BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0048.p (449 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Rv2041c [G] COG1653 ABC-type sugar transport system, periplasmic... 90 9e-18 MT2101 [G] COG1653 ABC-type sugar transport system, periplasmic ... 90 9e-18 ML1427 [G] COG1653 ABC-type sugar transport system, periplasmic ... 89 2e-17 ML1770 [G] COG1653 ABC-type sugar transport system, periplasmic ... 87 6e-17 Rv2318 [G] COG1653 ABC-type sugar transport system, periplasmic ... 84 4e-16 >Rv2041c [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 439 Score = 89.7 bits (221), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 99/349 (28%), Positives = 153/349 (43%), Gaps = 46/349 (13%) Query: 60 LKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGNEAPDL--- 116 L F F +P E + F +YP+IKV A+ + ++ AG + PD+ Sbjct: 38 LTFFFAANPDELRPRMRVVNEFQRRYPDIKVRAL-LSGPGVMQQLATFCAGGKCPDVLMA 96 Query: 117 -GYMHGELADPWQKDGKFVNMFELLDADK----ELKRSDFLDYIWFKSSEDFAY--GIST 169 + ELAD G +++ LL D+ ELK SD + ++ E F + G Sbjct: 97 WELTYAELAD----RGVLLDLNTLLARDQAFAAELK-SDSIGALY----ETFTFNGGQYA 147 Query: 170 AGETFG---LFYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLTLDKNGKNAADPGFD 226 E + LFYNK LFD A V PP E WS+ EF+ AQ LT K G++ D Sbjct: 148 FPEQWSGNFLFYNKQLFDDAGVPPPPGSWERPWSFAEFLDAAQALT--KQGRSGRD---- 201 Query: 227 PDNIKQFGVSFETWHGPVHATV--INNEGEWVT---KDGKFGFSQPEAAEAIQRLADLIN 281 +Q+G W A + +NN W F EA+Q ADL N Sbjct: 202 ----RQWGF-VNAWVSFYAAGLFAMNNGVPWSVPRMNPTHLNFDHDGFLEAVQFYADLTN 256 Query: 282 VYHVAPSPVQMKSIPSQAVALQSKLAAMSMHGQWINLDLGAAK-VNYDIGVLPKMKR--- 337 + VAPS + +S+ S A A +++ G W A +++D+ LP R Sbjct: 257 KHKVAPSAAEQQSM-STADLFSVGKAGIALAGHWRYQTFDRADGLDFDVAPLPIGPRGRA 315 Query: 338 -SVPMGISGASVIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMPTM 385 +G++G + I +S+ +++AW KF P G + + L++P + Sbjct: 316 ACSDIGVTGLA-IAATSRRKDQAWEFVKFATGPVGQALIGESRLFVPVL 363 >MT2101 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 439 Score = 89.7 bits (221), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 99/349 (28%), Positives = 153/349 (43%), Gaps = 46/349 (13%) Query: 60 LKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGNEAPDL--- 116 L F F +P E + F +YP+IKV A+ + ++ AG + PD+ Sbjct: 38 LTFFFAANPDELRPRMRVVNEFQRRYPDIKVRAL-LSGPGVMQQLATFCAGGKCPDVLMA 96 Query: 117 -GYMHGELADPWQKDGKFVNMFELLDADK----ELKRSDFLDYIWFKSSEDFAY--GIST 169 + ELAD G +++ LL D+ ELK SD + ++ E F + G Sbjct: 97 WELTYAELAD----RGVLLDLNTLLARDQAFAAELK-SDSIGALY----ETFTFNGGQYA 147 Query: 170 AGETFG---LFYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLTLDKNGKNAADPGFD 226 E + LFYNK LFD A V PP E WS+ EF+ AQ LT K G++ D Sbjct: 148 FPEQWSGNFLFYNKQLFDDAGVPPPPGSWERPWSFAEFLDAAQALT--KQGRSGRD---- 201 Query: 227 PDNIKQFGVSFETWHGPVHATV--INNEGEWVT---KDGKFGFSQPEAAEAIQRLADLIN 281 +Q+G W A + +NN W F EA+Q ADL N Sbjct: 202 ----RQWGF-VNAWVSFYAAGLFAMNNGVPWSVPRMNPTHLNFDHDGFLEAVQFYADLTN 256 Query: 282 VYHVAPSPVQMKSIPSQAVALQSKLAAMSMHGQWINLDLGAAK-VNYDIGVLPKMKR--- 337 + VAPS + +S+ S A A +++ G W A +++D+ LP R Sbjct: 257 KHKVAPSAAEQQSM-STADLFSVGKAGIALAGHWRYQTFDRADGLDFDVAPLPIGPRGRA 315 Query: 338 -SVPMGISGASVIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMPTM 385 +G++G + I +S+ +++AW KF P G + + L++P + Sbjct: 316 ACSDIGVTGLA-IAATSRRKDQAWEFVKFATGPVGQALIGESRLFVPVL 363 >ML1427 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 445 Score = 88.6 bits (218), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 100/356 (28%), Positives = 154/356 (43%), Gaps = 53/356 (14%) Query: 60 LKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGNEAPDL--- 116 L F F +P+E A + F +P+IKV A+ + +++ AG + PD+ Sbjct: 37 LTFFFAANPEETNARMRIVGEFQRDHPDIKVRAV-LSGPGVMQQLSTFCAGGKCPDVLMA 95 Query: 117 -GYMHGELADPWQKDGKFVNMFELLDADK----ELKRSDFLDYIWFKSSEDFAY--GIST 169 + ELAD G +++ LL DK ELK SD ++ ++ E F + G Sbjct: 96 WDLTYAELAD----RGVLLDLNTLLGQDKAFAAELK-SDSIEPLY----ETFTFNGGQYA 146 Query: 170 AGETFG---LFYNKDLFDAAKVEYPPAKPEMAWSWDEFVKVAQQLT-LDKNGKNAADPGF 225 E + LFYNK LF A V+ PP E WS+ EF+ A+ LT D +G+ Sbjct: 147 FPEQWSGNYLFYNKQLFTNAGVQPPPCTWEQPWSFTEFLDTARALTKRDSSGR------- 199 Query: 226 DPDNIKQFGVSFETWHGPVHATV--INNEGEWVT---KDGKFGFSQPEAAEAIQRLADLI 280 + Q+G TW A + +NN W F EA+Q DL Sbjct: 200 ----VTQWGF-VNTWLSYYTAGLFALNNGVPWSNPRMNPTHLNFDDDAFIEAVQFYCDLT 254 Query: 281 NVYHVAPSPVQMKSIPSQAVALQSKLAAMSMHGQW-INLDLGAAKVNYDIGVLP--KMKR 337 N Y VAP + + + + + K AA+++ G W + A +++D+ LP Sbjct: 255 NKYQVAPDASEQQWMATADLFSLGK-AAIALGGHWRYQTFMRAEGLDFDVTSLPIGPSAG 313 Query: 338 SVPMGISGAS--------VIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMPTM 385 +VP SGA I SS +E+AW KF P G + + L++P + Sbjct: 314 TVPATRSGACSDIGATGLAIAASSSRKEQAWEFVKFATGPAGQALIGESCLFVPVL 369 >ML1770 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 446 Score = 87.0 bits (214), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 86/344 (25%), Positives = 150/344 (43%), Gaps = 17/344 (4%) Query: 52 EQSGKPVTLKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVAGN 111 + G V + W + Q A ++ QAFT +P+I+V + + Y + VAG Sbjct: 34 QLRGGKVVVTMRLW-ADQISTAYSQSFQAFTRTHPDIEVHTNVVAYSKYFNTLRTDVAGG 92 Query: 112 EAPDLGYMHGELADPWQKDGKFVNMFELLDADKELKRSDFLDYIWFKSSEDFA-YGISTA 170 A D+ ++ + +G+ +N+ L + SD+ + + + A +G+ Sbjct: 93 SADDIFWLSSAYLAAYADNGRLINISNSL---GQRATSDWEPAVVDQFTRAGALWGVPQL 149 Query: 171 GET-FGLFYNKDLFDAAKVEYPPAKPEMAWSW---DEFVKVAQQLTLDKNGKNAADPGFD 226 + +FYN DL AA ++ P M W+ D + QLTLD NG A PGFD Sbjct: 150 TDAGIAVFYNADLLTAAGID-PVQLNRMQWTSNDDDTLRPLLTQLTLDTNGHVAKTPGFD 208 Query: 227 PDNIKQFGVSFETWHGPVHATVINNEGEWVTKDGKFGFSQPEAAEAIQRLADLINVYHVA 286 ++Q+G + ++ I + G + +F F P A EA + L LIN HVA Sbjct: 209 SRRVRQWGYNAANDPQAIYLNYIGSAGGVFQRGDEFAFDNPSAVEAFRYLVGLINNDHVA 268 Query: 287 P--SPVQMKSIPSQAVALQSKLAAMSMHGQWINLDLGAAKVNYDIGVL---PKMKRSVPM 341 P S S+ L ++A + L A+ ++ I ++ P+ + SV Sbjct: 269 PPASETNNNGDFSRNQFLSGRMALFQSGTYNLALIAREARFHWGIAMMPTGPQGRVSVTN 328 Query: 342 GISGASVIYKSSKHQEEAWLLTKFLENPEGAIDLYKGGLWMPTM 385 GI+ A ++KH + + ++ + EG L + G +P + Sbjct: 329 GIAVAG--NSATKHPDAVRQVLAWMGSREGNAYLGRHGAAVPAV 370 >Rv2318 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 84.3 bits (207), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 67/242 (27%), Positives = 107/242 (44%), Gaps = 13/242 (5%) Query: 50 NAEQSGKPVTLKFTFWGSPQEKAAIEKATQAFTEKYPNIKVDAIHIPEADYDAKITAMVA 109 +AE G + + W P AA ++ AFT +P+I+V + + Y + VA Sbjct: 31 SAEPRGGKIVVTVRLWDEPI-AAAYRQSFAAFTRSHPDIEVRTNLVAYSTYFETLRTDVA 89 Query: 110 GNEAPDLGYMHGELADPWQKDGKFVNMFELLDADKELKRSDFLDYIWFKSSEDFAYGIST 169 G A D+ ++ + G+ + + + AD E D + +G+ Sbjct: 90 GGSADDIFWLSNAYFAAYADSGRLMKI-QTDAADWEPAVVDQF------TRSGVLWGVPQ 142 Query: 170 AGET-FGLFYNKDLFDAAKVEYPPAKPEMAWSW---DEFVKVAQQLTLDKNGKNAADPGF 225 + +FYN DL AA V+ P + WS D + +LT+D +G+ A PGF Sbjct: 143 LTDAGIAVFYNADLLAAAGVD-PTQVDNLRWSRGDDDTLRPMLARLTVDADGRTANTPGF 201 Query: 226 DPDNIKQFGVSFETWHGPVHATVINNEGEWVTKDGKFGFSQPEAAEAIQRLADLINVYHV 285 D ++Q+G + ++ I + G +DGKF F P A EA + L LIN HV Sbjct: 202 DARRVRQWGYNAANDPQAIYLNYIGSAGGVFQRDGKFAFDNPGAIEAFRYLVGLINDDHV 261 Query: 286 AP 287 AP Sbjct: 262 AP 263 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 34,798,092 Number of extensions: 1502867 Number of successful extensions: 8112 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 8115 Number of HSP's successfully gapped: 23 Length of query: 449 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 339 Effective length of database: 25,691,691 Effective search space: 8709483249 Effective search space used: 8709483249 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)