BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0359.0003.p (345 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 237 2e-62 Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 237 2e-62 MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 235 9e-62 PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme 195 1e-49 Rv0869c [H] COG2896 Molybdenum cofactor biosynthesis enzyme 180 3e-45 >MT3192 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 368 Score = 237 bits (605), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 126/321 (39%), Positives = 191/321 (59%), Gaps = 13/321 (4%) Query: 13 IQDQLKRPIRDLRLSLTDRCNFRCSYCMPKEIFGEDYAFLPARELLSFAEILRLTRLFVS 72 ++D+ +R + DLRLS+ D+CN RC YCMP+E Y +LP ++LLS EI + +F+S Sbjct: 27 VKDRCRRMMGDLRLSVIDQCNLRCRYCMPEE----HYTWLPRQDLLSVKEISAIVDVFLS 82 Query: 73 LGVVKIRLTGGEPLLRKDLPELV----AGIRSLNGVEDIGLTTNGVLLGQQAKGLYAAGL 128 +GV K+R+TGGEPL+R DLPE+V A + +G+ D+ +TTNGVLL + GL AAG+ Sbjct: 83 VGVSKVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGM 142 Query: 129 RRLNISLDALHPEIFGTMNGRGLKPSLILKHIDQAREMGY-EIKVNMVVQKGVNESEILP 187 +R+ +SLD L PE F ++ R +I I G+ + K++ V +G N E+ Sbjct: 143 KRITVSLDTLQPERFKAISQRNSHDKVI-AGIKAVAAAGFTDTKIDTTVMRGANHDELAD 201 Query: 188 MAAYFKERKITLRFIEFMDAGNDNGWSLAKVVTKKEILQSLREVY-ELEALEQDFYGEVA 246 + + + +RFIE+MD G W+ KV TK +L+SL + Y +E L + A Sbjct: 202 LIEFARTVNAEVRFIEYMDVGGATHWAWEKVFTKANMLESLEKRYGRIEPLPKHDTAP-A 260 Query: 247 QRYRYKDSDAEIGFITSVSESFCSSCTRARLSSDGKFYTCLFASGGTDLRSLLRSGADDS 306 RY D G I S +E FC++C R+RL++DG + CL+A G +LR LR+GA Sbjct: 261 NRYALPDG-TTFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGINLREPLRAGATHD 319 Query: 307 SLLNVIKDVWEKRTDRYSDER 327 L+ + W +RTDR +++R Sbjct: 320 DLVETVTTGWRRRTDRGAEQR 340 >Rv3109 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 359 Score = 237 bits (605), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 126/321 (39%), Positives = 191/321 (59%), Gaps = 13/321 (4%) Query: 13 IQDQLKRPIRDLRLSLTDRCNFRCSYCMPKEIFGEDYAFLPARELLSFAEILRLTRLFVS 72 ++D+ +R + DLRLS+ D+CN RC YCMP+E Y +LP ++LLS EI + +F+S Sbjct: 18 VKDRCRRMMGDLRLSVIDQCNLRCRYCMPEE----HYTWLPRQDLLSVKEISAIVDVFLS 73 Query: 73 LGVVKIRLTGGEPLLRKDLPELV----AGIRSLNGVEDIGLTTNGVLLGQQAKGLYAAGL 128 +GV K+R+TGGEPL+R DLPE+V A + +G+ D+ +TTNGVLL + GL AAG+ Sbjct: 74 VGVSKVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGM 133 Query: 129 RRLNISLDALHPEIFGTMNGRGLKPSLILKHIDQAREMGY-EIKVNMVVQKGVNESEILP 187 +R+ +SLD L PE F ++ R +I I G+ + K++ V +G N E+ Sbjct: 134 KRITVSLDTLQPERFKAISQRNSHDKVI-AGIKAVAAAGFTDTKIDTTVMRGANHDELAD 192 Query: 188 MAAYFKERKITLRFIEFMDAGNDNGWSLAKVVTKKEILQSLREVY-ELEALEQDFYGEVA 246 + + + +RFIE+MD G W+ KV TK +L+SL + Y +E L + A Sbjct: 193 LIEFARTVNAEVRFIEYMDVGGATHWAWEKVFTKANMLESLEKRYGRIEPLPKHDTAP-A 251 Query: 247 QRYRYKDSDAEIGFITSVSESFCSSCTRARLSSDGKFYTCLFASGGTDLRSLLRSGADDS 306 RY D G I S +E FC++C R+RL++DG + CL+A G +LR LR+GA Sbjct: 252 NRYALPDG-TTFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGINLREPLRAGATHD 310 Query: 307 SLLNVIKDVWEKRTDRYSDER 327 L+ + W +RTDR +++R Sbjct: 311 DLVETVTTGWRRRTDRGAEQR 331 >MT3427 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 395 Score = 235 bits (599), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 125/325 (38%), Positives = 198/325 (60%), Gaps = 11/325 (3%) Query: 8 MNTTSIQDQLKRPIRDLRLSLTDRCNFRCSYCMPKEIFGEDYAFLPARELLSFAEILRLT 67 +N + I+D+ R + DLRLS+ D+CN RC YCMP+ +YA+LP +LLS EI + Sbjct: 49 INESPIRDRCGRTMGDLRLSVIDQCNLRCRYCMPEA----EYAWLPRADLLSVDEISLIV 104 Query: 68 RLFVSLGVVKIRLTGGEPLLRKDLPELV----AGIRSLNGVEDIGLTTNGVLLGQQAKGL 123 F+++GV KIRLTGGEPL+R DL ++ A + +G++D+ +TTNGVLL QA+ L Sbjct: 105 DAFIAVGVDKIRLTGGEPLIRSDLAAIIEVISAKVGDGSGLQDLAITTNGVLLADQARKL 164 Query: 124 YAAGLRRLNISLDALHPEIFGTMNGRGLKPSLILKHIDQAREMGY-EIKVNMVVQKGVNE 182 +AG+RR+ ISLD L P+ F ++ RG +I + I+ G+ + K++ VV +G N+ Sbjct: 165 KSAGMRRITISLDTLRPDRFKAISQRGTHYKVI-EGIEAVAAAGFTDTKLDSVVIRGFND 223 Query: 183 SEILPMAAYFKERKITLRFIEFMDAGNDNGWSLAKVVTKKEILQSLREVYELEALEQDFY 242 E+ + + + +RFIE+MD G WS+ KV TK ++L +L + Y A + Sbjct: 224 DELSDLIEFARNVNAEVRFIEYMDVGGATQWSMDKVFTKAQMLSTLGKKYGPIAALPKYD 283 Query: 243 GEVAQRYRYKDSDAEIGFITSVSESFCSSCTRARLSSDGKFYTCLFASGGTDLRSLLRSG 302 A RYR D G I S +E FC++C R+RL++DG + CL+A G +LR +R+G Sbjct: 284 SAPANRYRLPDG-TTFGIIASTTEPFCATCDRSRLTADGIWLHCLYALSGINLRESVRAG 342 Query: 303 ADDSSLLNVIKDVWEKRTDRYSDER 327 A + ++ +++ W R +R +++R Sbjct: 343 ASANDVVQILQRGWRDRANRGAEQR 367 >PA1505 [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 331 Score = 195 bits (495), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 126/340 (37%), Positives = 183/340 (53%), Gaps = 11/340 (3%) Query: 8 MNTTSIQDQLKRPIRDLRLSLTDRCNFRCSYCMPKEIFGEDYAFLPARELLSFAEILRLT 67 M+ + + D R I LRLS+TDRC+FRC+YCM ED FLP ++LS E+ + Sbjct: 1 MSDSRLVDPFGRRITYLRLSVTDRCDFRCTYCM-----SEDMQFLPRDQVLSLEELYAVA 55 Query: 68 RLFVSLGVVKIRLTGGEPLLRKDLPELVAGIRSLNGVEDIGLTTNGVLLGQQAKGLYAAG 127 F+ LGV +IR+TGGEPL+RK + L+A + +ED+ +TTNG L ++A L AAG Sbjct: 56 DAFIGLGVRRIRITGGEPLVRKGITGLLARLGQRAELEDLAITTNGSQLRERAAELKAAG 115 Query: 128 LRRLNISLDALHPEIFGTMNGRGLKPSLILKHIDQAREMGYE-IKVNMVVQKGVNESEIL 186 +RRLN+SLD+L E F R + +L I ARE G+E IK+N VVQKG N+ EIL Sbjct: 116 VRRLNVSLDSLQRERFAAFT-RSDRLEQVLDGIQAAREAGFERIKLNCVVQKGRNDDEIL 174 Query: 187 PMAAYFKERKITLRFIEFMDAGNDNGWSLAKVVTKKEILQSLREVYELEALEQDFYGEVA 246 + Y ++ + FIE M G+ + + ++++ E G + Sbjct: 175 DLVEYALANQLDISFIEEMPLGSITSHRRELTLCTSDEVRAIIERRWPLTPSLARSGGPS 234 Query: 247 QRYRYKDSDAEIGFITSVSESFCSSCTRARLSSDGKFYTCLFASGGTDLRSLLRSGADDS 306 + Y+ D + IGFI+ S +FC C R R++++GK CL G DLR LLRS D Sbjct: 235 RYYQIGDEPSRIGFISPHSNNFCGDCNRVRVTAEGKLVLCLGHEGALDLRELLRSHPGDR 294 Query: 307 SLLN-VIKDVWEKRTDRYSDERQEHTAAGRKKISMSYIGG 345 L+ + + +R+ + QE R MS GG Sbjct: 295 ERLSAALVAALNLKPERHHFDAQEQVQVLR---FMSMTGG 331 >Rv0869c [H] COG2896 Molybdenum cofactor biosynthesis enzyme Length = 360 Score = 180 bits (457), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 110/306 (35%), Positives = 156/306 (50%), Gaps = 11/306 (3%) Query: 9 NTTSIQDQLKRPIRDLRLSLTDRCNFRCSYCMPKEIFGEDYAFLPARELLSFAEILRLTR 68 T + D R DLR+SLTDRCN RCSYCMP+ +LP +LL E+ RL Sbjct: 26 TTGPLVDTFGRVANDLRVSLTDRCNLRCSYCMPER----GLRWLPGEQLLRPDELARLIH 81 Query: 69 LFVS-LGVVKIRLTGGEPLLRKDLPELVAGIRSLNGVEDIGLTTNGVLLGQQAKGLYAAG 127 + V+ LGV +R TGGEPLL L E+VA L +I LTTNGV L ++A L AG Sbjct: 82 IAVTRLGVTSVRFTGGEPLLAHHLDEVVAATARLRPRPEISLTTNGVGLARRAGALAEAG 141 Query: 128 LRRLNISLDALHPEIFGTMNGRGLKPSLILKHIDQAREMGYEIKVNMVVQKGVNESEILP 187 L R+N+SLD++ F + R ++ +KVN V+ +++ Sbjct: 142 LDRVNVSLDSIDRAHFAAITRRDRLAHVLAGLAAAKAAGLTPVKVNAVLDPTTGREDVVD 201 Query: 188 MAAYFKERKITLRFIEFMDAGNDNGWSLAKVVTKKEILQSLREVYELEALEQDFYGEVAQ 247 + + ER LR IE M + W ++ ++L +LR + L + G Sbjct: 202 LLRFCLERGYQLRVIEQMPLDAGHSWRRNIALSADDVLAALRPHFRLRP-DPAPRGSAPA 260 Query: 248 RYRYKDSDAE-----IGFITSVSESFCSSCTRARLSSDGKFYTCLFASGGTDLRSLLRSG 302 D+ G I SVS +FCS+C R RL++DG+ +CLF++ TDLR LLR G Sbjct: 261 ELWLVDAGPNTPRGRFGVIASVSHAFCSTCDRTRLTADGQIRSCLFSTEETDLRRLLRGG 320 Query: 303 ADDSSL 308 ADD ++ Sbjct: 321 ADDDAI 326 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,575,279 Number of extensions: 1021890 Number of successful extensions: 2106 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 2090 Number of HSP's successfully gapped: 29 Length of query: 345 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 238 Effective length of database: 26,037,693 Effective search space: 6196970934 Effective search space used: 6196970934 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)