BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0020.p (272 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YPO0159 [P] COG2116 Formate/nitrite family of transporters 211 9e-55 ZnirCm [P] COG2116 Formate/nitrite family of transporters 202 3e-52 nirCm [P] COG2116 Formate/nitrite family of transporters 202 3e-52 ZnirCm [P] COG2116 Formate/nitrite family of transporters 202 4e-52 STM3476 [P] COG2116 Formate/nitrite family of transporters 201 8e-52 >YPO0159 [P] COG2116 Formate/nitrite family of transporters Length = 268 Score = 211 bits (537), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 121/264 (45%), Positives = 167/264 (63%), Gaps = 12/264 (4%) Query: 1 VFTQSVENIVETAAAKRDKMNESLPKYFLAALLAGAYVGIGIILIFSLGAPLAAVKSPFQ 60 ++T ++ AA ES +++ + +AGAYVG+GIILIF+LG + + Sbjct: 1 MYTDTINKCAANAARIVKLAKESPLGFWIGSAMAGAYVGLGIILIFTLGN---LIDPALR 57 Query: 61 PLIMGTSFGIALTLVVFAGSELFTGNNMFFTVSTLAG---RTSVWDTVKNWIIVFIGNVA 117 PL+MG +FG+ALTLV+ AGSELFTG+ MF T AG + +W + ++GN+ Sbjct: 58 PLVMGATFGLALTLVIIAGSELFTGHTMFLTFGVKAGTIKSSQMWAVLPQ---TWLGNLL 114 Query: 118 GAVVLALLIRGTGLFSAAPAEHLIFTAAAKKMSLPFSELFFRGILCNWLVCLALWMASRA 177 G+V +ALL G + L+ TAA K + P LFF+G+LCNWLVCLA+WMA R Sbjct: 115 GSVFVALLYYYGGGNLLSVDTSLVHTAALAKTTAPAMTLFFKGVLCNWLVCLAIWMAIRV 174 Query: 178 KSETAKLVLIWWCLFAFIASGYEHSVANMTLLSVAVLLPNHPETVSLAGWFHNMIPVTLG 237 + AK + IWWCL AFIASGYEHSVANMTLL+++ +H E +L+G HN++ VTLG Sbjct: 175 EGA-AKFIAIWWCLLAFIASGYEHSVANMTLLALS-WFGHHSEAYTLSGIGHNLLWVTLG 232 Query: 238 NIVGGGVFVGMAYWLISPVRAARP 261 N + G VF+G+ YW +P RA RP Sbjct: 233 NTLSGAVFMGLGYWYATP-RAQRP 255 >ZnirCm [P] COG2116 Formate/nitrite family of transporters Length = 268 Score = 202 bits (515), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 121/265 (45%), Positives = 170/265 (64%), Gaps = 14/265 (5%) Query: 1 VFTQSVENIVETAAAKRDKMNESLP-KYFLAALLAGAYVGIGIILIFSLGAPLAAVKSPF 59 +FT ++ N AA+ +++ + P +++++ +AGAYVG+GIILIF+LG L Sbjct: 1 MFTDTI-NKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL---DPSV 56 Query: 60 QPLIMGTSFGIALTLVVFAGSELFTGNNMFFTVSTLAGRTSVWDTVKNWIIV---FIGNV 116 +PL+MG +FGIALTLV+ AGSELFTG+ MF T AG S + W I+ ++GN+ Sbjct: 57 RPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTIS---HGQMWAILPQTWLGNL 113 Query: 117 AGAVVLALLIRGTGLFSAAPAEHLIFTAAAKKMSLPFSELFFRGILCNWLVCLALWMASR 176 G+V +A+L G ++ + A K + P LFF+G LCNWLVCLA+WMA R Sbjct: 114 VGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALR 173 Query: 177 AKSETAKLVLIWWCLFAFIASGYEHSVANMTLLSVAVLLPNHPETVSLAGWFHNMIPVTL 236 + AK + IWWCL AFIASGYEHS+ANMTL +++ NH E +LAG HN++ VTL Sbjct: 174 TEGA-AKFIAIWWCLLAFIASGYEHSIANMTLFALS-WFGNHSEAYTLAGIGHNLLWVTL 231 Query: 237 GNIVGGGVFVGMAYWLISPVRAARP 261 GN + G VF+G+ YW +P +A RP Sbjct: 232 GNTLSGAVFMGLGYWYATP-KANRP 255 >nirCm [P] COG2116 Formate/nitrite family of transporters Length = 268 Score = 202 bits (515), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 121/265 (45%), Positives = 170/265 (64%), Gaps = 14/265 (5%) Query: 1 VFTQSVENIVETAAAKRDKMNESLP-KYFLAALLAGAYVGIGIILIFSLGAPLAAVKSPF 59 +FT ++ N AA+ +++ + P +++++ +AGAYVG+GIILIF+LG L Sbjct: 1 MFTDTI-NKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL---DPSV 56 Query: 60 QPLIMGTSFGIALTLVVFAGSELFTGNNMFFTVSTLAGRTSVWDTVKNWIIV---FIGNV 116 +PL+MG +FGIALTLV+ AGSELFTG+ MF T AG S + W I+ ++GN+ Sbjct: 57 RPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSIS---HGQMWAILPQTWLGNL 113 Query: 117 AGAVVLALLIRGTGLFSAAPAEHLIFTAAAKKMSLPFSELFFRGILCNWLVCLALWMASR 176 G+V +A+L G ++ + A K + P LFF+G LCNWLVCLA+WMA R Sbjct: 114 VGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALR 173 Query: 177 AKSETAKLVLIWWCLFAFIASGYEHSVANMTLLSVAVLLPNHPETVSLAGWFHNMIPVTL 236 + AK + IWWCL AFIASGYEHS+ANMTL +++ NH E +LAG HN++ VTL Sbjct: 174 TEGA-AKFIAIWWCLLAFIASGYEHSIANMTLFALS-WFGNHSEAYTLAGIGHNLLWVTL 231 Query: 237 GNIVGGGVFVGMAYWLISPVRAARP 261 GN + G VF+G+ YW +P +A RP Sbjct: 232 GNTLSGAVFMGLGYWYATP-KANRP 255 Score = 202 bits (515), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 121/265 (45%), Positives = 170/265 (64%), Gaps = 14/265 (5%) Query: 1 VFTQSVENIVETAAAKRDKMNESLP-KYFLAALLAGAYVGIGIILIFSLGAPLAAVKSPF 59 +FT ++ N AA+ +++ + P +++++ +AGAYVG+GIILIF+LG L Sbjct: 1 MFTDTI-NKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL---DPSV 56 Query: 60 QPLIMGTSFGIALTLVVFAGSELFTGNNMFFTVSTLAGRTSVWDTVKNWIIV---FIGNV 116 +PL+MG +FGIALTLV+ AGSELFTG+ MF T AG S + W I+ ++GN+ Sbjct: 57 RPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTIS---HGQMWAILPQTWLGNL 113 Query: 117 AGAVVLALLIRGTGLFSAAPAEHLIFTAAAKKMSLPFSELFFRGILCNWLVCLALWMASR 176 G+V +A+L G ++ + A K + P LFF+G LCNWLVCLA+WMA R Sbjct: 114 VGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALR 173 Query: 177 AKSETAKLVLIWWCLFAFIASGYEHSVANMTLLSVAVLLPNHPETVSLAGWFHNMIPVTL 236 + AK + IWWCL AFIASGYEHS+ANMTL +++ NH E +LAG HN++ VTL Sbjct: 174 TEGA-AKFIAIWWCLLAFIASGYEHSIANMTLFALS-WFGNHSEAYTLAGIGHNLLWVTL 231 Query: 237 GNIVGGGVFVGMAYWLISPVRAARP 261 GN + G VF+G+ YW +P +A RP Sbjct: 232 GNTLSGAVFMGLGYWYATP-KANRP 255 >STM3476 [P] COG2116 Formate/nitrite family of transporters Length = 269 Score = 201 bits (512), Expect = 8e-52, Method: Compositional matrix adjust. Identities = 121/265 (45%), Positives = 173/265 (65%), Gaps = 14/265 (5%) Query: 1 VFTQSVENIVETAAAKRDKMNESLP-KYFLAALLAGAYVGIGIILIFSLGAPLAAVKSPF 59 +FT ++ N AA+ +++ + P +++++ +AGAYVG+GIILIF+LG L Sbjct: 1 MFTDTI-NKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLL---DPSV 56 Query: 60 QPLIMGTSFGIALTLVVFAGSELFTGNNMFFTVSTLAGRTSVWDTVKNWIIV---FIGNV 116 +PL+MG +FGIALTLV+ AGSELFTG+ MF T+ AG S + W I+ ++GN+ Sbjct: 57 RPLVMGATFGIALTLVIIAGSELFTGHTMFLTLGVKAGTIS---HGQMWAILPQTWLGNL 113 Query: 117 AGAVVLALLIRGTGLFSAAPAEHLIFTAAAKKMSLPFSELFFRGILCNWLVCLALWMASR 176 G+V +ALL G ++ + A K + P + LFF+G LCNWLVCLA+WMA R Sbjct: 114 VGSVFVALLYSWGGGSLLPVDTSIVHSVALAKTTAPATVLFFKGALCNWLVCLAIWMAIR 173 Query: 177 AKSETAKLVLIWWCLFAFIASGYEHSVANMTLLSVAVLLPNHPETVSLAGWFHNMIPVTL 236 + TAK + IWWCL AFIASGYEHSVANMTL +++ +H + +LAG HN++ VTL Sbjct: 174 TEG-TAKFLAIWWCLLAFIASGYEHSVANMTLFALS-WFGHHSDAYTLAGIGHNLLWVTL 231 Query: 237 GNIVGGGVFVGMAYWLISPVRAARP 261 GN + G VF+G+ YW +P ++ RP Sbjct: 232 GNTLSGVVFMGLGYWYATP-KSERP 255 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.326 0.137 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,203,114 Number of extensions: 809856 Number of successful extensions: 2905 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 2845 Number of HSP's successfully gapped: 28 Length of query: 272 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 167 Effective length of database: 26,268,361 Effective search space: 4386816287 Effective search space used: 4386816287 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 115 (48.9 bits)