BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0066.p (1142 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 120 1e-26 ECs3958 [G] COG3250 Beta-galactosidase/beta-glucuronidase 120 1e-26 ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 120 1e-26 ebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase 120 1e-26 TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase 113 2e-24 >ZebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 120 bits (301), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 105/382 (27%), Positives = 173/382 (45%), Gaps = 37/382 (9%) Query: 26 ENGEGWYRTVLSLDEVPANQLMSLRFEGVYMNSTLYVNGHVAGEWKYGYSTFEFDITPYL 85 +N G Y+ + +L + + ++F+GV +YVNG G K T EFDI+ + Sbjct: 119 DNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMV 178 Query: 86 TVGDNMIYMRVIHEAPNS------RWYSGAGIYRPVWLKTTQKTHIAPDGIYISARPAGG 139 GDN++ +RV+ A ++ W+S AGI+R V+L THI + + Sbjct: 179 KTGDNLLCVRVMQWADSTYVEDQDMWWS-AGIFRDVYLVGKHLTHI--NDFTVRTDFDEA 235 Query: 140 GCWTVDVDCELRVEE-GREPSGLKLRHTILDARGTVIRQEESVVHAEA-GHQPVHTHSR- 196 C + CE+ +E P L +T+ D E VVH+ A H + + Sbjct: 236 YC-DATLSCEVVLENLAASPVVTTLEYTLFDG--------ERVVHSSAIDHLAIEKLTSA 286 Query: 197 ---LTVERPQLWDIASPHLYTLRTELWXXX-XXXXXXXXXFGFRTVALDSQKGFFLNGRH 252 TVE+PQ W SP+LY L L GFR + + F++N R+ Sbjct: 287 SFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV-RDGLFWINNRY 345 Query: 253 VKLYGVCQHHDLGCLGAAVNKAALRRQFVLLQEMGVNAIRTAHNMPAVELMELADEMGVL 312 V L+GV +H + G AV + + L+++ +N++RTAH EL D G+ Sbjct: 346 VMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLF 405 Query: 313 IVSEAFDMWERSKTAYDYARFF--PEW---WTKDVASWVRRDRNHPSLIMWSIGNE---- 363 +++E D +R P+W + + + + +NHPS+I+WS+GNE Sbjct: 406 VMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYG 465 Query: 364 --IYDTHAGARGQELTRMLRDE 383 I + A+ + TR++ E Sbjct: 466 CNIRAMYHAAKALDDTRLVHYE 487 >ECs3958 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 120 bits (301), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 105/382 (27%), Positives = 173/382 (45%), Gaps = 37/382 (9%) Query: 26 ENGEGWYRTVLSLDEVPANQLMSLRFEGVYMNSTLYVNGHVAGEWKYGYSTFEFDITPYL 85 +N G Y+ + +L + + ++F+GV +YVNG G K T EFDI+ + Sbjct: 119 DNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMV 178 Query: 86 TVGDNMIYMRVIHEAPNS------RWYSGAGIYRPVWLKTTQKTHIAPDGIYISARPAGG 139 GDN++ +RV+ A ++ W+S AGI+R V+L THI + + Sbjct: 179 KTGDNLLCVRVMQWADSTYVEDQDMWWS-AGIFRDVYLVGKHLTHI--NDFTVRTDFDEA 235 Query: 140 GCWTVDVDCELRVEE-GREPSGLKLRHTILDARGTVIRQEESVVHAEA-GHQPVHTHSR- 196 C + CE+ +E P L +T+ D E VVH+ A H + + Sbjct: 236 YC-DATLSCEVVLENLAASPVVTTLEYTLFDG--------ERVVHSSAIDHLAIEKLTSA 286 Query: 197 ---LTVERPQLWDIASPHLYTLRTELWXXX-XXXXXXXXXFGFRTVALDSQKGFFLNGRH 252 TVE+PQ W SP+LY L L GFR + + F++N R+ Sbjct: 287 SFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV-RDGLFWINNRY 345 Query: 253 VKLYGVCQHHDLGCLGAAVNKAALRRQFVLLQEMGVNAIRTAHNMPAVELMELADEMGVL 312 V L+GV +H + G AV + + L+++ +N++RTAH EL D G+ Sbjct: 346 VMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLF 405 Query: 313 IVSEAFDMWERSKTAYDYARFF--PEW---WTKDVASWVRRDRNHPSLIMWSIGNE---- 363 +++E D +R P+W + + + + +NHPS+I+WS+GNE Sbjct: 406 VMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYG 465 Query: 364 --IYDTHAGARGQELTRMLRDE 383 I + A+ + TR++ E Sbjct: 466 CNIRAMYHAAKALDDTRLVHYE 487 Score = 120 bits (301), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 105/382 (27%), Positives = 173/382 (45%), Gaps = 37/382 (9%) Query: 26 ENGEGWYRTVLSLDEVPANQLMSLRFEGVYMNSTLYVNGHVAGEWKYGYSTFEFDITPYL 85 +N G Y+ + +L + + ++F+GV +YVNG G K T EFDI+ + Sbjct: 119 DNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMV 178 Query: 86 TVGDNMIYMRVIHEAPNS------RWYSGAGIYRPVWLKTTQKTHIAPDGIYISARPAGG 139 GDN++ +RV+ A ++ W+S AGI+R V+L THI + + Sbjct: 179 KTGDNLLCVRVMQWADSTYVEDQDMWWS-AGIFRDVYLVGKHLTHI--NDFTVRTDFDEA 235 Query: 140 GCWTVDVDCELRVEE-GREPSGLKLRHTILDARGTVIRQEESVVHAEA-GHQPVHTHSR- 196 C + CE+ +E P L +T+ D E VVH+ A H + + Sbjct: 236 YC-DATLSCEVVLENLAASPVVTTLEYTLFDG--------ERVVHSSAIDHLAIEKLTSA 286 Query: 197 ---LTVERPQLWDIASPHLYTLRTELWXXX-XXXXXXXXXFGFRTVALDSQKGFFLNGRH 252 TVE+PQ W SP+LY L L GFR + + F++N R+ Sbjct: 287 SFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV-RDGLFWINNRY 345 Query: 253 VKLYGVCQHHDLGCLGAAVNKAALRRQFVLLQEMGVNAIRTAHNMPAVELMELADEMGVL 312 V L+GV +H + G AV + + L+++ +N++RTAH EL D G+ Sbjct: 346 VMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLF 405 Query: 313 IVSEAFDMWERSKTAYDYARFF--PEW---WTKDVASWVRRDRNHPSLIMWSIGNE---- 363 +++E D +R P+W + + + + +NHPS+I+WS+GNE Sbjct: 406 VMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYG 465 Query: 364 --IYDTHAGARGQELTRMLRDE 383 I + A+ + TR++ E Sbjct: 466 CNIRAMYHAAKALDDTRLVHYE 487 >ebgA [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 1042 Score = 120 bits (301), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 105/382 (27%), Positives = 173/382 (45%), Gaps = 37/382 (9%) Query: 26 ENGEGWYRTVLSLDEVPANQLMSLRFEGVYMNSTLYVNGHVAGEWKYGYSTFEFDITPYL 85 +N G Y+ + +L + + ++F+GV +YVNG G K T EFDI+ + Sbjct: 119 DNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMV 178 Query: 86 TVGDNMIYMRVIHEAPNS------RWYSGAGIYRPVWLKTTQKTHIAPDGIYISARPAGG 139 GDN++ +RV+ A ++ W+S AGI+R V+L THI + + Sbjct: 179 KTGDNLLCVRVMQWADSTYVEDQDMWWS-AGIFRDVYLVGKHLTHI--NDFTVRTDFDEA 235 Query: 140 GCWTVDVDCELRVEE-GREPSGLKLRHTILDARGTVIRQEESVVHAEA-GHQPVHTHSR- 196 C + CE+ +E P L +T+ D E VVH+ A H + + Sbjct: 236 YC-DATLSCEVVLENLAASPVVTTLEYTLFDG--------ERVVHSSAIDHLAIEKLTSA 286 Query: 197 ---LTVERPQLWDIASPHLYTLRTELWXXX-XXXXXXXXXFGFRTVALDSQKGFFLNGRH 252 TVE+PQ W SP+LY L L GFR + + F++N R+ Sbjct: 287 TFAFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKV-RDGLFWINNRY 345 Query: 253 VKLYGVCQHHDLGCLGAAVNKAALRRQFVLLQEMGVNAIRTAHNMPAVELMELADEMGVL 312 V L+GV +H + G AV + + L+++ +N++RTAH EL D G+ Sbjct: 346 VMLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGLF 405 Query: 313 IVSEAFDMWERSKTAYDYARFF--PEW---WTKDVASWVRRDRNHPSLIMWSIGNE---- 363 +++E D +R P+W + + + + +NHPS+I+WS+GNE Sbjct: 406 VMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNESGYG 465 Query: 364 --IYDTHAGARGQELTRMLRDE 383 I + A+ + TR++ E Sbjct: 466 CNIRAMYHAAKALDDTRLVHYE 487 >TM1062 [G] COG3250 Beta-galactosidase/beta-glucuronidase Length = 563 Score = 113 bits (282), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 98/392 (25%), Positives = 157/392 (40%), Gaps = 54/392 (13%) Query: 9 PVSLPHDWLIYNTHALYENGEGWYRTVLSLDEVPANQLMSLRFEGVYMNSTLYVNGHVAG 68 P+++P W YE G Y+T + + + + + L F V + +++NG G Sbjct: 29 PIAVPGSWNEQYQDLCYEEGPFTYKTTFYVPKELSQKHIRLYFAAVNTDCEVFLNGEKVG 88 Query: 69 EWKYGYSTFEFDITPYLTVGDNMIYMRVIHE---------------------------AP 101 E Y FE D+T + G+N +RV+ E Sbjct: 89 ENHIEYLPFEVDVTGKVKSGENE--LRVVVENRLKVGGFPSKVPDSGTHTVGFFGSFPPA 146 Query: 102 NSRWYSGAGIYRPVWLKTTQKTHIAPDGIYISARPAGGGCWTVDVDCELRVEEGREPSGL 161 N ++ GI RPV ++ T I + S V V E+ E + + Sbjct: 147 NFDFFPYGGIIRPVLIEFTDHARILDIWVDTSESEPEKKLGKVKVKIEVSEEAVGQEMTI 206 Query: 162 KLRHTILDARGTVIRQEESVVHAEAGHQPVHTHSRLTVERPQLWDIASPHLYTLRTELWX 221 KL IR V E +E + W + P+LY L+ EL Sbjct: 207 KL-----GEEEKKIRTSNRFVEGE-----------FILENARFWSLEDPYLYPLKVEL-- 248 Query: 222 XXXXXXXXXXXFGFRTVALDSQKGFFLNGRHVKLYGVCQHHDLGCLGAAVNKAALRRQFV 281 G RT++ D +K +LNG+ V L G +H + LG + + F Sbjct: 249 ---EKDEYTLDIGIRTISWD-EKRLYLNGKPVFLKGFGKHEEFPVLGQGTFYPLMIKDFN 304 Query: 282 LLQEMGVNAIRTAHNMPAVELMELADEMGVLIVSEAFDMWERSKTAYDYARFFPEWWTKD 341 LL+ + N+ RT+H + E ++LAD +G+L++ EA + T Y Y + + Sbjct: 305 LLKWINANSFRTSHYPYSEEWLDLADRLGILVIDEAPHV---GITRYHYNPETQKIAEDN 361 Query: 342 VASWVRRDRNHPSLIMWSIGNEIYDTHAGARG 373 + + R +NHPS+IMWS+ NE H A G Sbjct: 362 IRRMIDRHKNHPSVIMWSVANEPESNHPDAEG 393 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 89,744,235 Number of extensions: 4021644 Number of successful extensions: 5608 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 5564 Number of HSP's successfully gapped: 16 Length of query: 1142 Length of database: 38,378,431 Length adjustment: 117 Effective length of query: 1025 Effective length of database: 24,884,353 Effective search space: 25506461825 Effective search space used: 25506461825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 121 (51.2 bits)