BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0008.p (334 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1448 [O] COG0225 Peptide methionine sulfoxide reductase 273 3e-73 SA1257 [O] COG0225 Peptide methionine sulfoxide reductase 218 1e-56 BH2249 [O] COG0225 Peptide methionine sulfoxide reductase 216 3e-56 SPy0466 [O] COG0225 Peptide methionine sulfoxide reductase 211 2e-54 VCA0615_2 [O] COG0229 Conserved domain frequently associated wit... 194 2e-49 >BH1448 [O] COG0225 Peptide methionine sulfoxide reductase Length = 346 Score = 273 bits (698), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 6/167 (3%), Positives = 22/167 (13%) Query: 22 ATFAGGCFWCMVSPFEELPGIVEVVSGYTGGHTVNPTYEEVCSETTGHVEAVQITFQPDI 81 ++ F E + + +V Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETY 68 Query: 82 FPYTKLLELFWQQIDPTDAGGQFYDRGTSYGTAIFTHSEEQRQQAEASKAALQASGRFSA 141 +++ + ++A + Sbjct: 69 VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDL 128 Query: 142 PIVTPILPAKPFYRAEEYHQGYHHKNPGHYKRYRQGSGRDAFIESHW 188 V + Y + + + Sbjct: 129 YQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175 >SA1257 [O] COG0225 Peptide methionine sulfoxide reductase Length = 346 Score = 218 bits (554), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 4/169 (2%), Positives = 23/169 (13%) Query: 20 EKATFAGGCFWCMVSPFEELPGIVEVVSGYTGGHTVNPTYEEVCSETTGHVEAVQITFQP 79 + + + + + + + Q Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 80 DIFPYTKLLELFWQQIDPTDAGGQFYDRGTSYGTAIFTHSEEQRQQAEASKAALQASGRF 139 Y + + + I R+ + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 140 SAPIVTPILPAKPFYRAEEYHQGYHHKNPGHYKRYRQGSGRDAFIESHW 188 + + + + + + Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGK 172 >BH2249 [O] COG0225 Peptide methionine sulfoxide reductase Length = 346 Score = 216 bits (551), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 4/174 (2%), Positives = 24/174 (13%) Query: 15 ISGKTEKATFAGGCFWCMVSPFEELPGIVEVVSGYTGGHTVNPTYEEVCSETTGHVEAVQ 74 + + + + +E + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 75 ITFQPDIFPYTKLLELFWQQIDPTDAGGQFYDRGTSYGTAIFTHSEEQRQQAEASKAALQ 134 + + L + ++ + R G + + + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 135 ASGRFSAPIVTPILPAKPFYRAEEYHQGYHHKNPGHYKRYRQGSGRDAFIESHW 188 + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR 174 >SPy0466 [O] COG0225 Peptide methionine sulfoxide reductase Length = 346 Score = 211 bits (536), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 7/170 (4%), Positives = 27/170 (15%), Gaps = 4/170 (2%) Query: 20 EKATFAGGCFWCMVSPFEELPGIVEVVSGYTGGHTVNPTYEEVCSETTGHVEAVQITFQP 79 + + + + + + V + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 80 DIFPYTKLLELFWQQIDPTDAGGQFYDRGTSYGTAIFTHSEEQRQQAEASKAALQASGRF 139 T + + G S ++ + +A + R Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRL 121 Query: 140 SAPIVTPILPAKPFYRAEEYHQGYHHKNPGHYKRYRQGSG-RDAFIESHW 188 + P + + + + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTD---SAPAVSLLDTLDALAEYQ 168 >VCA0615_2 [O] COG0229 Conserved domain frequently associated with peptide methionine sulfoxide reductase Length = 346 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 10/136 (7%), Positives = 29/136 (21%) Query: 197 LKERLTPLQYEVTQNNATESPFRNEFWDHHGEGIYVDIVSGEPLFSSQDKFDSGCGWPSF 256 L+ L +E+ + +GI + V+ + + Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 257 TRPIRDYSVKEKTDLSHLMIRTEVRSKTADSHLGHVFNDGPGPNGLRYCINSAALRFVPK 316 + S ++ S + + K + + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 317 EDLEKEGYGEYRALFQ 332 + + L Sbjct: 131 VHWPQRPTNCFGKLGY 146 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,972,806 Number of extensions: 1114842 Number of successful extensions: 1566 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 1528 Number of HSP's successfully gapped: 44 Length of query: 334 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 227 Effective length of database: 26,037,693 Effective search space: 5910556311 Effective search space used: 5910556311 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)