BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0066.p (185 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1763 [C] COG1140 Nitrate reductase beta subunit 152 3e-37 CPn0895 [J] COG0231 Translation elongation factor P (EF-P)/trans... 143 2e-34 CT752 [J] COG0231 Translation elongation factor P (EF-P)/transla... 142 2e-34 VC2660 [J] COG0231 Translation elongation factor P (EF-P)/transl... 142 3e-34 XF2473 [J] COG0231 Translation elongation factor P (EF-P)/transl... 141 6e-34 >STM1763 [C] COG1140 Nitrate reductase beta subunit Length = 346 Score = 152 bits (384), Expect = 3e-37, Method: Compositional matrix adjust. Identities = 17/185 (9%), Positives = 42/185 (22%) Query: 1 VISVNDFKTGLTVEVDGDIFTVLDFQHVKPGKGAAFVRSKLKNLRNGNTVERTFRAGETI 60 + + L V G Q+ + A + + + + E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 GRAIIENRGVQYLYASGSDHVFMDNETYDQFELSAKQLEWELNFLKENMTVNIVSYQGEI 120 + + E +L GS + + + L + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 LGINLPTSVELKVTETEPGVKGNTAQGATKAATLETGLTVQVPLFINENDVLLIDTREGK 180 L + ++ + G T +A + L L + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 181 YISRA 185 + Sbjct: 181 ETAFG 185 >CPn0895 [J] COG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A) Length = 190 Score = 143 bits (360), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 72/184 (39%), Positives = 113/184 (61%), Gaps = 1/184 (0%) Query: 2 ISVNDFKTGLTVEVDGDIFTVLDFQHVKPGKGAAFVRSKLKNLRNGNTVERTFRAGETIG 61 +S ++F+ GL +E+DG + +L VKPGKG AF R K+KN G +ERT+++GE++ Sbjct: 4 VSTSEFRVGLRIEIDGQPYLILQNDFVKPGKGQAFNRIKVKNFLTGRVIERTYKSGESVE 63 Query: 62 RAIIENRGVQYLYASGSDHVFMDNETYDQFELSAKQLEWELNFLKENMTVNIVSYQGEIL 121 A I R ++ LY FMD+ET++Q + ++LE +L E+ +V Y G+++ Sbjct: 64 TADIVERSMRLLYTDQEGATFMDDETFEQEVVFWEKLENIRQWLLEDTIYTLVLYNGDVV 123 Query: 122 GINLPTSVELKVTETEPGVKGNTAQG-ATKAATLETGLTVQVPLFINENDVLLIDTREGK 180 + P +EL + ET PGV+G+TA G K A TG + VP+FI+E +++ +DTR G Sbjct: 124 AVEPPIFMELSIAETAPGVRGDTASGRVLKPAVTNTGAKIMVPIFIDEGELVKVDTRTGS 183 Query: 181 YISR 184 Y SR Sbjct: 184 YESR 187 >CT752 [J] COG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A) Length = 190 Score = 142 bits (359), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 73/184 (39%), Positives = 112/184 (60%), Gaps = 1/184 (0%) Query: 2 ISVNDFKTGLTVEVDGDIFTVLDFQHVKPGKGAAFVRSKLKNLRNGNTVERTFRAGETIG 61 +S ++F+ GL V++DG + +L VKPGKG AF R K+KN G +E+TF++GE+I Sbjct: 4 VSTSEFRVGLRVKIDGQPYVILQNDFVKPGKGQAFNRIKVKNFLTGRVIEKTFKSGESIE 63 Query: 62 RAIIENRGVQYLYASGSDHVFMDNETYDQFELSAKQLEWELNFLKENMTVNIVSYQGEIL 121 A + + ++ LY FMD+ET++Q + +LE +L E+ +V Y G+++ Sbjct: 64 TADVREQQMRLLYTDQEGATFMDDETFEQELIFWDKLENVRQWLLEDTIYTLVLYNGDVI 123 Query: 122 GINLPTSVELKVTETEPGVKGNTAQG-ATKAATLETGLTVQVPLFINENDVLLIDTREGK 180 + P +EL + ET PGV+G+TA G K AT TG + VP+FI E +V+ +DTR G Sbjct: 124 SVEPPIFMELTIAETAPGVRGDTASGRVLKPATTNTGAKIMVPIFIEEGEVVKVDTRTGS 183 Query: 181 YISR 184 Y SR Sbjct: 184 YESR 187 >VC2660 [J] COG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A) Length = 188 Score = 142 bits (358), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 72/183 (39%), Positives = 112/183 (61%) Query: 2 ISVNDFKTGLTVEVDGDIFTVLDFQHVKPGKGAAFVRSKLKNLRNGNTVERTFRAGETIG 61 +S N+FK GL + +D + +L+ ++VKPGKG AF R +++ L G +E+TF++G+T Sbjct: 4 VSTNEFKGGLKIMLDNEPCVILENEYVKPGKGQAFNRVRIRKLLTGKVLEKTFKSGDTAE 63 Query: 62 RAIIENRGVQYLYASGSDHVFMDNETYDQFELSAKQLEWELNFLKENMTVNIVSYQGEIL 121 A + + + YLY G + FM+N T++Q AK + +L EN T + + G + Sbjct: 64 VADVVDIDLDYLYNDGEFYHFMNNSTFEQLAADAKAVGENAKWLVENNTCMLTLWNGNPI 123 Query: 122 GINLPTSVELKVTETEPGVKGNTAQGATKAATLETGLTVQVPLFINENDVLLIDTREGKY 181 + P VEL+VTET+PGVKG+T K ATL TG V+VPLF+ +V+ +DTR +Y Sbjct: 124 AVTPPNFVELEVTETDPGVKGDTQGTGGKPATLSTGAVVRVPLFVQIGEVIKVDTRSAEY 183 Query: 182 ISR 184 + R Sbjct: 184 VGR 186 >XF2473 [J] COG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A) Length = 193 Score = 141 bits (355), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 69/181 (38%), Positives = 113/181 (62%) Query: 4 VNDFKTGLTVEVDGDIFTVLDFQHVKPGKGAAFVRSKLKNLRNGNTVERTFRAGETIGRA 63 +ND K G+ + V+ + + D ++VKPGKG AF R K + +++G E T ++ +T+ A Sbjct: 11 MNDVKNGMKILVNAEPAVITDTEYVKPGKGQAFTRVKYRLIKSGRVQEVTMKSTDTLEAA 70 Query: 64 IIENRGVQYLYASGSDHVFMDNETYDQFELSAKQLEWELNFLKENMTVNIVSYQGEILGI 123 + + +QYLY+ G FM ET++Q + + +LK + + G +G+ Sbjct: 71 DVVDTDMQYLYSDGEYWHFMQQETFEQVQADKNGMGGAEKWLKGEEQCVVTLWNGVPIGV 130 Query: 124 NLPTSVELKVTETEPGVKGNTAQGATKAATLETGLTVQVPLFINENDVLLIDTREGKYIS 183 P VELK+TET+PG++G+T+ G K ATLETG V+VPLF+N+++V+ +DTR G+Y+S Sbjct: 131 QPPNFVELKITETDPGLRGDTSGGGGKPATLETGAVVRVPLFVNQDEVIKVDTRSGEYVS 190 Query: 184 R 184 R Sbjct: 191 R 191 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 13,012,427 Number of extensions: 492750 Number of successful extensions: 996 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 986 Number of HSP's successfully gapped: 23 Length of query: 185 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 85 Effective length of database: 26,845,031 Effective search space: 2281827635 Effective search space used: 2281827635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 112 (47.8 bits)