BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0027.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CC2313 [I] COG0657 Esterase/lipase 159 5e-39 AGc2981 [I] COG0657 Esterase/lipase 134 1e-31 L42784 [I] COG0657 Esterase/lipase 127 3e-29 CC1226 [I] COG0657 Esterase/lipase 125 5e-29 Rv0220 [I] COG0657 Esterase/lipase 62 6e-10 >CC2313 [I] COG0657 Esterase/lipase Length = 328 Score = 159 bits (401), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 99/264 (37%), Positives = 134/264 (50%), Gaps = 25/264 (9%) Query: 4 KTMLLWPEGAPGALGTSDED--------------------CPAITPYLVEGDKNAAVLVC 43 +TMLLWP GAPG G E+ P + + + AAV++ Sbjct: 41 ETMLLWPNGAPGGDGVDVEEKILERGDPKGLRDRAQVHTRVPKLVVFRPKTPNGAAVMLI 100 Query: 44 PGGGY-GMRADHEGGPVAEWLNTLGISAFVLRYRVAPYGYPHA----LRDAQRALRTIRY 98 PGGGY + D EG A WL+ G + FVL YR+ G+ L+DAQRA+R IR Sbjct: 101 PGGGYERVVLDKEGYETARWLSARGYTCFVLFYRLPGDGWAAGGDTPLQDAQRAVRLIRA 160 Query: 99 RAEEYGIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERKSSRPDLLILCYPVIS 158 +A DR+ ++GFSAGGHLA+ FD + + ++P+L L YPVI+ Sbjct: 161 QAAAMAFSADRVAVMGFSAGGHLAANLTTRFDVATYAPFDAADGLPAKPNLSALIYPVIT 220 Query: 159 MAEGVTHQGSKENQLGTDPDPELVKRLSSELQVTAATPPAFLWHTSDDGAVPVENSLQFA 218 + H GS++ L PE V LS E QV PP F H +DD AVPVENSL Sbjct: 221 LDPAFAHGGSRKQLLARQDTPERVAALSLEKQVREGLPPVFQLHAADDKAVPVENSLLMF 280 Query: 219 AALSRHRIPFDLHVYAHGQHGLGL 242 +AL +P ++H++ G HG GL Sbjct: 281 SALKAKGVPAEMHIFEEGGHGFGL 304 >AGc2981 [I] COG0657 Esterase/lipase Length = 301 Score = 134 bits (338), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/233 (0%), Positives = 0/233 (0%), Gaps = 5/233 (2%) Query: 38 AAVLVCPGGGYG-MRADHEGGPVAEWLNTLGISAFVLRYRVAPYGY---PHA-LRDAQRA 92 + G + W + LR AP +++ Sbjct: 76 RLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVILAVNKVDNVQEKADL 135 Query: 93 LRTIRYRAEEYGIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERKSSRPDLLIL 152 L +++ A + +A + Sbjct: 136 LPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMAS 195 Query: 153 CYPVISMAEGVTHQGSKENQLGTDPDPELVKRLSSELQVTAATPPAFLWHTSDDGAVPVE 212 + + + + + + + + ++ Sbjct: 196 EIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIK 255 Query: 213 NSLQFAAALSRHRIPFDLHVYAHGQHGLGLADGEPHTRSWTEACASWLALNGY 265 Sbjct: 256 308 >L42784 [I] COG0657 Esterase/lipase Length = 268 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 77/236 (32%), Positives = 114/236 (48%), Gaps = 32/236 (13%) Query: 39 AVLVCPGGGYGMRADHEGGPVAEWLNTLGISAFVLRYRVAPYGYPHA-----LRDAQRAL 93 A+++CPGGGY + E P+A G +L Y V G + L + Q Sbjct: 45 AIIICPGGGYQHISQRESDPLALAFLAQGYQVLLLNYTVMNKGTNYNFLSQNLEEVQAVF 104 Query: 94 RTIRYRAEEYGIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERKSSRPDLLILC 153 I +E+ I+P+++ +LG SAGGHLA+ G SE + R P +ILC Sbjct: 105 SLIHQNHKEWQINPEQVFLLGCSAGGHLAAWYG---------NSEQIHR----PKGVILC 151 Query: 154 YPVISMAEGVTHQGSKENQLGTDPDPELVKRLSSELQVTAATPPAFLWHTSDDGAVPVEN 213 YPV S G S N + E + + +VT++TPP F+WHT+DD VP+ N Sbjct: 152 YPVTSFTFGWPSDLSHFNF-----EIENISEYNISEKVTSSTPPTFIWHTADDEGVPIYN 206 Query: 214 SLQFAAALSRHRIPFDLHVYAHGQHGLGLADGE---------PHTRSWTEACASWL 260 SL++ LS+H++PF+ H + G HG+ LA+ P W + WL Sbjct: 207 SLKYCDRLSKHQVPFEAHFFESGPHGVSLANRTTAPSDAYCLPSVHRWVSWASDWL 262 >CC1226 [I] COG0657 Esterase/lipase Length = 278 Score = 125 bits (315), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 94/289 (32%), Positives = 143/289 (49%), Gaps = 57/289 (19%) Query: 4 KTMLLWPEGAPGALG------------TSDEDCPAITPYL--VEGDKNAAVLVCPGGGYG 49 + + LWP GAPG + P+IT YL E AA++V PGGG+ Sbjct: 20 QQLPLWPNGAPGFESRKAIPEQAADYWVKSVNNPSITAYLPPKETATGAALIVLPGGGHR 79 Query: 50 MRADH-EGGPVAEWLNTLGISAFVLRYRV-----APYGYPHALRDAQRALRTIRYRAEEY 103 + H EG VA+ +N G++ FV++YR+ +PY HA +DA RA+R +R A + Sbjct: 80 ILVIHSEGSDVAKVMNPKGVAVFVVKYRLFREEGSPYTIDHARQDAVRAVRYVRAHAAVF 139 Query: 104 GIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERKSSRPDLLILCYPVISMAEGV 163 G+DP R+G++GFSAGG + A V A ++ ++ ++ S+RPD +L YP Sbjct: 140 GVDPKRIGLMGFSAGGEAVNLA-VYQGAANDPKTDAVDAVSARPDFQVLVYP-------- 190 Query: 164 THQGSKENQLGTDPDPELVKRLSSELQVTAATPPAFLWHTSDD---GAVPVENSLQFAAA 220 LG PE +V TPPAF+ +DD A PV+ +F AA Sbjct: 191 -------GPLGV---PE---------KVPTDTPPAFIVAANDDECCSAPPVDLLTRFRAA 231 Query: 221 LSRHRIPFDLHVYAHGQH--GLGLADGEPHTRSWTEACASWLALNGYTK 267 ++P + H++ G H +GL + ++W + WLA NG+ K Sbjct: 232 ----KVPIEFHLFQSGGHAFNMGLRSHKAALKAWPDRLFDWLADNGWLK 276 >Rv0220 [I] COG0657 Esterase/lipase Length = 403 Score = 62.4 bits (150), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 66/225 (29%), Positives = 104/225 (46%), Gaps = 19/225 (8%) Query: 36 KNAAVLV-CPGGGY--GMRADHEGGPVAEWLNTLGISAFVLRYRVAPY-GYPHALRDAQR 91 K A VL+ PGG + G RA +G V L G + YRVAP+ +P + D + Sbjct: 154 KPAPVLIFVPGGAWVHGSRAI-QGYAVLSRLAAQGWVCLSIDYRVAPHHRWPRHILDVKT 212 Query: 92 ALRTIRYRAEEYGIDPDRLGILGFSAGGHLASAAGVLFDAGDEEASEPLERKSSRPDLLI 151 A+ R +++G D + + + G SAGGHL++ AG+ + +A P E + D ++ Sbjct: 213 AIAWARANVDKFGGDRNFIAVAGCSAGGHLSALAGLTANDPQYQAELP-EGSDTSVDAVV 271 Query: 152 LCYPVISMAEGVTHQGSK--------ENQLGTDPDPELVKRLSSELQVTAATPPAFLWHT 203 Y + T + ++ Q D PE+ + S +VT PP + H Sbjct: 272 GIYGRYDWEDRSTPERARFVDFLERVVVQRTIDRHPEVFRDASPIQRVTRNAPPFLVIHG 331 Query: 204 SDDGAVPVENSLQFAA---ALSRHRIPFDLHVYAHGQHGLGLADG 245 S D +PVE + F A+SR ++ + L + G HG L DG Sbjct: 332 SRDCVIPVEQARSFVERLRAVSRSQVGY-LELPGAG-HGFDLLDG 374 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,897,030 Number of extensions: 1015352 Number of successful extensions: 2672 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2662 Number of HSP's successfully gapped: 15 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)