BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0023.p (162 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1517 [R] COG0535 Predicted Fe-S oxidoreductases 82 5e-16 SMa0814 [R] COG0535 Predicted Fe-S oxidoreductases 72 3e-13 MA1221 [R] COG0535 Predicted Fe-S oxidoreductases 65 4e-11 MA2936 [R] COG0535 Predicted Fe-S oxidoreductases 63 2e-10 CAC0259_2 [R] COG0535 Predicted Fe-S oxidoreductases 59 5e-09 >all1517 [R] COG0535 Predicted Fe-S oxidoreductases Length = 346 Score = 81.6 bits (200), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 0/149 (0%), Positives = 0/149 (0%), Gaps = 11/149 (7%) Query: 5 PVYTLEERENE---LQKLDNELQVRRDRRQNGTAPGSGG--IRIAVASRGGGKVNQHFGH 59 + Sbjct: 326 LSEDVLAEIEAVHQ 385 Query: 60 AREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGILKETVEMLRDCRLLLCAGI 119 Sbjct: 386 ------ 439 Query: 120 GPVPQEVLHRAEITVIVTKDAIESSLVKY 148 Sbjct: 440 468 >SMa0814 [R] COG0535 Predicted Fe-S oxidoreductases Length = 346 Score = 72.4 bits (176), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 0/109 (0%), Positives = 0/109 (0%), Gaps = 8/109 (7%) Query: 39 GGIRIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGA 98 Sbjct: 369 ------ 422 Query: 99 GILKETVEMLRDCRLLLCAGIGPVPQEVLHRAEI--TVIVTKDAIESSL 145 Sbjct: 423 471 >MA1221 [R] COG0535 Predicted Fe-S oxidoreductases Length = 346 Score = 65.1 bits (157), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 8/121 (6%), Positives = 18/121 (14%), Gaps = 16/121 (13%) Query: 38 SGGIRIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQG 97 + +A G + K R+ Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH---------D 116 Query: 98 AGI------LKETVEMLRDCRLLLCAGIGPVPQEVLHRAEITVIVTKDAIESSLVKYGRL 151 + L +G + + + A R Sbjct: 117 SLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRA-GKIRY 175 Query: 152 L 152 + Sbjct: 176 I 176 >MA2936 [R] COG0535 Predicted Fe-S oxidoreductases Length = 346 Score = 62.8 bits (151), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 2/106 (1%), Positives = 15/106 (14%), Gaps = 4/106 (3%) Query: 40 GIRIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAG 99 I + + + + + + Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVA-EMYPVPPRPE--- 60 Query: 100 ILKETVEMLRDCRLLLCAGIGPVPQEVLHRAEITVIVTKDAIESSL 145 T + + + + + ++ Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALD 106 >CAC0259_2 [R] COG0535 Predicted Fe-S oxidoreductases Length = 346 Score = 58.5 bits (140), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 0/104 (0%), Positives = 0/104 (0%), Gaps = 3/104 (2%) Query: 42 RIAVASRGGGKVNQHFGHAREFLVYEVKAESARLLGVRRIQAYCNGTAACAGEEQGAGIL 101 Sbjct: 297 -- 354 Query: 102 KETVEMLRDCRLLLCAGIGPVPQEVLHRAEITVIVTKDAIESSL 145 Sbjct: 355 - 397 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,640,952 Number of extensions: 462749 Number of successful extensions: 750 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 746 Number of HSP's successfully gapped: 5 Length of query: 162 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 64 Effective length of database: 27,075,699 Effective search space: 1732844736 Effective search space used: 1732844736 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)