BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0351.0011.p (216 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value kduD [IQR] COG1028 Dehydrogenases with different specificities (... 251 5e-67 BS_kduD [IQR] COG1028 Dehydrogenases with different specificitie... 251 5e-67 CAC0361 [IQR] COG1028 Dehydrogenases with different specificitie... 234 9e-62 YPO0839 [IQR] COG1028 Dehydrogenases with different specificitie... 232 4e-61 >kduD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 200 Score = 251 bits (641), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 0/207 (0%), Positives = 0/207 (0%), Gaps = 1/207 (0%) Query: 11 ETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMIRRTPAKDHSE 70 + +I L V + A + + A Sbjct: 47 IELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNALMQDL 106 Query: 71 KDWFDVINLNLNTVFLLSQIAGRHFLERGNG-KIINICSMLSYQGGINVPGYTASKHGVA 129 ++ ++ L + + ++ GY S G+ Sbjct: 107 EEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQLSWQGIE 166 Query: 130 GLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGRWGTAEDVKG 189 Sbjct: 167 226 Query: 190 PAVFLASAASDYLNGHILNVDGGWMAR 216 Sbjct: 227 253 >BS_kduD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 254 Score = 251 bits (641), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 18/207 (8%), Positives = 39/207 (18%), Gaps = 1/207 (0%) Query: 11 ETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMIRRTPAKDHSE 70 + + D +K + + D A I + K Sbjct: 47 SLSETQQLVEQEGRIFTSFTLDMSKPEAIKDSAAELFENRQIDILVNNAGIIHREKAEDF 106 Query: 71 KDWFDVINLNLNTVFLLSQIAGRHFLERGNG-KIINICSMLSYQGGINVPGYTASKHGVA 129 + LN+N L G I + L G + + Sbjct: 107 PEENWQHVLNVNLNSLFILTQLAGRHMLKRGHGKIINIASLLSFQGGILVPAYTASKHAV 166 Query: 130 GLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGRWGTAEDVKG 189 + + D + + + + D G Sbjct: 167 AGLTKSFANEWAASGIQVNAIAPGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIG 226 Query: 190 PAVFLASAASDYLNGHILNVDGGWMAR 216 ++ + + G Sbjct: 227 GTAVFLASRASDYVNGHILAVDGGWLS 253 Score = 247 bits (630), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 116/187 (62%), Positives = 148/187 (79%), Gaps = 2/187 (1%) Query: 32 DHTKLQGMFDEAVAF--TGKVDILVNCAGMIRRTPAKDHSEKDWFDVINLNLNTVFLLSQ 89 D +K + + D A ++DILVN AG+I R A+D E++W V+N+NLN++F+L+Q Sbjct: 68 DMSKPEAIKDSAAELFENRQIDILVNNAGIIHREKAEDFPEENWQHVLNVNLNSLFILTQ 127 Query: 90 IAGRHFLERGNGKIINICSMLSYQGGINVPGYTASKHGVAGLTKAFANEWAGSGLNINAI 149 +AGRH L+RG+GKIINI S+LS+QGGI VP YTASKH VAGLTK+FANEWA SG+ +NAI Sbjct: 128 LAGRHMLKRGHGKIINIASLLSFQGGILVPAYTASKHAVAGLTKSFANEWAASGIQVNAI 187 Query: 150 APGYMATENTAPIRADQNRADSILDRIPAGRWGTAEDVKGPAVFLASAASDYLNGHILNV 209 APGY++T NT PIR D+ R + IL RIPAGRWG A+D+ G AVFLAS ASDY+NGHIL V Sbjct: 188 APGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIGGTAVFLASRASDYVNGHILAV 247 Query: 210 DGGWMAR 216 DGGW++R Sbjct: 248 DGGWLSR 254 >CAC0361 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 260 Score = 234 bits (596), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 116/207 (56%), Positives = 146/207 (70%) Query: 10 DETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMIRRTPAKDHS 69 DET + G K DLS + G+FD+ + G++DILVN AG IRR P ++ Sbjct: 54 DETRKLIEDEGRKVEFYKADLSKKESIPGIFDKCMELYGRLDILVNNAGTIRRAPITEYR 113 Query: 70 EKDWFDVINLNLNTVFLLSQIAGRHFLERGNGKIINICSMLSYQGGINVPGYTASKHGVA 129 ++DW +IN NLN+++ LSQ A + +++G+GKIINI SMLS+QGG VP YTASKHGVA Sbjct: 114 DEDWEYIINTNLNSIYHLSQRAAKIMIKQGSGKIINIASMLSFQGGKFVPPYTASKHGVA 173 Query: 130 GLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAGRWGTAEDVKG 189 GLTKAFANE A + INAIAPGY+ T+NT IR D++R + IL RIPAG+WG DV G Sbjct: 174 GLTKAFANELADKNIQINAIAPGYIVTKNTEAIRNDESRNNEILSRIPAGKWGYPFDVMG 233 Query: 190 PAVFLASAASDYLNGHILNVDGGWMAR 216 VFLAS ASDY+NGHIL VDGGW+ R Sbjct: 234 TVVFLASRASDYINGHILAVDGGWLVR 260 >YPO0839 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 251 Score = 232 bits (591), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 114/217 (52%), Positives = 149/217 (68%), Gaps = 1/217 (0%) Query: 1 VISASLNASDETVAAAKAFGVKALSIAGDLSDHTKLQGMFDEAVAFTGKVDILVNCAGMI 60 ++ + + ET A +A G K I DL + + +AV G +DIL+N AG+I Sbjct: 35 IVGVGVAEAPETQAQVEALGRKFHFITADLIQQKDIDSIVSQAVEVMGHIDILINNAGII 94 Query: 61 RRTPAKDHSEKDWFDVINLNLNTVFLLSQIAGRHFLERGNG-KIINICSMLSYQGGINVP 119 RR + KDW DVIN+N TVF LSQ + F+++GNG KIINI SMLS+QGGI VP Sbjct: 95 RRQDLLEFGNKDWDDVININQKTVFFLSQAVAKQFVKQGNGGKIINIASMLSFQGGIRVP 154 Query: 120 GYTASKHGVAGLTKAFANEWAGSGLNINAIAPGYMATENTAPIRADQNRADSILDRIPAG 179 YTASK V GLT+A A E + +N+NAIAPGYMAT+NTA +RAD R ++IL+RIPA Sbjct: 155 SYTASKSAVMGLTRALATELSQYNINVNAIAPGYMATDNTAALRADTARNEAILERIPAS 214 Query: 180 RWGTAEDVKGPAVFLASAASDYLNGHILNVDGGWMAR 216 RWGT +D+ GPA+FL+S+ASDY+ G+ L VDGGW+AR Sbjct: 215 RWGTPDDLAGPAIFLSSSASDYVTGYTLAVDGGWLAR 251 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,021,518 Number of extensions: 646107 Number of successful extensions: 1754 Number of sequences better than 1.0e-05: 271 Number of HSP's gapped: 1481 Number of HSP's successfully gapped: 271 Length of query: 216 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 114 Effective length of database: 26,614,363 Effective search space: 3034037382 Effective search space used: 3034037382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 113 (48.1 bits)