BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0006.p (313 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_narK [P] COG2223 Nitrate/nitrite transporter 269 5e-72 PA3877 [P] COG2223 Nitrate/nitrite transporter 107 2e-23 RSp0972 [P] COG2223 Nitrate/nitrite transporter 101 1e-21 BH0232 [P] COG2223 Nitrate/nitrite transporter 99 9e-21 Rv1737c [P] COG2223 Nitrate/nitrite transporter 99 1e-20 >BS_narK [P] COG2223 Nitrate/nitrite transporter Length = 395 Score = 269 bits (687), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 136/251 (54%), Positives = 169/251 (67%) Query: 3 KKLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGY 62 + +QL LQ+L+L+ GFMVWV+ISSL+ I+ DI + G++++VTAIPV+LGS+LRIP+GY Sbjct: 5 QHIQLSLQSLSLVAGFMVWVLISSLISQITLDIHLSKGEISLVTAIPVILGSLLRIPLGY 64 Query: 63 YANIFGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKYFP 122 N FGAR++ +IS A + D AVFS+GVTSLPKY+P Sbjct: 65 LTNRFGARLMFMVSFILLLFPVFWISIADSLFDLIAGGFFLGIGGAVFSIGVTSLPKYYP 124 Query: 123 KEKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDRHE 182 KEKHG VNGIYG GN+GTA+TTF AP+IA GW + V+ GDRHE Sbjct: 125 KEKHGVVNGIYGAGNIGTAVTTFAAPVIAQAVGWKSTVQMYLILLAVFALLHVLFGDRHE 184 Query: 183 PKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGMRT 242 KVK + QIK V +N LW SLFYFITFG+FVAFTIYLPNFLV +FGL DAG+RT Sbjct: 185 KKVKVSVKTQIKAVYRNHVLWFLSLFYFITFGAFVAFTIYLPNFLVEHFGLNPADAGLRT 244 Query: 243 AGFIAVATFFR 253 AGFIAV+T R Sbjct: 245 AGFIAVSTLLR 255 >PA3877 [P] COG2223 Nitrate/nitrite transporter Length = 431 Score = 107 bits (268), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 64/249 (25%), Positives = 111/249 (44%), Gaps = 12/249 (4%) Query: 7 LPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGYYANI 66 L + TL+ V F VW + S + I +++ + + ++ A+P++ GS++R+P+G + Sbjct: 12 LGMSTLSFAVNFAVWTMFSIIGIRIKDELGLSESQFGLMVALPILTGSLVRLPLGLITDR 71 Query: 67 FGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKYFPKEKH 126 FG RI+ ++ AS Y F+VG+ +F KE+ Sbjct: 72 FGGRIVFFIHMLLVAIPIYGLAFASQYWHYLVLGLFVGLAGGSFAVGIAYTSAWFEKERQ 131 Query: 127 GFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGDRHEPKVK 186 G GI+G GN G A+T AP+I FGW + +K Sbjct: 132 GTAMGIFGAGNAGAAITNLVAPMIVVAFGWRMVPQVYSVAMLVTAVLFWLFTWTDPAHLK 191 Query: 187 -------TPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAG 239 P + + + ++W F L+YF FG FVA ++LP + ++ +GL+ Sbjct: 192 GAAEASQRPNLAKQLAPLAELRVWRFGLYYFFVFGGFVALALWLPKYYIAEYGLD----- 246 Query: 240 MRTAGFIAV 248 ++TA FI + Sbjct: 247 LKTASFITM 255 >RSp0972 [P] COG2223 Nitrate/nitrite transporter Length = 437 Score = 101 bits (252), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 61/245 (24%), Positives = 109/245 (44%), Gaps = 10/245 (4%) Query: 7 LPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGYYANI 66 L T + F VW + + L + + + + + ++ A P++ GS+ R+P+G + + Sbjct: 25 LASSTFAFTICFAVWTMFAILGIPLQKQLGLSETEFGLIAATPILTGSLARVPLGMWTDR 84 Query: 67 FGARIIXXXXXXXXXXXXXYISEASTYTDXXXXXXXXXXXXAVFSVGVTSLPKYFPKEKH 126 FG RI+ +++EA+ FSVG + ++FPK + Sbjct: 85 FGGRIVFFLLMLATVVPIWFVAEATRLWQFLLLGMLVGLVGGSFSVGTPYVARWFPKSRQ 144 Query: 127 GFVNGIYGVGNLGTALTTFTAPIIAARFGWATAVKXXXXXXXXXXXXXXXXGD----RHE 182 GF GI+G GN G AL F AP++ G T V G H Sbjct: 145 GFAMGIFGAGNAGAALNKFVAPLLVVAAGTWTVVPRVYAVAMLVTALLFWFGSATNPAHA 204 Query: 183 PKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGMRT 242 + Q++ V+K+ ++W +S +Y + FG +V +++L + VS +G ++T Sbjct: 205 AGKSVSLRAQLR-VMKDPRVWRYSQYYSVVFGGYVGLSLWLTRYYVSEYGFS-----IKT 258 Query: 243 AGFIA 247 A F+A Sbjct: 259 AAFLA 263 >BH0232 [P] COG2223 Nitrate/nitrite transporter Length = 203 Score = 99.0 bits (245), Expect = 9e-21, Method: Compositional matrix adjust. Identities = 46/63 (73%), Positives = 55/63 (87%) Query: 191 EQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKVDAGMRTAGFIAVAT 250 EQ+K V +N LW FSLFYFITFG+FVAFTIYLPNFLV+++GL VD+G+RTAGFIA+AT Sbjct: 3 EQMKAVYRNRLLWFFSLFYFITFGAFVAFTIYLPNFLVNHYGLTPVDSGLRTAGFIALAT 62 Query: 251 FFR 253 F R Sbjct: 63 FIR 65 >Rv1737c [P] COG2223 Nitrate/nitrite transporter Length = 395 Score = 98.6 bits (244), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 69/257 (26%), Positives = 109/257 (42%), Gaps = 7/257 (2%) Query: 3 KKLQLPLQTLNLIVGFMVWVIISSLMPFISEDISIPSGKLAMVTAIPVVLGSILRIPIGY 62 + L L T +V F W +I L + D+S+ S + +++ A P+++G++ RI G Sbjct: 4 QAANLVLATWISVVNFWAWNLIGPLSTSYARDMSLSSAEASLLVATPILVGALGRIVTGP 63 Query: 63 YANIFGARIIXXXXXXXXXXXXXYISEAST---YTDXXXXXXXXXXXXAVFSVGVTSLPK 119 + FG R + + A+T Y +F+VG+ Sbjct: 64 LTDRFGGRAMLIAVTLASILPVLAVGVAATMGSYALLVFFGLFLGVAGTIFAVGIPFANN 123 Query: 120 YFPKEKHGFVNGIYGVGNLGTALTTFTAPIIAARFGWATA---VKXXXXXXXXXXXXXXX 176 ++ + GF G++G+G +GTAL+ F P FG T V Sbjct: 124 WYQPARRGFSTGVFGMGMVGTALSAFFTPRFVRWFGLFTTHAIVAAALASTAVVAMVVLR 183 Query: 177 XGDRHEPKVKTPIIEQIKGVIKNEKLWLFSLFYFITFGSFVAFTIYLPNFLVSNFGLEKV 236 P P++ ++K + W S Y I FG FVAF+ YLP ++ + +G V Sbjct: 184 DAPYFRPNAD-PVLPRLKAAARLPVTWEMSFLYAIVFGGFVAFSNYLPTYITTIYGFSTV 242 Query: 237 DAGMRTAGFIAVATFFR 253 DAG RTAGF A R Sbjct: 243 DAGARTAGFALAAVLAR 259 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.143 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,429,501 Number of extensions: 657037 Number of successful extensions: 1802 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1797 Number of HSP's successfully gapped: 17 Length of query: 313 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 207 Effective length of database: 26,153,027 Effective search space: 5413676589 Effective search space used: 5413676589 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)