BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0029.p (539 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 253 7e-67 BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic... 92 3e-18 BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic ... 83 1e-15 BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic... 72 2e-12 BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic ... 69 2e-11 >SP1796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 538 Score = 253 bits (645), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 182/541 (33%), Positives = 277/541 (51%), Gaps = 36/541 (6%) Query: 21 LLLSAVMVMGMAGCSGNKEKADAPADTGDFNKEGLPI-VNEPVTLKVLTVRWGSMGDTFT 79 +LL+A + + +A C G+K A +P D+ EG+ + E TLK +T Sbjct: 11 VLLTASLAV-LAAC-GSKNTASSP----DYKLEGVTFPLQEKKTLKFMTASSPLSPKDPN 64 Query: 80 QNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQTFSDSDIVN--NL 137 + L+ LEK T V IDW S D+AE++++ ++SG LPD + D SD D++N Sbjct: 65 EKLILQRLEKETGVHIDWTNYQS-DFAEKRNLDISSGDLPDAIHNDGA-SDVDLMNWAKK 122 Query: 138 SYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLP-TRLPSRPKSSRQPV--- 193 P+++ ID+YMPNLK ++E PE K + T PDG IYS P K S V Sbjct: 123 GVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHIYSFPWIEELGDGKESIHSVNDM 182 Query: 194 --INKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPYIEVSND-------LI 244 INK WL KLGL+ P T DDL V++AFK DPNGNG+ADEIP+ +S + L Sbjct: 183 AWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNGNGEADEIPFSFISGNGNEDFKFLF 242 Query: 245 SPFGITDLNSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDATMISAK 304 + FGI D + + ++ DGK + ++ YKEG+K+ +L +GL+DKE F D AK Sbjct: 243 AAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVKFIRQLQEKGLIDKEAFEHDWNSYIAK 302 Query: 305 FQNPDAPIVGLSYQWTPDAVFGKWSEQYETIPPVAGPDGKRYTIGNPIGMNLARNELLIT 364 + G+ + W + V G +E Y+ +P +AGP G+++ + GM AR++++IT Sbjct: 303 GHDQK---FGVYFTWDKNNVTGS-NESYDVLPVLAGPSGQKH-VARTNGMGFARDKMVIT 357 Query: 365 TSCKNPEVAARWADQFYTNEASIQNFWGAIGTVLKKN----DDSTYTLMDPPAGTSADAW 420 + KN E+ A+W D Y S+QN WG G ++N D ++ +L P +A A Sbjct: 358 SVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQQNIFELDQASNSLKHLPLNGTAPA- 416 Query: 421 YWDQSLRDFGPKYVSPSFEKNIVLSQSXXXXXXXXXXXXXXXYVT--TPFPNVMYTSEEF 478 Q GP + S+ + Y++ +P V T E+ Sbjct: 417 ELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLDLIKEYYVPYMSNVNNYPRVFMTQEDL 476 Query: 479 QELPTLTTDIDSYVTTMRAQFITKGGIDEGWDAYVKKLNDMGLEKLLQIRTDAYARYMSV 538 ++ + D++ Y+ RA++I G ID WD Y K+L GL L I+ Y +Y + Sbjct: 477 DKIAHIEADMNDYIYRKRAEWIVNGNIDTEWDDYKKELEKYGLSDYLAIKQKYYDQYQAN 536 Query: 539 K 539 K Sbjct: 537 K 537 >BS_lplA [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 502 Score = 91.7 bits (226), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 93/307 (30%), Positives = 156/307 (50%), Gaps = 42/307 (13%) Query: 11 KSRAAKKWPLLLLSAVMVMGMAGCSGNKEKADAPADTGDFNKEGLPIVNEPVTLKVLTVR 70 K R KKW L L+A+M+ AGCS + E +++ + + D K P++ Sbjct: 2 KIRMRKKWMALPLAAMMI---AGCS-HSETSNSASGSKDTIKIMAPLL------------ 45 Query: 71 WGSMGDTFTQNQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPD-VVVGDQTFS 129 S ++ LK LEK T +I + + + ++ +I++ASG +P +V+ D++ Sbjct: 46 --SPESPSDKSPSLKALEKYTGKEIKVTWVPDSSYNDKFNIVMASGEMPHAIVIKDKSAG 103 Query: 130 DSDIVNNLSYFRPLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSRPKSS 189 V +++ L Y+ Y NL A E+ + + +G++Y + +R Sbjct: 104 FIKSVKAGAFWE-LSPYLKDY-KNLSQADEKILKNSSV----NGEVYGI---YRTRDLIR 154 Query: 190 RQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADE----IP-YIEVSNDLI 244 +I WL +GL P+TLDD Y V+KAFKEKDP+GNGK D +P ++ + N Sbjct: 155 ACMIIRTDWLKNVGLDMPETLDDFYEVLKAFKEKDPDGNGKDDTYGMVVPKWMGLGNG-- 212 Query: 245 SPFGITDL---NSNNMLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDATMI 301 SP+ + + N V++GK + T++EY + L + KLY EGL++K+ A M Sbjct: 213 SPWDVLQIWFGAPNRYGVENGKLIPDFTTKEYMDALTFFKKLYDEGLINKDF----AVMD 268 Query: 302 SAKFQNP 308 SAK+ +P Sbjct: 269 SAKWNDP 275 >BH0482 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 512 Score = 83.2 bits (204), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 84/280 (30%), Positives = 139/280 (49%), Gaps = 37/280 (13%) Query: 84 LKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQTFSDSDIVNNL--SYFR 141 +K++E+ TN +D + ++ + LASG LP V+ Q F S ++N F Sbjct: 71 IKEIEELTNTNLDLIWVPDAVKEDRVNAALASGNLPKVITL-QDFRTSSVLNAFRSGQFW 129 Query: 142 PLDEYIDQYMPNLKAAMEETPEMKKISTFPDGKIYSLPTRLPSRPKSSRQPVINKAWLDK 201 + Y+D++ PNL + M ET ++ +S DGKIY + RP S + VI + WL+ Sbjct: 130 EIGPYLDEF-PNL-SEMNETI-LENVSV--DGKIYGI---YRERPLSRQGVVIRQDWLEN 181 Query: 202 LGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPYIEVSNDLISPFG-ITDLNSNN---- 256 LGL+ P L++LY V +AF DPNG+G D + + + NDLI FG L S + Sbjct: 182 LGLEMPTNLEELYEVARAFTYDDPNGSGADDTVGFTD-RNDLI--FGAFKTLASYHGTPN 238 Query: 257 --MLVKDGKAVYYPTSEEYKEGLKWENKLYSEGLLDKELFTQDATMISAKFQNPDAPIVG 314 +GK + ++EY E + + LY EGL++ E +++K Q + + G Sbjct: 239 EWGFDDEGKLMPEFMTDEYVETMNYMKSLYDEGLINTEF------PVTSKEQQQELFMTG 292 Query: 315 LSYQWTPDAVFGK-WSEQYETIPP---------VAGPDGK 344 + + + V + ++ + I P +AGPDG+ Sbjct: 293 RAGIYVGNMVDARNLRDKAKDINPEMEVAITNRIAGPDGE 332 >BS_ytcQ [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 440 Score = 72.0 bits (175), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 97/338 (28%), Positives = 150/338 (44%), Gaps = 45/338 (13%) Query: 23 LSAVMVMG--MAGCSGNKEKADAPADTGDFNKEGLPIVNEPVTLKVLTVRWGSMGDTFTQ 80 + V+ +G +AGC G + + EG + V L + + + + Sbjct: 1 MCLVLALGGVLAGCKGTDQSS----------AEGKAGPDSKVKLSWMAILYHQQP---PK 47 Query: 81 NQWLKDLEKNTNVKIDWQVMSSNDWAEQKSILLASGTLPDVVVGDQTFSDSDIVNNL--S 138 ++ +K++EK TN ++D + ++ + LA+G LP +V Q +S ++N Sbjct: 48 DRAIKEIEKLTNTELDITWVPDAVKEDRLNAALAAGNLPQIVT-IQDIKNSSVMNAFRSG 106 Query: 139 YFRPLDEYIDQYMPNLKAAMEETPEMKKI---STFPDGKIYSLPTRLPSRPKSSRQPVIN 195 F + +YI Y PNL +M K+ + DGK+Y + RP S + VI Sbjct: 107 MFWEIGDYIKDY-PNLN-------KMNKLINKNVTIDGKLYGI---YRERPLSRQGIVIR 155 Query: 196 KAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGNGKADEIPYIEVSNDLISPFGITDLNSN 255 K WLD L LKTP TLD+LY V KAF E DP+ +GK D + NDLI T + Sbjct: 156 KDWLDNLNLKTPKTLDELYEVAKAFTEDDPDKDGKDDTFGLAD-RNDLIYGAFKTIGSYE 214 Query: 256 NMLV--KDGKAVYYP--TSEEYKEGLKWENKLYSEGLLDKELFTQDATMISAKFQNPDAP 311 M K+ + P ++EYK+ + + KL G ++K+ T F A Sbjct: 215 GMPTDWKESGGKFTPDFMTQEYKDTMNYMKKLRDNGYMNKDFPVTSKTQQQELFSQGKA- 273 Query: 312 IVGLSYQWTPDAV-----FGKWSEQYETIPPVAGPDGK 344 G+ DAV S + E I + GPDGK Sbjct: 274 --GIYIGNMVDAVNLRDHASDKSMKLEIINRIKGPDGK 309 >BH1064 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 550 Score = 69.3 bits (168), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 87/319 (27%), Positives = 133/319 (41%), Gaps = 43/319 (13%) Query: 59 NEPVTLKVLTVRWGSMGDTFTQNQWLKD------LEKNTNVKI--DWQVMSSNDWAEQKS 110 NE T+ + T R G F + + D E+ +K +W + + Q Sbjct: 36 NEDGTVTITTARTLDDGTVFKDGEDVHDNVVTRWAEEELGIKFVTEWTQPNDEQFNTQLR 95 Query: 111 ILLASGT-LPDVVVGDQTFSDSDIVNNLSYFRPLDEYIDQYM-PNLKAAMEETPEMKKIS 168 ++++SG LPDV + T +D++++ P+DE I+ Y P LK E+ P+ S Sbjct: 96 LMMSSGEKLPDVFQVNDTAIKADLLSS-GLVMPVDEAIETYASPRLKQLFEQFPQAFYPS 154 Query: 169 TFPDGKIYSLPTRLPSRPKSSRQPVINKAWLDKLGLKTPDTLDDLYNVMKAFKEKDPNGN 228 T DG Y +P R S I + WLDK L+ P TL++L ++M F +DPN Sbjct: 155 T-KDGVRYGIP-RFSGGNGSDTLLWIRQDWLDKFDLEPPTTLEELEHIMDVFVNEDPNET 212 Query: 229 GKADEIPYIEVSNDLISPFG-ITD----LNSNNMLV-------KDGKAVYYPTSEEYKEG 276 GK D I + D G I D + V DG VY K+G Sbjct: 213 GKNDTIGITFAAGDYGFRGGNIADSSWVFGAFGDFVPGAWSKGDDGSLVYGSIQPSMKDG 272 Query: 277 LKWENKLYSEGLLDKELFTQDA-----TMISAKFQNPDAP------IVGLSYQWTPDAVF 325 L ++ Y +G L + A + IS + AP +G Q PDA+ Sbjct: 273 LAKIHEWYEKGYLATDFAILSAGDAQESFISGRSGIMAAPPWAHGYPIGEMAQVVPDAIV 332 Query: 326 GKWSEQYETIPPVAGPDGK 344 + P V+GP+G+ Sbjct: 333 -------KPFPHVSGPNGQ 344 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 41,617,730 Number of extensions: 1819901 Number of successful extensions: 2839 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2830 Number of HSP's successfully gapped: 8 Length of query: 539 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 428 Effective length of database: 25,576,357 Effective search space: 10946680796 Effective search space used: 10946680796 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)