BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0035.p (305 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0209 [O] COG0265 Trypsin-like serine proteases, typically per... 273 2e-73 TM0209 [G] COG0205 6-phosphofructokinase 273 2e-73 YGR240c [G] COG0205 6-phosphofructokinase 190 2e-48 YMR205c [G] COG0205 6-phosphofructokinase 188 7e-48 alr1913 [G] COG0205 6-phosphofructokinase 170 3e-42 >STM0209 [O] COG0265 Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain Length = 475 Score = 273 bits (698), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 31/305 (10%), Positives = 63/305 (20%), Gaps = 2/305 (0%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 A +A + Q + + P + + T + Sbjct: 16 GLALSPLSATAAETSSSAMTAQQMPSLAPMLEKVMPSVVSINVEGSTTVNTPRMPRNFQQ 75 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKL-SKLGINTMALPGTIDNDISFTD 119 F + + G + + G+ A G + + D Sbjct: 76 FFGDDSPFCQDGSPFQNSPFCQGGGNGGNGGQQQKFMALGSGVIIDAAKGYVVTNNHVVD 135 Query: 120 YTIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEM 179 + K+ + + I ++ I L A V Sbjct: 136 NASVIKVQLSDGRKFDAKVVGKDPRSDIALIQIQNPKNLTAIKLADSDALRVGDYTVAIG 195 Query: 180 PYDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRG 239 A G+ V E + + +L L I Sbjct: 196 NPFGLGETVTSGIVSALGRSGLNVENYENFIQTDAAINRGNSG-GALVNLNGELIGINTA 254 Query: 240 GTPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYEL 299 G + + + + +GEL + +L K + Sbjct: 255 ILAPDGGNIGIGFAIPSNMVKNLTSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRG 314 Query: 300 ASRLS 304 A Sbjct: 315 AFVSQ 319 >TM0209 [G] COG0205 6-phosphofructokinase Length = 319 Score = 273 bits (698), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 147/305 (48%), Positives = 203/305 (66%), Gaps = 2/305 (0%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRDIFPMDLRSVGDIIQRGGTILQSARCLE 60 MNAAVRAVVR + G+EV G++RGY GL++ D ++ + V I ++GGTIL+++RC E Sbjct: 16 MNAAVRAVVRYGVRQGLEVIGVRRGYSGLIDGDFVKLEYKDVAGITEKGGTILRTSRCEE 75 Query: 61 FLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKL-SKLGINTMALPGTIDNDISFTD 119 F T EG++ A + + GI+GLVV GG+GS GA+ L + I + +P TIDNDI TD Sbjct: 76 FKTEEGRELAAKQIKKHGIEGLVVIGGEGSLTGAHLLYEEHKIPVVGIPATIDNDIGLTD 135 Query: 120 YTIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAETILVPEM 179 IG DT + V+DA+ KL+DT SSHER+ IVEVMGRH G IAL AGL +GAE I+VPE+ Sbjct: 136 MCIGVDTCLNTVMDAVQKLKDTASSHERAFIVEVMGRHSGYIALMAGLVTGAEAIIVPEI 195 Query: 180 PYDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALKDRHSSLDARVTVLGHIQRG 239 P D +++ADR+ + RGK +SI+IVAEG VA+ L+ R + R+T+LGH+QRG Sbjct: 196 PVDYSQLADRILEERRRGKINSIIIVAEGAASAYTVARHLEYR-IGYETRITILGHVQRG 254 Query: 240 GTPTPGDRNLASRLGDFAVRSLIEGHSDKACGIIKGELTLTDIDLVVNTKKDFDTELYEL 299 G+PT DR LA +G AV +L++G D + + I ++TKK D +LYE+ Sbjct: 255 GSPTAFDRRLALSMGVEAVDALLDGEVDVMIALQGNKFVRVPIMEALSTKKTIDKKLYEI 314 Query: 300 ASRLS 304 A LS Sbjct: 315 AHMLS 319 >YGR240c [G] COG0205 6-phosphofructokinase Length = 987 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 121/302 (40%), Positives = 176/302 (58%), Gaps = 44/302 (14%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLN--RDIFPMDLRSVGDIIQRGGTILQSARC 58 MNAAVRAVVR+ I+FG +VF + GY+GLL + + M V + GGT++ +AR Sbjct: 220 MNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGTARS 279 Query: 59 LEFLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGAN-----------------KLSK-- 99 +EF EG+++ A L GID LVVCGGDGS GA+ + +K Sbjct: 280 MEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDELVAEGRFTKEE 339 Query: 100 ----LGINTMALPGTIDNDISFTDYTIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMG 155 ++ + L G+IDND+S TD TIG +A+ + + ++ + T SH R+ +VEVMG Sbjct: 340 VAPYKNLSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYIDATAKSHSRAFVVEVMG 399 Query: 156 RHCGDIALHAGLASGAETILVPE--MPY-----DLNEVADRMRDNFARGKRHSIVIVAEG 208 RHCG +AL AG+A+GA+ I +PE +P+ +L EV R R ++G+R++ +IVAEG Sbjct: 400 RHCGWLALMAGIATGADYIFIPERAVPHGKWQDELKEVCQRHR---SKGRRNNTIIVAEG 456 Query: 209 V-------GKGEDVAQALKDRHSSLDARVTVLGHIQRGGTPTPGDRNLASRLGDFAVRSL 261 DV AL + LD +VT+LGH+QRGGT DR LA+ G AV+++ Sbjct: 457 ALDDQLNPVTANDVKDALIEL--GLDTKVTILGHVQRGGTAVAHDRWLATLQGVDAVKAV 514 Query: 262 IE 263 +E Sbjct: 515 LE 516 Score = 81.3 bits (199), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 68/266 (25%), Positives = 119/266 (44%), Gaps = 26/266 (9%) Query: 16 GIEVFGIQRGYQGLLNR-DIFPMDLRSVGDIIQRGGTILQSARCLEFLTPEGQQKGADIL 74 G + + I G+ GL+ ++ + V + GG+ + + R + E A Sbjct: 623 GHKPYAIMNGFSGLIQTGEVKELSWIDVENWHNLGGSEIGTNRSV---ASEDLGTIAYYF 679 Query: 75 NEMGIDGLVVCGGDGSYKGANKLSK-------LGINTMALPGTIDNDISFTDYTIGFDTA 127 + +DGL++ GG ++ +L I +P T+ N++ T+Y++G DT Sbjct: 680 QKNKLDGLIILGGFEGFRSLKQLRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTC 739 Query: 128 VGVVVDAINKLRDTMSSHERSSIV-EVMGRHCGDIALHAGLASGAETILVPEMPYDLNEV 186 + +V+ + ++ + S+ R V EV G H G IA GL +GA ++ PE DL + Sbjct: 740 LNALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLASI 799 Query: 187 ADR---MRDNFARGK---RHSIVIVAEGVGKGEDVAQALKD-----RHSSLDARVTVLGH 235 + +++NF K R+ ++V Q L D R + GH Sbjct: 800 REDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLADIISEASKGKFGVRTAIPGH 859 Query: 236 IQRGGTPTPGDRNLASRLGDFAVRSL 261 +Q+GG P+ DR ASR FAV+ + Sbjct: 860 VQQGGVPSSKDRVTASR---FAVKCI 882 >YMR205c [G] COG0205 6-phosphofructokinase Length = 959 Score = 188 bits (478), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 124/313 (39%), Positives = 173/313 (55%), Gaps = 44/313 (14%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLN------RDIFPMDLRSVGDIIQRGGTILQ 54 MN+ VRA+VRSAI+ G F + GY+GL+ ++ D+R GGT + Sbjct: 211 MNSNVRAIVRSAIFKGCRAFVVMEGYEGLVRGGPEYIKEFHWEDVRGWS---AEGGTNIG 267 Query: 55 SARCLEFLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGA-----------------NKL 97 +ARC+EF EG+ GA L E G+D L+VCGGDGS GA N++ Sbjct: 268 TARCMEFKKREGRLLGAQHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRI 327 Query: 98 SK------LGINTMALPGTIDNDISFTDYTIGFDTAVGVVVDAINKLRDTMSSHERSSIV 151 S +N G+IDND+S TD TIG +A+ + AI+ + T +SH R+ +V Sbjct: 328 SNEQYERMKHLNICGTVGSIDNDMSTTDATIGAYSALDRICKAIDYVEATANSHSRAFVV 387 Query: 152 EVMGRHCGDIALHAGLASGAETILVPEMPYDLNEVADRMRD----NFARGKRHSIVIVAE 207 EVMGR+CG +AL AG+A+ A+ I +PE P +E D+M D + +RGKR +IV+VAE Sbjct: 388 EVMGRNCGWLALLAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAE 447 Query: 208 GVGKGE-------DVAQALKDRHSSLDARVTVLGHIQRGGTPTPGDRNLASRLGDFAVRS 260 G + DV + L DR LD R+T LGH+QRGGT DR LA+ G AV + Sbjct: 448 GAIAADLTPISPSDVHKVLVDRL-GLDTRITTLGHVQRGGTAVAYDRILATLQGLEAVNA 506 Query: 261 LIEGHSDKACGII 273 ++E D +I Sbjct: 507 VLESTPDTPSPLI 519 Score = 89.7 bits (221), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 68/280 (24%), Positives = 134/280 (47%), Gaps = 26/280 (9%) Query: 1 MNAAVRAVVRSAIYFGIEVFGIQRGYQGLLNRD-IFPMDLRSVGDIIQRGGTILQSARCL 59 +N+AV ++ + G + I G+ GL + + ++ + + RGG+ + + R Sbjct: 601 INSAVYSMATYCMSQGHRPYAIYNGWSGLARHESVRSLNWKDMLGWQSRGGSEIGTNR-- 658 Query: 60 EFLTPEGQQKG--ADILNEMGIDGLVVCGGDGSYKGANKLSK-------LGINTMALPGT 110 +TPE G A + DGL++ GG +++ ++L + I + +P T Sbjct: 659 --VTPEEADLGMIAYYFQKYEFDGLIIVGGFEAFESLHQLERARESYPAFRIPMVLIPAT 716 Query: 111 IDNDISFTDYTIGFDTAVGVVVDAINKLRDTMSSHE-RSSIVEVMGRHCGDIALHAGLAS 169 + N++ T+Y++G DTA+ +++ + ++ + SS R+ +V+ G + G +A +A LA Sbjct: 717 LSNNVPGTEYSLGSDTALNALMEYCDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAV 776 Query: 170 GAETILVPE-------MPYDLNEVADRMRDNFARGKRHSIVIVAEGVGKGEDVAQALK-- 220 GA+ VPE + D+ +A RG+ +++ + K + + Sbjct: 777 GAQVSYVPEEGISLEQLSEDIEYLAQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVI 836 Query: 221 --DRHSSLDARVTVLGHIQRGGTPTPGDRNLASRLGDFAV 258 + DA+ GH+Q+GG P+P DR A+R+ AV Sbjct: 837 TAEADGRFDAKPAYPGHVQQGGLPSPIDRTRATRMAIKAV 876 >alr1913 [G] COG0205 6-phosphofructokinase Length = 359 Score = 170 bits (431), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 17/317 (5%), Positives = 46/317 (14%), Gaps = 35/317 (11%) Query: 1 MNAAVRAVVRSA-IYFGIEVFGIQRGYQGLLNRDIFPM-----DLRSVGDIIQRGGTILQ 54 + S + + ++ + Sbjct: 17 LQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVL 76 Query: 55 SARCLEFLTPEGQQKGADILNEMGIDGLVVCGGDGSYKGANKLSKLG-INTMALPGTIDN 113 + ++ ++T + Sbjct: 77 LLEGFFDARDLPTISAQ--HFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRP 134 Query: 114 DISFTDYTIGFDTAVGVVVDAINKLRDTMSSHERSSIVEVMGRHCGDIALHAGLASGAET 173 + + Y I + + + + G S A + Sbjct: 135 EQAEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAAS 194 Query: 174 ILVPEMPYDLNEVADR---MRDNFARGKRHSIVIVAEG--------------------VG 210 + P + + G Sbjct: 195 GGILLWPQSHFDWVRPGIILYGVSPL--EDRSTGADFGCQPVMSLTSSLIAVREHKAGEP 252 Query: 211 KGEDVAQALKDRHSSL-DARVTVLGHIQRGGTPTPGDRNLASRLGDFAVRSLIEGHSDKA 269 G + A G+ + + TP N V + Sbjct: 253 VGYGGTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGP 312 Query: 270 CGIIKGELTLTDIDLVV 286 K + + Sbjct: 313 QAQDKAGDPVILWGEGL 329 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,028,294 Number of extensions: 962775 Number of successful extensions: 2135 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 2090 Number of HSP's successfully gapped: 19 Length of query: 305 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 199 Effective length of database: 26,153,027 Effective search space: 5204452373 Effective search space used: 5204452373 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)