BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0036.p (873 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1675_2 [C] COG1454 Alcohol dehydrogenase, class IV 568 e-161 lin1675_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases 565 e-160 SP2026_2 [C] COG1454 Alcohol dehydrogenase, class IV 528 e-149 SP2026_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases 520 e-147 SA0143_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases 503 e-142 >lin1675_2 [C] COG1454 Alcohol dehydrogenase, class IV Length = 411 Score = 568 bits (1464), Expect = e-161, Method: Compositional matrix adjust. Identities = 277/411 (67%), Positives = 323/411 (78%), Gaps = 2/411 (0%) Query: 459 NMQWFKVPDKIYFEKGATQYLAKMPDITRVAIITDPMMVKLGYVERVEHYLRQRQTPVAI 518 NMQWFK+P KI+FEK +TQYL KM + RV I+TDP MV+ YV+ V +L++R VA Sbjct: 2 NMQWFKLPPKIFFEKYSTQYLQKMEGVERVFIVTDPGMVQFKYVDVVIEHLKKRGNDVAY 61 Query: 519 EVFSEVEPDPSTTTVEKGTAMMNRFQPDCIIALGGGSPMDAAKGMWLFYEHPDADFNGLK 578 +VF++VEPDPS TV KG +M F+PD IIALGGGS MDAAKGMWLFYEHP+A F GLK Sbjct: 62 QVFADVEPDPSDVTVYKGAELMKDFKPDTIIALGGGSAMDAAKGMWLFYEHPEASFFGLK 121 Query: 579 QKFMDIRKRVYKFPKLGNKAKFVAIPTTSGTGSEVTSFAVITDKTTANNTKYPLADYELT 638 QKF+DIRKR +K+PKLG KAKFVAIPTTSGTGSEVT FAVITDK NN KYPLADYELT Sbjct: 122 QKFLDIRKRTFKYPKLGGKAKFVAIPTTSGTGSEVTPFAVITDKE--NNIKYPLADYELT 179 Query: 639 PDVAIIDPEFVYSLPKTAVADTGMDVLTHAIEAYVSVMASDYTDGLAIKAIQLVFQYLEK 698 PDVAI+D ++V ++P ADTGMDVLTHAIE+YVSVMASDYT GL+I+AI+LVF+ L + Sbjct: 180 PDVAIVDAQYVTTVPAHITADTGMDVLTHAIESYVSVMASDYTRGLSIRAIELVFENLRE 239 Query: 699 SALQGDKLAREKMHNASTLAGMAFANAFLGINHSLAHKWGGQYHTAHGRTNAILMPHVIR 758 S L GD AREKMHNAS LAGMAFANAFLGINHSLAHK G ++H HGR NAILMPHVIR Sbjct: 240 SVLTGDPDAREKMHNASALAGMAFANAFLGINHSLAHKIGPEFHIPHGRANAILMPHVIR 299 Query: 759 YNAKKPTKFASFPKYSHFVADERYAEIARILGLPARTTEEGVTSLINAIRDMNKKLGIEE 818 YNA KP K A FP+Y F ADE YA I+RI+G PA TTEEGV SL++ I + K +GI+ Sbjct: 300 YNALKPKKHALFPRYESFRADEDYARISRIIGFPAATTEEGVKSLVDEIIKLGKDVGIDM 359 Query: 819 SFQALGFDPKDFESRVDYLADRAFEDQCTTANPKLPLVSELADVYRNAFYG 869 S + KD ++ VD LADRAF DQCTTANPK PLVSEL ++Y A+ G Sbjct: 360 SLKGQNVAKKDLDAVVDTLADRAFMDQCTTANPKQPLVSELKEIYLEAYKG 410 >lin1675_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 455 Score = 565 bits (1456), Expect = e-160, Method: Compositional matrix adjust. Identities = 277/456 (60%), Positives = 350/456 (76%), Gaps = 2/456 (0%) Query: 1 MAVKNEVAAQVKQTTAEEYIQTLVDKAKKAQEAYMQLDQEQVNTIVHAMALAGLDKHMYL 60 MA+K A +V + ++ I L D +KA +A+ +QEQV+ IVHAMALAGLD+HM L Sbjct: 1 MAIKENAAQEVLEV--QKVIDRLADNGQKALKAFESYNQEQVDNIVHAMALAGLDQHMPL 58 Query: 61 AKLAVEETGRGVYEDKITKNIFATEYIWHGIKYDKTVGVVEDNQYDSFQKIAEPVGIVMG 120 AKLAVEETGRG+YEDK KNIFATEYIW+ IK +KTVGV+ ++ +IAEPVG+V G Sbjct: 59 AKLAVEETGRGLYEDKCIKNIFATEYIWNNIKNNKTVGVINEDTQTGVIEIAEPVGVVAG 118 Query: 121 ITPVTNPTSTTMFKALIAAKTRNPIIFGFHPSAQECSAAAAKILHDAAVKAGAPENCIQW 180 +TPVTNPTSTT+FKA+IA KTRNPIIF FHPSAQ CS+ AAK+++DAAV AGAPE+CIQW Sbjct: 119 VTPVTNPTSTTLFKAIIAIKTRNPIIFAFHPSAQRCSSEAAKVVYDAAVAAGAPEHCIQW 178 Query: 181 IELPTMDKTNALMNNPDVALILATGGSAMVKAAYSCGKPALGVGPGNVPAFIEKSADIDQ 240 +E P+++ T LMN+ VAL+LATGG+ MVK+AYS GKPALGVGPGNVPA+I+K+A I + Sbjct: 179 VEKPSLEATKQLMNHDKVALVLATGGAGMVKSAYSTGKPALGVGPGNVPAYIDKTAKIKR 238 Query: 241 AVTDIILSKTFDNGMICASEQAVIIEEAIFDQVKKKMIANGCYFVNKEEAAKLTSGAMNV 300 +V DIILSK+FD GMICASEQAVI+++ + +VK +M AN CYFV E KL S +N Sbjct: 239 SVNDIILSKSFDQGMICASEQAVIVDKEVAKEVKAEMEANKCYFVKGAEFKKLESYVINP 298 Query: 301 EKCAVNPAIVGQSAVKIAEMCGIQVPAGTKILVAELEGVGTKYPLSAEKLSPVLACYKVK 360 EK +NP +VG+S IA G +VP TKILVAE++GVG KYPLS EKLSPVLA + Sbjct: 299 EKGTLNPDVVGKSPAWIANQAGFKVPEDTKILVAEIKGVGDKYPLSHEKLSPVLAFIEAA 358 Query: 361 NADQGIERAAQVVEFGGMGHSSAIHSHNEEVIMKFSNRLQTGRILVNSPSTHGAIGDIYN 420 N + +R +++ +GG+GHS+ IHS ++EV F R++ RI+VN+PS G IGDIYN Sbjct: 359 NQAEAFDRCEEMLVYGGLGHSAVIHSTDKEVQKAFGIRMKACRIIVNAPSAQGGIGDIYN 418 Query: 421 TNIPSLTLGCGSYGRNSVSQNVTAVNLINVKRVNRR 456 IPSLTLGCGSYG+NSVSQNV+A NL+NVKR+ R Sbjct: 419 GFIPSLTLGCGSYGKNSVSQNVSATNLLNVKRIADR 454 >SP2026_2 [C] COG1454 Alcohol dehydrogenase, class IV Length = 419 Score = 528 bits (1361), Expect = e-149, Method: Compositional matrix adjust. Identities = 248/411 (60%), Positives = 315/411 (76%), Gaps = 2/411 (0%) Query: 459 NMQWFKVPDKIYFEKGATQYLAKMPDITRVAIITDPMMVKLGYVERVEHYLRQRQTPVAI 518 NMQW K+P K YFE+ + QYL K D+ RV I+TD MV+LG+++R+ L R+ V Sbjct: 2 NMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDRIIEQLDLRRNKVVY 61 Query: 519 EVFSEVEPDPSTTTVEKGTAMMNRFQPDCIIALGGGSPMDAAKGMWLFYEHPDADFNGLK 578 ++F++VEPDP TTV +GT +M F+PD IIALGGGSPMDAAK MWLFYE P+ DF L Sbjct: 62 QIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDAAKVMWLFYEQPEVDFRDLV 121 Query: 579 QKFMDIRKRVYKFPKLGNKAKFVAIPTTSGTGSEVTSFAVITDKTTANNTKYPLADYELT 638 QKFMDIRKR +KFP LG K KF+AIPTTSGTGSEVT FAVI+DK ANN KYP+ADY LT Sbjct: 122 QKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFAVISDK--ANNRKYPIADYSLT 179 Query: 639 PDVAIIDPEFVYSLPKTAVADTGMDVLTHAIEAYVSVMASDYTDGLAIKAIQLVFQYLEK 698 P VAI+DP V ++P ADTGMDVLTHA EAYVS MASDYTDGLA++AI+LVF+ LE Sbjct: 180 PTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMASDYTDGLALQAIKLVFENLES 239 Query: 699 SALQGDKLAREKMHNASTLAGMAFANAFLGINHSLAHKWGGQYHTAHGRTNAILMPHVIR 758 S D +REKMHNAST+AGMAFANAFLGI+HS+AHK G Q+HT HGRTNAIL+P+VIR Sbjct: 240 SVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAHKIGAQFHTIHGRTNAILLPYVIR 299 Query: 759 YNAKKPTKFASFPKYSHFVADERYAEIARILGLPARTTEEGVTSLINAIRDMNKKLGIEE 818 YN +P K A++PKY+++ ADE+Y +IAR+LGLPA T EEGV S A+ ++ +++GI+ Sbjct: 300 YNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPASTPEEGVESYAKAVYELGERIGIQM 359 Query: 819 SFQALGFDPKDFESRVDYLADRAFEDQCTTANPKLPLVSELADVYRNAFYG 869 +F+ G D K+++ LA A+EDQC+ ANP+LP+V + ++ +A+YG Sbjct: 360 NFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANPRLPMVDHMQEIIEDAYYG 410 >SP2026_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 464 Score = 520 bits (1338), Expect = e-147, Method: Compositional matrix adjust. Identities = 261/463 (56%), Positives = 337/463 (72%), Gaps = 7/463 (1%) Query: 1 MAVKNEVAAQVKQTTAEEYIQTLVDKAKKAQEAYMQLDQEQVNTIVHAMALAGLDKHMYL 60 MA K V + K+ AE+++ LV KA A E +LDQEQV+ IV ++A LD H L Sbjct: 1 MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDYIVAKASVAALDAHGEL 60 Query: 61 AKLAVEETGRGVYEDKITKNIFATEYIWHGIKYDKTVGVVEDNQYDSFQKIAEPVGIVMG 120 A A EETGRGV+EDK TKN+FA E++ + +++ KTVGV+E++ IAEPVG+V G Sbjct: 61 ALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDVTGLTLIAEPVGVVCG 120 Query: 121 ITPVTNPTSTTMFKALIAAKTRNPIIFGFHPSAQECSAAAAKILHDAAVKAGAPENCIQW 180 ITP TNPTST +FK+LI+ KTRNPI+F FHPSAQE SA AA+I+ DAA+ AGAPENC+QW Sbjct: 121 ITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHAARIVRDAAIAAGAPENCVQW 180 Query: 181 IELPTMDKTNALMNNPDVALILATGGSAMVKAAYSCGKPALGVGPGNVPAFIEKSADIDQ 240 I P+M+ T+ALMN+ VA ILATGG+AMVKAAYSCGKPALGVG GNVPA++EKSA+I Q Sbjct: 181 ITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGKPALGVGAGNVPAYVEKSANIRQ 240 Query: 241 AVTDIILSKTFDNGMICASEQAVIIEEAIFDQVKKKMIANGCYFVNKEEAAKLTSGAM-- 298 A DI++SK+FDNGM+CASEQAVII++ I+D+ + + YFVNK+E A L Sbjct: 241 AAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAEFKSYHTYFVNKKEKALLEEFCFGV 300 Query: 299 --NVEKCA---VNPAIVGQSAVKIAEMCGIQVPAGTKILVAELEGVGTKYPLSAEKLSPV 353 N + CA +N IVG+ A IAE G VP GT IL AE + VG PL+ EKLSPV Sbjct: 301 KANSKNCAGAKLNADIVGKPATWIAEQAGFTVPEGTNILAAECKEVGENEPLTREKLSPV 360 Query: 354 LACYKVKNADQGIERAAQVVEFGGMGHSSAIHSHNEEVIMKFSNRLQTGRILVNSPSTHG 413 +A K ++ + GI +A Q+VEF G+GHS+AIH+ +EE+ +F ++ R++ NSPST G Sbjct: 361 IAVLKSESREDGITKARQMVEFNGLGHSAAIHTADEELTKEFGKAVKAIRVICNSPSTFG 420 Query: 414 AIGDIYNTNIPSLTLGCGSYGRNSVSQNVTAVNLINVKRVNRR 456 IGD+YN +PSLTLGCGSYGRNSV NV+A+NL+N+K+V RR Sbjct: 421 GIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAINLLNIKKVGRR 463 >SA0143_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Length = 458 Score = 503 bits (1295), Expect = e-142, Method: Compositional matrix adjust. Identities = 246/437 (56%), Positives = 331/437 (75%) Query: 20 IQTLVDKAKKAQEAYMQLDQEQVNTIVHAMALAGLDKHMYLAKLAVEETGRGVYEDKITK 79 I L DK KKA EA + QE+++ IVH M+LA +D+HM LAKLA EETGRG+YEDK K Sbjct: 21 IDALADKGKKALEALSKKSQEEIDHIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIK 80 Query: 80 NIFATEYIWHGIKYDKTVGVVEDNQYDSFQKIAEPVGIVMGITPVTNPTSTTMFKALIAA 139 N++A+EYIW+ IK +KTVG++ +++ +AEP+G++ G+TP TNPTSTT+FKA+IA Sbjct: 81 NLYASEYIWNSIKDNKTVGIIGEDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAI 140 Query: 140 KTRNPIIFGFHPSAQECSAAAAKILHDAAVKAGAPENCIQWIELPTMDKTNALMNNPDVA 199 KT NPIIF FHPSAQE S AA+++ +AA+KAGAP++ IQWIE+P+++ T LMN+ +A Sbjct: 141 KTGNPIIFAFHPSAQESSKRAAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIA 200 Query: 200 LILATGGSAMVKAAYSCGKPALGVGPGNVPAFIEKSADIDQAVTDIILSKTFDNGMICAS 259 L+LATGGS MVK+AYS GKPALGVGPGNVP++IEK+A I +AV DII SKTFDNGMICAS Sbjct: 201 LVLATGGSGMVKSAYSTGKPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICAS 260 Query: 260 EQAVIIEEAIFDQVKKKMIANGCYFVNKEEAAKLTSGAMNVEKCAVNPAIVGQSAVKIAE 319 EQ V+I++ I+ V + A+ YFV K+E +L + MN +K + P IVG+SAV+IAE Sbjct: 261 EQVVVIDKEIYKDVTNEFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAE 320 Query: 320 MCGIQVPAGTKILVAELEGVGTKYPLSAEKLSPVLACYKVKNADQGIERAAQVVEFGGMG 379 + GI VP TK+++AE+ GVG+ YPLS EKLSPVLA K ++ Q + + FGG+G Sbjct: 321 LAGIPVPENTKLIIAEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLG 380 Query: 380 HSSAIHSHNEEVIMKFSNRLQTGRILVNSPSTHGAIGDIYNTNIPSLTLGCGSYGRNSVS 439 H++ IH+ +E + F R++ R+LVN+PS G IGD+YN IPSLTLGCGSYGRNS+S Sbjct: 381 HTAVIHTEDETLQKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSIS 440 Query: 440 QNVTAVNLINVKRVNRR 456 NV+A +L+N+K + +R Sbjct: 441 HNVSATDLLNIKTIAKR 457 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 62,897,263 Number of extensions: 2649610 Number of successful extensions: 6293 Number of sequences better than 1.0e-05: 172 Number of HSP's gapped: 5986 Number of HSP's successfully gapped: 172 Length of query: 873 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 758 Effective length of database: 25,115,021 Effective search space: 19037185918 Effective search space used: 19037185918 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)