BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0024.p (479 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA2098 [G] COG1554 Trehalose and maltose hydrolases (possible p... 372 e-102 L128690 [G] COG1554 Trehalose and maltose hydrolases (possible p... 367 e-101 lin2226 [G] COG1554 Trehalose and maltose hydrolases (possible p... 342 9e-94 all4989 [G] COG1554 Trehalose and maltose hydrolases (possible p... 259 5e-69 Rv3401 [G] COG1554 Trehalose and maltose hydrolases (possible ph... 253 4e-67 >NMA2098 [G] COG1554 Trehalose and maltose hydrolases (possible phosphorylases) Length = 752 Score = 372 bits (954), Expect = e-102, Method: Compositional matrix adjust. Identities = 191/467 (40%), Positives = 281/467 (60%), Gaps = 11/467 (2%) Query: 5 QSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGLA 64 A W WE +D+ IEG E QQGIRF +FQL+ TY+G+ NIG KG TGE Y G Sbjct: 292 HKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDARLNIGPKGFTGEKYGGAT 351 Query: 65 FWDTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTESC 123 +WDTE+Y +P Y+ P+ +NLL++R LP+A A+E GA YP+ T G E Sbjct: 352 YWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNAREQGLAGALYPMVTFTGIECH 411 Query: 124 DLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSGE 183 + W+ + ++H A+ Y I++Y TGD +L +G E+L+++SRF+A R + +R G+ Sbjct: 412 NEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLEVLVEVSRFWADRVHFSKRRGK 471 Query: 184 YGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRAE 243 Y GV GP+E++ +NNN Y N +A + ++T E LA + PD +RA Sbjct: 472 YMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREALA--KYPRPD--------LNVRAA 521 Query: 244 ELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRYD 303 EL W+ + +M P D + GV+ +HDG+ + + ++ D PL WS+D++ R Sbjct: 522 ELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDLPLNQKWSWDKILRSP 581 Query: 304 MIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAYQ 363 IKQ DVL ++ + F+ + KR N+++YEP +HESSLSP IHSILA E+GKEE+A + Sbjct: 582 FIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPCIHSILAAELGKEEKAVE 641 Query: 364 FFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTSY 423 + RLDLDNYN +T +GLH TS+ +W+ IV GF M++ G +LSF P LP WT Y Sbjct: 642 MYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMKTWGGKLSFAPFLPGAWTGY 701 Query: 424 SFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQIMVDAAGTV 470 +F + Y G L+ + V E V F L+G + VYG+ I ++ + TV Sbjct: 702 AFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDITLNGSHTV 748 >L128690 [G] COG1554 Trehalose and maltose hydrolases (possible phosphorylases) Length = 751 Score = 367 bits (943), Expect = e-101, Method: Compositional matrix adjust. Identities = 187/462 (40%), Positives = 287/462 (62%), Gaps = 13/462 (2%) Query: 2 FADQSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYR 61 + +Q W WE +D++I+G E QQGIRF +FQL+ TY+G+ NIG KG TGE Y Sbjct: 291 YKEQEIAWKKRWEIADVQIDGSAEAQQGIRFNLFQLFSTYYGEDERLNIGPKGFTGEKYG 350 Query: 62 GLAFWDTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGT 120 G +WDTE+Y +P Y+ ++ K AKNLL++R+ LP+A A++ +GA YP+ T G Sbjct: 351 GATYWDTEAYAVPLYLALSDEKVAKNLLKYRHNQLPQAQHNARQQGLKGALYPMVTFTGV 410 Query: 121 ESCDLWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQR 180 E + W+ + ++H A++Y I++Y TGD +L +G E+LI+I+RF+A R + +R Sbjct: 411 ECHNEWEITFEEIHRNGAMAYAIYNYSNYTGDETYLAHEGLEVLIEIARFWADRVHYSKR 470 Query: 181 SGEYGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGL 240 + +Y GV GP+E++ +NNN Y N +A + +T E+LA + P R + Sbjct: 471 NDKYMIHGVTGPNEYENNINNNWYTNKLAAWVLTYTAESLA----KYP-------RPDLI 519 Query: 241 RAEELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLY 300 E+ W+ + M P D + G++ +HDGY + + + + PL NWS+DR+ Sbjct: 520 EGAEIAHWAEIVDKMYYPEDEELGIFVQHDGYLDKDLTPVAQLDPKNLPLNQNWSWDRVL 579 Query: 301 RYDMIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEE 360 R IKQ DVL ++ + +FS K+ N+++YEP +HESSLSPSIH+ILA E+G E++ Sbjct: 580 RSPYIKQADVLQGIYFFGNQFSLAEKQRNFDFYEPLTVHESSLSPSIHAILAAELGMEDK 639 Query: 361 AYQFFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRW 420 A + +E RLDLDNYN +T +GLH TS+ +W+ IV+GF M++ +LSF P LPQ W Sbjct: 640 AVEMYERTARLDLDNYNNDTEDGLHITSMTGSWLAIVHGFAQMKTWDGQLSFAPFLPQAW 699 Query: 421 TSYSFQVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQI 462 T Y+F + Y G LL I+V E V+ + LRG ++ +YG++I Sbjct: 700 TGYAFHINYRGRLLKISVGQE-VKLELLRGQALNLEIYGEKI 740 >lin2226 [G] COG1554 Trehalose and maltose hydrolases (possible phosphorylases) Length = 753 Score = 342 bits (876), Expect = 9e-94, Method: Compositional matrix adjust. Identities = 171/465 (36%), Positives = 266/465 (57%), Gaps = 11/465 (2%) Query: 7 AYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGLAFW 66 A W W+ +D+ I GD QQGIRF IFQL+ TY+G+ NIG KG TGE Y G +W Sbjct: 297 AGWRERWDKADVEIAGDDSAQQGIRFNIFQLFATYYGEDARLNIGPKGFTGEKYGGATYW 356 Query: 67 DTESYCLPFYM-FNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTESCDL 125 DTE++ LP Y+ + + NLL++R+ L A A+++ GA YP+ T G E + Sbjct: 357 DTEAFALPMYLSLTDKSVSHNLLKYRHNQLDGAKINAEKIGLGGALYPMVTFTGVECHNE 416 Query: 126 WQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSGEYG 185 W+ + ++H A++Y I++Y TGD +L + G E+L +I+RF+A R R +Y Sbjct: 417 WEITFEEIHRNGAIAYAIYNYTNYTGDDSYLKTDGIEVLTEITRFWADRVHLSDRLDKYM 476 Query: 186 YFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRAEEL 245 GV GP+E+ V+NN Y N++A +TLE L E ++ G+ E+ Sbjct: 477 IHGVTGPNEYDNNVSNNWYTNYIAAWTIRYTLENLD----------VEAKKRLGVTEYEI 526 Query: 246 TDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRYDMI 305 W M P D ++ +HD + + ++ D P+ NWS+D++ R I Sbjct: 527 AKWEDIEHRMYYPFDEKWQIFVQHDTFLDKELRSTDTLKPEDMPINQNWSWDKILRSCFI 586 Query: 306 KQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAYQFF 365 KQ DVL ++L+ +F + K+ N+E+YEP +HESSLSP++H++LA E+GK ++A + + Sbjct: 587 KQADVLQGLYLFYDDFDFDTKQRNFEFYEPLTVHESSLSPAVHAVLAAELGKYDKAVELY 646 Query: 366 EFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTSYSF 425 + RLDLDN N +T +GLH TS+A +W++IV GF GMR LSF P LP W +Y F Sbjct: 647 KRTARLDLDNINNDTEDGLHITSMAGSWLSIVQGFAGMRVTSGELSFAPFLPNGWDNYRF 706 Query: 426 QVMYEGVLLGITVDSERVRFKALRGGTASILVYGQQIMVDAAGTV 470 ++ + LL + V+ +V K G + +Y ++ +++ A TV Sbjct: 707 KINFRDRLLEVKVEVGQVTVKLCHGEPIHLEMYKKKYLLETAVTV 751 >all4989 [G] COG1554 Trehalose and maltose hydrolases (possible phosphorylases) Length = 759 Score = 259 bits (663), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 143/445 (32%), Positives = 224/445 (50%), Gaps = 18/445 (4%) Query: 1 VFADQSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTY--HGDHPGYNIGAKGLTGE 58 + A A W +W+ SDI IEGD + Q +R+ +FQL H DH +I K L+G Sbjct: 280 LLAAHIAAWEQVWQNSDIIIEGDRQAQLSVRYNLFQLLSVAPRHDDH--VSIAPKTLSGF 337 Query: 59 AYRGLAFWDTESYCLPFYMFNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPI-ATI 117 AY G FWDTE + LPF F P A+NLL +RY TLP A +A+E +GA + + Sbjct: 338 AYSGHIFWDTEIFILPFLTFTQPDLARNLLTYRYHTLPGARRKAQEAGYQGAMFAWESAT 397 Query: 118 DGTESCDLWQHS-----------NLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQ 166 G E W +L++H+ V+Y +WHY + T D ++++ GAE+++ Sbjct: 398 TGDEVTPRWVPGANGELIRIWCGDLEVHITADVAYAVWHYWQTTNDDEWMHDYGAEIILD 457 Query: 167 ISRFYATRGQWGQRSGEYGYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQ 226 + F+ +R QW ++ Y V+GPDE VNNN + N M + + L + Sbjct: 458 TAVFWESRVQWNEQRHSYDILDVIGPDENHDRVNNNAFTNLMVQWHLQSALALWDWFKHV 517 Query: 227 VPDAYAELIRKTGLRAEELTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVT 286 P+ A+L+ + L E L W+ E + + D +G+ E+ +G+F+L +++ Sbjct: 518 YPEKAAQLVEQLNLTTERLHCWAEIQERIFVNEDLTTGLIEQFEGFFQLEDVNLADYEPR 577 Query: 287 DFPLYSNWSYDRLYRYDMIKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHE--SSLS 344 L + + ++KQPDVLM ++L + + N++YY R H SSL Sbjct: 578 TKSLQGLLGIEATSQKQILKQPDVLMLLYLLRQAYDYNTLKVNWDYYTQRTDHTYGSSLG 637 Query: 345 PSIHSILACEIGKEEEAYQFFEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMR 404 P+IH++LAC++ EAY F A +DL++ N EG+H S W +V GFGG+R Sbjct: 638 PAIHAVLACDLNNPTEAYTHFLRAALVDLEDVRLNAAEGIHAASAGGVWQAVVLGFGGVR 697 Query: 405 SDGERLSFRPTLPQRWTSYSFQVMY 429 P LP W F++ + Sbjct: 698 MTQFGPMACPNLPPTWKRLKFKLQW 722 >Rv3401 [G] COG1554 Trehalose and maltose hydrolases (possible phosphorylases) Length = 786 Score = 253 bits (647), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 153/441 (34%), Positives = 226/441 (51%), Gaps = 12/441 (2%) Query: 5 QSAYWSGIWETSDIRIEGDPENQQGIRFCIFQLYQTYHGDHPGYNIGAKGLTGEAYRGLA 64 Q AY W+++D+ +EGDPE QQ +RF +F L Q I +KGLTG Y G A Sbjct: 301 QRAYLDDFWDSADVEVEGDPECQQAVRFGLFHLLQA-SARAERRAIPSKGLTGTGYDGHA 359 Query: 65 FWDTESYCLPFYMFNNPKAAKNLLEFRYKTLPEAVDRAKEVDCEGACYPIATIDGTESCD 124 FWDTE + LP + P A + L +R TL A +RA E+ EGA +P TI G ES Sbjct: 360 FWDTEGFVLPVLTYTAPHAVADALRWRASTLDLAKERAAELGLEGAAFPWRTIRGQESSA 419 Query: 125 LWQHSNLQLHVGTAVSYGIWHYVKNTGDRDFLYSKGAEMLIQISRFYATRGQWGQRSGEY 184 W H+ ++ Y TGD G +LI+ +R + + G R G + Sbjct: 420 YWPAGTAAWHINADIAMAFERYRIVTGDGSLEEECGLAVLIETARLWLSLGH-HDRHGVW 478 Query: 185 GYFGVMGPDEFQLMVNNNCYINFMAKKMFEFTLETLAGMEVQVPDAYAELIRKTGLRAEE 244 GV GPDE+ +V +N + N MA L T A ++ P+A + G+ EE Sbjct: 479 HLDGVTGPDEYTAVVRDNVFTNLMAAH----NLHTAADACLRHPEAAEAM----GVTTEE 530 Query: 245 LTDWSSKAEHMRIPLDPDSGVYEEHDGYFELPHIDIHSIPVTDFPLYSNWSYDRLYRYDM 304 + W A+ IP D + GV+++ +G+ L D + T +PL + +Y RLY + Sbjct: 531 MAAWRDAADAANIPYDEELGVHQQCEGFTTLAEWDFEA--NTTYPLLLHEAYVRLYPAQV 588 Query: 305 IKQPDVLMFMFLYNGEFSREAKRANYEYYEPRCIHESSLSPSIHSILACEIGKEEEAYQF 364 IKQ D+++ M + F+ E K N +YYE R + +SSLS +++ E+G E A+ + Sbjct: 589 IKQADLVLAMQWQSHAFTPEQKARNVDYYERRMVRDSSLSACTQAVMCAEVGHLELAHDY 648 Query: 365 FEFATRLDLDNYNRNTREGLHTTSIAAAWMNIVYGFGGMRSDGERLSFRPTLPQRWTSYS 424 A +DL + +RNTR+GLH S+A AW +V GFGG+R D LS P LP + Sbjct: 649 AYEAALIDLRDLHRNTRDGLHMASLAGAWTALVVGFGGLRDDEGILSIDPQLPDGISRLR 708 Query: 425 FQVMYEGVLLGITVDSERVRF 445 F++ + G L + + V F Sbjct: 709 FRLRWRGFRLIVDANHTDVTF 729 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,285,542 Number of extensions: 1644615 Number of successful extensions: 2047 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 2009 Number of HSP's successfully gapped: 12 Length of query: 479 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 369 Effective length of database: 25,691,691 Effective search space: 9480233979 Effective search space used: 9480233979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)