BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0013.p (149 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT1200 [C] COG2180 Nitrate reductase delta subunit 52 3e-07 Rv1163 [C] COG2180 Nitrate reductase delta subunit 52 3e-07 STM1762 [C] COG2180 Nitrate reductase delta subunit 47 8e-06 >MT1200 [C] COG2180 Nitrate reductase delta subunit Length = 346 Score = 52.0 bits (123), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 2/60 (3%), Positives = 12/60 (20%) Query: 66 NYAATFDFQRDCALYMTYFKFEDAKERGQILAKLKILYEMYGLEMPEGELPDYLPLMCEF 125 T + + + + ++ + + + L + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 >Rv1163 [C] COG2180 Nitrate reductase delta subunit Length = 346 Score = 52.0 bits (123), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 3/60 (5%), Positives = 8/60 (13%) Query: 66 NYAATFDFQRDCALYMTYFKFEDAKERGQILAKLKILYEMYGLEMPEGELPDYLPLMCEF 125 + + K ++ P+ L Sbjct: 56 PPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH 115 >STM1762 [C] COG2180 Nitrate reductase delta subunit Length = 346 Score = 47.4 bits (111), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 4/65 (6%), Positives = 14/65 (21%) Query: 62 EIQENYAATFDFQRDCALYMTYFKFEDAKERGQILAKLKILYEMYGLEMPEGELPDYLPL 121 E T + + + + ++ + + + L Sbjct: 57 PRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHD 116 Query: 122 MCEFL 126 + L Sbjct: 117 SLKRL 121 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.140 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,579,582 Number of extensions: 446485 Number of successful extensions: 586 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 586 Number of HSP's successfully gapped: 7 Length of query: 149 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 52 Effective length of database: 27,191,033 Effective search space: 1413933716 Effective search space used: 1413933716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 111 (47.4 bits)