BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0025.p (257 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MTH1560 [P] COG1348 Nitrogenase subunit NifH (ATPase) 199 3e-51 NMA1633 [J] COG0042 tRNA-dihydrouridine synthase 189 5e-48 MA1633 [P] COG1348 Nitrogenase subunit NifH (ATPase) 189 5e-48 MTH643 [P] COG1348 Nitrogenase subunit NifH (ATPase) 188 8e-48 all1455 [P] COG1348 Nitrogenase subunit NifH (ATPase) 185 7e-47 >MTH1560 [P] COG1348 Nitrogenase subunit NifH (ATPase) Length = 268 Score = 199 bits (506), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 0/240 (0%), Positives = 0/240 (0%), Gaps = 3/240 (1%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRD--NHAVRAQDVIFEGFNGIYXXXXXXXXX 58 + G Sbjct: 58 RDILTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKG 117 Query: 59 XXXXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTS 118 + + + + + + Sbjct: 118 TLVDIGADKLALCREKLTVNRIMS-FRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAE 176 Query: 119 ADFMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVP 178 + + + + A + D + Sbjct: 177 SLKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTL 236 Query: 179 RSVTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEWG 238 Sbjct: 237 296 >NMA1633 [J] COG0042 tRNA-dihydrouridine synthase Length = 283 Score = 189 bits (479), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 94/239 (39%), Positives = 143/239 (59%), Gaps = 9/239 (3%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLR-------DNHAVRAQDVIFEGFNGIYXXXX 53 VMQ GCDPK DST L G++IP VL+ R D +A+ ++++F+ GIY Sbjct: 32 VMQVGCDPKRDSTRILAEGKFIPAVLEEHREQLRIGKDEYAINLKNIVFKSPGGIYLVEA 91 Query: 54 XXXXXXXXXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHV 113 ++T++ +LK + F ++D IYDVLGDVVCGGF++P+REG AE + Sbjct: 92 GGPEPGIGCAGRGVLTALQILKDLKAFSTYNIDVAIYDVLGDVVCGGFSMPIREGFAEEI 151 Query: 114 YTVTSADFMAIYAANNLFKGIQKYSQDGGALLGGVIANSI-NAPYAKDIVDDFVKRTDTQ 172 Y + S FM+IYAANN+ + +Q+ ++ G A L G+I NS + + + F KR T Sbjct: 152 YLICSGGFMSIYAANNIARAVQRLAKRGDAGLAGIICNSNGDEAFEHAFIPAFAKRLGTP 211 Query: 173 VVEYVPRSVTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPL-DTQEL 230 V++VPRS + EL G+ +E APDS++A+++RNLA+ I ++ +P P+ D EL Sbjct: 212 FVQFVPRSPVIQACELGGRPVVEYAPDSQEAEIFRNLAKAIIENDSRVIPTPINDLAEL 270 >MA1633 [P] COG1348 Nitrogenase subunit NifH (ATPase) Length = 283 Score = 189 bits (479), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 94/239 (39%), Positives = 143/239 (59%), Gaps = 9/239 (3%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLR-------DNHAVRAQDVIFEGFNGIYXXXX 53 VMQ GCDPK DST L G++IP VL+ R D +A+ ++++F+ GIY Sbjct: 32 VMQVGCDPKRDSTRILAEGKFIPAVLEEHREQLRIGKDEYAINLKNIVFKSPGGIYLVEA 91 Query: 54 XXXXXXXXXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHV 113 ++T++ +LK + F ++D IYDVLGDVVCGGF++P+REG AE + Sbjct: 92 GGPEPGIGCAGRGVLTALQILKDLKAFSTYNIDVAIYDVLGDVVCGGFSMPIREGFAEEI 151 Query: 114 YTVTSADFMAIYAANNLFKGIQKYSQDGGALLGGVIANSI-NAPYAKDIVDDFVKRTDTQ 172 Y + S FM+IYAANN+ + +Q+ ++ G A L G+I NS + + + F KR T Sbjct: 152 YLICSGGFMSIYAANNIARAVQRLAKRGDAGLAGIICNSNGDEAFEHAFIPAFAKRLGTP 211 Query: 173 VVEYVPRSVTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPL-DTQEL 230 V++VPRS + EL G+ +E APDS++A+++RNLA+ I ++ +P P+ D EL Sbjct: 212 FVQFVPRSPVIQACELGGRPVVEYAPDSQEAEIFRNLAKAIIENDSRVIPTPINDLAEL 270 >MTH643 [P] COG1348 Nitrogenase subunit NifH (ATPase) Length = 136 Score = 188 bits (477), Expect = 8e-48, Method: Compositional matrix adjust. Identities = 0/226 (0%), Positives = 0/226 (0%), Gaps = 6/226 (2%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVRAQDVIFEGFNGIYXXXXXXXXXXX 60 + E I + + + Sbjct: 31 RQRRSLKRGFLPRQKKVL-EKIRKIKKEGKTEGRPPVIRTHCRDMIVLPEMVGMTFGIHN 89 Query: 61 XXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVTSAD 120 + ++ Sbjct: 90 GKEFVEVKIQPEMIGCYFGEFA- 148 Query: 121 FMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYVPRS 180 Sbjct: 149 ---- 204 Query: 181 VTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLD 226 Sbjct: 205 250 >all1455 [P] COG1348 Nitrogenase subunit NifH (ATPase) Length = 295 Score = 185 bits (469), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 96/241 (39%), Positives = 140/241 (58%), Gaps = 5/241 (2%) Query: 1 VMQFGCDPKSDSTNTLRGGEYIPTVLDTLRDNHAVR---AQDVIFEGFNGIYXXXXXXXX 57 +M GCDPK+DST + + TVL + AV +V+ GF G+ Sbjct: 37 IMIVGCDPKADSTRLMLHSKAQTTVLHLAAERGAVEDLELHEVMLTGFRGVKCVESGGPE 96 Query: 58 XXXXXXXXXIITSVSLLKQQRIFEELDLDFVIYDVLGDVVCGGFAVPVREGIAEHVYTVT 117 IIT+++ L++ ++ DLDFV YDVLGDVVCGGFA+P+REG A+ +Y VT Sbjct: 97 PGVGCAGRGIITAINFLEENGAYQ--DLDFVSYDVLGDVVCGGFAMPIREGKAQEIYIVT 154 Query: 118 SADFMAIYAANNLFKGIQKYSQDGGALLGGVIANSINAPYAKDIVDDFVKRTDTQVVEYV 177 S + MA+YAANN+ +GI KY+ GG LGG+I NS +++ + +R +TQ++ +V Sbjct: 155 SGEMMAMYAANNIARGILKYAHSGGVRLGGLICNSRKVDREDELIMNLAERLNTQMIHFV 214 Query: 178 PRSVTVTQSELQGKTTIEAAPDSEQAKVYRNLARKIADHTVSKVPAPLDTQELRQWAAEW 237 PR V +EL+ T E APDS Q + YR LA+KI ++ +P P++ EL E+ Sbjct: 215 PRDNIVQHAELRRMTVNEYAPDSNQGQEYRALAKKIINNDKLTIPTPMEMDELEALLIEY 274 Query: 238 G 238 G Sbjct: 275 G 275 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,814,565 Number of extensions: 588633 Number of successful extensions: 1358 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 1333 Number of HSP's successfully gapped: 13 Length of query: 257 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 153 Effective length of database: 26,383,695 Effective search space: 4036705335 Effective search space used: 4036705335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)