BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0010.p (491 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjmJ [G] COG2721 Altronate dehydratase 603 e-172 uxaA [G] COG2721 Altronate dehydratase 557 e-158 ZuxaA [G] COG2721 Altronate dehydratase 557 e-158 ECs3973 [G] COG2721 Altronate dehydratase 557 e-158 >BS_yjmJ [G] COG2721 Altronate dehydratase Length = 346 Score = 603 bits (1554), Expect = e-172, Method: Compositional matrix adjust. Identities = 0/493 (0%), Positives = 0/493 (0%), Gaps = 2/493 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 + V+ + + E + + V + Sbjct: 5 RIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGL 64 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 + S D + ++ D Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTD 124 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 + V + + L + G + S Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAF 184 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 L I + + + ++ + + V Sbjct: 185 GVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGK 244 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 + + + G + A + +Q + + P + Sbjct: 245 YLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAS 304 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 Sbjct: 305 364 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 Sbjct: 365 424 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLERPMAD--VLEEFIAYIIDVASGQKTRNEQNE 478 Sbjct: 425 484 Query: 479 VRELAIFKTGVTL 491 Sbjct: 485 497 >uxaA [G] COG2721 Altronate dehydratase Length = 346 Score = 557 bits (1436), Expect = e-158, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 1/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 ++ L A D I L AE + G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 + H K ++ + + + + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 ++ + D + Q R A Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 + + S+L + + E + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 N + + L + A + A + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 Sbjct: 304 363 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 Sbjct: 364 423 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 Sbjct: 424 483 Query: 480 RELAIFKTGVTL 491 Sbjct: 484 495 >ZuxaA [G] COG2721 Altronate dehydratase Length = 346 Score = 557 bits (1436), Expect = e-158, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 1/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 ++ L A D I L AE + G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 + H K ++ + + + + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 ++ + D + Q R A Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 + + S+L + + E + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 N + + L + A + A + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 Sbjct: 304 363 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 Sbjct: 364 423 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 Sbjct: 424 483 Query: 480 RELAIFKTGVTL 491 Sbjct: 484 495 Score = 557 bits (1436), Expect = e-158, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 1/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 ++ L A D I L AE + G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 + H K ++ + + + + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 ++ + D + Q R A Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 + + S+L + + E + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 N + + L + A + A + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 Sbjct: 304 363 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 Sbjct: 364 423 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 Sbjct: 424 483 Query: 480 RELAIFKTGVTL 491 Sbjct: 484 495 >ECs3973 [G] COG2721 Altronate dehydratase Length = 346 Score = 557 bits (1436), Expect = e-158, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 1/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 ++ L A D I L AE + G Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 + H K ++ + + + + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 ++ + D + Q R A Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 + + S+L + + E + + Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 N + + L + A + A + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVA 303 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 Sbjct: 304 363 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 Sbjct: 364 423 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 Sbjct: 424 483 Query: 480 RELAIFKTGVTL 491 Sbjct: 484 495 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,419,919 Number of extensions: 1599369 Number of successful extensions: 3198 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 3130 Number of HSP's successfully gapped: 23 Length of query: 491 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 381 Effective length of database: 25,691,691 Effective search space: 9788534271 Effective search space used: 9788534271 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)