BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0014.p (219 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value aq_436 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 136 3e-32 MA4092 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 81 2e-15 all0082_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 76 3e-14 MT1453_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 59 6e-09 Rv1409_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis 59 6e-09 >aq_436 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 136 bits (342), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 13/191 (6%), Positives = 28/191 (14%), Gaps = 3/191 (1%) Query: 5 IFSQVSIDGKLTLGAGKSSKQLFSMFEEPDIKYIHQFRGLIDAIMVGKNTIQYDDPMLTN 64 S L + D + A M + Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 65 RYEENKNPLRIIPTTHMDISLDSHVL-ADDGRTLIVTTENGKNPEMINILKDRNKDVLIC 123 + + D G + + Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNI-REALHDSLKRLQTDYL 126 Query: 124 GKDKVDFVNLFRELEENYNVHSIMIEGGGFLNWNVLDQDLVDEXXXXXXXXXXXXXTNIT 183 +V + +S + LD + T Sbjct: 127 DLYQVHWPQRPTNCFGK-LGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 184 LVDGTGYTDLN 194 ++ D + Sbjct: 186 VMRYLHLADKH 196 >MA4092 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 13/197 (6%), Positives = 34/197 (17%), Gaps = 16/197 (8%) Query: 5 IFSQVSIDGKLTLGAGKSSKQLFSMFEEPDIKYIHQFRGLIDAIMVGKNTIQYDDPMLTN 64 S L + + + + + + I+ I V + P Sbjct: 8 HSSLEVSTLGLGT---MTFGEQ-NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 65 RYEENK----------NPLRIIPTTHMDISLDSHVLADDGRTLIVTTENGKNPEMINILK 114 E L I + + D + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 115 DRNKDVLICGKDKVDFVNLFRELEENYNVHSIMIEGGGFLNWNVLDQDLVDEXXXXXXXX 174 D + + + + + + L+ Q Sbjct: 124 DYLD-LYQVHWPQRPTNCFGKLGYS-WTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 175 XXXXXTNITLVDGTGYT 191 + Sbjct: 182 TAFGVMRYLHLADKHDL 198 >all0082_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 211 Score = 76.3 bits (186), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 12/163 (7%), Positives = 25/163 (15%), Gaps = 17/163 (10%) Query: 9 VSIDGKLTLGAGKSSKQLFSMFEEPDIK-YIHQFRGLIDAIMVGKNTIQYDDPMLTNRYE 67 + + S E I + + + Sbjct: 13 LRENNPHPTTELNFS-----SPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLEL 67 Query: 68 ENKNPLRIIPTTHMDISLDSHVLADDGRTLIVTTENGKNPEMINILKDRNKDVLICGKDK 127 + I T + S +++ L K Sbjct: 68 GVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPG-------VGRK 120 Query: 128 VDFVNLFRELEENY----NVHSIMIEGGGFLNWNVLDQDLVDE 166 V L + F ++Q Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKL 163 >MT1453_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 58.9 bits (141), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 6/119 (5%), Positives = 19/119 (15%), Gaps = 12/119 (10%) Query: 10 SIDGKLTLGAGKSSKQLFSMFEEPDIKYIHQFRGLIDAIMVGKNTIQYDDPMLTNRYEEN 69 + + + + T + E+ Sbjct: 27 NSEAD-AHAQLDYAVAQGINLIDVAE---MYPVPPRPETQGLTETYVGNWLAKHGSREKL 82 Query: 70 ----KNPLRIIPTTHMDISLDSHVLADDGRTLIVTTENGKNPEMINILKDRNKDVLICG 124 K I D + + R + + + + + Sbjct: 83 IIASKVSGPSR-NNDKGIRPDQALDRKNIREALHDSLKRL--QTDYLDLYQV-HWPQRP 137 >Rv1409_2 [H] COG1985 Pyrimidine reductase, riboflavin biosynthesis Length = 346 Score = 58.9 bits (141), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 6/119 (5%), Positives = 21/119 (17%), Gaps = 12/119 (10%) Query: 10 SIDGKLTLGAGKSSKQLFSMFEEPDIKYIHQFRGLIDAIMVGKNTIQYDDPMLTNRYEEN 69 ++ T+ G+ + + + + + + + Sbjct: 15 TLGLG-TMTFGEQNSEADAHAQLDYA---VAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 70 ----KNPLRIIPTTHMDISLDSHVLADDGRTLIVTTENGKNPEMINILKDRNKDVLICG 124 K+ R D G + + + Sbjct: 71 NWLAKHGSREK-LIIASKVSGPSRNNDKGIRPDQALDRKN--IREALHDSLK-RLQTDY 125 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,806,115 Number of extensions: 594948 Number of successful extensions: 1128 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1129 Number of HSP's successfully gapped: 7 Length of query: 219 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 117 Effective length of database: 26,614,363 Effective search space: 3113880471 Effective search space used: 3113880471 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)