BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0030.p (307 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0760 [G] COG1175 ABC-type sugar transport systems, permease c... 169 7e-42 YPO3471 [G] COG1175 ABC-type sugar transport systems, permease c... 159 7e-39 BH1865 [G] COG1175 ABC-type sugar transport systems, permease co... 151 1e-36 alr0789 [G] COG1175 ABC-type sugar transport systems, permease c... 150 2e-36 BS_yurN [G] COG1175 ABC-type sugar transport systems, permease c... 139 5e-33 >lin0760 [G] COG1175 ABC-type sugar transport systems, permease components Length = 296 Score = 169 bits (427), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 94/277 (33%), Positives = 147/277 (53%), Gaps = 1/277 (0%) Query: 31 GYMFLLPAIIIFVLFMWVPIFKGFMYSFYTVDFVKGNTFAGLDNYARALTDPDVLIAVKN 90 Y+F+ +++ +FM +PI SF D V + G DNY + DP LI++KN Sbjct: 17 AYLFIALPVLLLAIFMILPIGMALFVSFTDYDIVNSPNWIGFDNYIKMFRDPYFLISLKN 76 Query: 91 TLYYMVLCLVIGFWVPIAFAIAISELRKFGGFVRVAAYLPFVLPAVVLYGLWRWLYDP-V 149 T+ Y L + +G + AI I+ +K G R Y+P + V +W WL +P Sbjct: 77 TIIYTALFVPLGLVASLGAAILINMNKKGAGLFRTFFYVPVLCSTVATATIWYWLLNPQY 136 Query: 150 GPINALLGTMGADQISFLTDSRWSMISLVFMETWQQFGSGMLIYLAAVLSIPRDWYEAAE 209 G IN +LG G + ++L DS W+M ++V M W FG+ M+I+LA + IP + YEA++ Sbjct: 137 GLINRVLGWFGINGPAWLYDSNWAMFAIVMMSVWMTFGTNMMIFLAGLQGIPANLYEASK 196 Query: 210 IDGAGVWARIRHITLPSLRNLIVLMLILQIIATSQGYQSQLALLDGGPNNTTLTYALLIV 269 I+GA W R++T PSL L+ + II Q + L GGP N+T+T I Sbjct: 197 IEGASRWKTFRYVTWPSLSRTTFLVTTMLIINAFQVFDQAYVLTKGGPGNSTITLVYYIY 256 Query: 270 KYAFTRLDYGTATALGMLMFIVLGGLGIVQFKLNKED 306 F L G A+A+ ++F+++ I+ ++NKED Sbjct: 257 NEGFGGLKMGYASAISFVLFLIILVFSIINMRVNKED 293 >YPO3471 [G] COG1175 ABC-type sugar transport systems, permease components Length = 294 Score = 159 bits (401), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 89/284 (31%), Positives = 156/284 (54%), Gaps = 2/284 (0%) Query: 24 RWKKNFWGYMFLLPAIIIFVLFMWVPIFKGFMYSFYTVDFVKGNTFAGLDNYARALTDPD 83 R K+N ++FL PA++IF F ++P+ +G + SFY V+F + N + G N++RA D Sbjct: 7 RLKRNLVPWLFLAPALVIFTWFKFIPMLQGLVMSFYKVNFNQPNEWVGFANFSRAFGDEA 66 Query: 84 VLIAVKNTLYYMVLCLVIGFWVPIAFAIAISELRKFGGFVRVAAYLPFVLPAVVLYGLWR 143 + AV NTL Y+++ +V+ ++ A+ + + F+R A +LP V A ++ +WR Sbjct: 67 LHSAVFNTLLYVLVTMVVAAFIAFFLAMLLEGPARHLRFIRTAIFLPAVTSAAIVAEMWR 126 Query: 144 WLYDPV--GPINALLGTMGADQISFLTDSRWSMISLVFMETWQQFGSGMLIYLAAVLSIP 201 L++P G +N +L + FL D+ ++ +++ + W+ M+I++A ++ I Sbjct: 127 ILFNPTSNGVVNHVLSWFSIEAQGFLADTDQALWTVMLLHIWKAVPYNMVIFIAGLVGIS 186 Query: 202 RDWYEAAEIDGAGVWARIRHITLPSLRNLIVLMLILQIIATSQGYQSQLALLDGGPNNTT 261 RD Y+A+ +DGA W R+R++TLP + + ++L+L I + + A GGP+N T Sbjct: 187 RDLYDASNVDGANWWNRLRYVTLPGMIPALSVVLMLSFIRGFRVFAEVYATTGGGPSNAT 246 Query: 262 LTYALLIVKYAFTRLDYGTATALGMLMFIVLGGLGIVQFKLNKE 305 I K F + DYG A+A+ L+F L I L K Sbjct: 247 EMIMTHIYKLGFEQFDYGYASAVSFLLFAFTVVLTIGHLTLKKR 290 >BH1865 [G] COG1175 ABC-type sugar transport systems, permease components Length = 309 Score = 151 bits (381), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 93/304 (30%), Positives = 153/304 (50%), Gaps = 3/304 (0%) Query: 3 QTASIHETMPAKLERKPHKQNRWKKNFWGYMFLLPAIIIFVLFMWVPIFKGFMYSFYTVD 62 + + ET K ++ + N K+ FWGY+F+ P II F +F P+ SF D Sbjct: 4 EVETPRETKTTKARKQKRRLN--KEYFWGYLFIAPPIIGFAIFALGPMLYSIYVSFTDFD 61 Query: 63 FVKGNTFAGLDNYARALTDPDVLIAVKNTLYYMVLCLVIGFWVPIAFAIAISELRKFGGF 122 + G DNY R D+ +Y L + IG V + A+A+++ K Sbjct: 62 LYNEPVWTGADNYYRLFVTDDLFRKTVFNTFYAALGIPIGMAVSLGIAVALNQKVKGIAL 121 Query: 123 VRVAAYLPFVLPAVVLYGLWRWLYDP-VGPINALLGTMGADQISFLTDSRWSMISLVFME 181 R A +LP V V + LWRW+++ G +N +L +G +L+D +W+M +++ Sbjct: 122 FRTAFFLPAVSSVVAITLLWRWIFNADFGLLNIMLNYVGIHGPGWLSDEKWAMPAMIIQG 181 Query: 182 TWQQFGSGMLIYLAAVLSIPRDWYEAAEIDGAGVWARIRHITLPSLRNLIVLMLILQIIA 241 W G M++YLAA+ + YEAA+IDG W + HIT+PS+ +LI I Sbjct: 182 VWGGLGINMILYLAALQGVNPALYEAADIDGGNAWQKFIHITVPSISPTTFFILITSTIG 241 Query: 242 TSQGYQSQLALLDGGPNNTTLTYALLIVKYAFTRLDYGTATALGMLMFIVLGGLGIVQFK 301 Q +Q + + +GGPN +T T + AF ++ G A+A+ ++ I++ + I+ FK Sbjct: 242 ALQDFQRFMIMTEGGPNYSTTTVVYYLFLNAFRYMEMGYASAMAWVLGIIILIITIINFK 301 Query: 302 LNKE 305 L K+ Sbjct: 302 LAKK 305 >alr0789 [G] COG1175 ABC-type sugar transport systems, permease components Length = 359 Score = 150 bits (380), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 16/270 (5%), Positives = 39/270 (14%), Gaps = 5/270 (1%) Query: 11 MPAKLERKPHKQNRWKKNFWGYMFLLPAIIIFVLFMWVPIFKGFMYSFYTVDFVKGNTF- 69 M +++ A ++ + G + F Sbjct: 1 MQ-AATVVINRRA---LRHNLQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFG 56 Query: 70 AGLDNYARALTDPDVLIAVKNTLYYMVLCLVIGFWVPIAFAIAISELRKFGGFVRVAAYL 129 A L + V + + +E + Sbjct: 57 VARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEP 116 Query: 130 PFVLPAVVLYGLWRWLYDPVGPINALLGTMGADQISFLTDSRWSMISLVFMETWQQFGSG 189 V + + T + + + + Sbjct: 117 VTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLA 176 Query: 190 MLIYLAAVLSIPRDWYEAAEIDGAGVWARIRHITLPSLRNLIVLMLILQIIATSQGYQSQ 249 + R + I L + L Sbjct: 177 IFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMS 236 Query: 250 LALLDGGPNNTTLTYALLIVKYAFTRLDYG 279 L + + D Sbjct: 237 LTSSLIAVREHKAGEPVGYGGTWVSERDTR 266 >BS_yurN [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 139 bits (351), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 86/284 (30%), Positives = 152/284 (53%), Gaps = 10/284 (3%) Query: 32 YMFLLPAIIIFVLFMWVPIFKGFMYSFYT-VDFVKGNTFAGLDNYARALTDPDVLIAVKN 90 Y+FL+PA++ F+LF+++PIF+ S + F TF GL NY DP A+ N Sbjct: 10 YLFLVPALV-FLLFVYIPIFENVFLSLFQWSSFSPEKTFIGLKNYVELFHDPVFYQALTN 68 Query: 91 TLYYMVLCLVIGFWVPIAFAIAISE--LRKFGGFVRVAAYLPFVLPAVVLYGLWRWLYDP 148 + Y V+ +V + + A + + +RK+ F R +LP V+ V+ L+ ++Y+P Sbjct: 69 NVLYAVISIVCQVFGGLILAAVLEDKLVRKWSPFFRTVFFLPVVISMTVIALLFDFIYNP 128 Query: 149 -VGPINALLGTMGADQIS--FLTDSRWSMISLVFMETWQQFGSGMLIYLAAVLSIPRDWY 205 G +N LL +G DQ++ +L D +M+S++F+ WQ G ++Y+ ++ IP + Y Sbjct: 129 ETGLLNQLLQAIGLDQLTRAWLGDDSTAMLSVIFVSQWQSVGYIAMLYIVSIQKIPDELY 188 Query: 206 EAAEIDGAGVWARIRHITLPSLRNLIVLMLILQIIATSQGYQSQLALLDGGPNNTTLTYA 265 EAA +DGAG + HIT+P + + + +++ + + L GGP + + Sbjct: 189 EAARLDGAGKIQQFFHITVPQTKEMSFVAVVMTLTGAFTVFNEPYILTGGGPGKASEVLS 248 Query: 266 LLIVKYAFTRLDYGTATALGMLMFIVLGGLGIVQ---FKLNKED 306 + K AFT+ G A+A+ ++ I+ L ++Q FK KE+ Sbjct: 249 TFLYKSAFTKDMMGYASAIATVVLIITLALSLMQMKFFKTGKEE 292 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,627,699 Number of extensions: 885310 Number of successful extensions: 3085 Number of sequences better than 1.0e-05: 162 Number of HSP's gapped: 2844 Number of HSP's successfully gapped: 163 Length of query: 307 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 201 Effective length of database: 26,153,027 Effective search space: 5256758427 Effective search space used: 5256758427 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)