BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0346.0019.p
         (301 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Cj0261c [QR] COG0500 SAM-dependent methyltransferases                  54   3e-07
NMA1127 [J] COG0566 rRNA methylases                                    53   6e-07

>Cj0261c [QR] COG0500 SAM-dependent methyltransferases
          Length = 239

 Score = 53.9 bits (128), Expect = 3e-07,   Method: Compositional matrix adjust.
 Identities = 44/201 (21%), Positives = 90/201 (44%), Gaps = 9/201 (4%)

Query: 63  WLEGQGVDFEGLSVLDVGAASGGFTVPFADRGAKVTAVEPNHPLAGLFIENTARFGPGQV 122
           +L+   + F+  S++D+G  +G +T+  A    ++ A++  + +  +  E+  +     +
Sbjct: 28  YLQNLKISFQDKSIIDIGCGTGVWTLHLAKEAKEILALDSANAMLEILQEDAKKLNLNNI 87

Query: 123 ELVHDLFEHVDLDAKGWKGAFDLVFASMTPVVVDWESVESVLSCARKYCYISLNAGGREF 182
           +  +  FE    +    K  FDL F SM+P + + +   + L+ A+   Y+   A  R+ 
Sbjct: 88  KCKNLSFETWMQNNPNVK--FDLAFLSMSPALQNEKDYTNFLNLAKIKIYLGW-ADYRKS 144

Query: 183 SLLNEVLPLLNGQTLPAKSSDMAYLTHLLYLKGYSFESLITREMKTTELSSGEAIEETLM 242
             L+ +    N +       D   L + L  K   F  ++  E +  + +  EAIE  L 
Sbjct: 145 DFLDPIFKYFNTEFKGFYKKD---LENYLLEKNIFFHKIVFDETRKVQRTKEEAIENALW 201

Query: 243 LLKHHKLPADEAPRRTVTEYV 263
            L  +K+    A + TV+ +V
Sbjct: 202 HLSMNKIT---ASKETVSSFV 219


>NMA1127 [J] COG0566 rRNA methylases
          Length = 275

 Score = 53.1 bits (126), Expect = 6e-07,   Method: Compositional matrix adjust.
 Identities = 38/114 (33%), Positives = 57/114 (50%), Gaps = 4/114 (3%)

Query: 67  QGVDFEGLS-VLDVGAASGGFTVPFADRGAKVTAVEPNHPLAGLFIENTARFGPGQVELV 125
           + +DF   S VLD+GA  G  T+P AD    VTAVEP+  +     EN        V  +
Sbjct: 55  KRIDFTPDSKVLDIGAGPGTLTIPLADMVKHVTAVEPSDGMLYCLKENIKAKELNNVSWI 114

Query: 126 HDLFEHVDLDAKGWKGAFDLVFASMTPVVVDW-ESVESVLSCARKYCYISLNAG 178
              +E  D+D K     +D+V AS +  + D  E+V  + + + KY Y++  AG
Sbjct: 115 QKKWE--DIDLKDLDAPYDVVVASFSLSMPDIKEAVIKMNNVSSKYVYLNWFAG 166


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.319    0.135    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 21,869,936
Number of extensions: 838342
Number of successful extensions: 1611
Number of sequences better than 1.0e-05: 2
Number of HSP's gapped: 1612
Number of HSP's successfully gapped: 2
Length of query: 301
Length of database: 38,378,431
Length adjustment: 106
Effective length of query: 195
Effective length of database: 26,153,027
Effective search space: 5099840265
Effective search space used: 5099840265
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 115 (48.9 bits)