BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0033.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic... 69 9e-12 SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmi... 57 4e-08 AGl1009 [G] COG1653 ABC-type sugar transport system, periplasmic... 55 1e-07 PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic ... 55 1e-07 >lin0220 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 418 Score = 68.9 bits (167), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 65/229 (28%), Positives = 99/229 (43%), Gaps = 12/229 (5%) Query: 6 DDKIKRFQAEYPNVEIVKDDWQYN--VSEIGVKMAANEAPTFFN-TYATEAKFLVEKGWV 62 D +K F+ + P++++ +N +++ ++A +AP F Y T ++ EKG + Sbjct: 48 DKMVKEFEKQNPDIKVNVQTIAFNDYFTKLQTQIAGGDAPDAFELNYETFMQY-AEKGVL 106 Query: 63 ADITGLWNKYEYKD--QINPVLQNQFIIDGKVYGITQKGYVTTTMLNKKMLDDKGVAVPP 120 AD+T K + D +N + F DGK YG+ + T+ NK + D GV P Sbjct: 107 ADLTSYIEKDKDFDPSTLNKQAYDAFKYDGKQYGMVESFSNVVTIYNKDLFDKAGVEYPT 166 Query: 121 MDWTWEDMLNTAKGAADAKKGISGIAPMGKGNEAGWNWTNFLFEAGGEIQKVDGGKVVAT 180 DWTW+D AK DAK + G + NE + + GG I D K T Sbjct: 167 ADWTWKDEEAAAKKLTDAKNKVWGTSQPVTMNE----FFKVAAQNGGSIFDKDMTKT--T 220 Query: 181 FNSEAGVKALDFYKKLRWEANAIPQDWALGWGDAVGAFQQGRTAMVMAG 229 NS V+AL E+ P L F G+ AM+ G Sbjct: 221 INSPENVEALTHLTNEVTESKVAPSPSDLSGQLPEDLFMNGQIAMLHTG 269 >SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 451 Score = 56.6 bits (135), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 67/282 (23%), Positives = 109/282 (38%), Gaps = 16/282 (5%) Query: 6 DDKIKRFQAEY-PNVEIVKDDWQYNVSEIGVKMAANEAPTFFNTYATEAKFLVEKGWVAD 64 D ++ + E+ PN E + ++ A+ E P F + G + D Sbjct: 76 DSHPQKIELEFIPNSEYISG------PKLATAFASGEGPDIFIISPGDFLRYYNGGVLKD 129 Query: 65 ITGLWNKYEYKDQINPVLQNQFIIDGKVYGITQKGYVTTTMLNKKMLDDKGVAVPPMDWT 124 +T + D V+ N+ ++DGK++GI + + K ++ G+ + T Sbjct: 130 LTPFIDDKAKADFPESVIANR-MVDGKIFGIPMEVEPMAMYYSVKAFEEAGLNENDVPKT 188 Query: 125 WEDMLNTAKGAADAKKGISGIAPMGKGNEAGWNWTNFLFEAGGEIQKVDGGKVVATFNSE 184 WE +L AK ++ + G + W FL++ GGE Q DG ++F+S Sbjct: 189 WEALLEVAKKLTTPER-FGVMFETQPGYYQNFTWYPFLWQGGGEFQTADGK---SSFDSP 244 Query: 185 AGVKALDFYKKLRWEANAIPQDWALGWGDAVGAFQQGRTAMVMAGSDGVIEQALNQGGLK 244 A V+AL F++ A Q G D + G AM G G+ + N + Sbjct: 245 ATVQALKFWQDAINSGVAPRQIMGAGGHDTIANLASGYVAMQNVGIWGISQLQSNAKDFQ 304 Query: 245 PEDVLTYPMPAAEKGGKHTGVLGGDYLVXXXECHPGRAGDGF 286 V P P GK+ V GG V H A F Sbjct: 305 -YGVFRLPTP---PNGKYVTVGGGWAFVANANGHNSDAAGEF 342 >AGl1009 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 134 Score = 55.5 bits (132), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 0/285 (0%), Positives = 0/285 (0%), Gaps = 29/285 (10%) Query: 9 IKRFQAEYPNVEIVKDDWQYN--VSEIGVKMAANEAPTFFNTYATEAKFLVEKGWVADIT 66 + A + ++ + T + + + + A Sbjct: 47 KAKAPAVLQKLAQAVAAEGFSGIQINKALSSIDAHQETSGSGRIQTLRVVARQKGAAVRI 106 Query: 67 GLWNKYEYKDQIN--PVLQNQFIIDGKVYGITQKGYVTTTMLNKKMLDDKGV--AVPPMD 122 + + Sbjct: 107 DAVFNIQAGQIADKD- 165 Query: 123 WTWEDMLNTAKGAADAKKGISGIAPMGKGNEAGWNWTNFLFEAGGEIQKVDGGKVVATFN 182 Sbjct: 166 --------- 216 Query: 183 SEAGVKALDFYKKLRWEANAIPQDWALGWGDAVGAFQQGRTAMVMAGS---DGVIEQALN 239 Sbjct: 217 276 Query: 240 QGGLKPEDVLTYPMPAAEKGGKHTGVLGGDY--LVXXXECHPGRA 282 Sbjct: 277 -------- 313 >PH0753 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 464 Score = 55.5 bits (132), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 78/297 (26%), Positives = 121/297 (40%), Gaps = 42/297 (14%) Query: 9 IKRFQAEYPNV----EIVKDDWQYNVSEIGVKMAANEAPTFFNTYATEAKFLVEKGWVAD 64 I+ F+ E PN+ E++ + N I A AP F ++ A +EKG + Sbjct: 89 IELFEKENPNIKVKYEVITQMFHEN---ILASFGAGVAPDVFYVDSSWAPIFIEKGALYP 145 Query: 65 ITGLWNKYEYKDQINPVLQNQFIIDGKVYGITQKGYVTTTMLNKKMLDDKGVAVPPMDWT 124 I+ L ++ + ++ P L F +GK+YG+ + + NKK+ + G+ PP T Sbjct: 146 ISELADE-SFINKFYPFLLEPFKKNGKLYGLPKDWSMLALFYNKKLFEQAGLTKPPE--T 202 Query: 125 WEDMLNTAK---------GAADAKKGISGIAPMGKGNEAGWNWTNFLFEAGGEIQKVDGG 175 W+++L AK G A G + P+ N A W FE + D Sbjct: 203 WDELLEYAKIITEKTGKPGLAIYLGGFNRYVPVAVSNGAPKPW----FEKPEDASWFDNP 258 Query: 176 KVVATFNSEAGVKALDFYKKLRWEAN--------AIPQDWALGW-GDAVGAFQQGRTAMV 226 V T ++ Y+K + E P+D GW GDA G Q + AMV Sbjct: 259 VVRETLTW-----YINLYRKGKVEREKKGLPPYVVAPEDVGAGWLGDAFG---QQKVAMV 310 Query: 227 MAGSDGVIEQALNQGGLKP-EDVLTYPMPAAEKGGKHTGVLGGDYLVXXXECHPGRA 282 ++G+ + A K ED P+PA K G+ T V + HP A Sbjct: 311 ISGNWMIPFLADQFPNFKYGEDWDIAPLPAG-KNGRVTMVYTVILGINSKTKHPKEA 366 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.136 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,027,611 Number of extensions: 1033796 Number of successful extensions: 1771 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 1782 Number of HSP's successfully gapped: 4 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)