BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0030.p (257 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yitT [S] COG1284 Uncharacterized conserved protein 167 2e-41 BH2916 [S] COG1284 Uncharacterized conserved protein 149 4e-36 lin2365 [S] COG1284 Uncharacterized conserved protein 130 3e-30 SP2113 [S] COG1284 Uncharacterized conserved protein 106 3e-23 SPy2155 [S] COG1284 Uncharacterized conserved protein 101 1e-21 >BS_yitT [S] COG1284 Uncharacterized conserved protein Length = 280 Score = 167 bits (422), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 90/261 (34%), Positives = 145/261 (55%), Gaps = 8/261 (3%) Query: 3 ACGFNLFLIPHRLLSGGVSGLAML----VGYFTPFNISXXXXXXXXXXXVA--GWFQLGR 56 A G NLFLIP + + G +G+A L V + PF IS V GW ++G+ Sbjct: 20 AAGLNLFLIPADVYASGFTGVAQLLSSVVDQYAPFYISTGTLLFLLNIPVGILGWLKVGK 79 Query: 57 RFIVMSILSVGATTWLMALIPTATVASDMLXXXXXXXXXXXXXXXXXXXXXXXXXXFDIL 116 F V SILSV TT M ++P +++ D+L DI+ Sbjct: 80 SFTVYSILSVALTTLFMGILPETSLSHDILLNAVFGGVISAVGIGLTLKYGASTGGLDIV 139 Query: 117 GSIITRYRDFPIGNVLVGMNALVILAAAYFDNNWNLALASMVSIYVSGRVVDLIHISHIK 176 ++ +++D P+G +N ++IL A W AL ++V++YV+ RV+D IH H+K Sbjct: 140 AMVLAKWKDKPVGTYFFILNGIIILTAGLL-QGWEKALYTLVTLYVTTRVIDAIHTRHMK 198 Query: 177 VTVYIVTTRTDELLKQLL-VLQRGVTKIKTEGAYSHIERDMLMTVTTRYELAELKRIIKN 235 +T IVT + DE+ + + + RG+T + +GA+++ +++M++ V TRYEL +L++I+K Sbjct: 199 LTAMIVTKKADEIKEAIYGKMVRGITTVPAKGAFTNEQKEMMIIVITRYELYDLEKIVKE 258 Query: 236 SDPQAFVNIVETVGVMGSFRK 256 DP+AF NIV+T G+ G FRK Sbjct: 259 VDPKAFTNIVQTTGIFGFFRK 279 >BH2916 [S] COG1284 Uncharacterized conserved protein Length = 293 Score = 149 bits (376), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 82/257 (31%), Positives = 134/257 (52%), Gaps = 4/257 (1%) Query: 2 IACGFNLFLIPHRLLSGGVSGLAMLVGYFTPFNISXXXXXXXXXXXVAGWFQLGRRFIVM 61 +A N FLIP + + G +G+A ++ P + + GW +LG+ F V Sbjct: 38 LAASLNFFLIPADVFASGFTGVAQILATLLPLSTGILLLLLNIPVAIIGWLKLGKAFTVY 97 Query: 62 SILSVGATTWLMALIPTATVASDMLXXXXXXXXXXXXXXXXXXXXXXXXXXFDILGSIIT 121 S LSV TT + + P + D+L DI+ +++ Sbjct: 98 SFLSVALTTLFLEVFPVQAFSPDILLNAVFGGVVAAIGAGITLKVGASSGGLDIIALLLS 157 Query: 122 RYRDFPIGNVLVGMNALVILAAAY-FDNNWNLALASMVSIYVSGRVVDLIHISHIKVTVY 180 R D P+G +N L+++A+ + FD AL ++V++YV+ R++D IH H+K+T Sbjct: 158 RVSDRPVGIYFFLLNGLIVVASGFLFDAEK--ALYTLVTLYVTSRIIDAIHTRHVKLTAM 215 Query: 181 IVTTRTDELLKQLLV-LQRGVTKIKTEGAYSHIERDMLMTVTTRYELAELKRIIKNSDPQ 239 IVT + EL K + + RG+T++ +G Y ++++LM V TRYEL +L+ +++ DP+ Sbjct: 216 IVTKKPTELRKAIHERITRGITRMPAKGGYLEDDKEVLMIVLTRYELYDLRHVVQEVDPE 275 Query: 240 AFVNIVETVGVMGSFRK 256 AF NIV T GV G FRK Sbjct: 276 AFTNIVHTEGVYGLFRK 292 >lin2365 [S] COG1284 Uncharacterized conserved protein Length = 300 Score = 130 bits (326), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 81/262 (30%), Positives = 130/262 (49%), Gaps = 8/262 (3%) Query: 3 ACGFNLFLIPHRLLSGGVSGLAMLVGYF--TPFNISXXXXXXXXXXXVA----GWFQLGR 56 A G N FLIP ++ + G++G+A L NIS + GW ++G+ Sbjct: 25 AIGMNFFLIPAQVYAAGLNGVAQLGSDMLRDTMNISISTGLLVLLLNIPVAILGWLKVGK 84 Query: 57 RFIVMSILSVGATTWLMALIPTATVASDMLXXXXXXXXXXXXXXXXXXXXXXXXXXFDIL 116 F V S L+V ++ + +IP V++D+L DIL Sbjct: 85 SFTVFSFLTVAFMSFFLIVIPEVQVSNDILLNAIFGALIASVGVGLALKFGISTGGLDIL 144 Query: 117 GSIITRYRDFPIGNVLVGMNALVILAAAYFDNNWNLALASMVSIYVSGRVVDLIHISHIK 176 IT G + +N ++I+ A F +W AL +++S+YV RV+D+IH H K Sbjct: 145 AMYITIKTGRSFGKYFLLLNGIIIIVAG-FAYDWTFALYTLISLYVQSRVIDIIHTRHQK 203 Query: 177 VTVYIVTTRTDELLKQLLV-LQRGVTKIKTEGAYSHIERDMLMTVTTRYELAELKRIIKN 235 +TV I+T ++ ++K + + RG+T + G YS + ML+ V TRYEL ++ I+ Sbjct: 204 LTVMIMTQHSETVIKAIHENMVRGITVVDAMGGYSKQDVAMLIMVITRYELYDITHIVGE 263 Query: 236 SDPQAFVNIVETVGVMGSFRKR 257 DP+AF+N++ET V G FR Sbjct: 264 FDPKAFINVMETSSVFGDFRSE 285 >SP2113 [S] COG1284 Uncharacterized conserved protein Length = 313 Score = 106 bits (265), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 71/262 (27%), Positives = 121/262 (46%), Gaps = 13/262 (4%) Query: 3 ACGFNLFLIPHRLLSGGVSGLAMLV--------GYFTPFNISXXXXXXXXXXXVAGWFQL 54 A N F P + S G +GLA ++ G+ P IS V W+Q+ Sbjct: 50 AVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIP--ISLSFYAINFPLMVLAWYQI 107 Query: 55 GRRFIVMSILSVGATTWLMALIPTATVASDMLXXXXXXXXXXXXXXXXXXXXXXXXXXFD 114 G +F V + ++V +++ + +P AT+ D + D Sbjct: 108 GHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNNISSGGTD 167 Query: 115 ILGSIITRYRDFPIGNVLVGMNALVILAAAYFDNNWNLALASMVSIYVSGRVVDLIHISH 174 I+ I + +G++ +N ++L A W AL SM++I+VS RV D + Sbjct: 168 IVSLTIRKKTGKNVGSISFLVNGTIMLIAG-LTFGWKYALYSMITIFVSSRVTDAVFTKQ 226 Query: 175 IKVTVYIVTTRTDELLKQL-LVLQRGVTKIK-TEGAYSHIERDMLMTVTTRYELAELKRI 232 ++ IVT +++++++ L RG T I EG Y+H + +L+TV TR E E K+I Sbjct: 227 KRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVITRAEFNEFKQI 286 Query: 233 IKNSDPQAFVNIVETVGVMGSF 254 + DP +FV++ E V ++G F Sbjct: 287 MTQVDPSSFVSVSENVHILGRF 308 >SPy2155 [S] COG1284 Uncharacterized conserved protein Length = 313 Score = 101 bits (252), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 73/258 (28%), Positives = 121/258 (46%), Gaps = 13/258 (5%) Query: 7 NLFLIPHRLLSGGVSGLAM--------LVGYFTPFNISXXXXXXXXXXXVAGWFQLGRRF 58 N F P + S G +GLA L+GY F I+ + W+++G +F Sbjct: 55 NFFFQPGHVYSSGATGLAQVFSALSHRLLGY--DFPIAFAFYLINIPLLILAWYKIGHQF 112 Query: 59 IVMSILSVGATTWLMALIPTATVASDMLXXXXXXXXXXXXXXXXXXXXXXXXXXFDILGS 118 + + ++V +++ + ++P T+ +D L DI+ Sbjct: 113 TIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKSRISSGGTDIVSL 172 Query: 119 IITRYRDFPIGNVLVGMNALVILAAAYFDNNWNLALASMVSIYVSGRVVDLIHISHIKVT 178 + + +G++ + +N ILA A W AL SMVSI+VS RV D I K+ Sbjct: 173 TLRKRTGKDVGSLSLMVNG-AILAFAGILFGWQYALYSMVSIFVSSRVTDAIFTKQKKMQ 231 Query: 179 VYIVTTRTDELLKQLLV-LQRGVTKIK-TEGAYSHIERDMLMTVTTRYELAELKRIIKNS 236 IVT+ + ++ + L RGVT I EG Y H ++ +L+T+ T E E K ++ + Sbjct: 232 ATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITILTCEEYPEFKWLMLKT 291 Query: 237 DPQAFVNIVETVGVMGSF 254 DPQAFV++ E V ++G F Sbjct: 292 DPQAFVSVAENVRIIGRF 309 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.141 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,127,789 Number of extensions: 506396 Number of successful extensions: 1119 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 1078 Number of HSP's successfully gapped: 29 Length of query: 257 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 153 Effective length of database: 26,383,695 Effective search space: 4036705335 Effective search space used: 4036705335 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 114 (48.5 bits)