BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0047.p (483 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1339 [GEPR] COG0477 Permeases of the major facilitator superf... 371 e-102 BS_araE [GEPR] COG0477 Permeases of the major facilitator superf... 370 e-102 sll0771 [GEPR] COG0477 Permeases of the major facilitator superf... 293 3e-79 CC0814 [GEPR] COG0477 Permeases of the major facilitator superfa... 263 7e-70 BS_araE [GEPR] COG0477 Permeases of the major facilitator superf... 262 9e-70 >CAC1339 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 469 Score = 371 bits (952), Expect = e-102, Method: Compositional matrix adjust. Identities = 190/459 (41%), Positives = 285/459 (62%), Gaps = 5/459 (1%) Query: 23 KNEKPNMLFVTLVSIVAALGGILFGFDIAVVSGAVGLLQQHFALSEFQVGWAVSSLIVGS 82 + K ++LF+ L+S A LGG+L+G+D AV+SGA+G L++ + LS G+ +SS++VG Sbjct: 5 NSHKHSLLFIVLISCAAGLGGLLYGYDTAVISGAIGFLKKLYNLSPAMQGFVISSIMVGG 64 Query: 83 VTGAALSGYMSERFGRKKVLLAAAILFIVGSVGSALQDTFTGYIIARMXXXXXXXXTSTI 142 V G SG++ + GR+KVL+ AA LF + +V S++ + I AR+ S + Sbjct: 65 VLGVGFSGFLGDAIGRRKVLMLAAALFAISAVISSISTSAFMLIFARIVGGLGIGMASAL 124 Query: 143 CPVYNAEIAPAKYRGRLVALNQLAIVSGIFLVYFLNLWIVGMGDEAWDASTAWRWMFGVG 202 Y E AP RGRL +L QL + GI + +F+NL IV MG E W ST WR+M G Sbjct: 125 SVTYITECAPPSIRGRLSSLYQLFTILGISITFFVNLGIVNMGSETWRVSTGWRYMLACG 184 Query: 203 AVPGLLFMILMLFIPESPRWLIKRNRPYEALPILVKIHGEEAAXXXXKQEVLDIKESFNN 262 VP ++F+I + F+PESPR+L+K +A +L KI+G E A KQE+ I +S Sbjct: 185 TVPAIVFLITLFFVPESPRFLVKSGNIKKAAAVLTKINGAEIA----KQELDSISKSLAT 240 Query: 263 ENDS-FKQLFTPGIRSALFIGVMLAVMQHITGINAILYYAPEIFKGMGLGTDASLTQTIW 321 ENDS QL PG+R AL IG+ LA+ G+N+I YY PEIF+ +G ++S T Sbjct: 241 ENDSSLGQLLQPGLRRALLIGIFLAIFNQAIGMNSITYYGPEIFQMIGFKNNSSFLATSV 300 Query: 322 IGLINVLFTIISVWLIDKVGRKALLLTGTSLMTLCLAIIGAAFQMDLTTGPLVLIVILIY 381 IG++ V TI++++LIDK+GRK L+ G++ M + + +IG +F + L+ G ++LI I+ + Sbjct: 301 IGVVEVFSTILAMFLIDKLGRKKLMEIGSAAMAVFMLLIGTSFYIKLSNGFVILIFIICF 360 Query: 382 VASFAISLGPVVWVMLSEIFPNRIRGKAVAIASMALWAGDYLVSQLFPPLLSSAGPANTF 441 V SF IS+GP+ W+M+ EIFPN +R +A IA++ LW ++ + Q P LL+ G A TF Sbjct: 361 VVSFCISMGPIPWIMIPEIFPNHLRARATGIATIFLWGANWAIGQFTPMLLNGIGGAYTF 420 Query: 442 WIFGIASLFSLLFIWRAVPETKGRSLEQMEAMWTGKGSE 480 WIF ++ L + VPETK +SLE++E W K + Sbjct: 421 WIFCGINVICFLVVTTKVPETKNKSLEEIEKFWIPKSKQ 459 >BS_araE [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 464 Score = 370 bits (949), Expect = e-102, Method: Compositional matrix adjust. Identities = 194/448 (43%), Positives = 291/448 (64%), Gaps = 5/448 (1%) Query: 28 NMLFVTLVSIVAALGGILFGFDIAVVSGAVGLLQQHFALSEFQVGWAVSSLIVGSVTGAA 87 +M FV L+S A LGG+L+G+D AV+SGA+G L+ ++LS F G +SS+++G V G Sbjct: 19 SMGFVILISCAAGLGGLLYGYDTAVISGAIGFLKDLYSLSPFMEGLVISSIMIGGVVGVG 78 Query: 88 LSGYMSERFGRKKVLLAAAILFIVGSVGSALQDTFTGYIIARMXXXXXXXXTSTICPVYN 147 +SG++S+RFGR+K+L+ AA+LF + ++ SAL + IIAR+ S++ Y Sbjct: 79 ISGFLSDRFGRRKILMTAALLFAISAIVSALSQDVSTLIIARIIGGLGIGMGSSLSVTYI 138 Query: 148 AEIAPAKYRGRLVALNQLAIVSGIFLVYFLNLWIVGMGDEAWDASTAWRWMFGVGAVPGL 207 E AP RG L +L QL + GI YF+NL + G W T WRWM G VP + Sbjct: 139 TEAAPPAIRGSLSSLYQLFTILGISATYFINLAVQRSGTYEWGVHTGWRWMLAYGMVPSV 198 Query: 208 LFMILMLFIPESPRWLIKRNRPYEALPILVKIHGEEAAXXXXKQEVLDIKESFNNEN-DS 266 +F +++L +PESPRWL K + EAL IL +I+GE A K+E+ +I+ S E S Sbjct: 199 IFFLVLLVVPESPRWLAKAGKTNEALKILTRINGETVA----KEELKNIENSLKIEQMGS 254 Query: 267 FKQLFTPGIRSALFIGVMLAVMQHITGINAILYYAPEIFKGMGLGTDASLTQTIWIGLIN 326 QLF PG+R AL IG++LA+ + G+NAI YY PEIFK MG G +A T +G++ Sbjct: 255 LSQLFKPGLRKALVIGILLALFNQVIGMNAITYYGPEIFKMMGFGQNAGFVTTCIVGVVE 314 Query: 327 VLFTIISVWLIDKVGRKALLLTGTSLMTLCLAIIGAAFQMDLTTGPLVLIVILIYVASFA 386 V+FT+I+V LIDKVGRK L+ G++ M + + +IG +F +LT+G +++++IL +VA+F Sbjct: 315 VIFTVIAVLLIDKVGRKKLMSIGSAFMAIFMILIGTSFYFELTSGIMMIVLILGFVAAFC 374 Query: 387 ISLGPVVWVMLSEIFPNRIRGKAVAIASMALWAGDYLVSQLFPPLLSSAGPANTFWIFGI 446 +S+GP+ W+M+SEIFPN +R +A IA++ LW ++ + Q P ++ S G A TFWIF + Sbjct: 375 VSVGPITWIMISEIFPNHLRARAAGIATIFLWGANWAIGQFVPMMIDSFGLAYTFWIFAV 434 Query: 447 ASLFSLLFIWRAVPETKGRSLEQMEAMW 474 ++ LF+ PETK +SLE++E +W Sbjct: 435 INILCFLFVVTICPETKNKSLEEIEKLW 462 >sll0771 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 468 Score = 293 bits (751), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 179/477 (37%), Positives = 271/477 (56%), Gaps = 27/477 (5%) Query: 12 INKEISQTKALKNEKPNMLFVTLVSIVAALGGILFGFDIAVVSGAVGLLQQHFALSEFQV 71 +N S +++ N K FV L+S VAALGG LFGFD AV++GAV LQ+HF Sbjct: 1 MNPSSSPSQSTANVK----FVLLISGVAALGGFLFGFDTAVINGAVAALQKHFQTDSLLT 56 Query: 72 GWAVSSLIVGSVTGAALSGYMSERFGRKKVLLAAAILFIVGSVGSALQDTFTGYIIARMX 131 G +VS ++GS GA +G +++R GR K ++ AA+LF + S+GS L T +I R+ Sbjct: 57 GLSVSLALLGSALGAFGAGPIADRHGRIKTMILAAVLFTLSSIGSGLPFTIWDFIFWRVL 116 Query: 132 XXXXXXXTSTICPVYNAEIAPAKYRGRLVALNQLAIVSGIFLV----YFLNLWIVGMGDE 187 S I P Y AE++PA RGRL +L QLAIVSGIF+ +F+ L G Sbjct: 117 GGIGVGAASVIAPAYIAEVSPAHLRGRLGSLQQLAIVSGIFIALLSNWFIALMAGGSAQN 176 Query: 188 AW-DASTAWRWMFGVGAVPGLLFMILMLFIPESPRWLIKRNRPYEALPILVKIHGEEAAX 246 W + AWRWMF +P LL+ + IPESPR+L+ + + +A IL K+ G + Sbjct: 177 PWLFGAAAWRWMFWTELIPALLYGVCAFLIPESPRYLVAQGQGEKAAAILWKVEGGDV-- 234 Query: 247 XXXKQEVLDIKESFN-NENDSFKQLFTP--GIRSALFIGVMLAVMQHITGINAILYYAPE 303 + +I+ + + + F L + G+ ++IG+ L+ +Q GIN I YY+ Sbjct: 235 ---PSRIEEIQATVSLDHKPRFSDLLSRRGGLLPIVWIGMGLSALQQFVGINVIFYYSSV 291 Query: 304 IFKGMGLGTDASLTQTIWIGLINVLFTIISVWLIDKVGRKALLLTGTSLMTLCLAIIGAA 363 +++ +G + SL T+ G IN+L T++++ +DK GRK LLL G+ MT+ L I+ Sbjct: 292 LWRSVGFTEEKSLLITVITGFINILTTLVAIAFVDKFGRKPLLLMGSIGMTITLGILSVV 351 Query: 364 F--------QMDLT--TGPLVLIVILIYVASFAISLGPVVWVMLSEIFPNRIRGKAVAIA 413 F Q LT G + L+ +YV SF S GP+VWV+L E+F N+IR A+++A Sbjct: 352 FGGATVVNGQPTLTGAAGIIALVTANLYVFSFGFSWGPIVWVLLGEMFNNKIRAAALSVA 411 Query: 414 SMALWAGDYLVSQLFPPLLSSAGPANTFWIFGIASLFSLLFIWRAVPETKGRSLEQM 470 + W ++++S FPPLL + G + ++ ++ S+ FIW V ETKG++LEQM Sbjct: 412 AGVQWIANFIISTTFPPLLDTVGLGPAYGLYATSAAISIFFIWFFVKETKGKTLEQM 468 >CC0814 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 519 Score = 263 bits (671), Expect = 7e-70, Method: Compositional matrix adjust. Identities = 157/487 (32%), Positives = 269/487 (55%), Gaps = 24/487 (4%) Query: 3 GRLTEMAIVINKEISQTKALKNEKPNMLFVTLVSIVAALGGILFGFDIAVVSGAVGLLQQ 62 GR T +A V N S + K NM FV + VA +GG +FG+D V++G L+ Sbjct: 38 GR-TPVASVSNAGPSAGMSADGGKVNMTFVAAIVAVATIGGFMFGYDSGVINGTQEGLES 96 Query: 63 HFALSEFQVGWAVSSLIVGSVTGAALSGYMSERFGRKKVLLAAAILFIVGSVGSALQDTF 122 F LS+ G V ++++G GA +G +++ +GR+ V++ +A+LF++ ++G+ ++ Sbjct: 97 AFNLSKLGTGLNVGAILIGCAIGAFAAGRLADVWGRRTVMIISALLFVISAIGTGAAESS 156 Query: 123 TGYIIARMXXXXXXXXTSTICPVYNAEIAPAKYRGRLVALNQLAIVSGIFLVYFLNLWIV 182 +II R+ S +CPVY +E+ PA RGRL ++ Q+ I++G+ + N + Sbjct: 157 IVFIIFRLIGGLGVGAASVLCPVYISEVTPANIRGRLSSVQQIMIITGLTGAFVANYALA 216 Query: 183 GMGD----EAWDASTAWRWMFGVGAVPGLLFMILMLFIPESPRWLIKRNRPYEALPILVK 238 E W AWRWMF + +P +F + +L IPESPR+L+ + + +A IL + Sbjct: 217 HTAGSSTAEFWLGLPAWRWMFWMQIIPAGVFFLCLLGIPESPRYLVAKGKDAQAEAILSR 276 Query: 239 IHGEEAAXXXXKQEVLDIKESFNNEND-SFKQLFTP---GIRSALFIGVMLAVMQHITGI 294 + G ++V +I+ S + ++ +F L P +R L+ G++LAV Q + GI Sbjct: 277 LFGAGQG----AKKVEEIRASLSADHKPTFSDLLDPTTKKLRVILWAGLVLAVFQQLVGI 332 Query: 295 NAILYYAPEIFKGMGLGTDASLTQTIWIGLINVLFTIISVWLIDKVGRKALLLTGTSLMT 354 N + YY +++ +G D SL I G +++L ++++ LIDK+GRK LLL G++ M Sbjct: 333 NIVFYYGSVLWQSVGFTEDDSLKINILSGTLSILACLLAIGLIDKIGRKPLLLIGSAGMA 392 Query: 355 LCLAIIGAAFQMDLTT----------GPLVLIVILIYVASFAISLGPVVWVMLSEIFPNR 404 + L ++ F T G LI +YV F +S GPV+WVML E+FPN+ Sbjct: 393 VTLGVLTWCFSTATTVNGALTLGDQIGLTALIAANLYVIFFNLSWGPVMWVMLGEMFPNQ 452 Query: 405 IRGKAVAIASMALWAGDYLVSQLFPPLLSSAGPANTFWIFGIASLFSLLFIWRAVPETKG 464 +RG A+A+ A W ++ +S FP L +++ P T+ + ++++ S + + V ET+G Sbjct: 453 MRGSALAVCGFAQWIANFAISVSFPALAAASLPM-TYGFYALSAVVSFFLVQKLVHETRG 511 Query: 465 RSLEQME 471 + LE M+ Sbjct: 512 KELEAMQ 518 Score = 262 bits (670), Expect = 9e-70, Method: Compositional matrix adjust. Identities = 32/475 (6%), Positives = 93/475 (19%), Gaps = 31/475 (6%) Query: 10 IVINKEISQT-KALKNEKPNMLFVTLVSIVAALGGILFGFDIAVVSGAVGLLQQHFALSE 68 ++ ++ + + AA G L V + Sbjct: 2 KNTPTQLEPNVPVTRSHSMGFVIL---ISCAAGLGGLLYGYDTAVISGAIGFLKDLYSLS 58 Query: 69 FQVGWAVSSLIVGSVTGAALSGYMSERFGRKKVLLAAAILFIVGSVGSALQDTFTGYIIA 128 + V S I+ ++ +L A L S + +I Sbjct: 59 PFMEGLVISSIMIGGVVGVGISGFLSDRFGRRKILMTAALLFAISAIVSALSQDVSTLII 118 Query: 129 RMXXXXXXXXTSTICPVYNAEIAPAKYRGRLVALNQLAIVSGIFLVYFLNLWIVGMGDEA 188 + V A ++ Sbjct: 119 ARIIGGLGIGMGSSLSVTYITEAAPPAIRGSLSSLYQLFTILGISAT--------YFINL 170 Query: 189 WDASTAWRWMFGVGAVPGLLFMILMLFIPESPRWLIKRNRPYEALPIL--VKIHGEEAAX 246 + +L ++ + L+ P + Sbjct: 171 AVQRSGTYEWGVHTGWRWMLAYGMVPSVIFFLVLLVVPESPRWLAKAGKTNEALKILT-- 228 Query: 247 XXXKQEVLDIKESFNNENDS---FKQLFTPGIRSALFIGVMLAVMQHITGINAILYYAPE 303 + + + ++ + + G+ A++ I Sbjct: 229 ---RINGETVAKEELKNIENSLKIE--QMGSLSQLFKPGLRKALVIGILLALFNQVIGMN 283 Query: 304 IFKGMGL-GTDASLTQTIWIGLINVLFTIISVWLIDKVGRKALLLTGTSLMTLCLAIIGA 362 G + + ++ V + LM++ A + Sbjct: 284 AITYYGPEIFKMMGFGQNAGFVTTCIVGVVEVIFTVIAVLLIDKVGRKKLMSIGSAFMAI 343 Query: 363 AF-QMDLTTGP-----LVLIVILIYVASFAISLGPVVWVMLSEIFPNRIRGKAVAIASMA 416 + + +++IV+++ + + ++ A + Sbjct: 344 FMILIGTSFYFELTSGIMMIVLILGFVAAFCVSVGPITWIMISEIFPNHLRARAAGIATI 403 Query: 417 LWAGDYLVSQLFPPLLSSAGPANTFWIFGIASLFSLLFIWRAVPETKGRSLEQME 471 G F P++ + + + + Sbjct: 404 FLWGANWAIGQFVPMMIDSFGLAYTFWIFAVINILCFLFVVTICPETKNKSLEEI 458 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,398,624 Number of extensions: 1297901 Number of successful extensions: 4801 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 4765 Number of HSP's successfully gapped: 41 Length of query: 483 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 373 Effective length of database: 25,691,691 Effective search space: 9583000743 Effective search space used: 9583000743 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)