BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0041.p (284 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1893 [T] COG2199 FOG: GGDEF domain 176 3e-44 TP0981_2 [T] COG2199 FOG: GGDEF domain 136 3e-32 aq_035_2 [T] COG2199 FOG: GGDEF domain 131 1e-30 mll3440_3 [T] COG2199 FOG: GGDEF domain 124 2e-28 all2416 [T] COG2199 FOG: GGDEF domain 122 6e-28 >RSc1893 [T] COG2199 FOG: GGDEF domain Length = 533 Score = 176 bits (446), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 106/267 (39%), Positives = 142/267 (53%), Gaps = 5/267 (1%) Query: 19 GWGIVVVSPMALIHQQLIGHIQTIIAYSLIPFIILLSAVILIAHRLARPFAYLADLVSKM 78 GWGIV P L G + +++S+ F++ + + A ++RP LA + Sbjct: 253 GWGIVAQKPTETTLSDLNGLMLVTLSHSIPLFVLSIVFIWWFARSISRPLVELASQARLV 312 Query: 79 GK-ERIVLPPVKPHWSREADLLTKAVFLAVADIQKQTDQLTQEAATDTLTGLKNRRSLEY 137 ER+ W EA L +A+ + +L EA TD LTGL NRR + Sbjct: 313 ATPERLERIKTVSSWYIEAHRLKRALLRGTRLTSTRMARLNLEALTDPLTGLYNRRGVAN 372 Query: 138 TLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGG 197 L PFS + LDVD FK +ND +GH GD+VL+H+A +I R D+ R GG Sbjct: 373 ALRLHAGRHQPFSAVTLDVDHFKRINDRHGHDVGDQVLRHLAALIREQARHGDITGRIGG 432 Query: 198 EEFVILLARTRPAEAFNVAERVRRALEQRKGPIPTQVTVSQGIAHYPQHASEQAELLKKA 257 EEF++LL P AF AER+R A+E R P VTVS G+AHYPQ A E +LK + Sbjct: 433 EEFILLLPDATPDGAFRTAERLRLAVEARPNPAGQVVTVSLGVAHYPQSAGEVDAVLKLS 492 Query: 258 DQALYRAKSNGRNRTVVAE----DNPA 280 D ALY+AK +GRNRTVV + D PA Sbjct: 493 DLALYQAKRDGRNRTVVYQAERADGPA 519 >TP0981_2 [T] COG2199 FOG: GGDEF domain Length = 177 Score = 136 bits (343), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 71/172 (41%), Positives = 108/172 (62%), Gaps = 6/172 (3%) Query: 117 LTQEAATDTLTGLKNRRSLEYTLS---QWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDE 173 L ++A TD +T +K + L+ +++ S+++LD+D FK +NDT+GH+ GD Sbjct: 4 LLEQATTDMMTQMKLKHYFFAMLTAKLDTLSTQETVSVLMLDIDFFKQINDTHGHLCGDL 63 Query: 174 VLKHVAGVIVSSLRPDDVCHRFGGEEFVILLARTRPAEAFNVAERVRRALEQRKGP---I 230 VL+HVA +I S RP D+ R+GGEEF+++L+ EA +VAER+R A EQ P + Sbjct: 64 VLQHVAEIIRSCTRPCDIASRYGGEEFMLMLSNNSSREAAHVAERIRVATEQLTIPYHEV 123 Query: 231 PTQVTVSQGIAHYPQHASEQAELLKKADQALYRAKSNGRNRTVVAEDNPAAP 282 +VTVS G+A Y + L+K+AD ALY+AK NGRN+ V++E N + Sbjct: 124 SIRVTVSAGVAEYLPNQESAETLIKRADSALYQAKQNGRNKVVISEKNMCSS 175 >aq_035_2 [T] COG2199 FOG: GGDEF domain Length = 251 Score = 131 bits (330), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 72/162 (44%), Positives = 102/162 (62%), Gaps = 9/162 (5%) Query: 121 AATDTLTGLKNRRSL----EYTLSQWIASGIPFSLIVLDVDRFKFVNDTYGHVTGDEVLK 176 A TD LTGL NRR E L++ G FS++++D+D FK +NDTYGH GD VLK Sbjct: 84 ALTDPLTGLYNRRVFTEMAEKELAKAKRYGYNFSILMIDIDNFKKINDTYGHDVGDLVLK 143 Query: 177 HVAGVIVSSLRPDDVCHRFGGEEFVILLARTRPAEAFNVAERVRRALEQRKGPIPT---- 232 ++ ++ ++R D+ RFGGEEF+++L+ T A AE++RR +EQ +P Sbjct: 144 KISEILKRNVRGADLVARFGGEEFIVMLSNTNLNGAVKKAEQLRRMIEQTPIELPNGEKL 203 Query: 233 QVTVSQGIAHYPQHASEQAELLKKADQALYRAKSNGRNRTVV 274 +VTVS G++ Y H S + EL+K+ADQALY AK G+NR V Sbjct: 204 RVTVSIGVSTYRGHESLE-ELIKEADQALYEAKRKGKNRVEV 244 >mll3440_3 [T] COG2199 FOG: GGDEF domain Length = 192 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 68/169 (40%), Positives = 103/169 (60%), Gaps = 8/169 (4%) Query: 115 DQLTQEAATDTLTGLKNRRSLEYTLSQWIA----SGIPFSLIVLDVDRFKFVNDTYGHVT 170 + L Q++ D TGL NRR LE T S+ + SG P ++I+LDVD FK NDT+GH Sbjct: 6 ETLRQQSIRDPNTGLYNRRYLEETSSRELRRMERSGQPLAMIMLDVDHFKQFNDTFGHEA 65 Query: 171 GDEVLKHVAGVIVSSLRPDDVCHRFGGEEFVILLARTRPAEAFNVAERVRRALEQ----R 226 GD VLK VA ++ R DV R+GGEEF +++ T A+A AE +R+A++Q Sbjct: 66 GDLVLKQVAATLLDHARESDVVSRYGGEEFALMMPGTSLADAAERAEALRKAIKQLHLTH 125 Query: 227 KGPIPTQVTVSQGIAHYPQHASEQAELLKKADQALYRAKSNGRNRTVVA 275 +G +T S G++ +P+ + E++ AD++LY+AK++GR+R V Sbjct: 126 RGRTLGTITASFGVSAFPELGASWVEIVNAADRSLYQAKADGRDRVVAG 174 >all2416 [T] COG2199 FOG: GGDEF domain Length = 332 Score = 122 bits (306), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 70/174 (40%), Positives = 106/174 (60%), Gaps = 9/174 (5%) Query: 109 DIQKQTDQLTQEAATDTLTGLKNRRSLEYTLSQWI----ASGIPFSLIVLDVDRFKFVND 164 D+ ++ L+ A D+LT L NRR+LE+ L + I + +P SL++LDVD FK VND Sbjct: 144 DLLQKNHILSAIALADSLTELNNRRALEWDLPRQITKARSQNLPLSLVILDVDYFKKVND 203 Query: 165 TYGHVTGDEVLKHVAGVIVSSLRPDDVCHRFGGEEFVILLARTRPAEAFNVAERVRRALE 224 YGH+ GD +L+ + + +LR D R+GGEEFV++LA T EA VA+R+ R + Sbjct: 204 QYGHLIGDRLLQSLCTRLRHNLRSQDTPFRYGGEEFVVILANTTGEEAMIVADRLNRIVG 263 Query: 225 QRKGPIPTQ----VTVSQGIAHYPQHASEQAE-LLKKADQALYRAKSNGRNRTV 273 +++ I ++ VT+S G+A EQ LL +ADQ L AK++GRN+ + Sbjct: 264 EQQFSINSKLMISVTISLGVACLETDDDEQGNSLLHRADQCLLAAKASGRNQVI 317 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,580,682 Number of extensions: 721940 Number of successful extensions: 1801 Number of sequences better than 1.0e-05: 142 Number of HSP's gapped: 1608 Number of HSP's successfully gapped: 142 Length of query: 284 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 179 Effective length of database: 26,268,361 Effective search space: 4702036619 Effective search space used: 4702036619 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)