BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0033.p (454 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yojI [V] COG0534 Na+-driven multidrug efflux pump 445 e-125 BH0860 [V] COG0534 Na+-driven multidrug efflux pump 444 e-124 lin1960 [V] COG0534 Na+-driven multidrug efflux pump 344 2e-94 SP1166 [V] COG0534 Na+-driven multidrug efflux pump 271 2e-72 STM1425 [V] COG0534 Na+-driven multidrug efflux pump 261 2e-69 >BS_yojI [V] COG0534 Na+-driven multidrug efflux pump Length = 452 Score = 445 bits (1145), Expect = e-125, Method: Compositional matrix adjust. Identities = 224/450 (49%), Positives = 299/450 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T + ++K +Q L+IL PI ITQ LS ITF DT MSGK DLAGVAIG+SLW P+ Sbjct: 1 MKETQNAREKIKQLLHILIPIFITQAGLSLITFLDTVMSGKVSPADLAGVAIGSSLWTPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM +TP+V+ L+G++K+ + + V Q ++ G A+ +L + Sbjct: 61 YTGLAGILMAVTPIVAQLLGAEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRL 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 NL+ VH IA FL +S+GI PLF YTV+RS ID+LG+TRV+M ITL +LP+N LNY+ Sbjct: 121 NLDIHVHQIAKHFLGFLSLGIFPLFVYTVLRSFIDSLGKTRVTMMITLSSLPINFVLNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 IFGKFG P LGGVGAG+ASA+TYW I I+ +++ P K Sbjct: 181 FIFGKFGMPALGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKN 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LLKIG+PIGF++FFET++F+AVTLLMS F T+TIA+HQAA+NFAS LYM+PLS+ M+LTI Sbjct: 241 LLKIGLPIGFAVFFETSIFAAVTLLMSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 +VGFE GA R KDAR Y ++GI +AG+Y+ +P+V+ L +HF Sbjct: 301 VVGFEAGAARFKDARSYSLIGIMMAIGFSLFTAACILLFREQIAGMYTSDPDVLRLTQHF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LIYA+FFQ+SDA+A P QG LRGYKDVN F++YWVIGLP GY++ T+TSL A+GY Sbjct: 361 LIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWVIGLPVGYMVGTFTSLGAFGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHNK 450 W+ RL K+Q++Y+ + Sbjct: 421 WIGLIAGLAAGAVGLFFRLAKLQKRYSQKQ 450 >BH0860 [V] COG0534 Na+-driven multidrug efflux pump Length = 458 Score = 444 bits (1141), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/448 (50%), Positives = 305/448 (68%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M PT ++++K + FL ++ PILITQI L A+ FFDT MSG+ G +DLAGVAIG+SLW+P+ Sbjct: 1 MKPTETLQEKGKLFLVVMMPILITQIGLYAMNFFDTVMSGQAGANDLAGVAIGSSLWVPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+G+L+ +TP+++ +G++K DV Y QG++ G L P+L M Sbjct: 61 FTGLNGVLLALTPIIAQSIGAEKRDDVPYVFLQGLYLSIAISIAVILIGAVVLDPILSAM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 +LE +V IA +L ++ GI+PLF YT IR ID+LG+TRV+MFITL++LP+N+ NY+ Sbjct: 121 SLEDEVGRIAKEYLIGLAFGIVPLFIYTTIRCLIDSLGETRVTMFITLLSLPINIFFNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFGK GFPRLGGVGAG ASAITYW I +A++ V + P KE Sbjct: 181 LIFGKLGFPRLGGVGAGYASAITYWFILAVAIVVVVKVRPFTDFQLFKKLYHVSLKKWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 +L +G+PIGF+IFFET++F+AVTLLMS FDT TIAAHQAA+NFAS LYMIPLSI +LTI Sbjct: 241 ILLLGLPIGFTIFFETSIFAAVTLLMSTFDTATIAAHQAAVNFASFLYMIPLSIAFTLTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VG+E GA R++DARQY +GI + VA LY+++ +V LI+ F Sbjct: 301 AVGYEVGAKRVEDARQYSRLGITFALIMGLVAGVIIYVLRAPVASLYTNDSQVAWLIQQF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LIY+IFFQ+SDA+ATP QGVLRG+KDVN F++ +++W+IGLPTGY+LA ++ L YGY Sbjct: 361 LIYSIFFQLSDALATPIQGVLRGHKDVNVPFVMALVSFWIIGLPTGYLLANFSPLGPYGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAH 448 W+ RL ++QR++ Sbjct: 421 WIGLITGLASCAIALSWRLKQMQRKFER 448 >lin1960 [V] COG0534 Na+-driven multidrug efflux pump Length = 1091 Score = 344 bits (882), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 23/449 (5%), Positives = 65/449 (14%), Gaps = 1/449 (0%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M + T ++ T + F + + Sbjct: 1 MHRYVQKDIQGTTEPQFRNSHSRTSGTVTLYLPVSTFVGTGFFIHPQSKEKLMKRHLNTC 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 + + G + S L ++ + + + Sbjct: 61 YRLVWNHMTGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPGETVNGGTL 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 IS G+ + V Sbjct: 121 ANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQRVNPGGS 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 + + + + + Sbjct: 181 VSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGTVATDTVV 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 + + + +Y + Sbjct: 241 NTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYAGGDQTVHGHAL 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 G + + + + G + + Sbjct: 301 DTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRLQVDAGGTATNVTL 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 + A T + A + + + TG+ T T + G Sbjct: 361 KQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLTGHTA-TNTRVDDGGT 419 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHN 449 ++ A + Sbjct: 420 LDVRNGGTATTVSMGNGGVLLADSGAAVS 448 >SP1166 [V] COG0534 Na+-driven multidrug efflux pump Length = 453 Score = 271 bits (692), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 160/422 (37%), Positives = 248/422 (58%), Gaps = 1/422 (0%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T +++K FL I FPILI Q A + +F DT M+G++ T DLAGV++ TS+W P Sbjct: 1 MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNTMDLAGVSMATSIWNPF 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 T L+GI+ + P++ H +G K+ +VA Q I+ F +L+ + Sbjct: 61 FTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLSVVLLGMVLFLAPIILNHI 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 LE V +A R+L +S+GIIPL ++VIRS +D+LG T++SM++ L+ LP+N G NYL Sbjct: 121 GLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLTKLSMYLMLLLLPLNSGFNYL 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LI+G FG P LGG GAG+ +++ YWV+ GI+++ +++ E KE Sbjct: 181 LIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQEK-LKALHLEKRIPLNMDKIKE 239 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 +++G+PIG ++F E A+FS V L+M++F + IA+HQ+A+NF+S +Y P+SI ++ I Sbjct: 240 GVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASHQSAMNFSSLMYAFPMSISSAMAI 299 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 +V +E GA R DA+ Y +G +VA LY ++P+ I L F Sbjct: 300 VVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTFLYIFRGNVASLYGNDPKFIDLTVRF 359 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 L Y++FFQ++D A P QG+LRGYKD F + + YW + +P + + T AY Y Sbjct: 360 LTYSLFFQLADTFAAPLQGILRGYKDTVIPFYLGLLGYWGVAIPVRTLFDSLTDFGAYSY 419 Query: 421 WV 422 W+ Sbjct: 420 WI 421 >STM1425 [V] COG0534 Na+-driven multidrug efflux pump Length = 457 Score = 261 bits (666), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 146/440 (33%), Positives = 231/440 (52%), Gaps = 4/440 (0%) Query: 10 KARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPIQTGLSGILM 69 +ARQ L + P+++ Q+A +A+ F DT M+G + D+A VAIGTS+W+P G+L+ Sbjct: 7 EARQLLALRIPVILAQVAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLL 66 Query: 70 GITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFMNLEPQVHDI 129 +TP+++ L GS + +A+QV QG W A + N++P + D Sbjct: 67 ALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLVMIVLWNAGYIIRSMHNIDPALADK 126 Query: 130 AFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYLLIFGKFGFP 189 A +L A+ G + V R+ + L +T+ M + + L VN+ +NY+ I+G FG P Sbjct: 127 AVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFLGLLVNIPVNYIFIYGHFGMP 186 Query: 190 RLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKELLKIGVPIG 249 LGG+G GVA+A YWV+F L ++ K L+++G+PI Sbjct: 187 ELGGIGCGVATAAVYWVMFIAMLSYIKHARSMRDIRNEKGFGKPDSVVMKRLIQLGLPIA 246 Query: 250 FSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTILVGFENGAG 309 ++FFE +F+ V LL+S + +A HQ A+NF+S ++++P+S+ ++TI VG+ G G Sbjct: 247 LALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQG 306 Query: 310 RLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHFLIYAIFFQI 369 DA+ G+G H+A LY+D PEV++L ++ A +QI Sbjct: 307 STLDAQTAARTGLGVGICMAVVTAIFTVTLRKHIALLYNDNPEVVALAAQLMLLAAVYQI 366 Query: 370 SDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLA----TYTSLAAYGYWVXXX 425 SD+I G+LRGYKD F I F AYWV+GLP+GY+LA + G+W+ Sbjct: 367 SDSIQVIGSGILRGYKDTRSIFFITFTAYWVLGLPSGYILALTDLVVDRMGPAGFWMGFI 426 Query: 426 XXXXXXXXXXXSRLVKVQRQ 445 R+ +QRQ Sbjct: 427 IGLTSAAVLMMLRMRYLQRQ 446 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.143 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,154,884 Number of extensions: 1142140 Number of successful extensions: 2741 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 2608 Number of HSP's successfully gapped: 63 Length of query: 454 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 344 Effective length of database: 25,691,691 Effective search space: 8837941704 Effective search space used: 8837941704 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)