BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0049.p (158 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_zur [P] COG0735 Fe2+/Zn2+ uptake regulation proteins 124 7e-29 BH1396 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins 115 2e-26 SA1383 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins 106 2e-23 lin1483 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins 103 8e-23 SA1678 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins 77 8e-15 >BS_zur [P] COG0735 Fe2+/Zn2+ uptake regulation proteins Length = 191 Score = 124 bits (310), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 4/144 (2%), Positives = 24/144 (16%), Gaps = 1/144 (0%) Query: 7 LSTDQILEAMSGQGLRITDQRKTLAKLFGENTGYLSAKDVYEHMGRKYSGLSFDTVYRNL 66 + + + + + + + + + ++ Sbjct: 1 MFFLEHGKVRTFLTPTLRCPMEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDG 60 Query: 67 RVMEELGVLEQVVFEDGVKFKGSCNQDHHHHHMICLQCMKTYPIHFCPMN-LTDTPDQFR 125 + + E K + +++ + T Sbjct: 61 AISAYDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAM 120 Query: 126 VVKHKFEVFGYCKECEQHREEETA 149 + + T Sbjct: 121 FICDRCGAVKEECAEGVEDIMHTL 144 >BH1396 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins Length = 138 Score = 115 bits (289), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 57/135 (42%), Positives = 89/135 (65%), Gaps = 1/135 (0%) Query: 7 LSTDQILEAMSGQGLRITDQRKTLAKLFGENTGYLSAKDVYEHMGRKYSGLSFDTVYRNL 66 ++ + + + +G + TD+R+ + +F + + YLSAKDV E + ++Y GLSFDT+YRNL Sbjct: 1 MNVSEAMNILKNKGYKHTDKRELMLTIFADESRYLSAKDVLERLQKQYPGLSFDTIYRNL 60 Query: 67 RVMEELGVLEQVVFEDGVKFKGSCNQDHHHHHMICLQCMKTYPIHFCPMNLTDTP-DQFR 125 + +LG+LE+ + +F+ SC+ HHHHH+ICL C KT I CPM++ + +F Sbjct: 61 SLFSQLGILEETELDGEKRFRFSCSTTHHHHHLICLTCGKTKHIENCPMDIVNQSFPEFE 120 Query: 126 VVKHKFEVFGYCKEC 140 V HKFE++GYCK C Sbjct: 121 VTGHKFEIYGYCKSC 135 >SA1383 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins Length = 136 Score = 106 bits (264), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 49/136 (36%), Positives = 85/136 (62%), Gaps = 2/136 (1%) Query: 7 LSTDQILEAMSGQGLRITDQRKTLAKLFGENTGYLSAKDVYEHMGRKYSGLSFDTVYRNL 66 ++T+ ++ + GL+ TD+RK + +F E Y++AK + + M Y G+SFDT+YRNL Sbjct: 1 MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVMDENYPGISFDTIYRNL 60 Query: 67 RVMEELGVLEQVVFEDGVKFKGSCNQDHHHHHMICLQCMKTYPIHFCPMN-LTDTPDQFR 125 + ++LG++E + +KF+ +C +HHHHH IC +C T I +CP++ + + Sbjct: 61 HLFKDLGIIENTELDGEMKFRIACT-NHHHHHFICEKCGDTKVIDYCPIDQIKLSLPGVN 119 Query: 126 VVKHKFEVFGYCKECE 141 + KHK EV+G C+ C+ Sbjct: 120 IHKHKLEVYGVCESCQ 135 >lin1483 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins Length = 141 Score = 103 bits (258), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 58/137 (42%), Positives = 83/137 (60%), Gaps = 1/137 (0%) Query: 7 LSTDQILEAMSGQGLRITDQRKTLAKLFGENTGYLSAKDVYEHMGRKYSGLSFDTVYRNL 66 L+ + L M +G + TD+R+ L L YL+AKDV E+M + G+SFDT+YRNL Sbjct: 3 LTATEALMKMKEKGYKHTDKREFLINLLARKNKYLTAKDVLENMKDDFPGISFDTIYRNL 62 Query: 67 RVMEELGVLEQVVFEDGVKFKGSCNQDHHHHHMICLQCMKTYPIHFCPMN-LTDTPDQFR 125 + ELG+ E+ F+ +C +HHHHH IC++C KT I CPM+ LT+ ++ Sbjct: 63 SLFVELGIFEETDLSGERNFRLACTHEHHHHHFICMKCGKTKEIMMCPMDFLTEALPGYQ 122 Query: 126 VVKHKFEVFGYCKECEQ 142 + HKFEV+G C EC Q Sbjct: 123 IDGHKFEVYGECPECLQ 139 >SA1678 [P] COG0735 Fe2+/Zn2+ uptake regulation proteins Length = 148 Score = 77.4 bits (189), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 37/134 (27%), Positives = 75/134 (55%), Gaps = 8/134 (5%) Query: 13 LEAMSGQGLRITDQRKTLAKLFGENTGYLSAKDVYEHMGRKYSGLSFDTVYRNLRVMEEL 72 + ++ G+RIT QR+ + + + + +A ++Y+ + + +S T+Y NLRV +++ Sbjct: 16 IASLRQAGIRITPQRQAILRYLISSHTHPTADEIYQALSPDFPNISVATIYNNLRVFKDI 75 Query: 73 GVLEQVVFED-GVKFKGSCNQDHHHHHMICLQCMKTYPIHFCPMNLTDTPDQ----FRVV 127 G+++++ + D +F + H+H+H+IC QC K + +N + Q F V Sbjct: 76 GIVKELTYGDSSSRFDFNT---HNHYHIICEQCGKIVDFQYPQLNEIERLAQHMTDFDVT 132 Query: 128 KHKFEVFGYCKECE 141 H+ E++G CKEC+ Sbjct: 133 HHRMEIYGVCKECQ 146 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,460,407 Number of extensions: 433778 Number of successful extensions: 1516 Number of sequences better than 1.0e-05: 372 Number of HSP's gapped: 1144 Number of HSP's successfully gapped: 372 Length of query: 158 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 60 Effective length of database: 27,075,699 Effective search space: 1624541940 Effective search space used: 1624541940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 111 (47.4 bits)