BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0031.p (669 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value jhp1037 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and relat... 202 2e-51 HP1110 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and relate... 201 2e-51 STM0017 [K] COG3710 DNA-binding winged-HTH domains 201 3e-51 TM0017 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and relate... 201 3e-51 MK0083 [C] COG1013 Pyruvate:ferredoxin oxidoreductase and relate... 182 1e-45 >jhp1037 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, alpha subunit Length = 346 Score = 202 bits (513), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 0/279 (0%), Positives = 0/279 (0%), Gaps = 5/279 (1%) Query: 2 FMLEQMPVQAGTRMPMVMNLICRSISGPLNIHGDHSDLYFALNTGWPILMCRDPQSVYDM 61 ++ + L Sbjct: 93 RNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSA 152 Query: 62 NLMALKLAEHAKVRLPVMVASDGYFTSHQKRRVQAFAHREDVHKFVGEQPPVGFTDTLDR 121 ++L A S++ K + Sbjct: 153 PAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL-ADKHDLPRIVTI--QNPYS 209 Query: 122 NNPVTVGPYMNEPDYINNRYQQSVAMYNAGEVFEEIAQEFAELTGRYYPMIEQYRMEDAD 181 + + E + + G + + R R Sbjct: 210 LLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQ 269 Query: 182 VAVFLMNSASEIIKDVVDQLRLQGIKAGAISPNMIRPFPQKQIAEALKNVKAITVGDRAD 241 + + +D ++ + + N++++ + D Sbjct: 270 TQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSED 329 Query: 242 SVGGHGGNMVNEIKAALFTYGNTTTKVISR-IYGLGGKD 279 + Sbjct: 330 VLAEIEA- 367 >HP1110 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, alpha subunit Length = 346 Score = 201 bits (512), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 0/279 (0%), Positives = 0/279 (0%), Gaps = 5/279 (1%) Query: 2 FMLEQMPVQAGTRMPMVMNLICRSISGPLNIHGDHSDLYFALNTGWPILMCRDPQSVYDM 61 ++ + L Sbjct: 93 RNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSA 152 Query: 62 NLMALKLAEHAKVRLPVMVASDGYFTSHQKRRVQAFAHREDVHKFVGEQPPVGFTDTLDR 121 ++L A S++ K + Sbjct: 153 PAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL-ADKHDLPRIVTI--QNPYS 209 Query: 122 NNPVTVGPYMNEPDYINNRYQQSVAMYNAGEVFEEIAQEFAELTGRYYPMIEQYRMEDAD 181 + + E + + G + + R R Sbjct: 210 LLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQ 269 Query: 182 VAVFLMNSASEIIKDVVDQLRLQGIKAGAISPNMIRPFPQKQIAEALKNVKAITVGDRAD 241 + + +D ++ + + N++++ + Sbjct: 270 TQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSE- 328 Query: 242 SVGGHGGNMVNEIKAALFTYGNTTTKVISR-IYGLGGKD 279 Sbjct: 329 367 >STM0017 [K] COG3710 DNA-binding winged-HTH domains Length = 147 Score = 201 bits (512), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 0/293 (0%), Positives = 0/293 (0%), Gaps = 5/293 (1%) Query: 2 FMLEQMPVQAGTRMPMVMNLICRSISGPLNIHGDHSDLYFALNTGWPILMCRDPQSVYDM 61 Sbjct: 92 151 Query: 62 NLMALKLAEHAKVRLPVMVASDGYFTSHQKRRVQAFAHREDVHKFVGEQPPVGFTDTLDR 121 Sbjct: 152 --- 208 Query: 122 NNPVTVGPYMNEPDYINNRYQQSVAMYNAGEVFEEIAQEFAELTGRYYPMIEQYRMEDAD 181 Sbjct: 209 268 Query: 182 VAVFLMNSASEIIKDVVDQLRLQGIKAGAISPNMIRPFPQKQIAEALKNVKAITVGDRAD 241 Sbjct: 269 328 Query: 242 SVGGHGGNMVNEIKAALFTYGNTTTKVISRIYGLGGKDFYAEDGHHFFQLAMD 294 Sbjct: 329 -- 379 >TM0017 [C] COG0674 Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, alpha subunit Length = 346 Score = 201 bits (512), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 0/293 (0%), Positives = 0/293 (0%), Gaps = 5/293 (1%) Query: 2 FMLEQMPVQAGTRMPMVMNLICRSISGPLNIHGDHSDLYFALNTGWPILMCRDPQSVYDM 61 ++ + S + S D Sbjct: 92 SRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDS 151 Query: 62 NLMALKLAEHAKVRLPVMVASDGYFTSHQKRRVQAFAHREDVHKFVGEQPPVGFTDTLDR 121 L + Y + + V T Sbjct: 152 APAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG-VMRYLHLADKHDLPRIV--TIQNPY 208 Query: 122 NNPVTVGPYMNEPDYINNRYQQSVAMYNAGEVFEEIAQEFAELTGRYYPMIEQYRMEDAD 181 + + A+ G + ++ + Sbjct: 209 SLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGE 268 Query: 182 VAVFLMNSASEIIKDVVDQLRLQGIKAGAISPNMIRPFPQKQIAEALKNVKAITVGDRAD 241 + + +I + + P + + LK + ++ Sbjct: 269 QTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSE 328 Query: 242 SVGGHGGNMVNEIKAALFTYGNTTTKVISRIYGLGGKDFYAEDGHHFFQLAMD 294 V Sbjct: 329 DVLAEIE-- 379 >MK0083 [C] COG1013 Pyruvate:ferredoxin oxidoreductase and related 2-oxoacid:ferredoxin oxidoreductases, beta subunit Length = 346 Score = 182 bits (463), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 18/301 (5%), Positives = 48/301 (15%), Gaps = 21/301 (6%) Query: 364 LSPGHGACPGCGIFSGLELFFKGIEGDIVALYHTGCAMVTTTGYPYSAHKSTFIHNLFQN 423 G M P + + + Sbjct: 15 TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLA 74 Query: 424 GAATLSGVVEMFWERKRRGELDGLGLKEDFTFVMVTGDGG-MDIGMGPAIGAALRGHKMI 482 + + G + D + A+ +L+ + Sbjct: 75 KHGSREKL-----IIA----SKVSG-PSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 483 IVEYDNEGYMNTGAQQSYSTPLGHRTSTSSIGKTQQGKVTQHKDTAQIMAATNIPYVFTG 542 ++ + S ++ A + + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 543 CEAYPQDLLKKAAKAQWYAQNEGLVYGKILIACPLNWMSEDKDGTDIVSLAVESCFFPLY 602 L K + +L ++E + LA F Sbjct: 185 GVMRYLHLADKHDLPR---IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 603 EVEQGTTTITYNPEDKDKRVEVSAWLKTMGKTRHLLKPENEPALRAFESEVQRRWTRLKA 662 + + + + +T+ + A Sbjct: 242 TGKYLNGAKPAGARN---TLFSRFTRYSGEQTQKA----VAAYVDIARRHGLDPAQMALA 294 Query: 663 K 663 Sbjct: 295 F 295 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 51,307,895 Number of extensions: 2184742 Number of successful extensions: 13392 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 13271 Number of HSP's successfully gapped: 59 Length of query: 669 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 556 Effective length of database: 25,345,689 Effective search space: 14092203084 Effective search space used: 14092203084 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)