = 230/433 (53%), Gaps = 38/433 (8%) Query: 3 VTLESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIF 62 V +E + + +W++L++ ++ M GF+L+E G R KN+ NI+MK F I +G LI+ Sbjct: 36 VAIEGVQTALTFVWLLLASGLVFFMHAGFSLVETGLTRSKNTANILMKNFMTIVLGILIY 95 Query: 63 YIFGFGLMYGKDAGGLVGTSGFMLGGDLSNLHFTISVDTFWLFQAAFTIAVISIVSGAVA 122 + G+G+MYG DA GL+GT F L G + L W FQ F +IVSGA+A Sbjct: 96 WAVGWGVMYGADAAGLIGTDQFFLSGADNALW------NGWFFQMVFAATGATIVSGAMA 149 Query: 123 ERINFRAYLLYVIFMTGLIYP-------------LXXXXXXXXXXXXXXXXXDFAGSAVI 169 ER NF+AYL+Y I M +IYP L DFAGS V+ Sbjct: 150 ERTNFKAYLIYCIMMVAVIYPVYGHWVWSGADLALLTGADSPIVQAIGVPSHDFAGSGVV 209 Query: 170 HALGGFSALAAAIVIGPRKGKFNQAGNSTVALP-SNLPLASVXXXXXXXXXXXXXXXSTL 228 H++GG+SALA +++G R GKF V +P NL +A + STL Sbjct: 210 HSIGGYSALAGVLLVGARIGKFKDG--KAVPIPGHNLTIAFLGTLILALGWIGFNGGSTL 267 Query: 229 SATDARIGHIAITTMLSAAAGGGITLIYTLFRYKRSDAPSVINGSLAGLVGITAGCAFVS 288 D + + + T L+AAAG +T++ T + + D NG L GLV ITA C V+ Sbjct: 268 DGNDPYMNLVVVNTFLAAAAGAVVTMLITWMKTGKPDPSLTANGLLGGLVAITAPCGSVN 327 Query: 289 DGAAILIGAVSGLLMMAATNWLE-ARRIDDPVGAFPVHAVSGAWGTVAVGLF-------- 339 + AAI+IGA++G+++ A + E ++DDPVGA VH G+WG ++VGLF Sbjct: 328 NWAAIIIGAIAGIIIYAGVMFNENTLKVDDPVGAIAVHGYCGSWGLISVGLFSIGIGNGI 387 Query: 340 -------ATDGGLLYGGGWHQLGVQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEE 392 A GL YGGG L +Q++ ++ W F ++ K++ +V +R SEEEE Sbjct: 388 LADAAYAAEVPGLFYGGGISLLLIQLISVLCSIVWAFGISFIIFKILDAVVGLRVSEEEE 447 Query: 393 EIGLDISYHGMIA 405 +GLDI HG+ A Sbjct: 448 IMGLDICEHGIRA 460 >alr0991 [P] COG0004 Ammonia permease Length = 359 Score = 213 bits (541), Expect = 7e-55, Method: Compositional matrix adjust. Identities = 0/432 (0%), Positives = 0/432 (0%), Gaps = 34/432 (7%) Query: 4 TLESLGGGVDTIWVVLSAAMILLMEGGFALLEAGFVRYKNSVNIIMKVFADITIGTLIFY 63 + + + + + + + + + Sbjct: 69 GGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMH 128 Query: 64 IFGFGLMYGKDAGGLVGTSGFMLGG--------DLSNLHFT------ISVDTFWLFQAAF 109 G +A T + ++ +++ + Sbjct: 129 RLGVRPEQA-EAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPG 187 Query: 110 TIAVISIVSGAVAERINFRAYLLYVIFMTGLIYPLXXXXXXXXXXXXXXXXXDFAGSAVI 169 ++ + + + +F +I Sbjct: 188 QRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREH 247 Query: 170 HALGGFSALAAAIVIGPRKGKFNQAGNSTVALP-SNLPLASVXXXXXXXXXXXXXXXSTL 228 A + + P + + Sbjct: 248 KAGEPVGYGGTWVSERDTRLGVVA--MGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDM 305 Query: 229 SATDARIGHIAITTMLSAAAGGGITLIYTLFRY-KRSDAPSVINGSLAGLVGITAGCAFV 287 D G + + Sbjct: 306 ICVDLGPQAQDKAGDPVILWGE-GLPVERIAEMT 364 Query: 288 SDGAAILIGAVSGLLMMAATNWLEARRIDDPVGAFPVHAVSGAWGTVAVGLFA------- 340 Sbjct: 365 424 Query: 341 -------TDGGLLYGGGWHQLGVQILGLVTLAAWGFATTWCGLKLIGKLVQVRSSEEEEE 393 Sbjct: 425 484 Query: 394 IGLDISYHGMIA 405 Sbjct: 485 496 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,030,537 Number of extensions: 1153336 Number of successful extensions: 5621 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 5484 Number of HSP's successfully gapped: 36 Length of query: 433 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 324 Effective length of database: 25,807,025 Effective search space: 8361476100 Effective search space used: 8361476100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 117 (49.7 bits)