BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0041.p (276 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yurM [G] COG0395 ABC-type sugar transport system, permease co... 170 2e-42 BH1119 [G] COG0395 ABC-type sugar transport system, permease com... 130 2e-30 lin2970 [G] COG0395 ABC-type sugar transport system, permease co... 129 5e-30 BH1246 [G] COG0395 ABC-type sugar transport system, permease com... 129 6e-30 all5282 [G] COG0395 ABC-type sugar transport system, permease co... 124 1e-28 >BS_yurM [G] COG0395 ABC-type sugar transport system, permease component Length = 300 Score = 170 bits (431), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 86/246 (34%), Positives = 146/246 (59%), Gaps = 4/246 (1%) Query: 32 SVKKDNNAIALNPFGIPKEFVFNNYVEAWVNAKISTYFFNXXXXXXXXXXXXXXXXXMFA 91 S K+++ I + + +P + N+V AW N IS+YF N A Sbjct: 58 SAFKNSDDIFEHSWSLPSSWHPENFVSAW-NQGISSYFMNSVIVTALTCVITVFISAWAA 116 Query: 92 FAVTRMR-QGKWNKILFSLVLIGMLIPNNALMLPIYTIVRKLHILNTHWALIIPYIANAI 150 + ++R +GK L L G+++ ++P+Y+I++ L + NT+WALI+PY A I Sbjct: 117 YGLSRFEFKGK--GFFLVLCLGGLMLTPQVSLVPLYSIIQSLGLYNTYWALILPYAAYRI 174 Query: 151 PFTIIILAAFMRSLPSEIEEAAVVDGLKAPGIFARIIVPLTVPAMVTVFIVNFLGNWNEF 210 PFTII++ ++ S+ E+EEAA +DG + G+F RI +P++VP +VT I+ WNEF Sbjct: 175 PFTIILIRSYFLSISKELEEAAYLDGCTSFGVFFRIFLPMSVPILVTSGILTAYHTWNEF 234 Query: 211 LLANYFLSNDELRTLPVGMVQFRDQYQMNYAQMSAGIVYSVLPVVIIYAILQEKIIEGVT 270 + A F+ ++ LRT+P G++QFRD Q ++ + AG+ S P++I++ ++Q+ + G+ Sbjct: 235 MFAIIFIDDENLRTIPAGLMQFRDALQTDWGVLLAGLTISAAPIIILFLLMQKYFVRGIA 294 Query: 271 AGSVKG 276 +GSVKG Sbjct: 295 SGSVKG 300 >BH1119 [G] COG0395 ABC-type sugar transport system, permease component Length = 281 Score = 130 bits (327), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 74/249 (29%), Positives = 130/249 (52%), Gaps = 5/249 (2%) Query: 31 SSVKKDNNAIALNPFGIPKEFVFNNYVEAWVN---AKISTYFFNXXXXXXXXXXXXXXXX 87 SS+K + + IP EFV++NY + W YF N Sbjct: 34 SSLKPASEIFSQASSLIPSEFVWSNYPDGWAGFGRTGFDIYFKNSFIITTSVVIGAILSS 93 Query: 88 XMFAFAVTRMRQGKWNKILFSLVLIGMLIPNNALMLPIYTIVRKLHILNTHWALIIPYIA 147 + A+ R+ Q K+ K+LF+ ++ +++P ++P Y + L +NT + LI+P Sbjct: 94 SIVAYGFARL-QFKFKKVLFACLIATVMLPVQITLIPQYILFHNLGWVNTFYPLIVPAFL 152 Query: 148 NAIPFTIIILAAFMRSLPSEIEEAAVVDGLKAPGIFARIIVPLTVPAMVTVFIVNFLGNW 207 PF I +L F+R +P E++EAA++DG G+F R+I+PL PA+VTV I F+ W Sbjct: 153 GGTPFFIFLLIQFIRGIPRELDEAAIIDGCSTFGVFWRVILPLLKPALVTVAIFAFMWTW 212 Query: 208 NEFLLANYFLSNDELRTLPVGMVQFRD-QYQMNYAQMSAGIVYSVLPVVIIYAILQEKII 266 ++FL +L+ +++T+ +G+ F D + ++ + A S+LP II+ Q ++ Sbjct: 213 DDFLAPLIYLNKTDIQTVALGLRNFMDAESGTSWGALLAMSTLSLLPQFIIFLFFQRYLV 272 Query: 267 EGVTAGSVK 275 EG++ +K Sbjct: 273 EGISTTGLK 281 >lin2970 [G] COG0395 ABC-type sugar transport system, permease component Length = 283 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 82/253 (32%), Positives = 142/253 (56%), Gaps = 13/253 (5%) Query: 31 SSVKKDNNAIALNPFGIPKEFVFNNYVEAWVNAKIS---TYFFNXXXXXXXXXXXXXXXX 87 +S K AI+ +P PK+F F +Y++ + N I TYF N Sbjct: 37 TSFKSSKEAISTHPTFFPKDFTFQHYIDIF-NPDIFPFLTYFKNSLVVSVFAAGIAVVLG 95 Query: 88 XMFAFAVTRMR-QGKW--NKILFSLVLIGMLIPNNALMLPIYTIVRKLHILNTHWALIIP 144 + A+A++++R +G+ N +++ + ++ L++P++ I+ L + +T ALII Sbjct: 96 ILGAYALSKLRFKGRMTINASFYTVYMFSGIL----LIVPLFKIISSLGLYDTETALIIT 151 Query: 145 YIANAIPFTIIILAAFMRSLPSEIEEAAVVDGLKAPGIFARIIVPLTVPAMVTVFIVNFL 204 + +P + +L ++ ++P++IEEAA++DGL I RIIVPL + +V+VF+ F+ Sbjct: 152 MVVQTLPTAVFMLKSYFDTIPTDIEEAAMMDGLNRFQIILRIIVPLAISGIVSVFVYCFM 211 Query: 205 GNWNEFLLANYFLSNDELRTLPVGMVQFRDQYQMNYAQMSAGIVYSVLPVVIIYAILQEK 264 WN++L A+ FLS+ E TLP+G+ + +M A + + LPVVI+YAI E+ Sbjct: 212 VAWNDYLFASIFLSSSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVVIMYAI-SER 270 Query: 265 IIEG-VTAGSVKG 276 I+G +T G VKG Sbjct: 271 FIKGNLTDGGVKG 283 >BH1246 [G] COG0395 ABC-type sugar transport system, permease component Length = 273 Score = 129 bits (323), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 79/227 (34%), Positives = 125/227 (55%), Gaps = 3/227 (1%) Query: 50 EFVFNNYVEAWVNAKISTYFFNXXXXXXXXXXXXXXXXXMFAFAVTRMR-QGKWNKILFS 108 E F +YV A N + TY N + A+A+TR+ +GK ++ Sbjct: 48 EPTFQSYVNAVNNRPLFTYMMNSFVISSLTTLIAIACASLAAYALTRLPIKGK--GLILG 105 Query: 109 LVLIGMLIPNNALMLPIYTIVRKLHILNTHWALIIPYIANAIPFTIIILAAFMRSLPSEI 168 VL + P A++ PIY+ + L + N+++ L+IPYI ++P I ILA F + +P E+ Sbjct: 106 TVLAASMFPQIAIITPIYSFITDLGLRNSYFGLVIPYITISLPLAIWILATFFKKIPFEL 165 Query: 169 EEAAVVDGLKAPGIFARIIVPLTVPAMVTVFIVNFLGNWNEFLLANYFLSNDELRTLPVG 228 EE+A +DG F +II PL P + T I+ F+ WNE+L A + D LRT+PVG Sbjct: 166 EESAKLDGATPFQTFRKIIFPLAAPGVFTTAILVFIAAWNEYLFALTINTADRLRTVPVG 225 Query: 229 MVQFRDQYQMNYAQMSAGIVYSVLPVVIIYAILQEKIIEGVTAGSVK 275 + + QY + + ++A V +P+VII + Q +I+ G+T+GSVK Sbjct: 226 LSLYESQYTIPWGDITAATVIVTVPIVIIVLLFQRRIVAGLTSGSVK 272 >all5282 [G] COG0395 ABC-type sugar transport system, permease component Length = 275 Score = 124 bits (312), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 68/231 (29%), Positives = 127/231 (54%), Gaps = 6/231 (2%) Query: 47 IPKE-FVFNNYVEAWVNAKISTYFFNXXXXXXXXXXXXXXXXXMFAFAVTRMR-QGKWNK 104 +PK + F NY +AW K F N + +A+ R++ +G+ + Sbjct: 48 LPKNNWSFANYRDAWQRGKFLLAFANSTLVAIAVTAFQIVTSALAGYALARLKFRGR--Q 105 Query: 105 ILFSLVLIGMLIPNNALMLPIYTIVRKLHILNTHWALIIPYIANAIPFTIIILAAFMRSL 164 L +VL ++IP L++PI+ +++ H++NT+ ALI+P N F I +L + +++ Sbjct: 106 TLLLIVLATLIIPFQLLVIPIFLVLKWGHLINTYGALILPTAVNG--FGIFLLRQYFQTI 163 Query: 165 PSEIEEAAVVDGLKAPGIFARIIVPLTVPAMVTVFIVNFLGNWNEFLLANYFLSNDELRT 224 P E+EEAA +DG I ++++PL PA+VT+F+ F+ WN+ F + ELRT Sbjct: 164 PVELEEAATIDGANRLQILWQVMLPLARPALVTLFLFTFIAEWNDLFKPLVFTTKPELRT 223 Query: 225 LPVGMVQFRDQYQMNYAQMSAGIVYSVLPVVIIYAILQEKIIEGVTAGSVK 275 + + + +F++Q+ N+ M A + + +PV++++ I Q + I G+ +K Sbjct: 224 VQLALAEFQEQFTNNWPLMMAAVTIATVPVMVLFLIGQRQFIRGIATTGIK 274 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,965,073 Number of extensions: 585810 Number of successful extensions: 2125 Number of sequences better than 1.0e-05: 164 Number of HSP's gapped: 1966 Number of HSP's successfully gapped: 164 Length of query: 276 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 171 Effective length of database: 26,268,361 Effective search space: 4491889731 Effective search space used: 4491889731 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)