BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0352.0004.p (484 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0437 [R] COG0790 FOG: TPR repeat, SEL1 subfamily 226 7e-59 CAC0355 [M] COG5434 Endopolygalacturonase 202 8e-52 AGl3361 [M] COG5434 Endopolygalacturonase 172 1e-42 RSp0833 [M] COG5434 Endopolygalacturonase 134 3e-31 CAC3684 [M] COG5434 Endopolygalacturonase 134 4e-31 >STM0437 [R] COG0790 FOG: TPR repeat, SEL1 subfamily Length = 673 Score = 226 bits (576), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 33/422 (7%), Positives = 70/422 (16%), Gaps = 45/422 (10%) Query: 16 PRIPGRTFVITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRI 75 G C + + + G + Sbjct: 21 LAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLM 80 Query: 76 NLSADQGALVQFDPDPALY-PLLSSQYEGNAGWRCQAPLDGEGLSDVAITGEGVFDGSGE 134 + L + + + A L + + Sbjct: 81 ISTFH--TLGLDIIKREYAALGMKANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLIST 138 Query: 135 G-----WRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKLADKGDTR 189 + + C L D Sbjct: 139 ISNWKNDLKTPSQAAAS----------AIGERDRIF-----------AHCYGLYDAHLKA 177 Query: 190 MEAFLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYS 249 L PTLL N + Q + + V + + Sbjct: 178 CNVLDFDDLILLPTLLLQANEEVRKRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSR--A 235 Query: 250 QNGDGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYH--G 307 + D + ++ ++ + + K + + I + Sbjct: 236 RFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNP 295 Query: 308 HGGVVIGSEMSGGVHAVRVSDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEA 367 H G ++V + V + + + H++ Sbjct: 296 HVFEKRLFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRGNHQS 355 Query: 368 VSFHMFYAGVEGSEGYDEQYFSVTEETPQFRKITLKNLVCHGAATALLVNGLPEMPLADL 427 F F L V P+ A L Sbjct: 356 RVFEKFLMQNRI------------PYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSAFL 403 Query: 428 TI 429 I Sbjct: 404 RI 405 >CAC0355 [M] COG5434 Endopolygalacturonase Length = 513 Score = 202 bits (515), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 132/476 (27%), Positives = 217/476 (45%), Gaps = 89/476 (18%) Query: 25 ITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRINLSADQGAL 84 I D+GA G + T+ IQAA+ C A G +V+IP G + TGPL L S I L ++G++ Sbjct: 86 ILDFGAVGDGNHVNTAFIQAAIQVCPAFG--RVIIPKGTYLTGPLFLKSNITLELEEGSV 143 Query: 85 VQFDPDPALYPLLSSQ------------YEGNAGWRCQAPLDGEGLSDVAITGEGVFDGS 132 + + YP+L + +EGN + + + +V I G+G DG+ Sbjct: 144 LLGLKEREHYPILKANISMTNRDFYLGSWEGNEADCFASLITAINVENVNIIGKGTIDGN 203 Query: 133 GEGWRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKLADKGDTRMEA 192 + W W+ ++E Sbjct: 204 SDF-----------DTW----------------WFKAKEK-------------------- 216 Query: 193 FLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYSQNG 252 R RP L L C+ +L++G T +NSP+W +HP + + N+ + NP+ + N Sbjct: 217 ----RIAWRPRTLFLNACKNILVEGVTIKNSPSWTIHPLMSDHLKFINLSIENPFNAPNT 272 Query: 253 DGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGHGGVV 312 D LD ESC + L+ +F VGDD I +KSGK + +K + I IRNC + GHG VV Sbjct: 273 DALDPESCKNVLILGDTFSVGDDCIAIKSGKIDISKKNPVSSENINIRNCNMRSGHGAVV 332 Query: 313 IGSEMSGGVHAVRVSDCLFIGTDIGLRFKSTRGRG--GVVEDIVMENISMTGIIHEAVSF 370 +GSEMS G+ ++ + C+F TD GLR K+ RGRG G++++I M+NI M ++ Sbjct: 333 LGSEMSSGLKSIFIEKCIFNATDRGLRIKTRRGRGSKGIIDNIHMKNIKMDKVLTPFSIN 392 Query: 371 HMFYAGVEGSEGY--DEQYFSVTEETPQFRKITLKNLVCHGA-ATALLVNGLPEMPLADL 427 ++ +G Y ++ V ++TP I ++++ C A A + GLPE + + Sbjct: 393 SFYFCDDDGKTEYVWSKEKLPVDDKTPYIGSIYVEDVTCTNAHVCAAFMYGLPEQKIEKV 452 Query: 428 TIEQ-------------------FRAVSERGIVLRHATGLTLDRIWLQTAETPEIT 464 +++ + G+ + L L+ + ++ A EIT Sbjct: 453 SMKNVSVSFDENAKEDYADMMSFLEPMKRNGMYFNNIKDLVLENVTVEKALNEEIT 508 >AGl3361 [M] COG5434 Endopolygalacturonase Length = 299 Score = 172 bits (435), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 0/428 (0%), Positives = 0/428 (0%), Gaps = 80/428 (18%) Query: 31 EEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRINLSADQGALVQFDPD 90 + C VV + + G L+ D Sbjct: 99 TMIPVAFESIVRPMLPSFCQR--YPDVVFEISTSERLDDIVTEGFDGGIRFGTLIDKDMV 156 Query: 91 PALYPLLSSQ---------YEGNAGWRCQAPLDGEGLSDVAITGEGVFDGSGEGWRPVKR 141 + + + ++ +F Sbjct: 157 ALPLTASTPIVIAGSASYFENYPIPLAPEHLAGHRCILYRYLSSSRLFRWPLA------- 209 Query: 142 FKMTEKQWNHLIESGGVLDPQGEVW-WPSREAMEGEGFCRKLADKGDTRMEAFLPARAYL 200 +G + Sbjct: 210 --------------------KGSQKFEY-KGDPAL--------------------IL--- 225 Query: 201 RPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYSQNGDGLDLESC 260 L G ++ A L ++ + + Sbjct: 226 DDGAAIRSAVMDGLGIGFLLRSQVASDLEAGNLREVLTDWLTDLPGFSLYYPSRRRTSPA 285 Query: 261 THALVEHSSFDVGDDAICLKSGKD-EEGRKRGLP-CRYITIRNCTVYHGHGGVVIGSEMS 318 Sbjct: 286 345 Query: 319 GGVHAVRVSDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEAVSFHMFYAGVE 378 Sbjct: 346 --- 402 Query: 379 GSEGYDEQYFS-----VTEETPQFRKITLKNL----VCHGAATALLVNGLPEMPLADLTI 429 Sbjct: 403 --- 459 Query: 430 EQFRAVSE 437 Sbjct: 460 467 >RSp0833 [M] COG5434 Endopolygalacturonase Length = 680 Score = 134 bits (337), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 110/420 (26%), Positives = 170/420 (40%), Gaps = 81/420 (19%) Query: 18 IPGRTFVITDYGAEEGGFT--------LCTSAIQAALDACAAMGG--------------- 54 P R FV+TDYGA+ S + A D A G Sbjct: 97 FPRRDFVVTDYGAQPCAIVDAVNPYTDASKSPLSAGADKTPATGAFDARAAFTAAIAACN 156 Query: 55 ----GKVVIPAGIWR-TGPLTLHSRINLSADQGALVQFDPDPALYP------------LL 97 G+V++PAG W GP+ L S +N + F P+P Y L Sbjct: 157 AAGGGRVLVPAGNWYCAGPIVLLSNVNFHLSADCTIYFSPNPDDYAKDGPVDCGANGKLY 216 Query: 98 SSQYEGNAGWRCQAPLDGEGLSDVAITGEG-----------VFDGSGEGWRPVKRFKMTE 146 S+++ N AP+ ++A+TGEG F GSG FK T+ Sbjct: 217 YSRWQSNDCLNYGAPVYARNQRNIALTGEGDSSTLNGQAMTPFAGSGNTSTCWWTFKGTK 276 Query: 147 KQWNHLIES-------------------GGVLDPQGEVWWPSREAMEGEGFCRKLADKGD 187 ++ + S G D + P+ + + + L++ G Sbjct: 277 GEYGAVNASTPSQAYSNPNNVDLRTAAPGIADDLYARLTDPTTPWQQDQNYLSALSEAGV 336 Query: 188 TRMEAFLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPW 247 + YLRP ++ C VL++ +P W HP C + +R V V + Sbjct: 337 AVAQRIFGKGHYLRPCMVEFIGCTNVLMEAYRTHATPCWQHHPTDCANVVIRGVTVDS-- 394 Query: 248 YSQNGDGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHG 307 N DG D ++C++ L E +F+ GDD I +KSGK+ + P + IRNC + G Sbjct: 395 IGPNNDGFDPDACSNVLCEDMTFNTGDDCIAIKSGKNLDTGYG--PAQDHVIRNCIMNSG 452 Query: 308 HGGVVIGSEMSGGVHAVRVSDCL----FIGTD---IGLRFKSTRGRGGVVEDIVMENISM 360 HGG+ +GSEM GGV + + F TD I +R K+ RGG V D ++++++ Sbjct: 453 HGGITLGSEMGGGVQRIYARNLTMRNAFYATDPLNIAIRIKTNMNRGGYVRDFYVDDVTL 512 >CAC3684 [M] COG5434 Endopolygalacturonase Length = 534 Score = 134 bits (336), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 123/426 (28%), Positives = 181/426 (42%), Gaps = 81/426 (19%) Query: 21 RTFVITDYGAEEGGFTLCTSAIQAALDACAAMGGGKVVIPAGIWRTGPLTLHSRINLSAD 80 + F +T YGA G TL T IQAA++AC GG VVIP G + +G + L S ++L D Sbjct: 127 QIFDVTKYGAVGDGKTLNTDKIQAAINACT--DGGTVVIPQGTFLSGAIYLKSNMSLRID 184 Query: 81 QGALVQFDP--DPAL----YPLLS-SQYEGNAGWRCQAPLDGEG-LSDVAITGEGVFDGS 132 G L+ D D A +P + S Y G A G +S+V I G G S Sbjct: 185 -GTLLGSDNVNDYAFTSKRFPYYTTSNYMG----LINAYTTNYGSISNVKIYGNGTV--S 237 Query: 133 GEGWRPVKRFKMTEKQWNHLIESGGVLDPQGEVWWPSREAMEGEGFCRKLADKGDTRMEA 192 G + K K+ + Q N KGD Sbjct: 238 GGTYTSGKLTKLGQAQTNL---------------------------------KGDKA--- 261 Query: 193 FLPARAYLRPTLLSLRNCRRVLLDGPTFQNSPAWCLHPFGCEQITVRNVHVRNPWYSQNG 252 R L++ + + L G N + + +TV + V+ + N Sbjct: 262 --------RGDLITAKGVTGLYLGGVNIVNPSEHSIFISYSKNVTVDGISVK-TYGIHNA 312 Query: 253 DGLDLESCTHALVEHSSFDVGDDAICLKSGKDEEGRKRGLPCRYITIRNCTVYHGHGGVV 312 DG+D+ + + A + +S FD GDD I L +G + K +P I I N GHGGVV Sbjct: 313 DGIDIANTSVANIFNSYFDNGDDCINLNAGYGADAVKENIPDNNIRIFNSNTGRGHGGVV 372 Query: 313 IGSEMSGGVHAVRVSDCLFIGTDIGLRFKSTRGRGGVVEDIVMENISMTGIIHEAVSFHM 372 GS + V V DC F GT+IGLRFK+++ GG +I + +++M I+++A+SF Sbjct: 373 FGSYTGAWIQNVSVEDCDFNGTNIGLRFKTSKEIGGGARNITVRDVTMENIVNDAISF-- 430 Query: 373 FYAGVEGSEGYDEQYFSVTEETPQ-----FRKITLKNLVCHGA-ATALLVNGLPEMPLAD 426 D Y + P F +TL N++C A + VN LP A+ Sbjct: 431 -----------DSNYGLTDVDNPAPVPGYFHDVTLSNIMCKKAGGYGIWVNALPSQYNAN 479 Query: 427 LTIEQF 432 L++ Sbjct: 480 LSLNNI 485 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.138 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,196,486 Number of extensions: 1706461 Number of successful extensions: 3330 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 3314 Number of HSP's successfully gapped: 10 Length of query: 484 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 374 Effective length of database: 25,691,691 Effective search space: 9608692434 Effective search space used: 9608692434 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)