BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0060.p (331 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1776_2 [S] COG1649 Uncharacterized protein conserved in bacteria 236 4e-62 all2116 [S] COG1649 Uncharacterized protein conserved in bacteria 87 3e-17 all4081 [S] COG1649 Uncharacterized protein conserved in bacteria 84 3e-16 all1210_2 [S] COG1649 Uncharacterized protein conserved in bacteria 84 3e-16 DR2412 [S] COG1649 Uncharacterized protein conserved in bacteria 73 7e-13 >all1776_2 [S] COG1649 Uncharacterized protein conserved in bacteria Length = 455 Score = 236 bits (602), Expect = 4e-62, Method: Compositional matrix adjust. Identities = 126/323 (39%), Positives = 183/323 (56%), Gaps = 19/323 (5%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGK--NPGYDPLEFMISAAHS 58 +L +LQ+ FNAV +QVRP GD+LY S L PWS LTG+ GK P YDPL+F I+ AH Sbjct: 35 ILTRLQQLNFNAVILQVRPEGDALYASELEPWSAWLTGTPGKAPEPFYDPLQFAIAEAHK 94 Query: 59 RGMQFHAWFNPFRATTDANTANLAGNHVAKAHPEWIVKADSKLYINPGIPEARQHIIDTV 118 R ++ HAWFNP+RA T +A H++ +PE + + ++L+++PGI + + + Sbjct: 95 RNLEVHAWFNPYRAKTSTRSAPNVRPHISVTNPEVVYQWGNQLWMDPGIKIVQDRAYNVI 154 Query: 119 MEVVKGYDIDGVHLDDYFYP----SGSFADDEAFKTYNSKSIS-SKGDWRRDNINDFISR 173 ++VV+ YDID VHLDDYFYP +F DD+ + Y S S DWRR N++ + R Sbjct: 155 IDVVRRYDIDAVHLDDYFYPYPIQGQAFPDDKTYAAYKSAGGQLSLNDWRRQNVDQMVLR 214 Query: 174 LGQSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYADTRTWIRSGWIDYI 233 L Q I KS V FG+SPFG++R + G+ AY +YAD + W+ GW+DY+ Sbjct: 215 LSQGIKATKSYVKFGISPFGIYRPGQPPG-----ITGLDAYSVLYADAKKWLEQGWVDYL 269 Query: 234 APQIYWSLSFNAARYDKLVDWWVNEVKGSGVKLYIGQATYKVGTDKSAEWQSSEQIINQL 293 APQ+YW Y L+ WW E+ +Y G +G W+ +E+I Q+ Sbjct: 270 APQLYWRTDQTNQSYPVLLKWWT-EINSKRRHIYAGN---NIGQLDGKAWK-NEEIEKQV 324 Query: 294 KY--NEKYNEVAGSIMFRANDIV 314 K N+ G+I F + I+ Sbjct: 325 KISRNQAGELSLGNIFFSVSSII 347 >all2116 [S] COG1649 Uncharacterized protein conserved in bacteria Length = 416 Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 75/263 (28%), Positives = 115/263 (43%), Gaps = 52/263 (19%) Query: 2 LDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGY---DPLEFMISAAHS 58 L KL K FN V+ V G +LYPS + +KV+ + PG D L+ +++ H Sbjct: 80 LQKLDKLNFNTVYPAVWNWGYTLYPSKVA--AKVIGRAIDPTPGLQGRDMLKEIVTEGHK 137 Query: 59 RGMQFHAWFN-PFRATTDANTANLAGNHVAKAHPEWIVKADS-----------KLYINPG 106 +G+ WF F A D+ +AK P+W+ + ++++NP Sbjct: 138 QGLTVIPWFEFGFMAPADS--------LLAKNRPQWLTSRSNGSRIVKEGIHDRVWLNPF 189 Query: 107 IPEARQHIIDTVMEVVKGYDIDGVHLDDYF-YPSGSFADDEAFKTYNSK---SISSKGD- 161 P+ +Q I D ++E+V+ YDIDG+ DD+F PS D Y + SK Sbjct: 190 RPDVQQFIQDLIVEIVRNYDIDGIQFDDHFGLPSELGYDAYTVALYKKEHRGQAPSKNPR 249 Query: 162 ------WRRDNINDFISRLGQSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYD 215 WR I +F+ R+ ++I K D V+P N + S YD Sbjct: 250 DPEWLRWRASKITNFMQRIFKAIKATKKDCLVSVAP-----NPQRFS-----------YD 293 Query: 216 DMYADTRTWIRSGWIDYIAPQIY 238 AD + W R G I+ + QIY Sbjct: 294 YFLADWQKWERMGLIEELVLQIY 316 >all4081 [S] COG1649 Uncharacterized protein conserved in bacteria Length = 430 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 70/259 (27%), Positives = 109/259 (42%), Gaps = 46/259 (17%) Query: 2 LDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRGM 61 + KL++ FN V+ V SG +YPS + + + G+D + +I+ AHSRG+ Sbjct: 102 MSKLRQLNFNTVYPVVWNSGYVMYPSAVAQRYGIQPFVFRGSDGHDIVADVINQAHSRGL 161 Query: 62 QFHAWFN-PFRATTDANTANLAGNHVAKAHPEWIVK-----------ADSKLYINPGIPE 109 WF F A T+ LA NH PEW+ K A +++NP P+ Sbjct: 162 LAIPWFEFGFMAPP---TSELAMNH-----PEWLTKKRNGGQTSISAAGEVVWLNPFHPQ 213 Query: 110 ARQHIIDTVMEVVKGYDIDGVHLDDYFYPSGSFADDEAFKTYNSKSISSKGD-------- 161 ++ I D V+E+V YD+DG+ DD+ F D+ + +K + Sbjct: 214 VQRLITDLVLEIVTNYDVDGIQFDDHMSLPHEFGYDKYTVSLYTKETKNPPPTNHQDPAW 273 Query: 162 --WRRDNINDFISRLGQSIHGVKSDVSFGVSPFGVWRNKKADSTGSDTTAGVSAYDDMYA 219 WR D I F+ +L Q++ K + F V+P AY Sbjct: 274 VRWRSDKITAFMVKLNQAVKARKPKIIFSVAP----------------NYYDFAYKFQLQ 317 Query: 220 DTRTWIRSGWIDYIAPQIY 238 D WIR +D + Q+Y Sbjct: 318 DWLAWIRLKIVDELIVQVY 336 >all1210_2 [S] COG1649 Uncharacterized protein conserved in bacteria Length = 489 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 71/307 (23%), Positives = 120/307 (39%), Gaps = 64/307 (20%) Query: 1 LLDKLQKTGFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRG 60 + D+L + G N +F + +G ++YPS + P L G+DPL + AH+RG Sbjct: 27 IFDRLAQAGINTIFFETINAGYTIYPSKVAPQQNPLIR------GWDPLASGVKLAHARG 80 Query: 61 MQFHAWFNPFRATTDANTA--NLAGNH---VAKAHPEW---------IVKADSKLYINPG 106 M+ HAW F A + N+ N+ V A+P+W I +K + +P Sbjct: 81 MELHAWVWTFAAGNQRHNELLNIPTNYPGPVLAANPDWANYDHQGQMIPLGQTKPFFDPA 140 Query: 107 IPEARQHIIDTVMEVVKGYDIDGVHLDDYFYPSGSFADDEAFKTYN-------------- 152 PE RQ+++ E++ Y +DG+ LD YP F D A ++Y Sbjct: 141 NPELRQYLLKLYEEIITKYKVDGLQLDYIRYP---FQDPAAGRSYGYGKAARTQFQQLTG 197 Query: 153 ----SKSISSKGDW------RRDNINDFISRLGQSIHGVKSDVSFGVSPFGVWRNKKADS 202 S S W R ++ F++++ Q + ++ V+ F + ++ Sbjct: 198 VDPMKISPSQTQLWQQWTTFRTQQVDSFVAQVSQMLRQQDRNLILSVAVFPLPEYERVQK 257 Query: 203 TGSDTTAGVSAYDDMYADTRTWIRSGWIDYIAPQIYWSLSFNAARYDKLVDWWVNEVKGS 262 + W R G ID I P Y + + R+ L W+ + Sbjct: 258 --------------IQQHWEIWARQGNIDLIIPMTY---AQDTVRFQTLAKPWITSTQLG 300 Query: 263 GVKLYIG 269 L G Sbjct: 301 STLLIPG 307 >DR2412 [S] COG1649 Uncharacterized protein conserved in bacteria Length = 579 Score = 72.8 bits (177), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 63/263 (23%), Positives = 107/263 (40%), Gaps = 49/263 (18%) Query: 9 GFNAVFVQVRPSGDSLYPSVLVPWSKVLTGSQGKNPGYDPLEFMISAAHSRGMQFHAWFN 68 G N +FVQ GD L +P L Q +DPL + AH RG++ AW + Sbjct: 91 GVNTLFVQAIRRGDCLCMKSGLP----LITDQALEKNFDPLAIVTKLAHERGIKVIAWAS 146 Query: 69 PFRATTDANTANLAGNHVAKAH-------------PE--WIVKADSKLYINPGIPEARQH 113 +A + A HV K H P+ W+ D +++ GIP+A + Sbjct: 147 -VTGIANAAVPSTAPGHVMKTHGPSSGAQSWLARRPDGSWLEGKDG--WLDAGIPDAAEF 203 Query: 114 IIDTVMEVVKGYDIDGVHLDDYFYPSGS--FADDEAFKTYNSKSISSKGD---------W 162 + +V+ +V+ Y +DG+ LD YP G D + Y +++ ++ W Sbjct: 204 MAQSVVNLVRNYRVDGIQLDRIRYPDGGDWGYDPKTLTRYRAETGAAGTPAASDPRWQAW 263 Query: 163 RRDNINDFISRLGQSIHGVKSD-------VSFGVSPFGVWRNKKADSTGSDTTAGVSAYD 215 +R+ I + R+ + V+ D +++G P K D + V+ Sbjct: 264 KREQITALVRRIALEVKSVRPDAWLSAATITYGAPP------KPGDLAAFTRSRTVT--- 314 Query: 216 DMYADTRTWIRSGWIDYIAPQIY 238 D+ + W+R G I+ P Y Sbjct: 315 DVMQNWPEWVRDGLIELNVPMNY 337 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,951,368 Number of extensions: 1076955 Number of successful extensions: 1765 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1737 Number of HSP's successfully gapped: 10 Length of query: 331 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 224 Effective length of database: 26,037,693 Effective search space: 5832443232 Effective search space used: 5832443232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)