BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0350.0003.p (158 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1789 [E] COG0620 Methionine synthase II (cobalamin-independent) 215 2e-56 BS_metC [E] COG0620 Methionine synthase II (cobalamin-independent) 192 2e-49 Cgl1115 [E] COG0620 Methionine synthase II (cobalamin-independent) 182 1e-46 L0100 [E] COG0620 Methionine synthase II (cobalamin-independent) 169 1e-42 ML0100 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ... 169 1e-42 >lin1789 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 765 Score = 215 bits (548), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 103/154 (66%), Positives = 121/154 (78%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK YL+ AV SF++ T V++ TQ+HTHMCYS+F+D+I ISA+ Sbjct: 604 VDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQNDTQIHTHMCYSDFDDIIDTISAL 663 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVISIETSRSHGE+I+ FEE YDK IGLGVYDIHSPRVP V E+ NI RALR +DA Sbjct: 664 DADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVSEIQDNIRRALRAIDA 723 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVR 154 QFWINPDCGLKTR ET AAL++M+ A VR Sbjct: 724 KQFWINPDCGLKTRKEPETIAALQDMIKATKEVR 757 >BS_metC [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 762 Score = 192 bits (488), Expect = 2e-49, Method: Composition-based stats. Identities = 92/154 (59%), Positives = 110/154 (71%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPA+REGLPLK D YL WA +FR+ T+ VK+ TQ+HTHMCYS F D++ I+ + Sbjct: 602 VDEPALREGLPLKTRDWDEYLTWAAEAFRLTTSSVKNETQIHTHMCYSNFEDIVDTINDL 661 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADVI+IE SRSHG + + Y KG+GLGVYDIHSPRVP EEM + I AL V Sbjct: 662 DADVITIEHSRSHGGFLDYLKNHPYLKGLGLGVYDIHSPRVPSTEEMYNIIVDALAVCPT 721 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVR 154 +FW+NPDCGLKTR EET AAL+NMV AA R Sbjct: 722 DRFWVNPDCGLKTRQQEETVAALKNMVEAAKQAR 755 >Cgl1115 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 745 Score = 182 bits (463), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 82/156 (52%), Positives = 112/156 (71%) Query: 1 VDEPAIREGLPLKAEDHAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISAM 60 VDEPAIRE LPL+ D YL W+V SFR+AT D Q+HTHMCYSEFN++I ++ A+ Sbjct: 585 VDEPAIRELLPLRDVDKPAYLQWSVDSFRLATAGAPDDVQIHTHMCYSEFNEVISSVIAL 644 Query: 61 DADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLDA 120 DADV +IE +RS +++AA + ++ G+G GV+DIHSPRVP +E+ +E AL+ +D Sbjct: 645 DADVTTIEAARSDMQVLAALKSSGFELGVGPGVWDIHSPRVPSAQEVDGLLEAALQSVDP 704 Query: 121 GQFWINPDCGLKTRGWEETEAALRNMVLAAANVRKN 156 Q W+NPDCGLKTRGW E EA+L+ +V +A R+ Sbjct: 705 RQLWVNPDCGLKTRGWPEVEASLKVLVESAKQAREK 740 >L0100 [E] COG0620 Methionine synthase II (cobalamin-independent) Length = 759 Score = 169 bits (428), Expect = 1e-42, Method: Composition-based stats. Identities = 81/156 (51%), Positives = 108/156 (69%), Gaps = 1/156 (0%) Query: 1 VDEPAIREGLPLKAED-HAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISA 59 +DE A+RE LPL+ D + YL+WA+ +FR+ + VK TQ+HTHMCYSEF D+I +I A Sbjct: 597 IDEAALREKLPLRKSDWYKEYLDWAIPAFRLVHSKVKPETQIHTHMCYSEFEDIIPSIDA 656 Query: 60 MDADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLD 119 MDADVIS E SRS +I A + + +G GVYDIHSPR+P V+E+ + +E+ L L Sbjct: 657 MDADVISFEASRSELSIIDALKAHHFKTLVGPGVYDIHSPRIPSVKEIQNQLEKILDKLP 716 Query: 120 AGQFWINPDCGLKTRGWEETEAALRNMVLAAANVRK 155 Q W+NPDCGLKTRG ET +LR++V A +RK Sbjct: 717 IEQVWVNPDCGLKTRGNAETIPSLRHLVEATRVLRK 752 >ML0100 [IQ] COG0318 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II Length = 346 Score = 169 bits (428), Expect = 1e-42, Method: Composition-based stats. Identities = 0/156 (0%), Positives = 0/156 (0%), Gaps = 1/156 (0%) Query: 1 VDEPAIREGLPLKAED-HAHYLNWAVRSFRVATNHVKDTTQVHTHMCYSEFNDMIGAISA 59 Sbjct: 597 656 Query: 60 MDADVISIETSRSHGELIAAFEEDDYDKGIGLGVYDIHSPRVPEVEEMTHNIERALRVLD 119 Sbjct: 657 716 Query: 120 AGQFWINPDCGLKTRGWEETEAALRNMVLAAANVRK 155 Sbjct: 717 752 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,905,262 Number of extensions: 475977 Number of successful extensions: 802 Number of sequences better than 1.0e-05: 12 Number of HSP's gapped: 796 Number of HSP's successfully gapped: 12 Length of query: 158 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 60 Effective length of database: 27,075,699 Effective search space: 1624541940 Effective search space used: 1624541940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)