BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0061.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2805 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 233 2e-61 BS_yqhO [R] COG1752 Predicted esterase of the alpha-beta hydrola... 210 2e-54 Rv2037c [R] COG1752 Predicted esterase of the alpha-beta hydrola... 174 2e-43 MT2097 [R] COG1752 Predicted esterase of the alpha-beta hydrolas... 174 2e-43 RSc1178 [R] COG1752 Predicted esterase of the alpha-beta hydrola... 62 1e-09 >BH2805 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Length = 296 Score = 233 bits (595), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 128/307 (41%), Positives = 176/307 (57%), Gaps = 13/307 (4%) Query: 1 MEINAVFEGGGVKGISLAGAVQASEQAGAVFKRVAGTSSGSIIASLLAVGYDGEAMSRII 60 M ++ VF GGGVK + GA+Q E+ G F RVAGTS+G+I ASL+ GY + + I+ Sbjct: 1 MNVDGVFAGGGVKAFAFIGALQVLEENGFSFTRVAGTSAGAIFASLVCSGYRSDELFSIM 60 Query: 61 RTTAFTSFLKRGFLYNTAYVGPAFRVLIKKGLYSGEALESWIRGILKEKGLVTFGDLPRG 120 F G + + R+ K G+Y G+ LE W+ +L KG+ TF DLP+G Sbjct: 61 EQLDGKMFKDGGKVMPFPF-AKWLRLYFKLGMYKGDQLEKWLIQVLGHKGVSTFADLPKG 119 Query: 121 KLSIIASDITNGRLVVLPDDLKQYGISPDGFEVAKAVRMSCSIPYFFDPVMLRLSNEAAK 180 L I+ASDI+ GRL+VLPDDLK YG+ P+ F VA+AVRMSC IPYFF PV +L N + Sbjct: 120 ALKIVASDISRGRLIVLPDDLKDYGLIPERFSVARAVRMSCGIPYFFQPV--KLYNRKRR 177 Query: 181 GKSFAEQFVYMVDGGLLSNFPLWLFDEKEEGFKSPGRRIPTVGYQMI-GKTEPQPHRITG 239 G+ Y+VDGG+LSNFP+WLF ++ K P +R P +G+Q+ G E PH I Sbjct: 178 GER-----SYVVDGGILSNFPIWLFHNRK---KQPLKR-PVIGFQLTPGLEEMAPHLINN 228 Query: 240 PFSMLQAMVGTMLSAHDERYIETEKFIRTVKIPTLGIAMTEFNLAPERSDELYAAGYKAG 299 + Q++ TM+ AHD RYI + V IP + T+F+L + L G + Sbjct: 229 AVDLYQSLFDTMIRAHDLRYIAKDHAKNIVFIPVQSVKATDFDLDEAQKKALVQLGREET 288 Query: 300 EAFFKNW 306 F W Sbjct: 289 LQFLSTW 295 >BS_yqhO [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Length = 291 Score = 210 bits (535), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 122/307 (39%), Positives = 177/307 (57%), Gaps = 19/307 (6%) Query: 1 MEINAVFEGGGVKGISLAGAVQASEQAGAVFKRVAGTSSGSIIASLLAVGYDGEAMSRII 60 M I+ VF GGGVKGI+LAGA + E+ G FKRVAGTS+G+IIA+ +A GY + + +I Sbjct: 1 MYIDGVFSGGGVKGIALAGAYEVLEEKGFRFKRVAGTSAGAIIAAFIASGYTSKEIHALI 60 Query: 61 RTTAFTSFLKRGFLYNTAYVGPAFRVLIKKGLYSGEALESWIRGILKEKGLVTFGDLPRG 120 L + + + + + + GLY G+ +E WI +L+ KG+ FGDL +G Sbjct: 61 EEVDGEKLLDQRYSFLPLKMLQWVSIYWRLGLYKGDTIEKWIADLLRAKGIRVFGDLQKG 120 Query: 121 KLSIIASDITNGRLVVLPDDLKQYGISPDGFEVAKAVRMSCSIPYFFDPVMLRLSNEAAK 180 L +IASD+TNG ++VLPDDL +YG++PD F VA+AVRMSCSIPYFF+P+ L+ A Sbjct: 121 SLRLIASDLTNGTMIVLPDDLARYGLNPDMFPVARAVRMSCSIPYFFEPIKLKTDTGTAT 180 Query: 181 GKSFAEQFVYMVDGGLLSNFPLWLFDEKEEGFKSPGRRIPTVGYQMIGKTEPQPHR-ITG 239 +VDGG+LSNFP+WLF S R+ P +G + + +P + I Sbjct: 181 ----------VVDGGVLSNFPIWLF--------SKERKRPVIGVTLAPRERERPKKNIRN 222 Query: 240 PFSMLQAMVGTMLSAHDERYIETEKFIRTVKIPTLGIAMTEFNLAPERSDELYAAGYKAG 299 F + A+ TM AHD R+I + + +P + T+F+L ++ L G + Sbjct: 223 AFELFGALFETMKDAHDARHIASRYEQNIIFLPVDDVMATDFHLTQQKKLALIELGKRRT 282 Query: 300 EAFFKNW 306 E F K W Sbjct: 283 ELFLKQW 289 >Rv2037c [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Length = 324 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 120/319 (37%), Positives = 181/319 (56%), Gaps = 33/319 (10%) Query: 2 EINAVFEGGGVKGISLAGAVQASEQAGAVFKRVAGTSSGSIIASLLA-VGYDGEAMSRI- 59 ++ V EGGGV+GI L GAV A AG F RVAG+S+G+I+ASL+A + GE ++R+ Sbjct: 8 RVDLVCEGGGVRGIGLVGAVDALADAGYRFPRVAGSSAGAIVASLVAALQTAGEPVTRLA 67 Query: 60 --IRTTAFTSFLKRGFLYNTAYVGPAFRVLIKKGLYSGEALESWIRGILKEKGLVTFGDL 117 +R+ + FL R + + +G +L+ G+Y G LE + G+L + G+ TFGDL Sbjct: 68 EMMRSIDYPKFLDRNLIGHVPLIGGGLSLLLSDGVYRGAYLEQLLGGLLADLGVHTFGDL 127 Query: 118 PRGK--------LSIIASDITNGRLVVLPDDLKQYGISPDGFEVAKAVRMSCSIPYFFDP 169 G+ L + ASD++ RLV +P DL YGI PD F VA+AV S +IP+ F+P Sbjct: 128 RTGEAPEQFAWSLVVTASDLSRRRLVRIPWDLDSYGIHPDDFSVARAVHASSAIPFVFEP 187 Query: 170 VMLRLSNEAAKGKSFAEQFVYMVDGGLLSNFPLWLFDEKEEGFKSPGRRIPTVGYQMIGK 229 V +R G ++ VDGGLLSNFP+ LFD + P R PT G ++ + Sbjct: 188 VRVR-------GATW-------VDGGLLSNFPVALFDRTD---AEP--RWPTFGIRLSAR 228 Query: 230 TEPQPHR-ITGPFSMLQAMVGTMLSAHDERYIETEKFI-RTVKIPTLGIAMTEFNLAPER 287 P R + GP S+ A + T++S D YI+ + RT+ +P ++ +F++ E+ Sbjct: 229 PGIPPTRPVQGPVSLGIAAIETLVSNQDNAYIDDPCTVRRTIFVPAHDVSPIDFDITAEQ 288 Query: 288 SDELYAAGYKAGEAFFKNW 306 + LY G++AG+ F NW Sbjct: 289 REALYQRGFQAGQKFLANW 307 >MT2097 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Length = 324 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 120/319 (37%), Positives = 181/319 (56%), Gaps = 33/319 (10%) Query: 2 EINAVFEGGGVKGISLAGAVQASEQAGAVFKRVAGTSSGSIIASLLA-VGYDGEAMSRI- 59 ++ V EGGGV+GI L GAV A AG F RVAG+S+G+I+ASL+A + GE ++R+ Sbjct: 8 RVDLVCEGGGVRGIGLVGAVDALADAGYRFPRVAGSSAGAIVASLVAALQTAGEPVTRLA 67 Query: 60 --IRTTAFTSFLKRGFLYNTAYVGPAFRVLIKKGLYSGEALESWIRGILKEKGLVTFGDL 117 +R+ + FL R + + +G +L+ G+Y G LE + G+L + G+ TFGDL Sbjct: 68 EMMRSIDYPKFLDRNLIGHVPLIGGGLSLLLSDGVYRGAYLEQLLGGLLADLGVHTFGDL 127 Query: 118 PRGK--------LSIIASDITNGRLVVLPDDLKQYGISPDGFEVAKAVRMSCSIPYFFDP 169 G+ L + ASD++ RLV +P DL YGI PD F VA+AV S +IP+ F+P Sbjct: 128 RTGEAPEQFAWSLVVTASDLSRRRLVRIPWDLDSYGIHPDDFSVARAVHASSAIPFVFEP 187 Query: 170 VMLRLSNEAAKGKSFAEQFVYMVDGGLLSNFPLWLFDEKEEGFKSPGRRIPTVGYQMIGK 229 V +R G ++ VDGGLLSNFP+ LFD + P R PT G ++ + Sbjct: 188 VRVR-------GATW-------VDGGLLSNFPVALFDRTD---AEP--RWPTFGIRLSAR 228 Query: 230 TEPQPHR-ITGPFSMLQAMVGTMLSAHDERYIETEKFI-RTVKIPTLGIAMTEFNLAPER 287 P R + GP S+ A + T++S D YI+ + RT+ +P ++ +F++ E+ Sbjct: 229 PGIPPTRPVQGPVSLGIAAIETLVSNQDNAYIDDPCTVRRTIFVPAHDVSPIDFDITAEQ 288 Query: 288 SDELYAAGYKAGEAFFKNW 306 + LY G++AG+ F NW Sbjct: 289 REALYQRGFQAGQKFLANW 307 >RSc1178 [R] COG1752 Predicted esterase of the alpha-beta hydrolase superfamily Length = 319 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 53/202 (26%), Positives = 86/202 (42%), Gaps = 39/202 (19%) Query: 1 MEINAVFEGGGVKGISLAGAVQASEQAGAVFKRVAGTSSGSIIASLLAVGYDGEAMSRII 60 + I GG +G + G ++A E G + GTS+GS++A+L A G G ++R Sbjct: 51 VRIGLALGGGAARGFAHIGVIKALEAQGIQIDFITGTSAGSVVAALYASGMSGIELNRQA 110 Query: 61 RTTAFTSFLKRGFLYNTAYVGPAFRVLIKKGLYSGEALESWIRGILKEKGLVTFGDLPRG 120 + + T + G GEALE ++ ++K+K + R Sbjct: 111 LKMDEATIADWALPFGTRF----------GGWLKGEALEKYVNRLVKQKTIEQM----RI 156 Query: 121 KLSIIASDITNGRLVVLPDDLKQYGISPDGFEVAKAVRMSCSIPYFFDPVMLRLSNEAAK 180 L I+A+D+ +GR ++ +AVR SCSIP F PV + Sbjct: 157 PLGIVATDLGSGRPILFRRG-----------NTGQAVRASCSIPGVFQPVTI-------S 198 Query: 181 GKSFAEQFVYMVDGGLLSNFPL 202 G + VDGGL++ P+ Sbjct: 199 GHQY-------VDGGLVAPVPV 213 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,605,198 Number of extensions: 979040 Number of successful extensions: 2342 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2322 Number of HSP's successfully gapped: 8 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)