BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0022.p (342 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0882 [J] COG2265 SAM-dependent methyltransferases related to ... 301 1e-81 CAC2783 [E] COG2873 O-acetylhomoserine sulfhydrylase 244 1e-64 RSc1562 [E] COG2873 O-acetylhomoserine sulfhydrylase 232 6e-61 DR0873 [E] COG2873 O-acetylhomoserine sulfhydrylase 190 3e-48 CAC0102 [E] COG2873 O-acetylhomoserine sulfhydrylase 184 1e-46 >STM0882 [J] COG2265 SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase Length = 376 Score = 301 bits (771), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 17/334 (5%), Positives = 46/334 (13%), Gaps = 10/334 (2%) Query: 3 MTDKKLGFDTLKVRAGYDSREHNY-AVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTR 61 M + + + L + + G E+ + Sbjct: 1 MQCALYDAGRCRSCQWITQSVNEQLSAKTADLHRLLAGLPVEQWCAPIGGPEQHFRNKAK 60 Query: 62 IGNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDAL 121 + ++ + A Y Sbjct: 61 MVVSGSVEKPLFGMLHRDGTPVDLCGCPLYPASFDPVFSALKPFIARAGLTPYNVARKRG 120 Query: 122 KH-LYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDN 180 + D +A L + + I Sbjct: 121 ELKYLLLTESQFDGG-MMLRFVLRSETKLTQLRAALPWLRAQLPQLRVITANIQPVHMAI 179 Query: 181 GIPLVIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPH 240 + + I + R Sbjct: 180 MEGETEIYLTD-QQALAERFNDVPLWIRPQSFFQTNPTVASRLYATARDWVGQLPVRHMW 238 Query: 241 FEEPQYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLS 300 G + L Q +++ Sbjct: 239 DLFCG------VGGFGLHCATPQMQLTGIEIAPEAIACAKQSAAELGLTRLHFQALDSTQ 292 Query: 301 ERISKQVANALRIVDYLQKHDQVSWVSHPVAEGN 334 ++ L +V+ ++ + Sbjct: 293 FATAQGETPDLVLVNPPRRGIGKPLCDYLAQMAP 326 >CAC2783 [E] COG2873 O-acetylhomoserine sulfhydrylase Length = 427 Score = 244 bits (623), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 149/335 (44%), Positives = 204/335 (60%), Gaps = 15/335 (4%) Query: 5 DKKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGN 64 ++K GF+TL+V AG + + AVPIYQT SY + + LF ++EPG +YTRI N Sbjct: 4 ERKFGFETLQVHAGQVADPTTGSRAVPIYQTTSYVFKNADHAANLFQLKEPGNVYTRIMN 63 Query: 65 PTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHL 124 PT V EQR+ AL+ TYA+LN+A G I+ LYGGT++ Sbjct: 64 PTTDVFEQRVAALEGGVAGLATASGLAAITYAILNVASAGDEIVAASTLYGGTYELFGVT 123 Query: 125 YPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPL 184 K G+ V FV D D+PE R+ I TKA+ E+I NP VLDIEA+A+ AH+N IPL Sbjct: 124 LKKLGIKVVFV-DPDNPENIRKAINDRTKAVYGETIGNPRINVLDIEAVAKIAHENKIPL 182 Query: 185 VIDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNW-ANGRFPHFEE 243 +IDNTFGTPYL GADIV++SATK IGGHGTT+GG+I++ G F+W A+G+FP F Sbjct: 183 IIDNTFGTPYLIRPIEFGADIVVHSATKFIGGHGTTIGGIIVDGGKFDWRASGKFPDFTT 242 Query: 244 PQYLLREAETGRERSILEVFPD--AP-FTARVRLNYLAYFGAVLGPFDAFLLLQGIETLS 300 P ++ G ++ D AP F + R+ L GA L P AF LQG+E+LS Sbjct: 243 PD----KSYNGL------IYADLGAPAFALKARVQLLRNTGATLSPQSAFYFLQGLESLS 292 Query: 301 ERISKQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 R+ K V N ++V++L+ H +VSW+++P E +P Sbjct: 293 LRVQKHVDNTRKVVEFLKNHPKVSWINYPELEESP 327 >RSc1562 [E] COG2873 O-acetylhomoserine sulfhydrylase Length = 444 Score = 232 bits (592), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 128/331 (38%), Positives = 185/331 (55%), Gaps = 8/331 (2%) Query: 6 KKLGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNP 65 K FDTL + AG A A PI+ T S+ E LF ME G++Y+RI NP Sbjct: 2 SKARFDTLALHAGAAPDPATGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNP 61 Query: 66 TVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLY 125 TVAV E+R+ AL+ A++ L G I+ + LYGG+ + L + Sbjct: 62 TVAVFEERMAALENGVGAIATASGQAALHLAIVTLMGAGSHIVASSALYGGSHNLLHYTL 121 Query: 126 PKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLV 185 +FG+ FV+ D + +R I P+T+ + E++ NP VLDI +A AHD G+PL+ Sbjct: 122 RRFGIETTFVKPGDI-DGWRAAIRPETRLLFGETLGNPGLDVLDIPTVASIAHDAGVPLL 180 Query: 186 IDNTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNW-ANGRFPHFEEP 244 +D+TF TP+L FAHGAD++ +SATK +GGHGTT+GGV+++ G F++ A GR P EP Sbjct: 181 VDSTFTTPWLLQPFAHGADLLYHSATKFLGGHGTTIGGVLVDAGTFDYVAAGRHPELTEP 240 Query: 245 QYLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERIS 304 + E ++ APF R R L FGA + P A+ LLQG+ETL R++ Sbjct: 241 YAGFHDMVFAEESTV------APFLLRARREGLRDFGACMNPMAAWQLLQGVETLPLRMA 294 Query: 305 KQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 + V N R+V +L H V+ V++P E +P Sbjct: 295 RHVENTRRVVAFLAGHPMVASVAYPELETHP 325 >DR0873 [E] COG2873 O-acetylhomoserine sulfhydrylase Length = 573 Score = 190 bits (483), Expect = 3e-48, Method: Compositional matrix adjust. Identities = 130/341 (38%), Positives = 182/341 (53%), Gaps = 9/341 (2%) Query: 3 MTDKK----LGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYL 58 MTD K L F+TL+V AG A PIY T SY S E LFG+ + G + Sbjct: 130 MTDTKQPQPLHFETLQVHAGQRPDPVTGAQQTPIYATNSYVFESPEHAADLFGLRQFGNI 189 Query: 59 YTRIGNPTVAVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTF 118 Y+RI NPT V EQR+ AL+ LL LA+ G I+++P LYGGT Sbjct: 190 YSRIMNPTNDVFEQRVAALEGGVGALSVSSGHAGQLVTLLTLAQAGDNIVSSPNLYGGTV 249 Query: 119 DALKHLYPKFGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAH 178 + + + G+ V F + PE F I T+A+ +E+I NP + D E +A+ AH Sbjct: 250 NQFRVTLKRLGIEVRFTSKDERPEEFAALIDERTRAVYLETIGNPALNIPDFEGVAKVAH 309 Query: 179 DNGIPLVIDNTFGT-PYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGR 237 ++G+ +V+DNTFG Y HGA+IV++SA+K IGGHG +GGVI++ GNF+W +GR Sbjct: 310 EHGVAVVVDNTFGAGGYYCQPLRHGANIVLHSASKWIGGHGNGIGGVIVDGGNFDWGSGR 369 Query: 238 FPHFEEPQ---YLLREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQ 294 +P EP + L+ ET E + L + P+ F R R L G L P A+ LQ Sbjct: 370 YPLMTEPSPSYHGLKFWETFGEGNGLGL-PNIAFITRARTEGLRDLGTTLAPQQAWQFLQ 428 Query: 295 GIETLSERISKQVANALRIVDYLQKHDQVSWVSHPVAEGNP 335 G+ETLS R + N L + +L H V V++P +P Sbjct: 429 GLETLSLRAERHAENTLALAHWLISHPDVKQVTYPGLSNHP 469 >CAC0102 [E] COG2873 O-acetylhomoserine sulfhydrylase Length = 409 Score = 184 bits (468), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 104/322 (32%), Positives = 170/322 (52%), Gaps = 18/322 (5%) Query: 8 LGFDTLKVRAGYDSREHNYAVAVPIYQTASYDLGSVERGEKLFGMEEPGYLYTRIGNPTV 67 + F T + Y+ E+ A VPIY + +Y S + E +F + PGY+Y+R NPTV Sbjct: 1 MKFSTKLIHGNYN-LENTGATNVPIYMSNAYAHSSPQELENVFKGKFPGYVYSRFSNPTV 59 Query: 68 AVLEQRLTALDKXXXXXXXXXXXXXXTYALLNLAEGGGRILTTPRLYGGTFDALKHLYPK 127 E+R+ +++ A+ N+ G I+ + LYGGT+ + +L Sbjct: 60 LEFERRMASIEGGLTATSAASGMSAIYMAITNIVHPGDEIIASTGLYGGTYTLISNL-QN 118 Query: 128 FGVHVDFVEDSDDPEAFRRDIGPDTKAILVESISNPNSTVLDIEAIAEAAHDNGIPLVID 187 GV V F+ED + +I +TK + E+I NP +LDIE++ + GI ++D Sbjct: 119 IGVKVIFLEDIS-KKTLLNNINENTKLVYTETIGNPKLDILDIESVGRVCKEKGIIFMVD 177 Query: 188 NTFGTPYLFDSFAHGADIVIYSATKAIGGHGTTLGGVILENGNFNWANGRFPHFEEPQYL 247 +T TPYL + +GAD++I+S +K I G +GG+I++ G+ + N R+ +F++ Sbjct: 178 STISTPYLINPSKYGADVIIHSTSKYINGTSNAIGGMIVDCGSEKYKNHRYENFQKYA-- 235 Query: 248 LREAETGRERSILEVFPDAPFTARVRLNYLAYFGAVLGPFDAFLLLQGIETLSERISKQV 307 E + FTA+++ GA + PF+AFL L GIETLS R+ K Sbjct: 236 -------------EKYDKLAFTAKLKSTSGLDIGASMSPFNAFLSLTGIETLSLRMKKHC 282 Query: 308 ANALRIVDYLQKHDQVSWVSHP 329 NA++I YL+K D+V+ V++P Sbjct: 283 ENAMKIASYLEKSDKVTNVNYP 304 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,750,263 Number of extensions: 1198231 Number of successful extensions: 2401 Number of sequences better than 1.0e-05: 98 Number of HSP's gapped: 2251 Number of HSP's successfully gapped: 104 Length of query: 342 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 235 Effective length of database: 26,037,693 Effective search space: 6118857855 Effective search space used: 6118857855 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)