BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0053.p (326 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPy0714_1 [P] COG0803 ABC-type metal ion transport system, perip... 209 5e-54 SP2169_1 [P] COG0803 ABC-type metal ion transport system, peripl... 206 3e-53 lin0191 [P] COG0803 ABC-type metal ion transport system, peripla... 197 2e-50 BS_ycdH [P] COG0803 ABC-type metal ion transport system, peripla... 194 2e-49 L167426 [P] COG0803 ABC-type metal ion transport system, peripla... 170 2e-42 >SPy0714_1 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 322 Score = 209 bits (532), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 113/295 (38%), Positives = 161/295 (54%), Gaps = 8/295 (2%) Query: 33 IVEGKINVITTFYPIYEFASEIGGEDIHAINLLPVGVEPHDWTPRSQDIINTSKAQLFLY 92 + EGK+ V+TTFYP+YEF + G D L+ G EPHD+ P ++DI A F+Y Sbjct: 27 LAEGKVKVVTTFYPVYEFTKGVIGNDGDVFMLMKAGTEPHDFEPSTKDIKKIQDADAFVY 86 Query: 93 NGAGLEGWVPNFLKSLDADSEAKAVEVSKGV-DYIMTXXXXXXXXXXXXXXXXXXXXXTD 151 +E WV + KSL +K V + KG + ++ ++ Sbjct: 87 MDDNMETWVSDVKKSL----TSKKVTIVKGTGNMLLVAGAGHDHPHEDADKKHEHNKHSE 142 Query: 152 TLHT---DPHTWVSPRSALIMAENIKESLQSVDPAHKDGYEERYNKLAERLKALDSKFAT 208 H DPH W+SP ++ + ENI++SL P + ++ E+LK LD + Sbjct: 143 EGHNHAFDPHVWLSPYRSITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTA 202 Query: 209 ELAKLPNHEIVVSHQAFAYLARDYGLTQHAIMGLSADAEPRSQDLLKLADLVKKEGIRYI 268 L+ V H AF Y+A DYGL Q +I G++ DAEP ++ + L+ VKK GI+YI Sbjct: 203 ALSDAKQKSFVTQHAAFGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYI 262 Query: 269 FFEELVSDKLAKTLAAEAGVSTMVLNPVEGLTEAQEKNGDNYFTLMEKNLQNLIL 323 +FEE S K+AKTLA EAGV VL+P+EGLTE + K G +YFT+M KNL+ L L Sbjct: 263 YFEENASSKVAKTLAKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRL 317 >SP2169_1 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 310 Score = 206 bits (525), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 116/298 (38%), Positives = 161/298 (54%), Gaps = 12/298 (4%) Query: 24 ACGPKSSGSIVEGKINVITTFYPIYEFASEIGGEDIHAINLLPVGVEPHDWTPRSQDIIN 83 AC S+ +GK+N++TTFYP+YEF ++ G+ + L+ G EPH++ P ++ + Sbjct: 18 AC---SNQKQADGKLNIVTTFYPVYEFTKQVAGDTANVELLIGAGTEPHEYEPSAKAVAK 74 Query: 84 TSKAQLFLYNGAGLEGWVPNFLKSLDADSEAKAVEVSKGVDYIMTXXXXXXXXXXXXXXX 143 A F+Y +E WVP L +LD K V+ K T Sbjct: 75 IQDADTFVYENENMETWVPKLLDTLDK----KKVKTIKA-----TGDMLLLPGGEEEEGD 125 Query: 144 XXXXXXTDTLHTDPHTWVSPRSALIMAENIKESLQSVDPAHKDGYEERYNKLAERLKALD 203 DPH W+SP A+ + E+I++SL + P K+ +E+ E+L+ALD Sbjct: 126 HDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLSADYPDKKETFEKNAAAYIEKLQALD 185 Query: 204 SKFATELAKLPNHEIVVSHQAFAYLARDYGLTQHAIMGLSADAEPRSQDLLKLADLVKKE 263 +A L++ V H AF YLA DYGL Q AI GLS DAEP + L +L + VKK Sbjct: 186 KAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQVAISGLSPDAEPSAARLAELTEYVKKN 245 Query: 264 GIRYIFFEELVSDKLAKTLAAEAGVSTMVLNPVEGLTEAQEKNGDNYFTLMEKNLQNL 321 I YI+FEE S LA TL+ EAGV T VLNP+E LTE K G+NY ++MEKNL+ L Sbjct: 246 KIAYIYFEENASQALANTLSKEAGVKTDVLNPLESLTEEDTKAGENYISVMEKNLKAL 303 >lin0191 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 312 Score = 197 bits (501), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 112/302 (37%), Positives = 159/302 (52%), Gaps = 16/302 (5%) Query: 23 AACGP---KSSGSIVEGKINVITTFYPIYEFASEIGGEDIHAINLLPVGVEPHDWTPRSQ 79 A CG K+SG K+ V+TTFYP+Y+F + G++ L+ G EPHD+ P ++ Sbjct: 19 AGCGASADKASGD----KLKVVTTFYPMYDFTKNVAGDNASIDMLIDAGTEPHDYEPSAK 74 Query: 80 DIINTSKAQLFLYNGAGLEGWVPNFLKSLDADSEAKAVEVSKGVDYIMTXXXXXXXXXXX 139 DI A +F+YN +E WVP+ LKSLD+ + ++ SKG+ + Sbjct: 75 DIAKIEAADVFIYNSEDMETWVPSVLKSLDS-KKLTIIDASKGIKLL--------EGSEE 125 Query: 140 XXXXXXXXXXTDTLHTDPHTWVSPRSALIMAENIKESLQSVDPAHKDGYEERYNKLAERL 199 DPH W+SP A +NI+ L D A+ D Y++ K ++L Sbjct: 126 EEHDHDHGEEGHHHEHDPHVWLSPVLAEKEVKNIQSGLSKADKANADTYKKNAEKYTDKL 185 Query: 200 KALDSKFATELAKLPNHEIVVSHQAFAYLARDYGLTQHAIMGLSADAEPRSQDLLKLADL 259 K LD+KF T + V H AF YLA +Y L Q AI GLS D EP L +L Sbjct: 186 KTLDNKFKTAFEGAKQRDFVTQHAAFQYLANEYDLHQVAIAGLSPDQEPSPARLAELQKY 245 Query: 260 VKKEGIRYIFFEELVSDKLAKTLAAEAGVSTMVLNPVEGLTEAQEKNGDNYFTLMEKNLQ 319 VK+ I I+FEE+ S K+A+ LA E G VL+P+EG+T+ ++K G +Y ME+NLQ Sbjct: 246 VKENNISTIYFEEVASPKVAEALANETGAKLEVLSPIEGITDKEQKKGMDYIAYMEQNLQ 305 Query: 320 NL 321 L Sbjct: 306 AL 307 >BS_ycdH [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 152 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 0/302 (0%), Positives = 0/302 (0%), Gaps = 12/302 (3%) Query: 23 AACGPKSSGSIVEGK---INVITTFYPIYEFASEIGGEDIHAINLLPVGVEPHDWTPRSQ 79 A S+ V + V A + + ++ Sbjct: 19 RAGFTASAVCSVTDTPPTLLVCLNRGASVWPAFNENRTLCVNTLSAGQEPLSNLFGGKTP 78 Query: 80 DIINTSKAQLFLYNGAGLEGWVPNFLKSLDADSEAKAVEVSKGVDYIMTXXXXXXXXXXX 139 + A+ + S+ +V + + Sbjct: 79 MEHRFAAARWQTGVTGCPQLEEALVSFDC-RISQVVSVGTHDILFCAIEAIHRHTTPYGL 137 Query: 140 XXXXXXXXXXTDTLHTDPHTWVSPRSALIMAENIKESLQSVDPAHKDGYEERYNKLAERL 199 Sbjct: 138 VWFDRSY-------- 189 Query: 200 KALDSKFATELAKLPNHEIVVSHQAFAYLARDYGLTQHAIMGLSADAEPRSQDLLKLADL 259 Sbjct: 190 249 Query: 260 VKKEGIRYIFFEELVSDKLAKTLAAEAGVSTMVLNPVEGLTEAQEKNGDNYFTLMEKNLQ 319 Sbjct: 250 309 Query: 320 NL 321 Sbjct: 310 311 >L167426 [P] COG0803 ABC-type metal ion transport system, periplasmic component/surface adhesin Length = 280 Score = 170 bits (431), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 101/299 (33%), Positives = 148/299 (49%), Gaps = 40/299 (13%) Query: 23 AACGPKSSGSIVEGKINVITTFYPIYEFASEIGGEDIHAINLLPVGVEPHDWTPRSQDII 82 A C +S K V+TTF P+YEF I G+ + N++P E H++ P ++ + Sbjct: 17 AGCQKSAS------KPEVVTTFEPMYEFTKAIVGDKVDVENIVPANQEVHEFEPSAKQVA 70 Query: 83 NTSKAQLFLYNGAGLEGWVPNFLKSLDADSEAKAVEVSKGVDYIMTXXXXXXXXXXXXXX 142 + A+ +YN LE W +++ +E SK VD I Sbjct: 71 TMTNAEAIVYNSNDLEKWAKKV------NNKGIKIEASKDVDKI---------------- 108 Query: 143 XXXXXXXTDTLHTDPHTWVSPRSALIMAENIKESLQSVDPAHKDGYEERYNKLAERLKAL 202 DPHTW+SP+ A+I I L P K +E+ +LK L Sbjct: 109 -----------SGDPHTWISPKQAIIEVNTIASELGKKFPDDKATFEKNAASYVAKLKKL 157 Query: 203 DSKFATELAKLPNHEIVVSHQAFAYLARDYGLTQHAIMGLSADAEPRSQDLLKLADLVKK 262 DS+F T L + H+AFAYL+RDYGL + AI GL + EP + L L +KK Sbjct: 158 DSEFDT-LKDAKQKTFITQHEAFAYLSRDYGLKEIAIAGLDPEVEPSASTLANLKVEMKK 216 Query: 263 EGIRYIFFEELVSDKLAKTLAAEAGVSTMVLNPVEGLTEAQEKNGDNYFTLMEKNLQNL 321 G++ ++FE + K+A+TLA G + + +N VEGL++ Q+KNGDNY TLM+ NL L Sbjct: 217 AGLKNVYFEGNANSKIAETLAKSVGANLIGINTVEGLSDEQKKNGDNYLTLMQDNLTAL 275 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,644,936 Number of extensions: 767338 Number of successful extensions: 2701 Number of sequences better than 1.0e-05: 39 Number of HSP's gapped: 2660 Number of HSP's successfully gapped: 52 Length of query: 326 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 219 Effective length of database: 26,037,693 Effective search space: 5702254767 Effective search space used: 5702254767 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 116 (49.3 bits)