BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0368.0003.p (293 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0567 [EH] COG0115 Branched-chain amino acid aminotransferase/... 198 1e-50 ZilvE [EH] COG0115 Branched-chain amino acid aminotransferase/4-... 196 4e-50 ECs4704 [EH] COG0115 Branched-chain amino acid aminotransferase/... 196 4e-50 ZilvE [EH] COG0115 Branched-chain amino acid aminotransferase/4-... 195 6e-50 ilvE [EH] COG0115 Branched-chain amino acid aminotransferase/4-a... 195 6e-50 >RSc0567 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 309 Score = 198 bits (503), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 112/296 (37%), Positives = 169/296 (57%), Gaps = 10/296 (3%) Query: 6 IYLDGQHVTKENAKVSVFDHGFLYGDGIFEGIRIYNG----NIFKCKEHLDRLYDSAKSI 61 I+ DG+ + +A++ V H YG G+FEG+R Y IF+ EH RL++SAK Sbjct: 11 IWKDGKLIEWRDAQLHVLTHTLHYGMGVFEGVRAYQTPAGPAIFRLSEHTRRLFNSAKIF 70 Query: 62 MLDIPLTYDEMLEAMAETIRLNDMRNGYIRLIVSRGPGNLGLDPRRCPKASVIIIVEQLA 121 + IP + ++++A E +R N + + YIR + G +G+ R I A Sbjct: 71 QMQIPFSEQQLMDAQREVVRANALASCYIRPLAWLGSEKMGVSARGNTVHVAIAAWPWGA 130 Query: 122 IYPEQAYLNGLRAVSVS-QRRNIPDALNPKIKSLNYLNNILVKIQSNLAEADEAIMMNAQ 180 E+ G+R + S R ++ +L S Y+N+IL ++ DEA++++ + Sbjct: 131 YLGEEGMQRGIRVKTSSFTRHHVNVSLVRAKASGYYINSILANQEATGLGYDEALLLDTE 190 Query: 181 GYVTEGSGDNIFIVKRGIVYTPPCYLGALEGITRLAIIELCGKLGLTLKEEPFTMHDVYI 240 GYV+EGSG+N+FIV+ G++YTP L+GITR AII + G LG+ ++E+ T ++Y Sbjct: 191 GYVSEGSGENVFIVRNGVIYTPDL-ASCLDGITRDAIITIAGDLGIEVREKRITRDEMYC 249 Query: 241 ADEVFFTGTAAEVIAAREIDGRVIGAGHAGPITLQLLEEFRNVVDK----DGYKVW 292 ADE FFTGTAAEV RE+D RVIGAG GPIT QL F ++V+ + YK W Sbjct: 250 ADEAFFTGTAAEVTPIRELDDRVIGAGSRGPITTQLQSTFFDIVNGKSTIEKYKAW 305 >ZilvE [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 309 Score = 196 bits (498), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 107/299 (35%), Positives = 173/299 (57%), Gaps = 16/299 (5%) Query: 5 WIYLDGQHVTKENAKVSVFDHGFLYGDGIFEGIRIYNGN----IFKCKEHLDRLYDSAKS 60 +I+ +G+ V E+AKV V H YG +FEGIR Y+ + +F+ +EH+ RL+DSAK Sbjct: 8 YIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQRLHDSAKI 67 Query: 61 IMLDIPLTYDEMLEAMAETIRLNDMRNGYIRLIVSRGPGNLGLDPRRCPKASVIIIVEQL 120 + + DE++EA + IR N++ + YIR ++ G +G++P VII Sbjct: 68 YRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDVII----- 122 Query: 121 AIYPEQAYL------NGLRAVSVSQRRNIPDALNPKIKSL-NYLNNILVKIQSNLAEADE 173 A +P AYL G+ A+ S R P+ + K+ NYL+++LV ++ E Sbjct: 123 AAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQE 182 Query: 174 AIMMNAQGYVTEGSGDNIFIVKRGIVYTPPCYLGALEGITRLAIIELCGKLGLTLKEEPF 233 I ++ GY++EG+G+N+F VK G+++TPP AL GITR AII+L +LG+ ++E+ Sbjct: 183 GIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQVL 242 Query: 234 TMHDVYIADEVFFTGTAAEVIAAREIDGRVIGAGHAGPITLQLLEEFRNVVDKDGYKVW 292 + +Y+ADEVF +GTAAE+ R +DG +G G GP+T ++ + F + + W Sbjct: 243 SRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDKW 301 >ECs4704 [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 309 Score = 196 bits (498), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 107/299 (35%), Positives = 173/299 (57%), Gaps = 16/299 (5%) Query: 5 WIYLDGQHVTKENAKVSVFDHGFLYGDGIFEGIRIYNGN----IFKCKEHLDRLYDSAKS 60 +I+ +G+ V E+AKV V H YG +FEGIR Y+ + +F+ +EH+ RL+DSAK Sbjct: 8 YIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQRLHDSAKI 67 Query: 61 IMLDIPLTYDEMLEAMAETIRLNDMRNGYIRLIVSRGPGNLGLDPRRCPKASVIIIVEQL 120 + + DE++EA + IR N++ + YIR ++ G +G++P VII Sbjct: 68 YRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDVII----- 122 Query: 121 AIYPEQAYL------NGLRAVSVSQRRNIPDALNPKIKSL-NYLNNILVKIQSNLAEADE 173 A +P AYL G+ A+ S R P+ + K+ NYL+++LV ++ E Sbjct: 123 AAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQE 182 Query: 174 AIMMNAQGYVTEGSGDNIFIVKRGIVYTPPCYLGALEGITRLAIIELCGKLGLTLKEEPF 233 I ++ GY++EG+G+N+F VK G+++TPP AL GITR AII+L +LG+ ++E+ Sbjct: 183 GIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQVL 242 Query: 234 TMHDVYIADEVFFTGTAAEVIAAREIDGRVIGAGHAGPITLQLLEEFRNVVDKDGYKVW 292 + +Y+ADEVF +GTAAE+ R +DG +G G GP+T ++ + F + + W Sbjct: 243 SRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDKW 301 Score = 195 bits (496), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 107/299 (35%), Positives = 173/299 (57%), Gaps = 16/299 (5%) Query: 5 WIYLDGQHVTKENAKVSVFDHGFLYGDGIFEGIRIYNGN----IFKCKEHLDRLYDSAKS 60 +I+ +G+ V E+AKV V H YG +FEGIR Y+ + +F+ +EH+ RL+DSAK Sbjct: 8 YIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQRLHDSAKI 67 Query: 61 IMLDIPLTYDEMLEAMAETIRLNDMRNGYIRLIVSRGPGNLGLDPRRCPKASVIIIVEQL 120 + + DE++EA + IR N++ + YIR ++ G +G++P VII Sbjct: 68 YRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDVII----- 122 Query: 121 AIYPEQAYL------NGLRAVSVSQRRNIPDALNPKIKSL-NYLNNILVKIQSNLAEADE 173 A +P AYL G+ A+ S R P+ + K+ NYL+++LV ++ E Sbjct: 123 AAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGYQE 182 Query: 174 AIMMNAQGYVTEGSGDNIFIVKRGIVYTPPCYLGALEGITRLAIIELCGKLGLTLKEEPF 233 I ++ GY++EG+G+N+F VK G+++TPP AL GITR AII+L +LG+ ++E+ Sbjct: 183 GIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQVL 242 Query: 234 TMHDVYIADEVFFTGTAAEVIAAREIDGRVIGAGHAGPITLQLLEEFRNVVDKDGYKVW 292 + +Y+ADEVF +GTAAE+ R +DG +G G GP+T ++ + F + + W Sbjct: 243 SRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDKW 301 >ilvE [EH] COG0115 Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase Length = 301 Score = 195 bits (496), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 11/299 (3%), Positives = 50/299 (16%), Gaps = 16/299 (5%) Query: 5 WIYLDGQHVTKENAKVSVFDHGFLYGDGIFEGIRIYNGN----IFKCKEHLDRLYDSAKS 60 ++ + + + + Sbjct: 8 CGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIYVDTPGLH 67 Query: 61 IMLDIPLTYDEMLEAMAETIRLNDMRNGYIRLIVSRGPGNLGLDPRRCPKASVIIIVEQL 120 + + A + + + + + R ++ Sbjct: 68 MEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILA----- 122 Query: 121 AIYPEQAYL------NGLRAVSVSQRRNIPDALNPKIKSL-NYLNNILVKIQSNLAEADE 173 + + S +I ++ + + E Sbjct: 123 VNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPE 182 Query: 174 AIMMNAQGYVTEGSGDNIFIVKRGIVYTPPCYLGALEGITRLAIIELCGKLGLTLKEEPF 233 + + +++ P +E + ++ E Sbjct: 183 DYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQ 242 Query: 234 TMHDVYIADEVFFTGTAAEVIAAREIDGRVIGAGHAGPITLQLLEEFRNVVDKDGYKVW 292 + T +E+ + + ++ R + Sbjct: 243 KKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDDL 301 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.141 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,669,310 Number of extensions: 840880 Number of successful extensions: 8532 Number of sequences better than 1.0e-05: 58 Number of HSP's gapped: 8445 Number of HSP's successfully gapped: 58 Length of query: 293 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 187 Effective length of database: 26,153,027 Effective search space: 4890616049 Effective search space used: 4890616049 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)