BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0019.p (719 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_nasD [C] COG1251 NAD(P)H-nitrite reductase 222 2e-57 RSp1220 [C] COG1251 NAD(P)H-nitrite reductase 197 6e-50 TM0395 [R] COG0446 Uncharacterized NAD(FAD)-dependent dehydrogen... 192 1e-48 BS_nasB [C] COG1251 NAD(P)H-nitrite reductase 192 2e-48 PA1781 [C] COG1251 NAD(P)H-nitrite reductase 189 1e-47 >BS_nasD [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 222 bits (565), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 0/363 (0%), Positives = 0/363 (0%), Gaps = 3/363 (0%) Query: 3 KHYVIVGSGVAAVHAAKAIRDQDPDT-EISIFGEEAHLPYNRIKLTKGLFTDLHSDKVLI 61 V+ + + + + A + ++ + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 62 KKEKWYKDNRITVHTSTRVTHVNTDRRQVETADGRQIAYHKLLLCMGARNRALPVEGAEL 121 E + + + ++ + D L ++ + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 122 KNVHTLRDLQDADRLKASLESGNRIVVIGGGVQGLETAWALHEAGYEVTVVEAAPRLMIR 181 + + + G V L+T AL E + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 182 QLDETSSQMLKETLEQAGVKVILHSGVTSITGEEAVTGVTLDDETTVACDHVVYSIGIVP 241 + + V + + + + E + V+ + + + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 242 NTTLVKDTPIDTRSGIIVNPNMETSAPHVYAAGDIAEMNGQVEGLWGGAMEQGRVAGNNM 301 + A + A+ R Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 302 AASEPAAYRKAVPVTFFNAFGISLFSIGMTVESQCD--LSVSAQENGIYTQIFVKDHTIT 359 + A + + + +E+ Sbjct: 304 STLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQVY 363 Query: 360 GAI 362 Sbjct: 364 366 >RSp1220 [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 197 bits (500), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 0/376 (0%), Positives = 0/376 (0%), Gaps = 3/376 (0%) Query: 6 VIVGSGVAAVHAAKAIRDQDPDT-EISIFGEEAHLPYNRIKLTKGLFTDLHSDKVLIKKE 64 + S + ++ + D + + + + + + Sbjct: 10 SLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYV 69 Query: 65 KWYKDNRITVHTSTRVTHVNTDRRQVETADGRQIAYHKLLLCMGARNRALPVEGAELKNV 124 + + + V+ R + A + + + ++ L Sbjct: 70 GNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLY 129 Query: 125 HTLRDLQDADRLKASLESGNRIVVIGGGVQGLETAWALHEAGYEVTVVEAAPRLMIRQLD 184 + + S + L+ AG + + Sbjct: 130 QVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRY 189 Query: 185 ETSSQMLKETLEQAGVKVILHSGVTSITGEEAVTGVTLDDETTVACDHVVYSIGIVPNTT 244 + + G V+ + +C G N Sbjct: 190 LHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGA 249 Query: 245 LVKDTPIDTRSGIIVNPNMETSAPHVYAAGDIAEMNGQVEGLWGGAMEQGRVAGNNMAAS 304 S +T + + + + + + Sbjct: 250 KPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGA 309 Query: 305 EPAAYRKAVPVTFFNAFGISLFSIGMTV--ESQCDLSVSAQENGIYTQIFVKDHTITGAI 362 K + + + V Sbjct: 310 TTMDQLKTNIESLHLELSEDVLAEIEAVHQ 369 Query: 363 SWEGAAASLVYKSAIE 378 Sbjct: 370 385 >TM0395 [R] COG0446 Uncharacterized NAD(FAD)-dependent dehydrogenases Length = 425 Score = 192 bits (489), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 122/369 (33%), Positives = 198/369 (53%), Gaps = 11/369 (2%) Query: 4 HYVIVGSGVAAVHAAKAIRDQDPDTEISIFGEEAHLPYNRIKLTKGLFTDLHSDKVLIKK 63 YVIVGSG A ++A +AIR+ D + EI + E ++ Y+R +T L + +K+ + Sbjct: 2 RYVIVGSGPAGLNAIEAIREVDKEGEILLITAEKYVGYSRPLITYLLGRKVTEEKMYYRT 61 Query: 64 EKWYKDNRITVHTSTRVTHVNTDRRQVETADGRQIAYHKLLLCMGARNRALPVEG-AELK 122 E + ++ R+ + +TRV V + + V T G +I Y KLL+ G + +EG K Sbjct: 62 EDYLREMRVDIKPATRVEKVIPEEKTVVTDSGEEIRYDKLLIATGGKPFVPNIEGLTGKK 121 Query: 123 NVHTLRDLQDADRLKASLESGN--RIVVIGGGVQGLETAWALHEAGYEVTVVEAAPRLMI 180 V T +D ++++ +E + VV+GGG+ GL+T AL E G +VT+VE A R++ Sbjct: 122 GVFTFTTWEDEEKVEKYIEENDVKEAVVLGGGLIGLKTTEALMELGVKVTIVELADRILS 181 Query: 181 RQLDETSSQMLKETLEQAGVKVILHSGVTSITGEEAVTGVTLDDETTVACDHVVYSIGIV 240 D +S+++ E L++ G VI + V + G++ V+ V L + +V +IG+ Sbjct: 182 VTFDRKASEIITEALKKEGCSVITNDTVVKVNGDDTVSSVVLKSGKEIPTKLLVIAIGVK 241 Query: 241 PNTTLVKDTPIDTRSGIIVNPNMETSAPHVYAAGDIAEMNGQVEG------LWGGAMEQG 294 PN +KD+ I+ GI+VN MET+ VYAAGD E ++G +W A+ QG Sbjct: 242 PNVEFLKDSGIEINRGIVVNEKMETNVEGVYAAGDCTEFYDLIDGQRKTIAIWPVAVAQG 301 Query: 295 RVAGNNMAASEPAAYRKAVPVTFFNAFGISLFSIGMT-VESQCDLSVSAQENGIYTQIFV 353 RVAG NMA Y +P+ GI S+G + VE ++ +E Y ++ + Sbjct: 302 RVAGYNMAGKN-VRYPGGIPMNSVELAGIPTISVGHSNVEDGGYEILTFEEGNTYKKMVL 360 Query: 354 KDHTITGAI 362 KD+ + GAI Sbjct: 361 KDNRLVGAI 369 >BS_nasB [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 192 bits (487), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 0/357 (0%), Positives = 0/357 (0%), Gaps = 4/357 (1%) Query: 3 KHYVIVGSGVAAVHAAKAIRDQDPDT-EISIFGEEAHLPYNRIKLTKGLFTDLHSDKVLI 61 V+ + + + + A + ++ + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 62 KKEKWYKDNRITVHTSTRVTHVNTDRRQVETADGRQIAYHKLLLCMGARNRALPVEGAEL 121 E + + + ++ + D L ++ + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 122 KNVHTLRDLQDADRLKASLESGNRIVVIGGGVQGLETAWALHEAGYEVTVVEAAPRLMIR 181 + + + G V L+T AL E + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 182 QLDETSSQMLKETLEQAGVKVILHSGVTSITGEEAVTGVTLDDETTVACDHVVYSIGIVP 241 + + V + + + + E + V+ + + + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 242 NTTLVKDTPIDTRSGIIVNPNMETSAPHVYAAGDIAEMNGQVEGLWGGAMEQGRVAGNNM 301 + A + A+ R Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 302 AASEPAAYRKAVPVTFFNAFGISLFSIGMTVESQ--CDLSVSAQENGIYTQ-IFVKD 355 + A + + + +E+ Sbjct: 304 STLLGATTMDQLKTNIESLHLELSEDVLAEIEAVHQ 360 >PA1781 [C] COG1251 NAD(P)H-nitrite reductase Length = 346 Score = 189 bits (481), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 0/361 (0%), Positives = 0/361 (0%), Gaps = 5/361 (1%) Query: 6 VIVGSGVAAVHAAKAIRDQDPD-TEISIFGEEAHLPYNRIKLTKGLFTDLHSDKVLIKKE 64 V+ + + + + A + ++ + E Sbjct: 7 PHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTE 66 Query: 65 KWYKDNRITVHTSTRVTHVNTDRRQVETADGRQIAYHKLLLCMGARNRALPVEGAELKNV 124 + + + ++ + D L ++ + + Sbjct: 67 TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 125 HTLRDLQDADRLKASLESGNRIVVIGGGVQGLETAWALHEAGYEVTVVEAAPRLMIRQLD 184 + + G V L+T AL E + Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 185 ETSSQMLKETLEQAGVKVILHSGVTSI--TGEEAVTGVTLDDETTVACDHVVYSIGIVPN 242 + + V + + + A E ++ Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 243 TTLVKDTPIDTRSGIIVNPNMETSAPHVYAAGDIAEMNGQVEGLWGGAMEQGRVAGNNMA 302 +T + E + V A DIA +G A + + + Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 303 ASEPAAYRKAVPVTFFNAFGISLFSIGMTV--ESQCDLSVSAQENGIYTQIFVKDHTITG 360 + + + + Sbjct: 307 LGATTMDQLKTNIESLHLELSEDVLAEIEAVH 366 Query: 361 A 361 Sbjct: 367 367 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 50,060,531 Number of extensions: 2039984 Number of successful extensions: 6891 Number of sequences better than 1.0e-05: 280 Number of HSP's gapped: 6724 Number of HSP's successfully gapped: 280 Length of query: 719 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 605 Effective length of database: 25,230,355 Effective search space: 15264364775 Effective search space used: 15264364775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)