BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0014.p (517 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZnarY [C] COG1140 Nitrate reductase beta subunit 614 e-176 narY [C] COG1140 Nitrate reductase beta subunit 614 e-176 STM1578 [C] COG1140 Nitrate reductase beta subunit 614 e-175 ZnarY [C] COG1140 Nitrate reductase beta subunit 612 e-175 ECs2070 [C] COG1140 Nitrate reductase beta subunit 612 e-175 >ZnarY [C] COG1140 Nitrate reductase beta subunit Length = 346 Score = 614 bits (1584), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGW-Q 59 + + + +Y + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 60 LRKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 + + S KL + K+ + + + Sbjct: 69 VGNWLAKHGSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 120 SAVTGEKMD-LEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHC 178 + E + + ++ Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 179 LNPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFC 238 + +A ++ L+++ G + + ++ + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 239 FPRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPE 298 AG T A + D + A Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 299 VIAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFE 358 Sbjct: 307 366 Query: 359 GKDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPF 418 Sbjct: 367 - 425 Query: 419 DLSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGY 471 Sbjct: 426 478 >narY [C] COG1140 Nitrate reductase beta subunit Length = 346 Score = 614 bits (1584), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGW-Q 59 + + + +Y + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 60 LRKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 + + S KL + K+ + + + Sbjct: 69 VGNWLAKHGSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 120 SAVTGEKMD-LEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHC 178 + E + + ++ Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 179 LNPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFC 238 + +A ++ L+++ G + + ++ + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 239 FPRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPE 298 AG T A + D + A Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 299 VIAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFE 358 Sbjct: 307 366 Query: 359 GKDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPF 418 Sbjct: 367 - 425 Query: 419 DLSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGY 471 Sbjct: 426 478 >STM1578 [C] COG1140 Nitrate reductase beta subunit Length = 346 Score = 614 bits (1584), Expect = e-175, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGW-Q 59 + + + +Y + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 60 LRKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 + + S KL + K+ + + + Sbjct: 69 VGNWLAKHGSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 120 SAVTGEKMD-LEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHC 178 + E + + ++ Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 179 LNPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFC 238 + +A ++ L+++ G + + ++ + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 239 FPRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPE 298 AG T A + D + A Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 299 VIAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFE 358 Sbjct: 307 366 Query: 359 GKDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPF 418 Sbjct: 367 - 425 Query: 419 DLSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGY 471 Sbjct: 426 478 Score = 612 bits (1579), Expect = e-175, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGW-Q 59 + + + +Y + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 60 LRKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 + + S KL + K+ + + + Sbjct: 69 VGNWLAKHGSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 120 SAVTGEKMD-LEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHC 178 + E + + ++ Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 179 LNPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFC 238 + +A ++ L+++ G + + ++ + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 239 FPRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPE 298 AG T A + D + A Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 299 VIAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFE 358 Sbjct: 307 366 Query: 359 GKDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPF 418 Sbjct: 367 - 425 Query: 419 DLSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGY 471 Sbjct: 426 478 >ECs2070 [C] COG1140 Nitrate reductase beta subunit Length = 346 Score = 612 bits (1579), Expect = e-175, Method: Compositional matrix adjust. Identities = 0/473 (0%), Positives = 0/473 (0%), Gaps = 5/473 (1%) Query: 1 MVMNLDKCIGCHTCSVTCKTTWTNRKGAEYMWFNNVETKPGIGYPKRWEDQELYKGGW-Q 59 + + + +Y + E Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 60 LRKGKLELKSGNKLSKVALGKIFYNPDMPEMKDYYEPWTYNYEHLTEAGEQKHSPVARAH 119 + + S KL + K+ + + + Sbjct: 69 VGNWLAKHGSREKLIIAS--KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYL 126 Query: 120 SAVTGEKMD-LEWGPNWEDDLAGAHVTGPLDPNIQKIEEEIKFNFEQSFMMYLPRLCEHC 178 + E + + ++ Sbjct: 127 DLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGV 186 Query: 179 LNPSCVASCPSGAMYKREEDGIVLVDQEACRGWRYCMTGCPYKKVYFNWQTNKAEKCTFC 238 + +A ++ L+++ G + + ++ + Sbjct: 187 MRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYL 246 Query: 239 FPRVEAGLPTVCSETCTGRIRYLGVLLYDADKVLDAASTPDVKDLYQAQCDLFMNPHDPE 298 AG T A + D + A Sbjct: 247 NGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTL 306 Query: 299 VIAQARKDGISEDWLEAAQNSPVYKLAIEHKLAFPLHPEYRTLPMVWYVPPLSPIMNYFE 358 Sbjct: 307 366 Query: 359 GKDSLKNPDMIFPAVEEMRTPIQYLANMLTSGDTQTVKEALQRMAMMRSYMRAKSADQPF 418 Sbjct: 367 - 425 Query: 419 DLSRLDRVGMTAHQTEEMYRLLAIAKYEDRFVIPTSHKEQTMNPYRAQGSAGY 471 Sbjct: 426 478 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,589,709 Number of extensions: 1723078 Number of successful extensions: 12868 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 12836 Number of HSP's successfully gapped: 70 Length of query: 517 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 406 Effective length of database: 25,576,357 Effective search space: 10384000942 Effective search space used: 10384000942 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)