BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0006.p (428 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3422 [E] COG0460 Homoserine dehydrogenase 409 e-114 MTH1232 [E] COG0460 Homoserine dehydrogenase 245 1e-64 BH1253 [E] COG0460 Homoserine dehydrogenase 147 3e-35 MTH417 [E] COG0460 Homoserine dehydrogenase 135 2e-31 BH1737 [E] COG0460 Homoserine dehydrogenase 111 2e-24 >BH3422 [E] COG0460 Homoserine dehydrogenase Length = 431 Score = 409 bits (1050), Expect = e-114, Method: Compositional matrix adjust. Identities = 199/409 (48%), Positives = 280/409 (68%) Query: 20 RIVEGNQEDLSSQVGSPILIERIAVKNTDKPRDIEVDAAAITDDPWDVIRDPEIDVIVEV 79 +++E +Q++L Q+G P+ +E++ V++ K RD+ + ++T +++ + IDV++EV Sbjct: 21 KLIENHQQELRHQIGLPVQVEKVLVRDRSKARDVVLSEESVTLYGEEIVENANIDVVIEV 80 Query: 80 MGGIAGTKEYILEALERGKHIVTANKDLMALHGSEILAKAQEKQCDVFYEASVAGGIPII 139 MGGI KEYI+ AL+ GKH+VTANKDLMA+HG+E+L+ A E CD+FYEASVAGGIPII Sbjct: 81 MGGITLAKEYIVRALKNGKHVVTANKDLMAVHGAELLSLAAENGCDLFYEASVAGGIPII 140 Query: 140 RTLIEGFSSDKIVKIMGIVNGTTNYILSKMSQEGASYHDVLKEAQELGYAESDPTSDVEG 199 R+L +G ++D+I K+MGIVNGTTNYIL+KM++EG Y +VL +AQ LG+AESDPT+DVEG Sbjct: 141 RSLTDGLAADRITKMMGIVNGTTNYILTKMTKEGLDYEEVLAQAQALGFAESDPTADVEG 200 Query: 200 LDAARKMAILGTLGFRTNVELXXXXXXXXXXXXKEDIAFAKRLGYEMKLLGIAESHEDEF 259 LDAARKMAIL TLGF +VEL K D+ + ++LGY MKL+G+A+ D Sbjct: 201 LDAARKMAILATLGFSMDVELDDVVVRGISSVTKTDLEYCEQLGYTMKLIGLADRDGDRV 260 Query: 260 SISVQPTMIRTGHPIASXXXXXXXXXXXXXXXXETMFYGAGAGAMPTATSIVADLVAVIK 319 +SVQPT++ HP+A+ ETMFYG GAG++PTAT++V+DLV+V+K Sbjct: 261 EVSVQPTLLPKEHPLAAVNDEYNAVYVYGEAVGETMFYGPGAGSLPTATAVVSDLVSVLK 320 Query: 320 NLKLGVNGLKQIVPYKQKKLKSDEDIFYKNFLLLHVDDKAGVLAKITQVFAEYDVSLDSV 379 N++ VNG P +K LK+D ++F K F L V DKAG + IT +FAE DVS + + Sbjct: 321 NMRYRVNGHDGAKPLHKKTLKADNEVFAKYFFRLKVLDKAGTFSAITSLFAEKDVSFEKL 380 Query: 380 VQQANPSNPDAEIIIVTHNASKASMNKVMRHLEQLKVIHRIKSHYRVEG 428 +Q AEIII+TH K + + R L L V+ ++S YRVEG Sbjct: 381 LQLPIKGEAAAEIIIITHTTDKETYEYLHRTLAALDVVQSVESSYRVEG 429 >MTH1232 [E] COG0460 Homoserine dehydrogenase Length = 268 Score = 245 bits (625), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 0/401 (0%), Positives = 0/401 (0%), Gaps = 9/401 (2%) Query: 21 IVEGNQEDLSSQVGSPILIERIAVKNTDKPRDIEVDAAAITDDPWDVIRDPEIDVIVEVM 80 + + + + + D + +A I D + + V + Sbjct: 18 TGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMDTPQYLRRKVFPI 77 Query: 81 GGIAGTKEYILEALERGKHIVTANKDLMALHGSEILAKAQEKQCDVFYEASVAGGIPIIR 140 + + K + ++ + Sbjct: 78 MRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIGADKLALCREKLTVN 137 Query: 141 TLIEGFSSDKIVKIMGIVNGTTNYILSKMSQEGASYHDVLKEAQELGYAESDPTSDVEGL 200 ++ +I+ + + + + + + Sbjct: 138 RIMSFRVVRLGKEILIEPDEPEDRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPIT 197 Query: 201 DAARKMAILGTLGFRTNVELXXXXXXXXXXXXKEDIAFAK-RLGYEMKLLGIAESHEDEF 259 ++ + + + ++ + F Sbjct: 198 SILDEVKTRMRGAREAAILFGGPYKGLPEIDADIWVNTLPGQCTETVRTEEAVLATLSVF 257 Query: 260 SISVQPTMIRTGHPIASXXXXXXXXXXXXXXXXETMFYGAGAGAMPTATSIVADLVAVIK 319 Sbjct: 258 317 Query: 320 NLKLGV---NGLKQIVPYKQKKLKSDEDIFYKNFLLLHVDDKAGVLAKITQVFAEYDVSL 376 Sbjct: 318 ----- 372 Query: 377 DSVVQQANPSNPDAEIIIVTHNASKASMNKVMRHLEQLKVI 417 Sbjct: 373 413 >BH1253 [E] COG0460 Homoserine dehydrogenase Length = 417 Score = 147 bits (372), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 94/279 (33%), Positives = 133/279 (47%), Gaps = 2/279 (0%) Query: 37 ILIERIAVKNTDKPRDIEVDAAAITDDPWDVIRDPEIDVIVEVMGGIAGTKEYILEALER 96 + +E + V N K E A +TDD W+ DV+ E GGI +Y+ L++ Sbjct: 31 VTLEMLLVTNRRKHEHSE-QYALVTDD-WETFAARYYDVVFEAAGGIEPVYDYVRFFLKK 88 Query: 97 GKHIVTANKDLMALHGSEILAKAQEKQCDVFYEASVAGGIPIIRTLIEGFSSDKIVKIMG 156 G +VTANK L+A G E+ A E + YEA+VAGGIPI+ + ++ + G Sbjct: 89 GVPVVTANKKLVAERGEELERLAAENDTYLCYEAAVAGGIPILNAIKGTLATTSFTSVAG 148 Query: 157 IVNGTTNYILSKMSQEGASYHDVLKEAQELGYAESDPTSDVEGLDAARKMAILGTLGFRT 216 I+NGTTNYIL+ M ++ + D+L+EAQ GYAE+DPT D+EG DA K+ IL L F Sbjct: 149 ILNGTTNYILTGMIEQNRPFQDMLEEAQAKGYAEADPTDDIEGYDAWYKIRILARLCFGI 208 Query: 217 NVELXXXXXXXXXXXXKEDIAFAKRLGYEMKLLGIAESHEDEFSISVQPTMIRTGHPIAS 276 + + LG +KL G A + SV P HP ++ Sbjct: 209 WPNRDDISRSGLHDVLDWHVELGQSLGLTLKLDGRASVKNNTIIGSVAPAFFTNDHPFST 268 Query: 277 XXXXXXXXXXXXXXXXETMFYGAGAGAMPTATSIVADLV 315 +F G GAG TA S+V D + Sbjct: 269 VYGVTNAVVLEGKAIERLLFTGPGAGKEATANSMVEDYL 307 >MTH417 [E] COG0460 Homoserine dehydrogenase Length = 100 Score = 135 bits (339), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 0/233 (0%), Positives = 0/233 (0%), Gaps = 5/233 (2%) Query: 87 KEYILEALERGKHIVTANKDLMALHGSEILAKAQEKQCDVFYEASVAGGIPIIRTLIEGF 146 Sbjct: 108 167 Query: 147 SSDKIVKIMGIVNGTTNYILSKMSQEGASYHDVLKEAQELGYAESDPTSDVEGLDAARKM 206 Sbjct: 168 227 Query: 207 AILGTLGFRTNVELXXXXXXXXXXXXKEDIAFAKRLGYEMKLLGIAESHEDEFSISVQPT 266 Sbjct: 228 ---- 283 Query: 267 MIRTGHPIASXXXXXXXXXXXXXXXXETMFYGAGAGAMPTATSIVADLVAVIK 319 Sbjct: 284 - 335 >BH1737 [E] COG0460 Homoserine dehydrogenase Length = 342 Score = 111 bits (277), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 79/259 (30%), Positives = 120/259 (46%), Gaps = 5/259 (1%) Query: 63 DPWDVIRDPEIDVIVEVMGGIAGTKE----YILEALERGKHIVTANKDLMALHGSEILAK 118 + ++ I+ D IVEV T + + EAL GKH+VT NK +A+ E+ Sbjct: 80 NSFETIQQTNADTIVEVTFTDVLTGQPAIDHCKEALRLGKHVVTTNKGPIAVAYQELKEL 139 Query: 119 AQEKQCDVFYEASVAGGIPIIRTLIEGFSSDKIVKIMGIVNGTTNYILSKMSQEGASYHD 178 A + YE +V G P +R + I ++ GI+NGTTNYIL++M + G ++ Sbjct: 140 ADANGVQLLYEGTVMSGTPALRLPEHTLIGNNIQEVKGILNGTTNYILTEM-ERGKTFEQ 198 Query: 179 VLKEAQELGYAESDPTSDVEGLDAARKMAILGTLGFRTNVELXXXXXXXXXXXXKEDIAF 238 L++AQ LGYAE+DPTSDVEG D K+ IL + ++ DI Sbjct: 199 ALQDAQTLGYAEADPTSDVEGYDVLYKVVILANVMMGIPLKPSDVERVGISHLTPRDIKD 258 Query: 239 AKRLGYEMKLLGIAESHEDEFSISVQPTMIRTGHPIASXXXXXXXXXXXXXXXXETMFYG 298 A G KL+G + + +V+P + P+A+ G Sbjct: 259 AHSAGERWKLIGRVKKEGERTMATVRPERLPLTDPLANINGAINAITYECDISGPITLIG 318 Query: 299 AGAGAMPTATSIVADLVAV 317 AGAG T +++ DL+ V Sbjct: 319 AGAGVKETGFALLIDLLHV 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,501,066 Number of extensions: 1033453 Number of successful extensions: 2503 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 2492 Number of HSP's successfully gapped: 19 Length of query: 428 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 319 Effective length of database: 25,807,025 Effective search space: 8232440975 Effective search space used: 8232440975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)