BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0009.p (473 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjmI [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 459 e-129 CAC0695 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 452 e-127 YPO0580 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 444 e-124 ECs2128 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 444 e-124 uxaB [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases 440 e-123 >BS_yjmI [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 480 Score = 459 bits (1180), Expect = e-129, Method: Compositional matrix adjust. Identities = 231/473 (48%), Positives = 302/473 (63%), Gaps = 11/473 (2%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 Q+L++ QYPE+++QFGEGNF+R F+DWQ+ Q+NQ FNGS AVV P G Sbjct: 2 QKLNKNVYDHYTQYPEKILQFGEGNFLRGFIDWQIDQLNQHTDFNGSVAVVQPRGSEKIK 61 Query: 63 LMAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNT 122 + EQD LYT+ L G+ + VN II S+SR I+ +SDYEAY LA ++ L FI SNT Sbjct: 62 RLNEQDGLYTLFLQGMKDGEAVNEHMIINSISRGIDLFSDYEAYKELASSERLRFIISNT 121 Query: 123 TEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGK----KGFVIIPCELIDRNGEKLK 178 TEAGI +DRLED P +FP KLTA LY R++ K KG V+IPCELI+ NGEKL+ Sbjct: 122 TEAGIVCDEKDRLEDRPQKTFPGKLTAFLYFRYQAFKGDQTKGCVLIPCELIENNGEKLR 181 Query: 179 EIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTA 238 E V YA W L E F QW+ + NTFC SLVDRIVPG+P E+ A+LGY D L+V Sbjct: 182 ETVLHYAHLWKLEEGFTQWIHEANTFCNSLVDRIVPGFPVDSIDEITADLGYQDDLIVVG 241 Query: 239 EPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGL 298 E + WVIEGP W+ + LP A AGL+ + D+T YR +KV +LNG HTAM P+A++ GL Sbjct: 242 EQYYLWVIEGPDWIGKELPFAAAGLHTKIVSDLTPYRTKKVRILNGAHTAMTPVALLYGL 301 Query: 299 ETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISL 358 +TV D + RF+R L+D+E++PVL + E L YAD VL RFKNP+I+H L SI+L Sbjct: 302 KTVRDAVEHPEVGRFIRELIDDEILPVLKM--EGLSQYADDVLNRFKNPYIKHYLESIAL 359 Query: 359 NSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKRQDSAEVLAVFDQAW 418 N+ISKFKTR LP L Y +++GQLP + +F+ LL Y D QD VL F + W Sbjct: 360 NAISKFKTRNLPTLKEYAEQKGQLPERLVFSFSALLYFYH-DNETLQDDPAVLQFFKEVW 418 Query: 419 SQPS----TFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQ 467 Q + +L E LWG DL ++P LT+ V L+ + + AL+Q Sbjct: 419 CQEDGDMLRIASRVLGEQRLWGADLNEIPKLTDRVAVYLNHIHELGMQRALEQ 471 >CAC0695 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 482 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 220/481 (45%), Positives = 312/481 (64%), Gaps = 15/481 (3%) Query: 4 RLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGL 63 +L+R+ +YPE+++QFGEGNF+RAFVDWQ+ +MN+ FN +V P+ QG+ + Sbjct: 2 KLNRSNFSEFKKYPEKILQFGEGNFLRAFVDWQVDRMNKAADFNAGVVIVQPLEQGLVDM 61 Query: 64 MAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTT 123 + EQD LYT+ L G+ + + V +I V+R +NPY+ YE YL LAEN E+E + SNTT Sbjct: 62 LNEQDGLYTLYLQGLKEGKAVKEHSVIDCVTRGLNPYTQYEEYLKLAENPEMEIVISNTT 121 Query: 124 EAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFEL----GKKGFVIIPCELIDRNGEKLKE 179 EAGIA+ D+L +S+P KLTA LYRRF+ KG +IIPCELIDRNGEKLK Sbjct: 122 EAGIAFDENDKLGRTCQNSYPGKLTAFLYRRFKTFNGDKSKGMLIIPCELIDRNGEKLKA 181 Query: 180 IVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAE 239 + ++A WNL + F W+++ NTFCC+LVDRIVPGYPR E+ AELGY D L+ E Sbjct: 182 TILKFAELWNLEKEFADWIEEANTFCCTLVDRIVPGYPRDTIEEVHAELGYEDSLVDVGE 241 Query: 240 PFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLE 299 F WVIEGP W+ + LP KAG+N+ V D+T YR RKV +LNG HTA+VP+A + GL+ Sbjct: 242 QFHLWVIEGPEWIKDILPFEKAGVNIQVVKDVTPYRTRKVRILNGAHTALVPVAYLYGLD 301 Query: 300 TVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLN 359 TV + D+ +++ L+ +E+IP LDLP+EEL +A AVLERF NPF+ H L SI+LN Sbjct: 302 TVGHSVEDKVIGKYLTELVYDEIIPTLDLPEEELKYFAGAVLERFLNPFVNHYLMSIALN 361 Query: 360 SISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDR----VKRQDSAEVLAVFD 415 S+ KF+TR LP LL Y++ +GQLP + + A L+ Y+G R +K D ++L ++ Sbjct: 362 SMPKFETRDLPSLLEYKKRKGQLPKKLVFSLAALIEFYKGKRGDEDIKLADGEDILELYK 421 Query: 416 QAWSQPSTFTA-------SILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 WS+ A ++L W +L ++ GLT+AV L ++ + A++ + Sbjct: 422 DLWSKFDGTDACYRNIVETVLGYEKNWKMNLNELEGLTDAVTENLIKIDKLGMKEAVKSV 481 Query: 469 V 469 + Sbjct: 482 M 482 >YPO0580 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 483 Score = 444 bits (1142), Expect = e-124, Method: Compositional matrix adjust. Identities = 229/479 (47%), Positives = 313/479 (65%), Gaps = 17/479 (3%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 Q L+R PG +P+++IQFGEGNF+RAFVDWQ+ +N+ N V+ PI Sbjct: 2 QTLNRRDFPG-RSHPDKIIQFGEGNFLRAFVDWQIDLLNEHTDLNAGIVVIRPIDTDFPP 60 Query: 63 LMAEQDNLYTVLLNGIM-QEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSN 121 ++ QD LYT ++ G+ Q + V +I SV+R IN Y ++ YLALA + + F+ SN Sbjct: 61 SLSTQDGLYTAVIRGLNEQGEAVRESRLIRSVNREINIYRQFDDYLALARDANIRFMFSN 120 Query: 122 TTEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFE----LGKKGFVIIPCELIDRNGEKL 177 TTEAGIA+ D+ DAPPSSFPAKLT LL+ RFE KG+V++PCELID NGE L Sbjct: 121 TTEAGIAWNEADQFSDAPPSSFPAKLTRLLFERFEHFDGAADKGWVLLPCELIDYNGEAL 180 Query: 178 KEIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVT 237 +E+V +YA+ W L AF WL + NTFC +LVDRIV GYPR + A L+ ELGY D + T Sbjct: 181 RELVLRYASHWQLPAAFTHWLTENNTFCSTLVDRIVTGYPRDEVAALQTELGYQDSFLDT 240 Query: 238 AEPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAG 297 AE F +VI+GP L++ L L + LNV + DD+ Y+ERKV +LNG HTA+VP+A ++G Sbjct: 241 AEYFYLFVIQGPQGLAQELRLDQLDLNVRIVDDIKPYKERKVAILNGAHTALVPVAYLSG 300 Query: 298 LETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASIS 357 L+TV M+D RFV + + EE++PVLDLP++ELLS++ AVL RF+NPFI+H+L SI+ Sbjct: 301 LDTVGQTMDDAQISRFVEKTITEEIVPVLDLPEDELLSFSQAVLSRFRNPFIQHQLLSIA 360 Query: 358 LNSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKR----QDSAEVLAV 413 LN ++KF+TR+LP LL YQQ++GQLPP +T A A L+ YRG+R + QD A L Sbjct: 361 LNGMTKFRTRILPQLLTYQQQKGQLPPRLTFALAALIAFYRGEREGQTYPLQDDAHWLER 420 Query: 414 FDQAWSQP-------STFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAAL 465 + W+ + +L + + WGQDLT VP L V +L + S RAA+ Sbjct: 421 YSTLWNGVKHGDIALAELVNRVLSDANHWGQDLTAVPQLANQVTEQLQTILSRGMRAAV 479 >ECs2128 [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 483 Score = 444 bits (1141), Expect = e-124, Method: Compositional matrix adjust. Identities = 235/483 (48%), Positives = 312/483 (64%), Gaps = 17/483 (3%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 + L+R PG QYPER+IQFGEGNF+RAFVDWQ+ +N+ N VV PI Sbjct: 2 KTLNRRDFPG-AQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDLNSGVVVVRPIETSFPP 60 Query: 63 LMAEQDNLYTVLLNGIMQE-QPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSN 121 ++ QD LYT ++ G+ ++ + V+ +I SV+R I+ YS+Y+ +L LA N E+ F+ SN Sbjct: 61 SLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEYDEFLKLAHNPEMRFVFSN 120 Query: 122 TTEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGK----KGFVIIPCELIDRNGEKL 177 TTEAGI+Y D+ +DAP S+PAKLT LL+ RF KG++IIPCELID NG+ L Sbjct: 121 TTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGALDKGWIIIPCELIDYNGDAL 180 Query: 178 KEIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVT 237 +E+V +YA W L EAF+QWLD N+FC +LVDRIV GYPR + A+LE ELGY D + T Sbjct: 181 RELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTGYPRDEVAKLEEELGYHDGFLDT 240 Query: 238 AEPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAG 297 AE F +VI+GP L+ L L K LNV++ DD+ Y+ERKV +LNG HTA+VP+A AG Sbjct: 241 AEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVAILNGAHTALVPVAFQAG 300 Query: 298 LETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASIS 357 L+TV + MND FV + + EE+IPVLDLP++EL S+A AV RF+NP+I+H+L SI+ Sbjct: 301 LDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRDELESFASAVTGRFRNPYIKHQLLSIA 360 Query: 358 LNSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKR----QDSAEVLAV 413 LN ++KF+TR+LP LL Q+ G LP +T A A L+ YRG+R QD A L Sbjct: 361 LNGMTKFRTRILPQLLAGQKANGTLPARLTFALAALIAFYRGERNGETYPVQDDAHWLER 420 Query: 414 FDQAWSQ-------PSTFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQ 466 + Q WSQ A +L E W QDLTQVPGL E V L + R A++ Sbjct: 421 YQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREAVR 480 Query: 467 QLV 469 L Sbjct: 481 PLC 483 >uxaB [G] COG0246 Mannitol-1-phosphate/altronate dehydrogenases Length = 483 Score = 440 bits (1131), Expect = e-123, Method: Compositional matrix adjust. Identities = 234/483 (48%), Positives = 311/483 (64%), Gaps = 17/483 (3%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 + L+R PG QYPER+IQFGEGNF+RAFVDWQ+ +N+ N VV PI Sbjct: 2 KTLNRRDFPG-AQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDLNSGVVVVRPIETSFPP 60 Query: 63 LMAEQDNLYTVLLNGIMQE-QPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSN 121 ++ QD LYT ++ G+ ++ + V+ +I SV+R I+ YS+Y+ +L LA N E+ F+ SN Sbjct: 61 SLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEYDEFLKLAHNPEMRFVFSN 120 Query: 122 TTEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGK----KGFVIIPCELIDRNGEKL 177 TTEAGI+Y D+ +DAP S+PAKLT LL+ RF KG++IIPCELID NG+ L Sbjct: 121 TTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGALDKGWIIIPCELIDYNGDAL 180 Query: 178 KEIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVT 237 +E+V +YA W L EAF+QWLD N+FC +LVDRIV GYPR + A+LE ELGY D + T Sbjct: 181 RELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTGYPRDEVAKLEEELGYHDGFLDT 240 Query: 238 AEPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAG 297 AE F +VI+GP L+ L L K LNV++ DD+ Y+ERKV +LNG HTA+VP+A AG Sbjct: 241 AEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVAILNGAHTALVPVAFQAG 300 Query: 298 LETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASIS 357 L+TV + MND FV + + EE+IPVLDL ++EL S+A AV RF+NP+I+H+L SI+ Sbjct: 301 LDTVGEAMNDAEICAFVEKAIYEEIIPVLDLLRDELESFASAVTGRFRNPYIKHQLLSIA 360 Query: 358 LNSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKR----QDSAEVLAV 413 LN ++KF+TR+LP LL Q+ G LP +T A A L+ YRG+R QD A L Sbjct: 361 LNGMTKFRTRILPQLLAGQKANGTLPARLTFALAALIAFYRGERNGETYPVQDDAHWLER 420 Query: 414 FDQAWSQ-------PSTFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQ 466 + Q WSQ A +L E W QDLTQVPGL E V L + R A++ Sbjct: 421 YQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREAVR 480 Query: 467 QLV 469 L Sbjct: 481 PLC 483 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,221,990 Number of extensions: 1507818 Number of successful extensions: 4144 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 4032 Number of HSP's successfully gapped: 47 Length of query: 473 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 363 Effective length of database: 25,691,691 Effective search space: 9326083833 Effective search space used: 9326083833 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)