BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0015.p (710 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2123 [G] COG0383 Alpha-mannosidase 733 0.0 BH0791 [G] COG0383 Alpha-mannosidase 670 0.0 TM1851 [G] COG0383 Alpha-mannosidase 316 1e-85 lin2122 [G] COG0383 Alpha-mannosidase 93 2e-18 lin0424 [G] COG0383 Alpha-mannosidase 78 6e-14 >lin2123 [G] COG0383 Alpha-mannosidase Length = 1032 Score = 733 bits (1891), Expect = 0.0, Method: Compositional matrix adjust. Identities = 346/601 (57%), Positives = 432/601 (71%), Gaps = 5/601 (0%) Query: 1 MFWTEEKLGARLRELEGYRYRDVVKLSDWLAVEDKDGANGKYPPQLEGDTRLQLGDYWTG 60 MF+T++KL RL E+ YRY + + A ED DG P + D L +G+ W G Sbjct: 1 MFFTKDKLRGRLDEVALYRYEKTHPIQKFQAAEDMDGNIAARPQNVIYDNELTIGENWAG 60 Query: 61 RDAYLWLHSTIEVPAEWKGKRVVGLFDFGRTGGGNNSGFESLLYLDGKPYQGVDSNHQEV 120 RD Y+WL +TI P+ +G R++G FDFG TG G+NSGFESLL+++G+P+QGVD NHQEV Sbjct: 61 RDRYVWLKTTITFPSAKEGTRLIGYFDFGNTGDGHNSGFESLLFVNGEPFQGVDQNHQEV 120 Query: 121 FLEEDAAGRALDFTFRLWSGLEGGGRPVPQEHRIMRSELAWLDEAADNLYYTGRAALSTC 180 ++ AG+ + FRLWSGLEGGG P Q H++ + + +L+ D+LY+T +A L T Sbjct: 121 LFPDNFAGKTTELVFRLWSGLEGGGVPSIQTHQLKEAFIGYLNLVIDDLYFTSKATLKTL 180 Query: 181 KQLADQQPEKQELLMALNRAFNKLDWSKPGSAGFYRSVRXXXXXXXXXXXXXXXKPPVTV 240 QL + P LL A+NRA+ +DW PGS S+ P+ V Sbjct: 181 DQLEENNPTHAPLLQAINRAYLAIDWGTPGSNQNLSSMENANQILQAAIKEMPKNFPIKV 240 Query: 241 TAIGHTHIDVAWLWRLTHTREKAARSFSTVLRLMKQYPEYIFLQTQPQLYAYIKEDYPEI 300 A+GHTHIDVAWLWRL HTREKAARSFSTVL LM+ YPEY+FLQ+QPQLYAYIKEDYPEI Sbjct: 241 AAVGHTHIDVAWLWRLKHTREKAARSFSTVLHLMEDYPEYLFLQSQPQLYAYIKEDYPEI 300 Query: 301 YAEIRKRVEEGRWEAGGAMWLEADCNLTSGESLVRQILYGTKFFREEFGVDCKYLWLPDV 360 YA+I+ ++EEG WEA G MWLEADCN+ SGESLVRQ+LYG KF REEFG +LWLPDV Sbjct: 301 YAKIKAKIEEGNWEADGGMWLEADCNIPSGESLVRQMLYGQKFLREEFGKPSSFLWLPDV 360 Query: 361 FGYSWALPQILRKSGIDTFMTTKISWSQYNRMPHDTFRWRGIDGSEVLTHFITTPEGPDS 420 FGYSWALPQIL KSGI TFMTTKISW+QYNRMPHDTF+WRGIDG+E+LTHFIT PE Sbjct: 361 FGYSWALPQILCKSGIKTFMTTKISWNQYNRMPHDTFQWRGIDGTEILTHFITAPE---K 417 Query: 421 GAWYYTYNGLIEAFSVQGIWDQYRDKNLNRELLLSXXXXXXXXXVNREMLEMRRRLDKMP 480 + TYNG + A + G+WD+Y+DK +N+ELLL+ VNREMLEMRRR DKMP Sbjct: 418 NSRISTYNGKMTARELVGLWDKYQDKEINQELLLAYGYGDGGGGVNREMLEMRRRYDKMP 477 Query: 481 GIPAVRTGRADEYFERLNEAVRSTDQYVHTWDGELYLEFHRGTYTSQAYNKRMNRKLELL 540 GIP V+ A +YF L+E ++STDQYVHTW+GELY E+HRGTYTSQA+ K+MNRK+EL Sbjct: 478 GIPEVKPKSATDYFNNLHETIQSTDQYVHTWNGELYFEYHRGTYTSQAFTKKMNRKIELG 537 Query: 541 YREAEWLQVLLAVEKGSFSSYPAQLLLEGWHTILRNQFHDIIPGSSIREVYEDSRLEYAE 600 R +EWL VL +++G ++YP + L W +LRNQFHDIIPGSSI+EVY+D+ +EY E Sbjct: 538 LRNSEWLSVLANLKEG--TTYPTEKLANIWQILLRNQFHDIIPGSSIKEVYDDALIEYTE 595 Query: 601 A 601 A Sbjct: 596 A 596 >BH0791 [G] COG0383 Alpha-mannosidase Length = 1039 Score = 670 bits (1729), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/605 (52%), Positives = 425/605 (70%), Gaps = 4/605 (0%) Query: 1 MFWTEEKLGARLRELEGYRYRDVVKLSDWLAVEDKDGANGKYPPQLEGDTRLQLGDYWTG 60 MF+ EKL R+ EL YRYRDV L + E + G P T + D W+G Sbjct: 1 MFYQLEKLQNRINELASYRYRDVKPLDSFYMYEPEQGKESVVPTHYPKQT-FHVNDTWSG 59 Query: 61 RDAYLWLHSTIEVPAEWKGKRVVGLFDFGRTGGGNNSGFESLLYLDGKPYQGVDSNHQEV 120 R+ Y+WL++++ +P W KR V D GRTGGGNNSGFE+LL+++ +P+QGVDSNHQEV Sbjct: 60 RNKYVWLNTSLHLPEAWSDKRCVLFLDVGRTGGGNNSGFEALLFVNKQPFQGVDSNHQEV 119 Query: 121 FLEEDAAGRALDFTFRLWSGLEGGGRPVPQEHRIMRSELAWLDEAADNLYYTGRAALSTC 180 L ++ R L+ +WSGLEGGG P Q +R ++ WLD+ D Y+ +A L Sbjct: 120 LLPKEGGNR-LEIDVMMWSGLEGGGTPRTQYYRFKQAWYGWLDQKVDAFYFQAKAILEAI 178 Query: 181 KQLADQQPEKQELLMALNRAFNKLDWSKPGSAGFYRSVRXXXXXXXXXXXXXXXKPPVTV 240 QL + +L LN+AF ++W++PG+ FY SV V + Sbjct: 179 GQLDVHDATRVAMLSRLNQAFASINWTQPGNEQFYDSVYEALERLERDMEHLPKHSDVHI 238 Query: 241 TAIGHTHIDVAWLWRLTHTREKAARSFSTVLRLMKQYPEYIFLQTQPQLYAYIKEDYPEI 300 AIGH+HID+AWLW+ HT+EKA R+FSTVLRLM YP+Y++LQ+QPQLY ++KE+ P++ Sbjct: 239 HAIGHSHIDLAWLWQTKHTKEKAKRTFSTVLRLMDLYPDYVYLQSQPQLYQWMKEEEPKL 298 Query: 301 YAEIRKRVEEGRWEAGGAMWLEADCNLTSGESLVRQILYGTKFFREEFGVDCKYLWLPDV 360 Y I+++V++G+WE G MW+EADCN+ SGESLVRQ+LYG +FF++EF + K LWLPDV Sbjct: 299 YEAIKQKVDDGQWEVEGGMWVEADCNIPSGESLVRQLLYGKRFFKKEFHKESKILWLPDV 358 Query: 361 FGYSWALPQILRKSGIDTFMTTKISWSQYNRMPHDTFRWRGIDGSEVLTHFITTPE-GPD 419 FGYSWALPQIL+KSGIDTFMTTKISW+QYNRMP+DTF WRG+DG+E+LTHFITTPE G Sbjct: 359 FGYSWALPQILKKSGIDTFMTTKISWNQYNRMPNDTFYWRGLDGTEILTHFITTPEPGRP 418 Query: 420 SGAWYYTYNGLIEAFSVQGIWDQYRDKNLNRELLLSXXXXXXXXXVNREMLEMRRRLDKM 479 +W+YTYNGL+ A +++G W++YRDK +N++LLL+ VNREMLEM+R+LD++ Sbjct: 419 KDSWFYTYNGLMSAETIKGTWEKYRDKAVNQDLLLAYGYGDGGGGVNREMLEMKRQLDQV 478 Query: 480 PGIPAVRTGRADEYFERLNEAVRSTDQYVHTWDGELYLEFHRGTYTSQAYNKRMNRKLEL 539 PG+P V++ EYF +L+E V +T +YVHTWDGELYLE+HRGTYTSQA K+ NR+LE Sbjct: 479 PGVPHVKSTHVKEYFHQLHENVANTAEYVHTWDGELYLEYHRGTYTSQAKMKKKNRQLEF 538 Query: 540 LYREAEWLQVLLAVEKGSFSSYPAQLLLEGWHTILRNQFHDIIPGSSIREVYEDSRLEYA 599 YR+AE L V + EKG + +YP L +GW +L NQFHDIIPGSSI+EVYED+ +Y Sbjct: 539 SYRDAEILAVTASAEKG-WDAYPGSSLEKGWKLLLLNQFHDIIPGSSIKEVYEDAAKDYT 597 Query: 600 EAERI 604 EA I Sbjct: 598 EAAAI 602 >TM1851 [G] COG0383 Alpha-mannosidase Length = 1010 Score = 316 bits (809), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 161/359 (44%), Positives = 221/359 (61%), Gaps = 20/359 (5%) Query: 239 TVTAIGHTHIDVAWLWRLTHTREKAARSFSTVLRLMKQYPEYIFLQTQPQLYAYIKEDYP 298 T+ +GH HID AWLW + T+ K R+F+ + L K YPE+++ Q+ Q+Y +K++ P Sbjct: 247 TIHLVGHAHIDYAWLWPVEETKRKILRTFANSVLLSKLYPEFVYTQSSAQMYEDLKQNSP 306 Query: 299 EIYAEIRKRVEEGRWEAGGAMWLEADCNLTSGESLVRQILYGTKFFREEFGVDCKYLWLP 358 E++ E+RK VEEGRWE G MW+E+DCN+ S ESLVRQ YG KFF EFG K WLP Sbjct: 307 ELFEEVRKLVEEGRWEPVGGMWVESDCNVPSIESLVRQFYYGQKFFEREFGKKSKVCWLP 366 Query: 359 DVFGYSWALPQILRKSGIDTFMTTKISWSQYNRMPHDTFRWRGIDGSEVLTHFITTPEGP 418 DVFG+SW LPQIL+++GI F+TTK++W+ N P+D RWRGIDGSEV+ + + P Sbjct: 367 DVFGFSWVLPQILKEAGIKYFVTTKLNWNDTNEFPYDLCRWRGIDGSEVIYF---SFKNP 423 Query: 419 DSGAWYYTYNGLIEAFSVQGIWDQYRDKNLNRELLLSXXXXXXXXXVNREMLEMRRRLDK 478 + G YNG I+ +V W +R K+L +LLS EMLE L Sbjct: 424 NEG-----YNGKIDPDTVYKTWKNFRQKDLTNRVLLSFGHGDGGGGPTEEMLENYEVLKD 478 Query: 479 MPGIPAVRTGRADEYFERLNEAVRSTDQYVHTWDGELYLEFHRGTYTSQAYNKRMNRKLE 538 PG+P + G +E+F+++ D+ + WDGELYLE HRGTYTSQ K+++++ E Sbjct: 479 FPGLPHLEMGTVEEFFKKV-----EIDEELPVWDGELYLELHRGTYTSQFRTKKLHKEAE 533 Query: 539 LLYREAEWLQVLLAVEKGSFSSYPAQLLLEGWHTILRNQFHDIIPGSSIREVYEDSRLE 597 AE + A FS +L W +LRN+FHDI+PGSSI+EVYED+ E Sbjct: 534 DSLYLAELIS---AFTDKDFSDEIDEL----WKILLRNEFHDILPGSSIKEVYEDTEKE 585 >lin2122 [G] COG0383 Alpha-mannosidase Length = 882 Score = 92.8 bits (229), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 104/389 (26%), Positives = 172/389 (44%), Gaps = 50/389 (12%) Query: 243 IGHTHIDVAWLWRLTHTREKAARSFSTVLRLMKQYPEYIFLQTQPQLYAYIKEDY----P 298 I H+H D W L R + V L+ + + Q+ + EDY P Sbjct: 9 ISHSHWDREWFLPLESLRFRLVTLMDEVEDLLDKEAGFNHFHMDGQMI--MLEDYLAVKP 66 Query: 299 EIYAEIRKRVEEGRWEAGGAMWLEADCNLTSGESLVRQILYGTKFFREEFGVDCKYLWLP 358 ++++ V +G+ G L+ D LTSGE+ +R + YG + EEFG K + P Sbjct: 67 GKREKMKQLVADGKLRIGPWYMLQ-DAFLTSGEANIRNLQYGLEM-AEEFGQAEKIGYFP 124 Query: 359 DVFGYSWALPQILRKSGIDTFMTTK-ISWSQYNRMPHDT--------FRWRGIDGSEVLT 409 D FG +PQ++R++G DT + + ++ + +N D+ W DGS+VL Sbjct: 125 DTFGLYGQVPQLMRQAGFDTVVFGRGVNPTGFNNQVFDSAFASKYSEMFWESPDGSKVLG 184 Query: 410 HFITTPEGPDSGAWYYTYNGL-IEAFSVQGIWDQY-----RDKNLNRELLLSXXXXXXXX 463 + WY N + + + + WD+ R + + L ++ Sbjct: 185 ILLAN--------WYSNGNEIPTDKEAAKIFWDKKLADVERFASTDEWLFMNGCDHQPVQ 236 Query: 464 XVNREMLEMRRRLDKMPGIPAVRTGRADEYFERLNEAVRS--TDQYVHTWDGELYLEFHR 521 E LE+ R L P I + + +FE+ +AV++ + + T GEL + Sbjct: 237 TDLAEALEVAREL--YPEIDFIHS-----HFEKYQDAVKAGLEPEKLQTVHGELTSQQSD 289 Query: 522 G------TYTSQAYNKRMNRKLE-LLYREAEWLQVLLAVEKGSFSSYPAQLLLEGWHTIL 574 G T +S+ Y K+ N K+E LL R AE + V+ A E G SYP + L W ++ Sbjct: 290 GWSTLANTASSRIYLKQANAKIERLLERLAEPISVM-ATEAGV--SYPHEYLAFAWKLLM 346 Query: 575 RNQFHDIIPGSSIREVYEDSRLEYAEAER 603 NQ HD I G S+ EV+ + + + E+ Sbjct: 347 ENQIHDSITGCSLDEVHREMEARFEKVEQ 375 >lin0424 [G] COG0383 Alpha-mannosidase Length = 875 Score = 77.8 bits (190), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 96/390 (24%), Positives = 159/390 (40%), Gaps = 53/390 (13%) Query: 240 VTAIGHTHIDVAWLWRLTHTREKAARSFSTVLRLMKQYPEYIFLQTQPQLYAYIKEDY-- 297 V I H+H D W + + + + V+ ++ Y F Q + + EDY Sbjct: 6 VHVIPHSHWDREWYFTSSRSTIYLVKHLKEVIETLEAKDNYHFYLMDAQ--SSLIEDYLR 63 Query: 298 --PEIYAEIRKRVEEGRWEAGGAMWLEADCNLTSGESLVRQILYGTKFFREEFGVDCKYL 355 PE + K + E R G + + D + S ES+VR +LYGT+ R E G Sbjct: 64 YCPEDKTRLEKLIAEKRL-ITGPWYTQTDQLVISQESIVRNLLYGTRIAR-EMGHSMAVG 121 Query: 356 WLPDVFGYSWALPQILRKSGIDTFMTTKISWSQY--NRMPHDTFRWRGIDGSEVLTHFIT 413 ++PD FG +PQI ++ GI F+ W NR+ F WRG DG+E+L I Sbjct: 122 YVPDAFGQGGNMPQIYKEFGISKFLF----WRGVADNRLKQTEFIWRGDDGTEMLAEQI- 176 Query: 414 TPEG-------PDSGAWYYTY-----NGLIEAFSVQGIWDQYRDKNLNRELLLSXXXXXX 461 P G P++ A TY L E S + Y L++ Sbjct: 177 -PFGYYYGANIPENEAELKTYLDEQIGALEEKASTPNV---YFPNGLDQ----------- 221 Query: 462 XXXVNREMLEMRRRLDKMPGIPAVRTGRADEYFERLNEAVRSTDQYVHTWDGEL----YL 517 V + + E+ + +++ + + +F L + V TD V GEL + Sbjct: 222 -APVRKNLPELVAKFNELDHSREYQIASPETFFADLEKDV--TDLPV--IAGELTEGKHS 276 Query: 518 EFHRGTYTSQAYNKRMNRKLELLYREAEWLQVLLAVEKGSFSSYPAQLLLEGWHTILRNQ 577 H+ ++++A K+ N ++E + L+ +L++ + YP L E W + N Sbjct: 277 RLHKSIFSTRADLKQANNEIENFL--SNVLEPVLSISYSLGNRYPHNELAEIWKLMFENA 334 Query: 578 FHDIIPGSSIREVYEDSRLEYAEAERIGST 607 HD I G + D + Y A + + Sbjct: 335 AHDSIGGCNSDTTNRDVKHRYKLASDLATN 364 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 51,670,026 Number of extensions: 2250124 Number of successful extensions: 3059 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 3046 Number of HSP's successfully gapped: 8 Length of query: 710 Length of database: 38,378,431 Length adjustment: 114 Effective length of query: 596 Effective length of database: 25,230,355 Effective search space: 15037291580 Effective search space used: 15037291580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)