BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0018.p (427 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4477 [J] COG1530 Ribonucleases G and E 310 3e-84 VC0419 [J] COG1530 Ribonucleases G and E 297 2e-80 XF1125 [J] COG1530 Ribonucleases G and E 288 1e-77 PM0342 [J] COG1530 Ribonucleases G and E 287 2e-77 STM3370 [J] COG1530 Ribonucleases G and E 284 2e-76 >PA4477 [J] COG1530 Ribonucleases G and E Length = 485 Score = 310 bits (794), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 163/411 (39%), Positives = 251/411 (61%), Gaps = 11/411 (2%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 ++++++ +R+A+++NG L E ER Q++G+VG+ YKGRV+ VLPGMQAAFVDIG Sbjct: 3 EEILINITPMESRVAVVENGVLQEVHVERTQRRGIVGNIYKGRVVRVLPGMQAAFVDIGL 62 Query: 76 KKNAFLYVDDVLHPHLERQPEVKPSIETLLQPGQEIVVQVRKEPRGGKGARVTTHYTLPG 135 ++ AF++ ++ + R+ SI L+ GQ +VVQV K+P G KGAR+TTH ++P Sbjct: 63 ERAAFIHAAEISN----REGSAVESISALVHEGQALVVQVTKDPIGTKGARLTTHLSIPS 118 Query: 136 RWMVYMPFADYVGVSKKISRESERARLKAI------GERLRRQEEGIIMRTVSEDEPVEA 189 R++VYMP +VG+S +I E ER RLK + E + Q G I+RT +E + Sbjct: 119 RYLVYMPRTSHVGISLRIEDEVERERLKKVVADCVAAEGIEGQG-GFILRTAAEGAGEDE 177 Query: 190 VEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVRE 249 + D+ +LR W+ I + Q GAP++++ DLS+ R +RD NP+ +++ IDS + ++ Sbjct: 178 ILADIRYLRRLWDQIAAQIQTVGAPSVIYEDLSLAIRTLRDLVNPRIEKIRIDSRENFQK 237 Query: 250 AEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALT 309 +F+ ++ P + Y G+ IF YGV++++ K+ RK+ L+ G LI D TEA+T Sbjct: 238 ITSFVEELMPEISDRLEHYPGERPIFDLYGVEDEIQKALERKVLLKSGGYLIVDPTEAMT 297 Query: 310 VIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVT 369 IDVNT + G +LEET+ +TNL AA I R +RLR+ M+ EEHR+ V Sbjct: 298 TIDVNTGAFVGHRNLEETIFKTNLEAATAIARQLRLRNLGGIIIIDFIDMEDEEHRRQVL 357 Query: 370 ERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTG 420 LE+ + RD +T+I+G T LGL++MTRK+ R C CC G G Sbjct: 358 RTLEKQLERDHAKTNIIGITELGLVQMTRKRTRESLVQILCEPCPCCQGRG 408 >VC0419 [J] COG1530 Ribonucleases G and E Length = 489 Score = 297 bits (760), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 160/413 (38%), Positives = 243/413 (58%), Gaps = 9/413 (2%) Query: 17 QMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQK 76 +++++ TR+A+++ G L E ER+ ++G+VG+ YKG+V VLPGMQAAFVDIG Sbjct: 4 ELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDIGLD 63 Query: 77 KNAFLYVDDVLHPHLE-------RQPEVKPSIETLLQPGQEIVVQVRKEPRGGKGARVTT 129 K AFL+ D++ PH E +Q +V+ I L++ GQ+IVVQV K+P G KGAR+TT Sbjct: 64 KAAFLHASDIV-PHTECVAESEKQQFQVR-DISELVRQGQDIVVQVVKDPLGTKGARLTT 121 Query: 130 HYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSEDEPVEA 189 TLP R++V+MP A +VGVS++I E+ER RLK I + G I+RT +E + Sbjct: 122 DITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSHYCDEHGGFIIRTAAEGANEKE 181 Query: 190 VEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVRE 249 + D +FL+ W + R + ++L+ +L + QR +RD + +++IDS + Sbjct: 182 LTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFEN 241 Query: 250 AEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALT 309 + F ++ P + YHG + IF Y + ++ +S RK+ L+ G LI D+TEA+T Sbjct: 242 LKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAMT 301 Query: 310 VIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVT 369 ID+NT + G +LEET+ TN+ A + I R +RLR+ M +EHRK V Sbjct: 302 TIDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRVL 361 Query: 370 ERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKV 422 LE + +DR +T+I G+T LGL+EMTRK+ R + C C G G V Sbjct: 362 TSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCSTCPTCEGRGSV 414 >XF1125 [J] COG1530 Ribonucleases G and E Length = 497 Score = 288 bits (736), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 162/425 (38%), Positives = 248/425 (58%), Gaps = 23/425 (5%) Query: 16 KQMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQ 75 ++++V+ TR+A+++NG L E ERD +G+VG+ YKGRV V+PGMQAAFVD+G Sbjct: 3 EEILVNVTFGETRVAVVENGLLQELHIERDGCRGVVGNIYKGRVQRVMPGMQAAFVDVGM 62 Query: 76 KKNAFLYVDDVLH----------------PHLERQPEVKPSIETLLQPGQEIVVQVRKEP 119 ++ AFL+V+DV+ P L P V+ LL+ GQE++VQV KEP Sbjct: 63 ERTAFLHVNDVVRSAPADSSVIDTDETTVPPLSEIPIVE-----LLRDGQELMVQVLKEP 117 Query: 120 RGGKGARVTTHYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQ--EEGII 177 G KGAR+TT ++P R++V +P + VGVS +I E+ERARLKA+ L Q + G I Sbjct: 118 IGTKGARLTTQISIPSRYLVLLPQSKMVGVSLRIEDEAERARLKAVVTDLAAQYGDYGYI 177 Query: 178 MRTVSEDEPVEAVEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQD 237 +RT +E + + + D+++L W ++ RR +E +LL+ DLS+ R +RD + Sbjct: 178 VRTNAEGQSADVLVEDIAYLSRLWGVVERRGRELPVCSLLYEDLSLSLRSVRDLIRKDVE 237 Query: 238 ELMIDSAKAVREAEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGG 297 ++ +DS +A + + F+ P + Y G IF YGV++++ ++ R+I L+ G Sbjct: 238 KIKVDSKEAFAQLQGFVTKYMPVLADKLELYVGDHPIFDLYGVEDEIGRALDRQIPLKSG 297 Query: 298 ATLIWDETEALTVIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXX 357 L+ ++TEA+T +DVNT + G +LEETV RTNL AA+ + R +RLR+ Sbjct: 298 GYLVIEQTEAMTTVDVNTGSFVGKRTLEETVFRTNLEAAQALARQLRLRNLGGIIVIDFI 357 Query: 358 XMQREEHRKMVTERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCG 417 MQ EHR V LE+ + RD +T + ++ LGL+EMTRK+ CQ CG Sbjct: 358 DMQDAEHRCQVLRVLEKALIRDHAKTTLYDFSPLGLVEMTRKRTVESLQRQLSETCQECG 417 Query: 418 GTGKV 422 G G + Sbjct: 418 GRGTI 422 >PM0342 [J] COG1530 Ribonucleases G and E Length = 491 Score = 287 bits (735), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 154/411 (37%), Positives = 238/411 (57%), Gaps = 5/411 (1%) Query: 17 QMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQK 76 +++V+ TR+AL+D G L E ER ++G+VG+ YKGRV VLPGMQ+AFVDIG + Sbjct: 5 ELLVNVTPSETRIALVDMGILKEVHIERQAKRGIVGNIYKGRVTRVLPGMQSAFVDIGLE 64 Query: 77 KNAFLYVDDVL-HPHLERQPE----VKPSIETLLQPGQEIVVQVRKEPRGGKGARVTTHY 131 K AFL+ D++ H + E V I L++ GQ+IVVQV K+P G KGAR+TT Sbjct: 65 KAAFLHASDIVSHTECVEESEQKQFVVKDIAELVREGQDIVVQVVKDPLGTKGARLTTDI 124 Query: 132 TLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSEDEPVEAVE 191 TLP R++V+MP +VGVS++I E ERARLK + L + G I+RT ++ E ++ Sbjct: 125 TLPSRYLVFMPENSHVGVSQRIESEEERARLKELVLPLCDELGGFIIRTAADGATEEELK 184 Query: 192 GDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVREAE 251 D FL+ W + R + ++L+ +L++ QR +RD +++ IDS E + Sbjct: 185 QDADFLKRLWRKVLERRAKYPTKSMLYGELALTQRILRDFIGAGLEKIRIDSKLCFTEVK 244 Query: 252 AFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALTVI 311 F + P + Y G + +F YGV+ + + +++ L+ G LI ++TEA+T I Sbjct: 245 QFTEEFMPTLVDKLVLYSGNQPLFDVYGVEAAIQTALNKRVNLKSGGYLIIEQTEAMTTI 304 Query: 312 DVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVTER 371 D+NT + G +LEET+ TN+ A + I + ++LR+ MQ ++HR V E Sbjct: 305 DINTGAFVGHRNLEETIFNTNIEATKAIAQQLQLRNLGGIIIIDFIDMQTDDHRVRVLES 364 Query: 372 LERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKV 422 L+ +S+DR +T + G+T LGL+EMTRK+ R C C G G++ Sbjct: 365 LQEALSKDRVKTSVNGFTQLGLVEMTRKRTRESLEHVLCGDCPACEGRGRI 415 >STM3370 [J] COG1530 Ribonucleases G and E Length = 489 Score = 284 bits (726), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 156/413 (37%), Positives = 238/413 (57%), Gaps = 9/413 (2%) Query: 17 QMIVHCMQHVTRMALLDNGSLVEYAAERDQQQGLVGSYYKGRVMNVLPGMQAAFVDIGQK 76 +++V+ TR+A +D G L E ER+ ++G+VG+ YKGRV VLPGMQAAFVDIG Sbjct: 4 ELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKGRVSRVLPGMQAAFVDIGLD 63 Query: 77 KNAFLYVDDVLHPHLE-------RQPEVKPSIETLLQPGQEIVVQVRKEPRGGKGARVTT 129 K AFL+ D++ PH E +Q V+ I L++ GQ+++VQV K+P G KGAR+TT Sbjct: 64 KAAFLHASDIM-PHTECVAGDEQKQFTVR-DISELVRQGQDLMVQVVKDPLGTKGARLTT 121 Query: 130 HYTLPGRWMVYMPFADYVGVSKKISRESERARLKAIGERLRRQEEGIIMRTVSEDEPVEA 189 TLP R++V+MP A +VGVS++I ESER RLK + ++ G I+RT +E E Sbjct: 122 DITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAEYCDEQGGFIIRTAAEGVCEED 181 Query: 190 VEGDLSFLRSQWEMITRRAQECGAPALLHSDLSIVQRFIRDAFNPQQDELMIDSAKAVRE 249 + D ++L+ W + R + ++ +L++ QR +RD + Q D + +DS Sbjct: 182 LASDAAYLKRVWTKVMERKKRPQTRYQMYGELALAQRVLRDFADAQLDRIRVDSRLTYES 241 Query: 250 AEAFLNDMAPGKYKPVRFYHGQESIFTAYGVQEQLHKSFGRKITLEGGATLIWDETEALT 309 F + P + Y G + IF Y V+ ++ ++ RK+ L+ G LI D+TEA+T Sbjct: 242 LLEFTAEYIPEMTSKLEHYSGHQPIFDLYDVENEIQRALERKVELKSGGYLIIDQTEAMT 301 Query: 310 VIDVNTAQYTGGTSLEETVTRTNLLAAEEIGRLIRLRDTXXXXXXXXXXMQREEHRKMVT 369 +D+NT + G +L++T+ TN+ A + I R +RLR+ M E+HR+ V Sbjct: 302 TVDINTGAFVGHRNLDDTIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMNNEDHRRRVL 361 Query: 370 ERLERVISRDRTQTHILGWTHLGLLEMTRKKARHDSAGFAPAICQCCGGTGKV 422 LE+ +S+DR +T I G++ LGL+EMTRK+ R C C G G V Sbjct: 362 HSLEQALSKDRVKTSINGFSPLGLVEMTRKRTRESVEHVLCNECPTCHGRGTV 414 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,084,105 Number of extensions: 1232879 Number of successful extensions: 2540 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 2488 Number of HSP's successfully gapped: 29 Length of query: 427 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 318 Effective length of database: 25,807,025 Effective search space: 8206633950 Effective search space used: 8206633950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)