BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0348.0013.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0847 [V] COG0534 Na+-driven multidrug efflux pump 291 1e-78 VC1631 [V] COG0534 Na+-driven multidrug efflux pump 186 7e-47 BB0584 [V] COG0534 Na+-driven multidrug efflux pump 116 8e-26 DR0792 [V] COG0534 Na+-driven multidrug efflux pump 115 1e-25 BB0583 [V] COG0534 Na+-driven multidrug efflux pump 114 2e-25 >CAC0847 [V] COG0534 Na+-driven multidrug efflux pump Length = 459 Score = 291 bits (746), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 152/452 (33%), Positives = 253/452 (55%), Gaps = 2/452 (0%) Query: 1 MLKDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIIT 60 + D K F+ + +AIP+ +Q+ I++ L L D +++G LG+ A+A VG++ + + ++ Sbjct: 6 LFNDKK--FFKAAFGLAIPIIIQNFILSSLNLVDNIIIGGLGETAIAGVGLANQYFFLLD 63 Query: 61 VVLAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFT 120 + L G A+G SI+ AQ+WGK+D K+ + +S IFT+ FP +G F+ Sbjct: 64 LALFGTASGASIFTAQYWGKRDTKNIKRVLGICLITTIILSGIFTIGALAFPNIIIGFFS 123 Query: 121 KDQRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLL 180 +D+ +I+ G +L I SYI ++ +S ILR G VK+PM+ S A+ IN LN+ L Sbjct: 124 QDKNVISLGANYLRIICFSYIVTGISFAYSIILRTVGQVKIPMIVSGTALAINTFLNFSL 183 Query: 181 IFGNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFGAPTALLSKFI 240 ++G F G+ G+A +TLIAR E ++ A YKL+LP + + T + +F Sbjct: 184 VYGIGSFHGIGVYGSAIATLIARFIEFFLMLLAIYKLKLPIAAKLHELLDLSTTFVKRFF 243 Query: 241 RTTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMI 300 + P++L E +W LG T YA +Y+ MGT I A IT + MI Sbjct: 244 KVAVPVMLNESMWGLGVTAYAAVYAHMGTAVIAATNITSTIDRLSMVVFFGFGNAAAIMI 303 Query: 301 GNKLGADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRLVW 360 GN++G ++ A ++KRFI L F+ + +G+++ +P + + ++ + H ++ Sbjct: 304 GNRIGQKDEKTAFLYAKRFIILSPFIGIFMGIILYTSSPAILYAYNINSQVHMYSKEILH 363 Query: 361 VFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFLVY 420 + FLW+K+ N GIL SGGD+KF ++ WL+GVP L +K P+ +VY Sbjct: 364 IIGMFLWIKIFNYTNVVGILRSGGDTKFCLMLDMGGMWLVGVPFVALTGIYFKLPIGIVY 423 Query: 421 MILSLEEVVRLSIGLARIHSRKWMKNLVAEIS 452 + +EE V+L +GL R+ S+KW+ NLV++ S Sbjct: 424 YFVFMEEFVKLLVGLPRLLSKKWINNLVSKPS 455 >VC1631 [V] COG0534 Na+-driven multidrug efflux pump Length = 458 Score = 186 bits (472), Expect = 7e-47, Method: Compositional matrix adjust. Identities = 128/460 (27%), Positives = 223/460 (48%), Gaps = 18/460 (3%) Query: 1 MLKDSKADFYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIIT 60 +L + DF+ ++ IA+P+TLQ+++ + L D LM+GQLG+ +A+VG++ + + T Sbjct: 7 VLSHMRGDFFRRLIAIALPITLQNIMFSSRGLVDVLMLGQLGEAEIAAVGVAARATFVTT 66 Query: 61 VVLAGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFT 120 ++L G+ TG ++ AQ+WG D K + + L FFPQ + L T Sbjct: 67 IMLVGVTTGGALLTAQYWGAGDRKGVRESTALTWLVSNFFAFFTVLLFLFFPQQIMSLTT 126 Query: 121 KDQRLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLL 180 +++ G +L ITS + V + LRA + S + + N+VLN++L Sbjct: 127 DSEQVNALGSQYLIITSFTMFAVACVSSMAVGLRAMHKPGVSTFFSGIGIVSNVVLNWIL 186 Query: 181 IFGNFGFPEWGIKGAATSTLIARLFECCFIIAAAYKLR------LPGVTHIRDWFGAPTA 234 IFG FGFP GI+GAA +T+++ E + + Y + + + DW P Sbjct: 187 IFGKFGFPAMGIQGAAIATVLSGAIEIACLYSYLYGKKHLMAFGWQDIKAVIDW---PK- 242 Query: 235 LLSKFIRTTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXX 294 +S+F+R + P LIW G Y I + G + A+++ P++ Sbjct: 243 -ISRFLRLSLPTTFNFLIWAAGLFTYHAIMGQTGVQGLAALSVMTPVESISLSLLIGMST 301 Query: 295 XXXXMIGNKLGADEQDA----ALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEE 350 ++GN+LGA +A A+ + + +GI V+ IG+ + I P+ + ++ E Sbjct: 302 AAAVLVGNQLGAKNYEAVYYQAIGVTACSVLVGIVVA--IGLYLVQI-PVLGAFTALTPE 358 Query: 351 AHHMGTRLVWVFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSF 410 + + + + + ++ M G+L +GGD KF + A W IG+P + Sbjct: 359 TRELSEKFMLILCLGIVLRSFPMTAIVGVLRAGGDVKFCLGQDIVAQWCIGIPLAAFAAL 418 Query: 411 VWKQPVFLVYMILSLEEVVRLSIGLARIHSRKWMKNLVAE 450 V K +Y++ EEVV+ L R+ S+KWMKNL+ Sbjct: 419 VLKVEPEWIYLLFLTEEVVKWFGSLHRMKSKKWMKNLIEN 458 >BB0584 [V] COG0534 Na+-driven multidrug efflux pump Length = 448 Score = 116 bits (290), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 106/449 (23%), Positives = 195/449 (43%), Gaps = 17/449 (3%) Query: 5 SKAD-FYSKIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVL 63 SK D Y + IAIP ++ + ++LTD MV LGD VA V ++ K + + + Sbjct: 11 SKKDKIYKDLLKIAIPTAIEFFLFNFISLTDNAMVAYLGDYPVAGVSLANKFFELFVTIG 70 Query: 64 AGLATGVSIYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQ 123 +A +I A + + + D KS S F L P F L L + D+ Sbjct: 71 FSMAGAYNIIATRQYNQGDFKSFRNTFFISILTIILFSFPFILISIVNPFFLLRLISNDE 130 Query: 124 RLITEGDIFLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFG 183 R + G +L I S++ ++ + + L+ VK + S+ +V +N V NY+ IF Sbjct: 131 RAVYYGAAYLNIAIFSFVFAIIKGLIANALKVVEIVKFQVYISVFSVLLNFVFNYIFIFV 190 Query: 184 NFGFPEWGIKGAATSTLIARLFEC-CFIIAAAYKLRLPGVTHIR-DWFGAPTALLSKFIR 241 G+ GAA +T + R E +++ A+ + H + D ++ I+ Sbjct: 191 F----HMGVVGAAIATTVIRTLELIVYLVCTAFNKN--SILHFKLDDLNINIKPFAQLIK 244 Query: 242 TTYPIVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIG 301 PI+L E W G V I+ + T + A +I++ + M+G Sbjct: 245 FFIPILLNEFAWFFGYLVLTSIFIGIDTHKYAAYSISFSIYFIIFNIIHSFCISLNIMMG 304 Query: 302 NKLGADEQD---AALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIFQVSEEAHHMGTRL 358 ++ +++ A+ SK +KL S + + + AP IF + +H +G L Sbjct: 305 YEMHNSKKEIMKVAIYLSKIGLKLAFLTSFVLFIF-SFFAPY---IFYTLKYSHLIGIIL 360 Query: 359 VWVFAGFLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVPSGLLMSFVWKQPVFL 418 + ++ +A L G +G F ME + T++ +P +++ P + Sbjct: 361 RYSSVSAFFMALAFQYLF-GFFRAGASPSFGAIMEGSVTFVYTIPIAFVLANYTNLPFEI 419 Query: 419 VYMILSLEEVVRLSIGLARIHSRKWMKNL 447 + I SLE+ ++L + L +S +W+K++ Sbjct: 420 IVFIPSLEDAIKLVVSLPYFYSERWIKSV 448 >DR0792 [V] COG0534 Na+-driven multidrug efflux pump Length = 464 Score = 115 bits (289), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 112/399 (28%), Positives = 182/399 (45%), Gaps = 14/399 (3%) Query: 12 KIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLAGLATGVS 71 +I IA+PV+L+ +I +LT +Q++VG LG VAVA+VG++ + + V L L G S Sbjct: 18 EIAAIAVPVSLEMVIQLVLTFINQVIVGTLGAVAVAAVGLAGSLSFLFFVTLGALGAGTS 77 Query: 72 IYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQRLITEGDI 131 I A+ +G D + T+P L L + Sbjct: 78 ILIARRFGAGDRAGVNHTLSATLLLGTLLGAALTVPTTLGAPSLLRLAGGAPDVTGAAAP 137 Query: 132 FLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGNFGFPEWG 191 +L+I LS +P ++ + S LR+ G + P++ ++++V + +L L+FG G P G Sbjct: 138 YLQIVMLSLVPGLIGGVLSGALRSLGRARTPLVATLLSVALEALLAAGLVFGQLGLPRLG 197 Query: 192 IKGAATSTLIARLFECCFIIAAAYKLR------LPGVTHIRDWFGAPTALLSKFIRTTYP 245 + GAA + A F+ ++ Y R LPG + R+ G AL + P Sbjct: 198 LVGAAWAVAAANTFKALYLAFQVYGPRQLAAFTLPGRSSWREIVGPLLAL-------SAP 250 Query: 246 IVLTELIWVLGETVYAIIYSRMGTVEITAMTITYPLQXXXXXXXXXXXXXXXXMIGNKLG 305 + TE +W LG +Y +++R+GTV + A I L+ ++G LG Sbjct: 251 LAFTEFVWGLGGFMYNAVFARVGTVALAAAGIVTTLEGIFIVGSFGLMSATTVLLGRSLG 310 Query: 306 ADEQDAALDFSKRFIKLGIFVSLGIGVLIAAIAPLYVSIF-QVSEEAHHMGTRLVWVFAG 364 A + A + + G+ L G+L A L +F +V E H+ + + A Sbjct: 311 AGDGAGAQRWLALISRTGLVTGLAFGLLFALSGLLVPLVFPKVGAEVQHIALLGILINAA 370 Query: 365 FLWVKVANMILAGGILNSGGDSKFVFAMEAAATWLIGVP 403 +KV NMIL GG L S GD K V + +L+G+P Sbjct: 371 AQVIKVRNMILGGGALPSVGDGKGVITGDVVGAFLVGLP 409 >BB0583 [V] COG0534 Na+-driven multidrug efflux pump Length = 449 Score = 114 bits (286), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 81/269 (30%), Positives = 139/269 (51%), Gaps = 10/269 (3%) Query: 12 KIYTIAIPVTLQSLIMALLTLTDQLMVGQLGDVAVASVGISIKIYGIITVVLAGLATGVS 71 +++ IAIP +SL+ ++T D M+ LG V V ++ ++ + +++ GL T +S Sbjct: 14 ELFVIAIPTAFESLLFQMVTFFDNFMISYLGSFHVTGVSLANRVTFLFFIIVFGLGTALS 73 Query: 72 IYAAQFWGKKDEKSTAQXXXXXXXXXXXISIIFTLPVHFFPQFCLGLFTKDQRLITEGDI 131 Y +Q KK Q I IIF + FP+ + LFT +Q + G Sbjct: 74 AYVSQAIAKKKFLQVRQSFAYILLIGTTIGIIFFIFSFLFPRNIIKLFTANQDSLNFGSE 133 Query: 132 FLEITSLSYIPVMLTMIFSAILRATGHVKLPMMTSIVAVCINIVLNYLLIFGNFGFPEWG 191 +L+I SLSY+ + + + + ++ VK+P+ + + V INIV NY+LIFG G Sbjct: 134 YLKIISLSYVLMAYSFLSAMGFKSAKEVKIPLYVTSIVVLINIVFNYILIFGF----SMG 189 Query: 192 IKGAATSTLIARLFECCFIIAAAYKLRLPGVTHIRDWFG---APTALLSKFIRTTYPIVL 248 IKGAA +T++AR+ E F+ Y L + ++ R FG AP + ++ P++ Sbjct: 190 IKGAAYATVLARIVE--FVFYFLYSL-ISSNSYYRIKFGDFFAPKVVTRANLKLIIPVLS 246 Query: 249 TELIWVLGETVYAIIYSRMGTVEITAMTI 277 E+ WVL T+ Y+R+G++E + + Sbjct: 247 HEIFWVLSITILHAFYARVGSIEYASFAV 275 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,133,620 Number of extensions: 1110574 Number of successful extensions: 4016 Number of sequences better than 1.0e-05: 89 Number of HSP's gapped: 3895 Number of HSP's successfully gapped: 91 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)