BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0369.0018.p (493 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA1219 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha an... 124 5e-28 all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 112 2e-24 MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 108 2e-23 MTH1563 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 107 5e-23 mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha a... 106 1e-22 >MA1219 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 522 Score = 124 bits (310), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 112/460 (24%), Positives = 193/460 (41%), Gaps = 41/460 (8%) Query: 24 FAGTAAGLLSCSNNGGMKDGSRSFSQCMGCSSGNAFCQLSMITDAAMVNHAPVGCAGDFF 83 F G + L + +G + D S C A LS I +A ++ H P GCA F Sbjct: 24 FFGNLSELYQLAKDGKI-DTKLQGSHTRPCKFWTAMKILSGIKNAVVIAHGPSGCA---F 79 Query: 84 GYNFVYRVGQMERNLPPVIGRYFNTNIEEMDTVFGGARKLEATIKLAYERVKPNAIFVTT 143 G Y++ + P TNI+E V+GG R+L+ I+ + KP+ IFV T Sbjct: 80 GVKQTYKLTNCRNSGSPYEA-VITTNIDEKAVVYGGERELKGAIQEVDSKYKPDIIFVAT 138 Query: 144 SCASGIIGDDVESVTNKMTKELGIPVVTCFCEGFKSKIWTSGFDSAYHSIVRKIVKP-PE 202 SCA+GIIGD V++V +++ E+ ++ CEGF + + SGFD + IV+ + KP E Sbjct: 139 SCATGIIGDYVDAVVSRVRPEINAKIMAVHCEGFAGE-YRSGFDLVFRQIVQLMDKPDSE 197 Query: 203 KKTNKVNIINFWG-----------SDV--FSGLLNKLGYEADYIVPFS-TVAQLERISEA 248 + + +N G +DV L+ +G + I+ + ++ +++R Sbjct: 198 SRVALASSVNIVGGKMGPERTEIDTDVKELRRLIEDMGASVNAIIAGNCSLEEIQRAPSV 257 Query: 249 AATIQICPTLGTYLGAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLDRXXXXXXXXX 308 A +C LG +G + + P + YG++ T+ W+RE + L Sbjct: 258 AVNCTLCLDLGYAIGREMLDQFDTPLNSTILPYGISATERWLREAAKHLHMEAEAEALIK 317 Query: 309 XXXXXVLPQLEAYRAKLAGTTCYITAGSAHGHALIALLRELGINVQGAAIFHHDPVYDNG 368 + + E + LAG + A IA + E +++ H + Sbjct: 318 REYEAIRVEFEEAKKHLAGKLAIVEGHDAIKALSIAHMLERDFDMRVVIYNFHPWSTEAR 377 Query: 369 DPAADMLDHT------VKTYGDVP--NYNVCNKQAYELANILNRVCPDLMIARHGGMTHW 420 + + D L T + T G + Y + EL L + P+ + ++ Sbjct: 378 ETSIDYLLETGLDPEILITKGTLAFGKYEAMLQTENELLEYLGDMSPERTV-------YF 430 Query: 421 GAKLGIPTLLIGD-----EHFSWGYRGLLNYAERILETLD 455 G+ + P + + D +GYRG L A+ I L+ Sbjct: 431 GSSMSFPNIPVVDLNAILNRPRFGYRGALKVAKCICTALE 470 >all1438 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 480 Score = 112 bits (279), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 109/420 (25%), Positives = 173/420 (41%), Gaps = 40/420 (9%) Query: 52 GCSSGNAFCQLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIE 111 GC+ A L I DAA + H P+ CAG+ +G G P + F T++ Sbjct: 44 GCAFDGAMISLVPIVDAAHLVHGPIACAGNSWGSRGSLSSG------PQLYKMGFTTDMS 97 Query: 112 EMDTVFGGARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVVT 171 E D +FGG +KL I ++R P+A+FV +C + +IGDD+++V +++G PV+ Sbjct: 98 ENDVIFGGEKKLYKAILEIHKRYNPSAVFVYATCVTALIGDDIDAVCKTAAEKIGTPVIP 157 Query: 172 CFCEGF-KSKIWTSGFDSAYHSIVRKIVKPPEKKTNKVNIINFWGSDVFSG-------LL 223 GF SK + F S++ +V E + IN G +G LL Sbjct: 158 VIAPGFIGSKNLGNRFGG--ESLLDYVVGTAEPEYTTPYDINLIGEYNIAGEMWGVLPLL 215 Query: 224 NKLGYEADYIVPFSTVAQLERIS---EAAATIQICPTLGTYLGAALEQVYGVPEIKAPVA 280 KLG + + A+ E I A + IC + +E+ YG+P I+ Sbjct: 216 EKLGIRV--LSKITGDARFEEIRYAHRAKLNVMICSRALLNMARKMEENYGIPYIEESF- 272 Query: 281 YGVAGTDAWMRELGRVL---DRXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSA 337 YG+ + +R + L D + L YRA+L G + G Sbjct: 273 YGIDDMNRCLRNIAAKLGDPDLQARTEKLIAEETAALDLALAPYRARLKGKRVVLYTGGV 332 Query: 338 HGHALIALLRELGINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYE 397 ++I+ ++LGI V V + + + +K + A E Sbjct: 333 KSWSIISAAKDLGIEV----------VATSTRKSTEEDKAKIKRLLGADGIMLEKGNAKE 382 Query: 398 LANILNRVCPDLMIARHGGMTHWGA-KLGIPTLLIGDE--HFSWGYRGLLNYAERILETL 454 L ++ D++IA GG + A K IP L I E H GY G++ A + E L Sbjct: 383 LLQLVKDTQADMLIA--GGRNQYTALKARIPFLDINQERHHPYAGYVGMIEMARELYEAL 440 >MTH1565 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 108 bits (269), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 0/423 (0%), Positives = 0/423 (0%), Gaps = 46/423 (10%) Query: 53 CSSGNAFCQLSMITDAAMVNHAPVGCA-------GDFFGYNFVYRVGQMERNLPPVIGRY 105 + + + + + G + + Sbjct: 64 MDTPQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLT------------- 110 Query: 106 FNTNIEEMDTVFGGARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKEL 165 + G + KL R+ + + + + Sbjct: 111 VKRVKKGTLVDIGADKLALCREKLTVNRIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDT 170 Query: 166 GIPVVTCFCEGFKSKIWTSGFDSAY-----HSIVRKIVKPPEKKTNKVNIINFWGSDVF- 219 + + + +++ + ++ E ++ Sbjct: 171 RRNLAESLKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAILFGGPYKGLP-EIDA 229 Query: 220 -SGLLNKLGYE-ADYIVPFSTVAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIKA 277 + G Sbjct: 230 DIWVNTLPGQCT 289 Query: 278 PVAYGVAGTDAWMRELGRVLDRXXXXXXXXXXXXXXVLPQLEAYRAKLAGTTCYITAGSA 337 Sbjct: 290 -- 347 Query: 338 HGHALIALLRELGINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYNVCNKQAYE 397 Sbjct: 348 ---------- 397 Query: 398 LANILNRVCPDLMIARHGGMTHWGAKLGIPTLLIGDEH---FSWGYRGLLNYAERILETL 454 Sbjct: 398 -- 455 Query: 455 DNR 457 Sbjct: 456 458 >MTH1563 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 268 Score = 107 bits (266), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 0/438 (0%), Positives = 0/438 (0%), Gaps = 45/438 (10%) Query: 44 SRSFSQCM---GCSSGNAFCQLS-MITDAAMVNHAPVGCAGDFFGYNFVYRVGQM-ERNL 98 S + + + + ++T + V + + + Sbjct: 36 SIFGVKRIVIYHDDADGEARFIRDILTYMDTPQYLRRKVFPIMRELKHVGILPPLRTPHH 95 Query: 99 PPVIGRYFN------TNIEEMDTVFGGARKLE-ATIKLAYERVKPNAIFVTTSCASGIIG 151 P + ++ + + + V F I+ Sbjct: 96 PTG--KPVTGEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVNRIMSFRVVRLGKEILI 153 Query: 152 DDVESVTNKMTKELGIPVVTCFCEGFKSKIWTSGFDSAYHSIVRKIV---KPPEKKTNKV 208 + E E+ S S + I+ K + + Sbjct: 154 EPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPITSILDEVKTRMRGAREA 213 Query: 209 NIINFWGSDVFSGLLNKLGYEADYIVPFSTVA------QLERISEAAATIQICPTLGTYL 262 I+ + + + + Sbjct: 214 AILFGGPYKGLPEIDADI-WVNTLPGQCTETVRTEEAV 272 Query: 263 GAALEQVYGVPEIKAPVAYGVAGTDAWMRELGRVLDRXXXXXXXXXXXXXXVLPQLEAYR 322 Sbjct: 273 - 331 Query: 323 AKLAGTTCYI-TAGSAHGHALIALLRELGINVQGAA-IFHHDPVYDNGDPAADMLDHTVK 380 Sbjct: 332 -- 389 Query: 381 TYGDVPNYNVCNKQAYELANILNRVCPDLMIA--RHGGMTHWGAKLGIPTLLIGD-EHFS 437 Sbjct: 390 ------------ 437 Query: 438 W-GYRGLLNYAERILETL 454 Sbjct: 438 455 >mlr5908 [C] COG2710 Nitrogenase molybdenum-iron protein, alpha and beta chains Length = 496 Score = 106 bits (264), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 106/424 (25%), Positives = 176/424 (41%), Gaps = 51/424 (12%) Query: 52 GCSSGNAFCQLSMITDAAMVNHAPVGCAGDFFGYNFVYRVGQMERNLPPVIGRYFNTNIE 111 GC+ A L ITD A + HAP+ C G+ + G P + F T++ Sbjct: 44 GCAFDGAKIVLQPITDVAHLVHAPLACEGNSWDNRGAASSG------PTLWRTSFTTDLT 97 Query: 112 EMDTVFG-GARKLEATIKLAYERVKPNAIFVTTSCASGIIGDDVESVTNKMTKELGIPVV 170 E+D V G G RKL I+ E P A+FV ++C + +IGDD+E+V + ++ G+ VV Sbjct: 98 ELDIVMGQGERKLFKAIREIKETYAPPAVFVYSTCVTALIGDDIEAVCKRAAEKFGLAVV 157 Query: 171 TCFCEGFKSKIWTSGFDSAYHSIVRKI---VKPPEKKTNKVNII-------NFWGSDVFS 220 G G A +++ + V+P + +NI+ FW + Sbjct: 158 PINAPGLAGSK-NLGNKLAAEALLDHVIGTVEPDDAGPYDINILGEFNLSGEFW---LVK 213 Query: 221 GLLNKLGYEADYIVP----FSTVAQLERISEAAATIQICPTLGTYLGAALEQVYGVPEIK 276 LL++LG +P + VA R A A + +C + L +E+ + +P + Sbjct: 214 PLLDRLGIRVRACIPGDARYIDVASAHR---ARAAMMVCSSALINLARKMEERWDIPYFE 270 Query: 277 APVAYGVAGTDAWMRELGRVLDRXXXXXXXXXXXXXXVLPQ-------LEAYRAKLAGTT 329 YG+ T +R + +L R + Q LEAYR +L G Sbjct: 271 GSF-YGITDTSEALRNIAELLVRKGADAEILDRTETLIAEQEAIAWKKLEAYRRRLQGKR 329 Query: 330 CYITAGSAHGHALIALLRELGINVQGAAIFHHDPVYDNGDPAADMLDHTVKTYGDVPNYN 389 + G +++ L E+G+ + G ++ V D +K N+ Sbjct: 330 VLLNTGGVKSWSVVHALMEIGMEIVGTSV-KKSTVEDK---------ERIKRILKDENHM 379 Query: 390 VCNKQAYELANILNRVCPDLMIARHGGMTHWGA-KLGIPTLLIGDE--HFSWGYRGLLNY 446 A +L +L++ D+M++ GG T + A K P L I E H GY G++ Sbjct: 380 FEQMAARDLYAMLSKHKADIMLS--GGRTQFVALKAKTPWLDINQERQHPYAGYDGMVEL 437 Query: 447 AERI 450 +I Sbjct: 438 VRQI 441 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,600,598 Number of extensions: 1580487 Number of successful extensions: 2733 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 2701 Number of HSP's successfully gapped: 19 Length of query: 493 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 383 Effective length of database: 25,691,691 Effective search space: 9839917653 Effective search space used: 9839917653 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)