BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0054.p (452 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH0905 [G] COG1653 ABC-type sugar transport system, periplasmic ... 403 e-112 BS_araN [G] COG1653 ABC-type sugar transport system, periplasmic... 390 e-108 SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmi... 81 4e-15 AGpA775 [E] COG0410 ABC-type branched-chain amino acid transport... 75 2e-13 AGpA778 [E] COG4177 ABC-type branched-chain amino acid transport... 75 2e-13 >BH0905 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 445 Score = 403 bits (1036), Expect = e-112, Method: Compositional matrix adjust. Identities = 198/445 (44%), Positives = 286/445 (64%), Gaps = 23/445 (5%) Query: 14 ILVMGLSGCGGNGNTETSPSDQPAQNNSGSKATEGSETAKPPAEAEKVVFWTFVDAHQKF 73 ++V+ L CGG+ ++ + + + ATE ++FWTF H Sbjct: 13 VMVLVLVACGGSSSSSSDADETSVIGDDIEGATE-------------LIFWTFAGQHVDL 59 Query: 74 YESMAASWNESHPDKAIALEATTIPYDDMHTKLLVALQSGVGAPDLVDIEQSKFPNFMKG 133 +E SWNE PD+ I L A T P+D MH LL+ALQSG GAPDL DIE S+FPNF++G Sbjct: 60 FEDAVVSWNEEFPDRPIKLVAETYPFDQMHNNLLLALQSGSGAPDLADIEVSRFPNFLQG 119 Query: 134 TPQLVSLNDIVEPELGNIVKSRVEIYSKDNTYYGIDYHVGATVMYYNKEMLDQAGVNPDT 193 PQL+ +ND VEP + V++R +Y+KD YYGI HVGA+VMYYNKE++D+AGV+ ++ Sbjct: 120 VPQLLPMNDHVEPVIDKFVEARFNLYAKDGEYYGIPTHVGASVMYYNKEIMDEAGVDIES 179 Query: 194 IKTWADFETAGKQVLDKTGKPMITFEGNGNWSWWPAISQQGSDQVDENGNVTVDTPVNVK 253 I+TW D+ AGKQV+++TGK M T + + +SQ+GSD DENGN+T+DT N++ Sbjct: 180 IETWDDYVEAGKQVVERTGKVMTTVPTDDYLPMFQMVSQRGSDFFDENGNLTLDTQENIE 239 Query: 254 TMEFFQKMVK-EGVAAVAPGAGHDTEEYFGYMDSGSAASVFMPFWFMNRFTDHMPDLKGN 312 ++F ++ +A + PG EEY+ YM+ G+ AS+ MP W+M RF D+MPDL G Sbjct: 240 VLQFLYDLIYVHEIAELTPGGQPHAEEYYQYMNDGNVASMAMPIWYMGRFLDNMPDLAGK 299 Query: 313 IIIRPMPAWEEGGNRSAGMGGTATSITNQAKNPGLIKEFLAYAKLSKEGNLQVWKQLGFD 372 ++I+P+PAWEEGG RSAGMGGT T +TNQ + L K+FLAYAK+S++ N ++W LGFD Sbjct: 300 MLIQPLPAWEEGGFRSAGMGGTGTVVTNQTDHEELAKDFLAYAKISEKANEKLWTILGFD 359 Query: 373 PIRTDVWSDPAMKESNKFTDYFGTDIFDTLLSVKDEIAPVHIREKSPEVFDAIRNKAMPQ 432 P R DVW +P +E N F +FG +IF+ LL V+DEI ++I + +P ++ N+ Sbjct: 360 PPRWDVWDNPVFQEDNDFYQFFGENIFEVLLDVRDEINSINISQYTP----SVANEFSTN 415 Query: 433 IFIDM-----KDPAQVLKEVQASLK 452 IF D+ P + LK+ Q +++ Sbjct: 416 IFNDVLRQQTHTPEEALKKAQETIE 440 >BS_araN [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 433 Score = 390 bits (1002), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/397 (46%), Positives = 259/397 (65%), Gaps = 4/397 (1%) Query: 60 KVVFWTFVDAHQKFYESMAASWNESHPDKAIALEATTIPYDDMHTKLLVALQSGVGAPDL 119 ++ FWTF H++FY M WN+ +PD+ I L PY MH L ++L +G G PD+ Sbjct: 35 ELTFWTFSGLHEQFYVEMVKEWNKKYPDRKIKLNTVVYPYGQMHDNLSISLIAGEGVPDI 94 Query: 120 VDIEQSKFPNFMKGTP-QLVSLNDIVEPELGNIVKSRVEIYSKDNTYYGIDYHVGATVMY 178 D+E ++F NF+KG+ L L ++E + V++R+ +YSK+ YG+D HVG TVM+ Sbjct: 95 ADVELARFSNFLKGSDIPLADLTPLIEKDRDKFVEARLTLYSKNGKLYGLDTHVGTTVMF 154 Query: 179 YNKEMLDQAGVNPDTIKTWADFETAGKQVLDKTGKPMITFEGNGNWSWWPAISQQGSDQV 238 YN +++ +AGVNPD IKTW D+ AG++V TGKPM T E N + ++ ISQQ S Sbjct: 155 YNMDVMKKAGVNPDDIKTWDDYHKAGQKVRKVTGKPMGTVETNDSATFLSMISQQNSGYF 214 Query: 239 DENGNVTVDTPVNVKTMEFFQKMVKEGVAAVAPGAGHDTEEYFGYMDSGSAASVFMPFWF 298 D+NG + ++ NVKT+++ + M+ + APG GH +EEY+G+M+ G AASV MP W+ Sbjct: 215 DKNGKLILNNDTNVKTLQYLKDMINDKTMIPAPGGGHHSEEYYGFMNQGGAASVLMPIWY 274 Query: 299 MNRFTDHMPDLKGNIIIRPMPAWEEGGNRSAGMGGTATSITNQAKNPGLIKEFLAYAKLS 358 M RF D+MPDLKG I IRP+PAW+EGG+RSAG+GGTAT + Q+K+ L KEFLA+AK S Sbjct: 275 MGRFIDYMPDLKGKIAIRPLPAWKEGGDRSAGLGGTATVVPKQSKHVELAKEFLAFAKGS 334 Query: 359 KEGNLQVWKQLGFDPIRTDVWSDPAMKESNKFTDYF--GTDIFDTLLSVKDEIAPVHIRE 416 +EGN ++W LGFDP+R DVWS +KE NK+TDYF GT IF LL +KDEI P+++ E Sbjct: 335 EEGNKKLWSVLGFDPLRWDVWSSKELKEKNKYTDYFQNGTGIFSVLLDIKDEINPIYLHE 394 Query: 417 KSPEVFDAI-RNKAMPQIFIDMKDPAQVLKEVQASLK 452 + D + R+ + K P Q L LK Sbjct: 395 DFAKASDLVNRSVLFDALKSQQKTPKQALDRAAGELK 431 >SMb20231 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 451 Score = 80.9 bits (198), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 86/320 (26%), Positives = 134/320 (41%), Gaps = 30/320 (9%) Query: 56 AEAEKVVFWTF------VDAHQKFYESMAASWNESHPDKAIALEATTIPYDDMHT--KLL 107 A+ + FW F V + ++E A WN+SHP K +E IP + + KL Sbjct: 43 AQETPLKFWQFYAPGGPVASQVAWFEKTVADWNDSHPQK---IELEFIPNSEYISGPKLA 99 Query: 108 VALQSGVGAPDLVDIEQSKFPNFMKGTPQLVSLNDIVEPEL-GNIVKSRVEIYSKDNTYY 166 A SG G PD+ I F + G L L ++ + + +S + D + Sbjct: 100 TAFASGEG-PDIFIISPGDFLRYYNGG-VLKDLTPFIDDKAKADFPESVIANRMVDGKIF 157 Query: 167 GIDYHVGATVMYYNKEMLDQAGVNP-DTIKTW-ADFETAGKQVLDKTGKPMITFEGN--- 221 GI V MYY+ + ++AG+N D KTW A E A K L + + FE Sbjct: 158 GIPMEVEPMAMYYSVKAFEEAGLNENDVPKTWEALLEVAKK--LTTPERFGVMFETQPGY 215 Query: 222 -GNWSWWPAISQQGSDQVDENGNVTVDTPVNVKTMEFFQKMVKEGVA--AVAPGAGHDTE 278 N++W+P + Q G + +G + D+P V+ ++F+Q + GVA + GHDT Sbjct: 216 YQNFTWYPFLWQGGGEFQTADGKSSFDSPATVQALKFWQDAINSGVAPRQIMGAGGHDT- 274 Query: 279 EYFGYMDSGSAASVFMPFWFMNRFTDHMPDLKGNIIIRPMPAWEEGGNRSAGMGGTATSI 338 + SG A + W +++ + D + + P P G GG A Sbjct: 275 --IANLASGYVAMQNVGIWGISQLQSNAKDFQYGVFRLPTP---PNGKYVTVGGGWAFVA 329 Query: 339 TNQAKNPGLIKEFLAYAKLS 358 N EF A+A S Sbjct: 330 NANGHNSDAAGEFCAWALAS 349 >AGpA775 [E] COG0410 ABC-type branched-chain amino acid transport systems, ATPase component Length = 373 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 17/342 (4%), Positives = 49/342 (14%), Gaps = 27/342 (7%) Query: 58 AEKVVFW-TFVDAHQKFYESMAASWNESHPDKAIALEATTI-PYDDMHTKLLVALQSGVG 115 + + + I A + Sbjct: 26 SLRKCSERREEKKMTRTIRWGVLGCAGIAAKAVI--PAIQSSRLGRVVAIASRDAAKA-K 82 Query: 116 APDLVDIEQSKFPNFMKGTPQLVSLNDI-----VEPELGNIVKSRVEIYSKDNTYYGIDY 170 + ++ + +I P ++ + + Sbjct: 83 EMTARFGIARSYGSYEDLL----ADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPI 138 Query: 171 HVGATVMYYNKEMLDQAGVNPDT-IKTWADFETAGKQVLDKTGKPMIT-FEGNGNWSWWP 228 + A A + + L G+ + Sbjct: 139 ALDAAQARELATAQKAASLPVAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLD 198 Query: 229 AISQQGSDQVD-ENGNVTVDTPVNVKTMEFFQKMVKEGVAAVAPGAGHDTEEYFGYMDSG 287 + G V F +A + + SG Sbjct: 199 DPGNVRNQVDIGGGGLFDVGCYAINTARFLFDAEPLRAIALMERDP---VFGTDR-LTSG 254 Query: 288 SAASVFMPFWFMNRFTDHMPDLKGNIIIRPMPAWEEGGNRSAGMGGTATSI--TNQAKNP 345 A T K ++ ++ + ++ Sbjct: 255 LLAFPEGKQLAFTCSTQLSLTQKVTVLGTRGRL---EIPIPFNAPADEPTVLIFDDGRDL 311 Query: 346 GLIKEFLAYAKLSKEGNLQVWKQLGFDPIRTDVWSDPAMKES 387 + + + Sbjct: 312 AGGGREEIMIG-PVDQYREQADAFAEAVLSGKPLETDLGDAI 352 >AGpA778 [E] COG4177 ABC-type branched-chain amino acid transport system, permease component Length = 373 Score = 75.5 bits (184), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 17/342 (4%), Positives = 49/342 (14%), Gaps = 27/342 (7%) Query: 58 AEKVVFW-TFVDAHQKFYESMAASWNESHPDKAIALEATTI-PYDDMHTKLLVALQSGVG 115 + + + I A + Sbjct: 26 SLRKCSERREEKKMTRTIRWGVLGCAGIAAKAVI--PAIQSSRLGRVVAIASRDAAKA-K 82 Query: 116 APDLVDIEQSKFPNFMKGTPQLVSLNDI-----VEPELGNIVKSRVEIYSKDNTYYGIDY 170 + ++ + +I P ++ + + Sbjct: 83 EMTARFGIARSYGSYEDLL----ADPEIDAIYNPLPNHLHVPMTIKALEQGKPVLCEKPI 138 Query: 171 HVGATVMYYNKEMLDQAGVNPDT-IKTWADFETAGKQVLDKTGKPMIT-FEGNGNWSWWP 228 + A A + + L G+ + Sbjct: 139 ALDAAQARELATAQKAASLPVAEAFMVRHHPQWKKAHALITEGRLGEVRAIQTIFSYYLD 198 Query: 229 AISQQGSDQVD-ENGNVTVDTPVNVKTMEFFQKMVKEGVAAVAPGAGHDTEEYFGYMDSG 287 + G V F +A + + SG Sbjct: 199 DPGNVRNQVDIGGGGLFDVGCYAINTARFLFDAEPLRAIALMERDP---VFGTDR-LTSG 254 Query: 288 SAASVFMPFWFMNRFTDHMPDLKGNIIIRPMPAWEEGGNRSAGMGGTATSI--TNQAKNP 345 A T K ++ ++ + ++ Sbjct: 255 LLAFPEGKQLAFTCSTQLSLTQKVTVLGTRGRL---EIPIPFNAPADEPTVLIFDDGRDL 311 Query: 346 GLIKEFLAYAKLSKEGNLQVWKQLGFDPIRTDVWSDPAMKES 387 + + + Sbjct: 312 AGGGREEIMIG-PVDQYREQADAFAEAVLSGKPLETDLGDAI 352 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.132 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,837,595 Number of extensions: 1648267 Number of successful extensions: 2794 Number of sequences better than 1.0e-05: 30 Number of HSP's gapped: 2791 Number of HSP's successfully gapped: 30 Length of query: 452 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 342 Effective length of database: 25,691,691 Effective search space: 8786558322 Effective search space used: 8786558322 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)