BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0055.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1395 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permeas... 161 1e-39 lin1484 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 154 1e-37 SA1384 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permeas... 150 2e-36 lin0193 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 144 2e-34 SPy0094 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permea... 133 3e-31 >BH1395 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 287 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 88/225 (39%), Positives = 135/225 (60%), Gaps = 9/225 (4%) Query: 1 LEILFS-DFFQRALAGGLMIGITAPLIGVFLVLRRLSMIGDTLAHVTIAGVALGFLTGFY 59 LE+ F +F Q A G+MIG APL+GVFLV+RR+++I D L+H+T++G+A L Y Sbjct: 2 LEVFFRYEFLQNAFYTGVMIGCLAPLLGVFLVVRRMALIADALSHITLSGIAFSLLISNY 61 Query: 60 --------PLGAGLIFAVVASFAIEKLRKAYKSYAELSIAIIMSGGVALASLFFTLGKGY 111 PL G++F+VV S IEKLR+ YK Y E++I II+SGG+ L+ LF +L G+ Sbjct: 62 AALFLFVNPLYMGIVFSVVGSLLIEKLRQVYKFYQEIAIPIILSGGLGLSVLFLSLADGF 121 Query: 112 NADVMSYLFGSIYTLDNTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAASVSGLPVKL 171 N D+M+YLFGS+ + +D + A VSG+ + Sbjct: 122 NNDLMNYLFGSVIAVSRSDLWTILFITIVVIIVLVLFYKELFFLSFDEEQAVVSGIQRRF 181 Query: 172 LNMLITVLTALVISTAIKIVGSLLVSALLTIPVAISLLLSRSFKS 216 ++ + V+ ALVI+ +++IVG LLVS+L+T+PVA ++ ++ FK Sbjct: 182 VHFIFIVMVALVIAASMRIVGILLVSSLMTLPVAAAMRFAKGFKQ 226 >lin1484 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 292 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 90/245 (36%), Positives = 138/245 (56%), Gaps = 8/245 (3%) Query: 7 DFFQRALAGGLMIGITAPLIGVFLVLRRLSMIGDTLAHVTIAGVALG-FLTGFY------ 59 DF + G++IG+ APL+G F+V+R+LS++ D L+HVT+ G+A+ FL+ Y Sbjct: 9 DFIRNTFIVGIVIGVIAPLLGSFIVVRKLSLMADALSHVTLGGIAVSLFLSKTYLPLAAL 68 Query: 60 -PLGAGLIFAVVASFAIEKLRKAYKSYAELSIAIIMSGGVALASLFFTLGKGYNADVMSY 118 PL G F+VV S +EKLR YK + EL+I IIMS G+ + +F +L G+N D+ SY Sbjct: 69 NPLYLGFGFSVVGSLLMEKLRTVYKHFEELAIPIIMSAGMGFSVIFISLANGFNTDLFSY 128 Query: 119 LFGSIYTLDNTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAASVSGLPVKLLNMLITV 178 LFGS+ + TD + A VSGL K+ +++ V Sbjct: 129 LFGSVSAVSRTDMITIVVTAIIVFIVILSLYKELFLLSFDEEYAKVSGLKAKIFDIIFMV 188 Query: 179 LTALVISTAIKIVGSLLVSALLTIPVAISLLLSRSFKSSVMLSXXXXXXXXXXXXXXXXX 238 L ALVI+++++IVG LLVS+L+T+PVA ++ +++ FK +++LS Sbjct: 189 LVALVIASSMRIVGILLVSSLMTLPVAAAIRIAKGFKQTILLSILFGEIAVIGGLFTAYY 248 Query: 239 WNLAP 243 NLAP Sbjct: 249 LNLAP 253 >SA1384 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 287 Score = 150 bits (379), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 84/223 (37%), Positives = 135/223 (60%), Gaps = 9/223 (4%) Query: 7 DFFQRALAGGLMIGITAPLIGVFLVLRRLSMIGDTLAHVTIAGVALGF--------LTGF 58 DF + +L G++IG APLIG F+V+RRLS+I D L+HVT+ G++ G L Sbjct: 9 DFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADALSHVTLGGISFGMFLLTIMPTLVFI 68 Query: 59 YPLGAGLIFAVVASFAIEKLRKAYKSYAELSIAIIMSGGVALASLFFTLGKGYNADVMSY 118 P+ G++FA+V + IEKLR +Y +Y E++I IIMS G+AL+++F +L G+N +++ Sbjct: 69 NPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPIIMSAGIALSAIFISLADGFNQEIVGL 128 Query: 119 LFGSIYTLDNTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAASVSGLPVKLLNMLITV 178 LFGSI ++ +D + + V G+ K + L V Sbjct: 129 LFGSISAVNISDLTTIIVIAIIVVLFITLFYKELFILSFDEEYSKVIGIH-KWIQFLFIV 187 Query: 179 LTALVISTAIKIVGSLLVSALLTIPVAISLLLSRSFKSSVMLS 221 + A+VIS ++++VG LLVSAL+T+P+AIS+ +++SFK ++LS Sbjct: 188 IVAMVISASMRVVGILLVSALITLPIAISMRITKSFKQLILLS 230 >lin0193 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 268 Score = 144 bits (362), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 83/219 (37%), Positives = 124/219 (56%), Gaps = 1/219 (0%) Query: 1 LEILFSDFFQRALAGGLMIGITAPLIGVFLVLRRLSMIGDTLAHVTIAGVALGFLTGFYP 60 +E+ F QRA ++I I APL+GVFL++RR S++ DTL+HV++AGVA G + P Sbjct: 1 MEMFLYGFMQRAFQASMIIAIIAPLLGVFLIIRRQSLMADTLSHVSLAGVAFGLVLNVNP 60 Query: 61 LGAGLIFAVVASFAIEKLRKAYKSYAELSIAIIMSGGVALASLFFTLGK-GYNADVMSYL 119 LI V+A+ IE LR Y +Y+ELSIAI+M+GG+A+A + +L + G V YL Sbjct: 61 SVTTLIVVVIAALGIEYLRGVYATYSELSIAILMAGGLAVALVLMSLDQGGITTSVQQYL 120 Query: 120 FGSIYTLDNTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAASVSGLPVKLLNMLITVL 179 FGSI T+ D A G+PV+L+++L +V+ Sbjct: 121 FGSIVTISKAQVQLLVILGIAIVVLFLVFKRFMFVLTFSEDVAVAEGVPVRLISLLFSVV 180 Query: 180 TALVISTAIKIVGSLLVSALLTIPVAISLLLSRSFKSSV 218 T + I+ + I G+LLVSAL+ +P AI + +S+ F V Sbjct: 181 TGIAIAVIMPIAGALLVSALIILPAAIGMRISKGFLMCV 219 >SPy0094 [P] COG1108 ABC-type Mn2+/Zn2+ transport systems, permease components Length = 271 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 81/221 (36%), Positives = 128/221 (57%), Gaps = 1/221 (0%) Query: 1 LEILFSDFFQRALAGGLMIGITAPLIGVFLVLRRLSMIGDTLAHVTIAGVALGFLTGFYP 60 L+ILF DF QRA+ + I I AP++G+FL+LRR S++ DTL+HV++AGVALG + G P Sbjct: 2 LDILFYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLSHVSLAGVALGVVLGISP 61 Query: 61 LGAGLIFAVVASFAIEKLRKAYKSYAELSIAIIMSGGVALASLFFTLGKGYNADVM-SYL 119 +I V+A+ +E LR YK Y E+S AI+MS G+AL+ + + ++ + YL Sbjct: 62 TITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALSLIIMSKSHSSSSMSLEQYL 121 Query: 120 FGSIYTLDNTDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDAASVSGLPVKLLNMLITVL 179 FGSI T+ D A V GLPV+L+++L ++ Sbjct: 122 FGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFDEDTAFVDGLPVRLMSVLFNIV 181 Query: 180 TALVISTAIKIVGSLLVSALLTIPVAISLLLSRSFKSSVML 220 T + I+ I G+LLVS ++ +P +I++ L ++FK+ ++L Sbjct: 182 TGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKTVILL 222 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 12,605,588 Number of extensions: 471100 Number of successful extensions: 2158 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 2112 Number of HSP's successfully gapped: 49 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)