BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0015.p (473 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0950 [C] COG5013 Nitrate reductase alpha subunit 452 e-127 STM1577 [C] COG5013 Nitrate reductase alpha subunit 447 e-125 STM1764 [C] COG5013 Nitrate reductase alpha subunit 446 e-125 ZnarG [C] COG5013 Nitrate reductase alpha subunit 443 e-124 narG [C] COG5013 Nitrate reductase alpha subunit 443 e-124 >BMEII0950 [C] COG5013 Nitrate reductase alpha subunit Length = 346 Score = 452 bits (1163), Expect = e-127, Method: Compositional matrix adjust. Identities = 0/472 (0%), Positives = 0/472 (0%), Gaps = 4/472 (0%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 Sbjct: 441 500 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 Sbjct: 501 -- 558 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 Sbjct: 559 618 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 Sbjct: 619 678 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 Sbjct: 679 738 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 Sbjct: 739 -- 796 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 Sbjct: 797 856 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLE 472 Sbjct: 857 908 >STM1577 [C] COG5013 Nitrate reductase alpha subunit Length = 346 Score = 447 bits (1150), Expect = e-125, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 Sbjct: 768 827 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 Sbjct: 828 -- 885 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 Sbjct: 886 - 944 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 Sbjct: 945 1004 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 Sbjct: 1005 1064 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 Sbjct: 1065 -- 1122 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 Sbjct: 1123 1182 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLED 473 Sbjct: 1183 1235 >STM1764 [C] COG5013 Nitrate reductase alpha subunit Length = 346 Score = 446 bits (1148), Expect = e-125, Method: Compositional matrix adjust. Identities = 0/472 (0%), Positives = 0/472 (0%), Gaps = 5/472 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 Sbjct: 769 828 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 Sbjct: 829 -- 886 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 Sbjct: 887 - 945 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 Sbjct: 946 1005 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 Sbjct: 1006 1065 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 Sbjct: 1066 -- 1123 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 Sbjct: 1124 1183 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLE 472 Sbjct: 1184 1235 >ZnarG [C] COG5013 Nitrate reductase alpha subunit Length = 346 Score = 443 bits (1139), Expect = e-124, Method: Compositional matrix adjust. Identities = 0/472 (0%), Positives = 0/472 (0%), Gaps = 5/472 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 Sbjct: 769 828 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 Sbjct: 829 -- 886 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 Sbjct: 887 - 945 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 Sbjct: 946 1005 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 Sbjct: 1006 1065 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 Sbjct: 1066 -- 1123 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 Sbjct: 1124 1183 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLE 472 Sbjct: 1184 1235 >narG [C] COG5013 Nitrate reductase alpha subunit Length = 1247 Score = 443 bits (1139), Expect = e-124, Method: Compositional matrix adjust. Identities = 216/472 (45%), Positives = 310/472 (65%), Gaps = 5/472 (1%) Query: 1 MVALDFRMTTTPLYADIVLPAATWYEKTDLSSTDMHPFVHPFNPAVNPLWESRSDWDIYR 60 +V LDFR+++T LY+DI+LP ATWYEK D++++DMHPF+HP + AV+P WE++SDW+IY+ Sbjct: 769 VVTLDFRLSSTCLYSDIILPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWEAKSDWEIYK 828 Query: 61 QLAEVFSEMAKSELPGVYKDLVASPLAHDSISEISQPMGLVKDWAKGEVEAVPGKTMPNL 120 +A+ FSE+ L G D+V P+ HDS +E++QP+ VKDW KGE + +PGKT P++ Sbjct: 829 AIAKKFSEVCVGHL-GKETDIVTLPIQHDSAAELAQPLD-VKDWKKGECDLIPGKTAPHI 886 Query: 121 SIVERDYTKIHDKYISLGPNLVVGKAGAHGISFSVAEEYEELKRLNGVFYDESIKNGLPK 180 +VERDY ++++ S+GP + G GI+++ E + L++LN E G P Sbjct: 887 MVVERDYPATYERFTSIGPLMEKIGNGGKGIAWNTQSEMDLLRKLN-YTKAEGPAKGQPM 945 Query: 181 IQTARQVADTILHLSSATNGRVSQKAYEQAEKDSGVVLRDISADRAAEKITFQSITAQPR 240 + TA A+ IL L+ TNG+V+ KA+ + +G ++ ++ EKI F+ I AQPR Sbjct: 946 LNTAIDAAEMILTLAPETNGQVAVKAWAALSEFTGRDHTHLALNKEDEKIRFRDIQAQPR 1005 Query: 241 EVIPTPVFXXXXKKPRRYSPFTTNIERLVPFRTLTGRQHFYIDHEIFQQFGEALPVYKPT 300 ++I +P + + Y+ TN+ L+P+RTL+GRQ Y DH+ + FGE+L VY+P Sbjct: 1006 KIISSPTWSGLEDEHVSYNAGYTNVHELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPP 1065 Query: 301 LPPMVFGPRDREVKGGQDALVLRYLTPHGKWNIHSTYQDNQHMLTLFRGGPTXXXXINNE 360 + + G L +LTPH KW IHSTY DN MLTL RGGP ++ Sbjct: 1066 IDTRSVKEVIGQKSNGNQEKALNFLTPHQKWGIHSTYSDNLLMLTLGRGGPVVW--LSEA 1123 Query: 361 DAEQHGISDNDWLEVYNRNGVVTARAVVSHRMPRGTMFMYHAQDKHIQVPGSEITDTRGG 420 DA+ GI+DNDW+EV+N NG +TARAVVS R+P G MYHAQ++ + +PGSEIT RGG Sbjct: 1124 DAKDLGIADNDWIEVFNSNGALTARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGG 1183 Query: 421 SHNAPTRIHLKPTQMVGGYAQLSYGFNYYGPIGNQRDVYVAVRLKKEVNWLE 472 HN+ TRI KPT M+GGYA L+YGFNYYG +G+ RD +V VR K ++WL+ Sbjct: 1184 IHNSVTRITPKPTHMIGGYAHLAYGFNYYGTVGSNRDEFVVVRKMKNIDWLD 1235 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,505,361 Number of extensions: 1486927 Number of successful extensions: 3250 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 3210 Number of HSP's successfully gapped: 18 Length of query: 473 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 363 Effective length of database: 25,691,691 Effective search space: 9326083833 Effective search space used: 9326083833 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)