BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0029.p (555 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydbR [LKJ] COG0513 Superfamily II DNA and RNA helicases 548 e-156 lin0859 [LKJ] COG0513 Superfamily II DNA and RNA helicases 531 e-150 BH2384 [LKJ] COG0513 Superfamily II DNA and RNA helicases 472 e-133 SP1586 [LKJ] COG0513 Superfamily II DNA and RNA helicases 460 e-129 L0339 [LKJ] COG0513 Superfamily II DNA and RNA helicases 452 e-127 >BS_ydbR [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 511 Score = 548 bits (1413), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/448 (59%), Positives = 346/448 (77%), Gaps = 2/448 (0%) Query: 1 MNNLK-TFAEFGLEPKVLQAITELGFEEATPIQEQAIPIALTGSDLIGQAQTGTGKTAAF 59 +NNL TF +F L +++AI +GFEEATPIQ Q IP+ L+ D+IGQAQTGTGKTAAF Sbjct: 15 LNNLTITFQDFNLSSDLMKAINRMGFEEATPIQAQTIPLGLSNKDVIGQAQTGTGKTAAF 74 Query: 60 GIPLISKIAREEEKILALVMTPTRELAIQVAEEIGKLTRFKGLRSLAIYGGQDIGRQIRG 119 GIPL+ KI E I A+V+ PTRELAIQV+EE+ K+ + K + L IYGGQDIGRQIR Sbjct: 75 GIPLVEKINPESPNIQAIVIAPTRELAIQVSEELYKIGQDKRAKVLPIYGGQDIGRQIRA 134 Query: 120 LKKKPQIIIGTPGRLLDHINRKTIRLDDVQTIVLDEADEMLDMGFMEDIQTILKLVPEER 179 LKK P II+GTPGRLLDHINR+TIRL++V T+V+DEADEML+MGF++DI++IL VP E Sbjct: 135 LKKNPNIIVGTPGRLLDHINRRTIRLNNVNTVVMDEADEMLNMGFIDDIESILSNVPSEH 194 Query: 180 QTMLFSATMPPNIQRLAQQFLKNPQHVSVIPKQISAPLIDQAYIEVPERQKFEALSRLID 239 QT+LFSATMP I+R+A++F+ P+HV V K+++ I Q Y+EV ER+KF+ L+RL+D Sbjct: 195 QTLLFSATMPAPIKRIAERFMTEPEHVKVKAKEMTVSNIQQFYLEVQERKKFDTLTRLLD 254 Query: 240 MESPDLAIVFGRTKRRVDELAEGLQKRGYSADGLHGDLSQNQRDAVMRKFRDGSIDVLVA 299 ++SP+LAIVFGRTKRRVDELAE L RGY+A+G+HGDL+Q +R +RKF++G+I+VLVA Sbjct: 255 IQSPELAIVFGRTKRRVDELAEALNLRGYAAEGIHGDLTQAKRMVALRKFKEGAIEVLVA 314 Query: 300 TDVAARGLDVSGVTHVINFDLPQDPESYVHRIGRTGRAGKEGTAWSFVTPREMDHLHLIE 359 TDVAARGLD+SGVTHV NFD+PQDPESYVHRIGRTGRAGK G A +F+TPRE L IE Sbjct: 315 TDVAARGLDISGVTHVYNFDVPQDPESYVHRIGRTGRAGKTGMAMTFITPREKSMLRAIE 374 Query: 360 RVTRHRITRKPLPTMAEAIEGKQRITAERLLAMVEGGGELNEYKGIAIQLLEQYDSVQLL 419 + T+ ++ R PT+ EA+EG+Q++T ERL + LN Y A +LLE +D+V ++ Sbjct: 375 QTTKRKMDRMKEPTLDEALEGQQQVTVERLRTTI-SENNLNFYMTAAAELLEDHDAVTVV 433 Query: 420 TAAMKLLTGDTKDAQVELTPEDPIRVKR 447 AA+K+ T + D V LT E P+ KR Sbjct: 434 AAAIKMATKEPDDTPVRLTDEAPMVSKR 461 >lin0859 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 516 Score = 531 bits (1367), Expect = e-150, Method: Compositional matrix adjust. Identities = 253/441 (57%), Positives = 346/441 (78%), Gaps = 3/441 (0%) Query: 4 LKTFAEFGLEPKVLQAITELGFEEATPIQEQAIPIALTGSDLIGQAQTGTGKTAAFGIPL 63 + F+EFGL+ K+++++ +GFEEATPIQE+ IP+ L G DLIGQAQTGTGKTAAFG+P+ Sbjct: 1 MTKFSEFGLDEKIVKSVNRMGFEEATPIQEKTIPLGLAGKDLIGQAQTGTGKTAAFGLPM 60 Query: 64 ISKIAREEEKILALVMTPTRELAIQVAEEIGKLTRFKGLRSLAIYGGQDIGRQIRGLKKK 123 I KI ++ + AL++ PTRELAIQV+EE+ KL+ K +R LA+YGG DI RQIR LKK Sbjct: 61 IHKIDQKSNNVQALIIAPTRELAIQVSEELYKLSYDKHVRVLAVYGGSDISRQIRSLKKN 120 Query: 124 PQIIIGTPGRLLDHINRKTIRLDDVQTIVLDEADEMLDMGFMEDIQTILKLVPEERQTML 183 PQI++GTPGR+LDHINR+T++LD V+T+VLDEADEML+MGF++DI+TILK VP ERQT+L Sbjct: 121 PQIVVGTPGRILDHINRRTLKLDHVETLVLDEADEMLNMGFIDDIETILKEVPAERQTLL 180 Query: 184 FSATMPPNIQRLAQQFLKNPQHVSVIPKQISAPLIDQAYIEVPERQKFEALSRLIDMESP 243 FSATMP I+R+ ++F+ +P+ + + K+++A LI+Q +++V E++KF+ LSRL+D+++P Sbjct: 181 FSATMPDPIRRIGERFMHSPELIRIKAKEMTALLIEQFFVKVHEKEKFDVLSRLLDVQAP 240 Query: 244 DLAIVFGRTKRRVDELAEGLQKRGYSADGLHGDLSQNQRDAVMRKFRDGSIDVLVATDVA 303 +LAIVFGRTKRRVDEL+ L RGY A+G+HGDL+Q +R +V+RKF++G IDVLVATDVA Sbjct: 241 ELAIVFGRTKRRVDELSRALDMRGYVAEGIHGDLTQAKRMSVLRKFKEGKIDVLVATDVA 300 Query: 304 ARGLDVSGVTHVINFDLPQDPESYVHRIGRTGRAGKEGTAWSFVTPREMDHLHLIERVTR 363 ARGLD+SGVTHV N+D+PQDPESYVHRIGRTGRAGKEG A +FV PREM +L ++E T+ Sbjct: 301 ARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAGKEGMAITFVQPREMGYLRIVEETTK 360 Query: 364 HRITRKPLPTMAEAIEGKQRITAERLL-AMVEGGGELNEYKGIAIQLLEQYDSVQLLTAA 422 R+ PT EA G+ R+ E++ A+ E L +YK A +LLE+YD+ + A Sbjct: 361 KRMQPLQAPTWDEAFAGQLRVATEKIQEAITEEN--LADYKTFANELLEKYDATDIAAAM 418 Query: 423 MKLLTGDTKDAQVELTPEDPI 443 +K+L + V +T E P+ Sbjct: 419 LKMLAKEPDKTPVHITEERPL 439 >BH2384 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 539 Score = 472 bits (1214), Expect = e-133, Method: Compositional matrix adjust. Identities = 231/436 (52%), Positives = 327/436 (75%), Gaps = 8/436 (1%) Query: 7 FAEFGLEPKVLQAITELGFEEATPIQEQAIPIALTGSDLIGQAQTGTGKTAAFGIPLISK 66 F E + ++ +AI E+GFEE +PIQ +AIP L G D+IGQAQTGTGKTAAFGIP++ K Sbjct: 8 FNELQIGEEIKKAIIEMGFEEPSPIQAKAIPAILAGGDVIGQAQTGTGKTAAFGIPVVEK 67 Query: 67 IAREEEKILALVMTPTRELAIQVAEEIGKLTRFKGLRSLAIYGGQDIGRQIRGLKKKPQI 126 ++ + AL++TPTRELAIQV+ EI KL++ K +R+L IYGGQ I QI+ LK+ Q+ Sbjct: 68 VSTGRH-VQALILTPTRELAIQVSGEIQKLSKHKKIRTLPIYGGQSIVHQIKALKQGVQV 126 Query: 127 IIGTPGRLLDHINRKTIRLDDVQTIVLDEADEMLDMGFMEDIQTILKLVPEERQTMLFSA 186 +IGTPGR++DH+ RKT+ LD V T++LDEADEMLDMGF++DI++IL+ V ERQT+LFSA Sbjct: 127 VIGTPGRIIDHLRRKTLILDHVNTVILDEADEMLDMGFIDDIESILRQVKNERQTLLFSA 186 Query: 187 TMPPNIQRLAQQFLKNPQHVSVIPKQISAPLIDQAYIEVPERQKFEALSRLIDMESPDLA 246 TMPP I++L+++++ +PQ VS+ ++++AP IDQ Y +V ER K ++L R+ID E DL Sbjct: 187 TMPPAIKKLSRKYMNDPQTVSINRREVTAPSIDQFYYKVLERNKLDSLCRIIDSEQIDLG 246 Query: 247 IVFGRTKRRVDELAEGLQKRGYSADGLHGDLSQNQRDAVMRKFRDGSIDVLVATDVAARG 306 I+F RTK+ V EL E LQ RGY ADGLHGDL+Q+QRDAVMRKFRD SI+ L+ATDVAARG Sbjct: 247 ILFCRTKKGVAELTEALQARGYIADGLHGDLTQSQRDAVMRKFRDSSIEFLIATDVAARG 306 Query: 307 LDVSGVTHVINFDLPQDPESYVHRIGRTGRAGKEGTAWSFVTPREMDHLHLIERVTRHRI 366 +DV V+HVIN+D+PQDPESYVHRIGRTGRAG++G A + VTPREM HL IE+ + I Sbjct: 307 IDVGNVSHVINYDIPQDPESYVHRIGRTGRAGRKGLALTLVTPREMKHLRSIEQEIKMSI 366 Query: 367 TRKPLPTMAEAIEGKQRITAERLLAMVE-GGGELNEYKGIAIQLLEQYDSVQLLTAAMKL 425 + +PT+ + +E ++ +++ +E G + + ++ + +L+ +YDS ++++A ++L Sbjct: 367 PSQEVPTIEDVVEKQKESWKKQIAHRIEKGSNQKHLFEQMTEELMAEYDSKEVISALLQL 426 Query: 426 LTGDTKDAQVELTPED 441 + ++ PED Sbjct: 427 ------NYAMDTAPED 436 >SP1586 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 524 Score = 460 bits (1184), Expect = e-129, Method: Compositional matrix adjust. Identities = 215/383 (56%), Positives = 292/383 (76%) Query: 7 FAEFGLEPKVLQAITELGFEEATPIQEQAIPIALTGSDLIGQAQTGTGKTAAFGIPLISK 66 F E L +L I + GF EA+PIQEQ IP+AL G D+IGQAQTGTGKTAAFG+P + K Sbjct: 3 FNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQAQTGTGKTAAFGLPTLEK 62 Query: 67 IAREEEKILALVMTPTRELAIQVAEEIGKLTRFKGLRSLAIYGGQDIGRQIRGLKKKPQI 126 I EE I ALV+ PTRELA+Q EE+ + R KG++ ++YGG I +QI+ LK I Sbjct: 63 IRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRSVYGGSSIEKQIKALKSGAHI 122 Query: 127 IIGTPGRLLDHINRKTIRLDDVQTIVLDEADEMLDMGFMEDIQTILKLVPEERQTMLFSA 186 ++GTPGRLLD I RK ++L D++T++LDEADEML+MGF+EDI+ I+ VPE RQT+LFSA Sbjct: 123 VVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGFLEDIEAIISRVPENRQTLLFSA 182 Query: 187 TMPPNIQRLAQQFLKNPQHVSVIPKQISAPLIDQAYIEVPERQKFEALSRLIDMESPDLA 246 TMP I+R+ QF+K P+HV + K+++ L+DQ YI V E++KF+ ++RL+D+ P+LA Sbjct: 183 TMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYYIRVKEQEKFDTMTRLMDVAQPELA 242 Query: 247 IVFGRTKRRVDELAEGLQKRGYSADGLHGDLSQNQRDAVMRKFRDGSIDVLVATDVAARG 306 IVFGRTKRRVDEL GL+ RG+ A+G+HGDL QN+R V+R F++G++DVLVATDVAARG Sbjct: 243 IVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQNKRLRVLRDFKNGNLDVLVATDVAARG 302 Query: 307 LDVSGVTHVINFDLPQDPESYVHRIGRTGRAGKEGTAWSFVTPREMDHLHLIERVTRHRI 366 LD+SGVTHV N+D+PQDPESYVHRIGRTGRAGK G + +FV P EM +L +IE +T+ R+ Sbjct: 303 LDISGVTHVYNYDIPQDPESYVHRIGRTGRAGKSGQSITFVAPNEMGYLQIIENLTKKRM 362 Query: 367 TRKPLPTMAEAIEGKQRITAERL 389 ++ E+ + K+++ +++ Sbjct: 363 KGLKPASVEESFQSKKQVALKKI 385 >L0339 [LKJ] COG0513 Superfamily II DNA and RNA helicases Length = 547 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 229/444 (51%), Positives = 315/444 (70%), Gaps = 10/444 (2%) Query: 7 FAEFGLEPKVLQAITELGFEEATPIQEQAIPIALTGSDLIGQAQTGTGKTAAFGIPLISK 66 F+E GL ++ +TE+G+E+ TPIQEQ I +AL G D++GQAQTGTGKTAAFG+P I K Sbjct: 3 FSELGLSEGIVNTLTEIGYEQPTPIQEQTIKLALEGRDVLGQAQTGTGKTAAFGLPTIEK 62 Query: 67 IAREEEKILALVMTPTRELAIQVAEEIGKLTRFKGLRSLAIYGGQDIGRQIRGLKKKPQI 126 I I ALV+ PTRELA+Q EE+ + + KGL+ ++GG I +QI+GLK I Sbjct: 63 IDASNPAIQALVIAPTRELAVQGQEELFRFGKSKGLKVRTVFGGSSIEKQIKGLKAGAHI 122 Query: 127 IIGTPGRLLDHINRKTIRLDDVQTIVLDEADEMLDMGFMEDIQTILKLVPEERQTMLFSA 186 ++GTPGRL+D I RK I+LD ++T++LDEADEML+MGF+EDI I++ PE RQT+LFSA Sbjct: 123 VVGTPGRLVDLIKRKAIKLDQLETLILDEADEMLNMGFLEDIHFIIEKTPEARQTLLFSA 182 Query: 187 TMPPNIQRLAQQFLKNPQHVSVIPKQISAPLIDQAYIEVPERQKFEALSRLIDMESPDLA 246 TMP +I+++ +F+KNP+H+ + K+++A IDQ +++ E +KF+ L+RL+D+E P+LA Sbjct: 183 TMPNDIKKIGVKFMKNPEHIKIAAKEMTADRIDQYFVKTKEFEKFDVLTRLLDVERPELA 242 Query: 247 IVFGRTKRRVDELAEGLQKRGYSADGLHGDLSQNQRDAVMRKFRDGSIDVLVATDVAARG 306 IVFGRTKRRVDEL GL+ RGY A+G+HGDL QN+R AV+R F+ G IDVLVATDVAARG Sbjct: 243 IVFGRTKRRVDELIRGLKLRGYRAEGMHGDLDQNKRLAVLRDFKAGHIDVLVATDVAARG 302 Query: 307 LDVSGVTHVINFDLPQDPESYVHRIGRTGRAGKEGTAWSFVTPREMDHLHLIERVTRHRI 366 LDVSGVTHV N+D+ QD ESYVHRIGRTGRAGK G + +FV+ EM +L IE +T+ + Sbjct: 303 LDVSGVTHVYNYDITQDQESYVHRIGRTGRAGKSGRSVTFVSYNEMGYLRAIENMTKKPM 362 Query: 367 TRKPLPTMAEAIEGKQRITAE---RLLAMVEGGGELNEYKGIAIQLLEQYDSVQLLTAAM 423 PT EA + + + R L+ +L ++ A LLE++D+ +L+ Sbjct: 363 KGLKPPTKEEAYQASLSVAMDDVLRDLSDDSAKAKLAKFDKQAASLLEKFDAKELVAL-- 420 Query: 424 KLLTGDTKD----AQVELTPEDPI 443 LL G KD +V++T E P+ Sbjct: 421 -LLQGRVKDPDNQEEVKITAERPL 443 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,247,717 Number of extensions: 1474225 Number of successful extensions: 3896 Number of sequences better than 1.0e-05: 173 Number of HSP's gapped: 3626 Number of HSP's successfully gapped: 174 Length of query: 555 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 444 Effective length of database: 25,576,357 Effective search space: 11355902508 Effective search space used: 11355902508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)