BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0010.p (311 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1972 [R] COG0673 Predicted dehydrogenases and related proteins 153 4e-37 YPO2042_2 [R] COG0673 Predicted dehydrogenases and related proteins 143 4e-34 STM1169 [R] COG0673 Predicted dehydrogenases and related proteins 137 2e-32 ZmviM [R] COG0673 Predicted dehydrogenases and related proteins 128 1e-29 mviM [R] COG0673 Predicted dehydrogenases and related proteins 128 1e-29 >lin1972 [R] COG0673 Predicted dehydrogenases and related proteins Length = 301 Score = 153 bits (386), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 99/305 (32%), Positives = 154/305 (50%), Gaps = 15/305 (4%) Query: 8 KVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELLSW 67 KVA+VGLG IA+K YLP+ + + +EV + + + ++ + YRF + ++ Sbjct: 3 KVAVVGLGGIAQKAYLPVFAEMENIEV-HLYTRNAQKLKHLSEKYRFDHYHQSIHSMIES 61 Query: 68 GPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVGFN 127 G +A FVHS T +H +++ L+H IPVYVDKP++ +L E + AEEK LL GFN Sbjct: 62 GVNAAFVHSSTASHPEVIRTFLSHNIPVYVDKPIADNLAEVEELTRLAEEKNTLLMTGFN 121 Query: 128 RRFAPMYTAAKAWLDTTGGISACQAFKHRTRLQSGSSRETVXXXXXXXXXXXXWLCGEDA 187 RR+AP Y KA D I+ K+R Q G +R + +L Sbjct: 122 RRYAPKYQELKALTD----INMIVMQKNRAG-QPGEARTFIYDDFIHVIDTVRYLLDAKI 176 Query: 188 ELLHSSLKADDAGRMLQSSGMLGWSQG---ATGMYSMVRDAGADLEKLELHGNGRSAEVA 244 + LH + L SS + S G AT + M RD+G + E+L + EV Sbjct: 177 DQLHVVPVWQNE---LLSSVTVQISAGNKVATAI--MNRDSGVNEERLAVMTKSAKYEVE 231 Query: 245 DMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSIDTPLQCGISASAVLSSHE 304 ++ + +YE G+ R + FG W+T L +RGF + FLN++ + I+ L +H Sbjct: 232 NVTETHIYE-GTTERFERFGDWETTLYKRGFVPIIQAFLNAVRNGEKAPITEEDALETHR 290 Query: 305 LAAKL 309 LA ++ Sbjct: 291 LAEEI 295 >YPO2042_2 [R] COG0673 Predicted dehydrogenases and related proteins Length = 303 Score = 143 bits (360), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 98/310 (31%), Positives = 152/310 (49%), Gaps = 18/310 (5%) Query: 6 RTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYR---FPRGTTDLK 62 + +V IVGLG IA+K YLP+L++ ++VG S + + Q + SYR F R T Sbjct: 3 KLRVGIVGLGGIAQKAYLPILTQAQGWQLVGAFSPNQAKAQPLCDSYRMRYFSRLDT--- 59 Query: 63 ELLSWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLL 122 L+ DAVFVHS T +H+ +V L G+ VYVDKPL+ E+S ++ A+++ L L Sbjct: 60 --LAAASDAVFVHSSTASHFQVVHDLLQAGVHVYVDKPLAETREQSEQLIELADKQHLAL 117 Query: 123 GVGFNRRFAPMYTAAKAWLDTTGGISACQAFKHRTRLQSGSSRE---TVXXXXXXXXXXX 179 VGFNRRFAP+Y K + + + RL S + T+ Sbjct: 118 MVGFNRRFAPLYQQLKQQASSPVSLRM-----EKHRLSSIGPHDLGFTLLDDYLHVVDTA 172 Query: 180 XWLCGEDAELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGR 239 WL GE A L +++ + G+ML + G SM R AG E +++ +G Sbjct: 173 LWLGGEGARLTGGAVQTNAQGQMLYAEHHFQQG-GCLITTSMHRQAGTQRESVQVISDGA 231 Query: 240 SAEVADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSIDTPLQCGISASAV 299 + DM Q G + Q W T LE+RGF+GAV+HF+ ++ + +S Sbjct: 232 CYHITDMRQWQQASAGQVIS-QPAPGWQTTLEQRGFTGAVHHFIEAVSNQTRPQVSGEDA 290 Query: 300 LSSHELAAKL 309 + + + ++ Sbjct: 291 IVAQRMIERI 300 >STM1169 [R] COG0673 Predicted dehydrogenases and related proteins Length = 307 Score = 137 bits (345), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 96/280 (34%), Positives = 138/280 (49%), Gaps = 8/280 (2%) Query: 8 KVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELLSW 67 ++ IVGLG IA+K +LP+L+ + G S S + S+R P D L+ Sbjct: 5 RIGIVGLGGIAQKAWLPVLTNTAGWTLQGAWSPSRDKALRICESWRIP--YVDSLANLAS 62 Query: 68 GPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVGFN 127 DAVFVHS T +HY +V + LN G+ V VDKPL+ +L ++ R+ A +K L L VGFN Sbjct: 63 SCDAVFVHSSTASHYAVVSELLNAGVHVCVDKPLAENLRDAERLVALAAQKKLTLMVGFN 122 Query: 128 RRFAPMYTAAKAWLDTTGGISACQAFKHRT-RLQSGSSRETVXXXXXXXXXXXXWLCGED 186 RRFAP+Y K L T + + KHRT + R T+ WL G + Sbjct: 123 RRFAPLYRELKTRLGTAASL---RMDKHRTDSVGPHDLRFTLLDDYLHVVDTALWLAGGE 179 Query: 187 AELLHSSLKADDAGRMLQSSGMLGWSQGATGMYSMVRDAGADLEKLELHGNGRSAEVADM 246 A L +L ++G M + + SM R AG+ E ++ +G +V DM Sbjct: 180 ARLASGTLLTSESGEMCYAEHHFS-ADKLQITTSMHRRAGSQRESVQAVTDGGLYDVTDM 238 Query: 247 EQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI 286 + E G I+ SW T LE+RGF G HF++ + Sbjct: 239 REWR-EERGQGILIKPIPSWQTTLEQRGFVGCARHFIDCV 277 >ZmviM [R] COG0673 Predicted dehydrogenases and related proteins Length = 307 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 92/284 (32%), Positives = 138/284 (48%), Gaps = 12/284 (4%) Query: 6 RTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELL 65 + ++ +VGLG IA+K +LP+L+ + G S + + + S+R P D L Sbjct: 3 KLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALPICESWRIPYA--DSLSSL 60 Query: 66 SWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVG 125 + DAVFVHS T +H+D+V LN G+ V VDKPL+ +L ++ R+ A K L L VG Sbjct: 61 AASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRDAERLVELAARKKLTLMVG 120 Query: 126 FNRRFAPMYTAAKAWLDTTGGISACQAFKHRTR-LQSGSSRETVXXXXXXXXXXXXWLCG 184 FNRRFAP+Y K L T + + KHR+ + T+ WL G Sbjct: 121 FNRRFAPLYCELKTQLATAASL---RMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSG 177 Query: 185 EDAELLHSSLKADDAGRMLQSSGML--GWSQGATGMYSMVRDAGADLEKLELHGNGRSAE 242 A L +L +DAG ML + G Q T M+ R AG+ E ++ +G + Sbjct: 178 GKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMH---RRAGSQRETVQAVTDGALID 234 Query: 243 VADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI 286 + +M + E G + W + LE+RGF G HF+ + Sbjct: 235 ITEMREWR-EERGQGVVNKPIPGWQSTLEQRGFVGCARHFIECV 277 >mviM [R] COG0673 Predicted dehydrogenases and related proteins Length = 307 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 93/284 (32%), Positives = 138/284 (48%), Gaps = 12/284 (4%) Query: 6 RTKVAIVGLGDIARKVYLPLLSRHDQVEVVGVLSHSPSTVQGVVHSYRFPRGTTDLKELL 65 + ++ +VGLG IA+K +LP+L+ + G S + + + S+R P D L Sbjct: 3 KLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALPICESWRIPYA--DSLSSL 60 Query: 66 SWGPDAVFVHSPTPTHYDIVMKCLNHGIPVYVDKPLSYDLEESRRMAGFAEEKGLLLGVG 125 + DAVFVHS T +H+D+V LN G+ V VDKPL+ +L ++ R+ A K L L VG Sbjct: 61 AASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRDAERLVELAARKKLTLMVG 120 Query: 126 FNRRFAPMYTAAKAWLDTTGGISACQAFKHRTR-LQSGSSRETVXXXXXXXXXXXXWLCG 184 FNRRFAP+Y K L T + + KHR+ + T+ WL G Sbjct: 121 FNRRFAPLYGELKTQLATAASL---RMDKHRSNSVGPHDLYFTLLDDYLHVVDTALWLSG 177 Query: 185 EDAELLHSSLKADDAGRMLQSSGML--GWSQGATGMYSMVRDAGADLEKLELHGNGRSAE 242 A L +L +DAG ML + G Q T M+ R AG+ E ++ +G + Sbjct: 178 GKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMH---RRAGSQRETVQAVTDGALID 234 Query: 243 VADMEQATLYETGSLPRIQHFGSWDTVLERRGFSGAVNHFLNSI 286 + DM + E G + W + LE+RGF G HF+ + Sbjct: 235 ITDMREWR-EERGQGVVHKPIPGWQSTLEQRGFVGCARHFIECV 277 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,415,239 Number of extensions: 916018 Number of successful extensions: 1767 Number of sequences better than 1.0e-05: 95 Number of HSP's gapped: 1740 Number of HSP's successfully gapped: 95 Length of query: 311 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 205 Effective length of database: 26,153,027 Effective search space: 5361370535 Effective search space used: 5361370535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)