BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0033.p (458 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy1096 [H] COG0285 Folylpolyglutamate synthase 224 2e-58 SP0290 [H] COG0285 Folylpolyglutamate synthase 217 3e-56 SPy0814 [H] COG0285 Folylpolyglutamate synthase 199 1e-50 SP0197 [H] COG0285 Folylpolyglutamate synthase 194 4e-49 YMR113w [H] COG0285 Folylpolyglutamate synthase 172 1e-42 >SPy1096 [H] COG0285 Folylpolyglutamate synthase Length = 252 Score = 224 bits (572), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 0/436 (0%), Positives = 0/436 (0%), Gaps = 30/436 (6%) Query: 15 SYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTSVL 74 S +++ +E + + + A + + Sbjct: 2 SDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGI 61 Query: 75 IRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQ-TELGSPTMF 133 S + S + + AN + + + + Sbjct: 62 DSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLN 121 Query: 134 EVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLEA 193 + + V++ E D Sbjct: 122 PQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRT 181 Query: 194 IAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQTF 253 + + + VT + Y +G Sbjct: 182 TYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQ-ENG----Q 236 Query: 254 TFKGPFRSL-ELGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAGR 312 R Sbjct: 237 ILLAFPRLFI- 295 Query: 313 LEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPIV 372 Sbjct: 296 ------------ 343 Query: 373 DTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEH--DWGQALQLLQSITAEQD 430 Sbjct: 344 -------- 395 Query: 431 LAVVSGTLYLISDVRS 446 Sbjct: 396 411 >SP0290 [H] COG0285 Folylpolyglutamate synthase Length = 252 Score = 217 bits (553), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 0/430 (0%), Positives = 0/430 (0%), Gaps = 18/430 (4%) Query: 34 GLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTSVLIRSGYSVGTFTSPYITKFT 93 +E + + + A + + S + S Sbjct: 21 VELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSALPDCQF 80 Query: 94 NRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPTMFEVATALAILYYAEVCYPDVV 153 + + AN + + + ++ LA+ Sbjct: 81 VEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNW-LEPTH 139 Query: 154 VWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLEAIAGEKAGIIKPGVPAVTCVS 213 V + + + ++ + ++ + + + Sbjct: 140 VLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTA 199 Query: 214 QPEXXXXXXXXXXXXXXXXXXXGEDFS--YGSAVVSGGLQTFTFKGPFRSLELGIAMKGE 271 + Y Sbjct: 200 TGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQ-- 257 Query: 272 HQLSNSAAAMMALEVLRQYMAFILEDEDVL-EGFRSTFWAGRLEEVSTSPRIVLDGAHNP 330 Sbjct: 258 317 Query: 331 EGAESLAKSLPQ-FYKYDKLNLLMGMLANKHHESYFKHILPIVDTLILTEPDFRKKMDAE 389 Sbjct: 318 ------ 371 Query: 390 DLQAIAER-LREKYAKENLVIKVEHDWGQALQLLQSITAEQDLAVVSGTLYLISDVRSTL 448 Sbjct: 372 --- 428 Query: 449 LRQPNSEKGW 458 Sbjct: 429 - 437 >SPy0814 [H] COG0285 Folylpolyglutamate synthase Length = 252 Score = 199 bits (505), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 0/443 (0%), Positives = 0/443 (0%), Gaps = 30/443 (6%) Query: 13 LTSYTEAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTS 72 ++ + +++ +E + + + A + Sbjct: 1 MSDW-NPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARIT 59 Query: 73 VLIRSGYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMA-QTELGSPT 131 + S + S + + AN + + Sbjct: 60 GIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSL 119 Query: 132 MFEVATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTL 191 + + + V++ E D Sbjct: 120 LNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIW 179 Query: 192 EAIAGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQ 251 + + + VT + Sbjct: 180 RTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEE-----QYPLQEN 234 Query: 252 TFTFKGPFRSL-ELGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWA 310 R Sbjct: 235 GQILLAFPRLFI- 293 Query: 311 GRLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILP 370 Sbjct: 294 ------------ 341 Query: 371 IVDTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEHDWGQALQLLQSITAEQD 430 Sbjct: 342 --------- 392 Query: 431 LAVVSGTLYLISDVRSTLLRQPN 453 Sbjct: 393 415 >SP0197 [H] COG0285 Folylpolyglutamate synthase Length = 252 Score = 194 bits (492), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 0/444 (0%), Positives = 0/444 (0%), Gaps = 48/444 (10%) Query: 18 EAVDWINGLIPFGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTSVLIRS 77 + + + + + + + Sbjct: 3 DWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGID 62 Query: 78 GYSVGTFTSPYITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSP---TMFE 134 + + + + +L + + Sbjct: 63 SSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFA----NASLQWLPDHYELFPHLVS 118 Query: 135 VATALAILYYAEVCYPDVVVWETGLGGRLDVTNIVTPIVSVITNVGHDHTDVLGDTLEAI 194 + +L + + N + V + + E Sbjct: 119 LLNPQGVLAVQM-PDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVD 177 Query: 195 AGEKAGIIKPGVPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQTFT 254 + Sbjct: 178 IWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYH-------------QM 224 Query: 255 FKGPF-----RSLELGIAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFW 309 + + Sbjct: 225 LEEQYPLQEN 284 Query: 310 AGRLEEVSTSPRIVLDGAHNPEGAESLAKSLPQFYKYD-KLNLLMGMLANKHHE---SYF 365 Sbjct: 285 -- 342 Query: 366 KHILPIVDTLILTEPDFRKKMDAEDLQAIAERLREKYAKENLVIKVEHDWGQALQLLQSI 425 Sbjct: 343 ---------------- 386 Query: 426 TAEQDLAVVSGTLYLISDVRSTLL 449 Sbjct: 387 410 >YMR113w [H] COG0285 Folylpolyglutamate synthase Length = 427 Score = 172 bits (435), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 143/443 (32%), Positives = 206/443 (46%), Gaps = 37/443 (8%) Query: 29 FGIRPGLERIERLMEKLGQPHRRLKFIHVAGTNGKGSTCAFLTSVLIRSGYSVGTFTSPY 88 I GL RI +L+E LG P L+ +H+AGTNGKGS C +L+SVL + Y +G FT+P+ Sbjct: 1 MAIELGLSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSSVLQQKSYQIGKFTTPH 60 Query: 89 ITKFTNRFQYNGVDIPENVLTELANRLHPLVEEMAQTELGSPTMFEVATALAILYYAEVC 148 + T+ N IP E + +E + ++ T FE+ T A Y+ +V Sbjct: 61 LVHVTDSITINNKPIP----LERYQNIRLQLEALNKSHSLKCTEFELLTCTAFKYFYDVQ 116 Query: 149 YPDVVVWETGLGGRLDVTNIV---TPIVSVITNVGHDHTDVLGDTLEAIAGEKAGIIKPG 205 V+ E GLGGRLD TN++ IT + DH LG+TL I+ EKAGII G Sbjct: 117 CQWCVI-EVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNTLSEISKEKAGIITEG 175 Query: 206 VPAVTCVSQPEXXXXXXXXXXXXXXXXXXXGEDFSYGSAVVSGGLQTFTFKGPFRSLELG 265 VP E G + S + ++G + G F +L Sbjct: 176 VPFTVIDGTNE-----ASVINVVKERCKALGSELSVTDSQLNGNMIDTNSWGCFDLAKL- 229 Query: 266 IAMKGEHQLSNSAAAMMALEVLRQYMAFILEDEDVLEGFRSTFWAGRL-------EEVS- 317 + GE+Q+ N AM L+ L+ + +V W GRL ++VS Sbjct: 230 -PLNGEYQIFNLRVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRLYRMDYRFDKVSN 288 Query: 318 -TSPRIVLDGAHNPEGAESLAKSLPQFYKYDKLNLLMGMLANKHHESYFKHILPIVDTLI 376 T P I++DGAHN A L K L + Y L +M + K+ E + +L +D +I Sbjct: 289 RTVP-ILMDGAHNGSAAVELVKYLRKEYGNQPLTFVMAVTHGKNLEPLLQPLLRPIDQVI 347 Query: 377 LTEPDFRKKM---DAEDLQAIAER-LREKYAKENLVIKVEHDWGQALQLLQSITAEQDLA 432 LT + + M A D + I + L + Y KE I +E+D Q L L ++ EQ Sbjct: 348 LTRFNNVEGMPWIHATDPEEIKDFILTQGYTKE---IVIENDLHQVLPSLAHVSDEQRRP 404 Query: 433 -VVSGTLYLISDVRSTLLRQPNS 454 VV G+LYL + LLR NS Sbjct: 405 IVVCGSLYLCGE----LLRIHNS 423 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,076,468 Number of extensions: 1329657 Number of successful extensions: 4414 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 4334 Number of HSP's successfully gapped: 28 Length of query: 458 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 348 Effective length of database: 25,691,691 Effective search space: 8940708468 Effective search space used: 8940708468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)